Comparative analysis of the morphology, ultrastructure and glycosylation pattern of the jejunum and ileum of the wild rodent Lagostomus maximus

Autores
Tano de la Hoz, María Florencia; Flamini, Mirta Alicia; Díaz, Alcira Ofelia
Año de publicación
2016
Idioma
inglés
Tipo de recurso
artículo
Estado
versión publicada
Descripción
Morphological and histochemical analyses were performed to characterize the histology, ultrastructure, and glycosylation pattern of the jejunum and ileum of the wild rodent Lagostomus maximus. Enterocytes, goblet cells, Paneth cells, and enteroendocrine cells were identified in both intestinal epithelia. Two morphological types of enterocytes wereidentified only in the ileum based on their cytoplasm electron density. Although the histological and ultrastructural examination showed that the epithelia of both anatomical regions were morphologically similar, a certain specialization in their secretory products was evident. The glycosylation pattern of the jejunum and ileum was characterized in situ by histochemical and lectin histochemical methods. Histochemical results revealed the presence of carboxylated and sulfated gycoconjugates in both regions, although sulfomucins were clearly prevalent in the ileum. Sialic acid was highly O-acetylated and particularly abundant in the jejunum. The KOH/PA*/Bh/PAS technique evidenced a more intense histochemical reaction in the jejunal than in the ileum goblet cells, demonstrating a reduction of neutral mucin secretion in the distal small intestine. Further specific differences were revealed by lectin histochemistry. These data evidenced that the nature of mucus varies at different anatomical regions, probably adapted to physiological requirements.
Fil: Tano de la Hoz, María Florencia. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Mar del Plata. Instituto de Investigaciones Marinas y Costeras. Universidad Nacional de Mar del Plata. Facultad de Ciencias Exactas y Naturales. Instituto de Investigaciones Marinas y Costeras; Argentina
Fil: Flamini, Mirta Alicia. Universidad Nacional de La Plata. Facultad de Ciencias Veterinarias. Departamento de Ciencias Básicas. Cátedra de Histología y Embriología; Argentina
Fil: Díaz, Alcira Ofelia. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Mar del Plata. Instituto de Investigaciones Marinas y Costeras. Universidad Nacional de Mar del Plata. Facultad de Ciencias Exactas y Naturales. Instituto de Investigaciones Marinas y Costeras; Argentina
Materia
LAGOSTOMUS MAXIMUS
ULTRASTRUCTURE
SMALL INTESTINE
MORPHOLOGY
Nivel de accesibilidad
acceso abierto
Condiciones de uso
https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
Repositorio
CONICET Digital (CONICET)
Institución
Consejo Nacional de Investigaciones Científicas y Técnicas
OAI Identificador
oai:ri.conicet.gov.ar:11336/129693
Acceder
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oai_identifier_str oai:ri.conicet.gov.ar:11336/129693
network_acronym_str CONICETDig
repository_id_str 3498
network_name_str CONICET Digital (CONICET)
spelling Comparative analysis of the morphology, ultrastructure and glycosylation pattern of the jejunum and ileum of the wild rodent Lagostomus maximusTano de la Hoz, María FlorenciaFlamini, Mirta AliciaDíaz, Alcira OfeliaLAGOSTOMUS MAXIMUSULTRASTRUCTURESMALL INTESTINEMORPHOLOGYhttps://purl.org/becyt/ford/1.6https://purl.org/becyt/ford/1Morphological and histochemical analyses were performed to characterize the histology, ultrastructure, and glycosylation pattern of the jejunum and ileum of the wild rodent Lagostomus maximus. Enterocytes, goblet cells, Paneth cells, and enteroendocrine cells were identified in both intestinal epithelia. Two morphological types of enterocytes wereidentified only in the ileum based on their cytoplasm electron density. Although the histological and ultrastructural examination showed that the epithelia of both anatomical regions were morphologically similar, a certain specialization in their secretory products was evident. The glycosylation pattern of the jejunum and ileum was characterized in situ by histochemical and lectin histochemical methods. Histochemical results revealed the presence of carboxylated and sulfated gycoconjugates in both regions, although sulfomucins were clearly prevalent in the ileum. Sialic acid was highly O-acetylated and particularly abundant in the jejunum. The KOH/PA*/Bh/PAS technique evidenced a more intense histochemical reaction in the jejunal than in the ileum goblet cells, demonstrating a reduction of neutral mucin secretion in the distal small intestine. Further specific differences were revealed by lectin histochemistry. These data evidenced that the nature of mucus varies at different anatomical regions, probably adapted to physiological requirements.Fil: Tano de la Hoz, María Florencia. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Mar del Plata. Instituto de Investigaciones Marinas y Costeras. Universidad Nacional de Mar del Plata. Facultad de Ciencias Exactas y Naturales. Instituto de Investigaciones Marinas y Costeras; ArgentinaFil: Flamini, Mirta Alicia. Universidad Nacional de La Plata. Facultad de Ciencias Veterinarias. Departamento de Ciencias Básicas. Cátedra de Histología y Embriología; ArgentinaFil: Díaz, Alcira Ofelia. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Mar del Plata. Instituto de Investigaciones Marinas y Costeras. Universidad Nacional de Mar del Plata. Facultad de Ciencias Exactas y Naturales. Instituto de Investigaciones Marinas y Costeras; ArgentinaVeterinary and Human Toxicology2016-02info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionhttp://purl.org/coar/resource_type/c_6501info:ar-repo/semantics/articuloapplication/pdfapplication/pdfapplication/pdfhttp://hdl.handle.net/11336/129693Tano de la Hoz, María Florencia; Flamini, Mirta Alicia; Díaz, Alcira Ofelia; Comparative analysis of the morphology, ultrastructure and glycosylation pattern of the jejunum and ileum of the wild rodent Lagostomus maximus; Veterinary and Human Toxicology; Anatomical Record-Advances in Integrative Anatomy and Evolutionary Biology; 299; 5; 2-2016; 630-6421932-8486CONICET DigitalCONICETenginfo:eu-repo/semantics/altIdentifier/url/https://anatomypubs.onlinelibrary.wiley.com/doi/full/10.1002/ar.23335info:eu-repo/semantics/altIdentifier/doi/10.1002/ar.23335info:eu-repo/semantics/openAccesshttps://creativecommons.org/licenses/by-nc-sa/2.5/ar/reponame:CONICET Digital (CONICET)instname:Consejo Nacional de Investigaciones Científicas y Técnicas2025-09-03T10:10:06Zoai:ri.conicet.gov.ar:11336/129693instacron:CONICETInstitucionalhttp://ri.conicet.gov.ar/Organismo científico-tecnológicoNo correspondehttp://ri.conicet.gov.ar/oai/requestdasensio@conicet.gov.ar; lcarlino@conicet.gov.arArgentinaNo correspondeNo correspondeNo correspondeopendoar:34982025-09-03 10:10:06.872CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicasfalse
dc.title.none.fl_str_mv Comparative analysis of the morphology, ultrastructure and glycosylation pattern of the jejunum and ileum of the wild rodent Lagostomus maximus
title Comparative analysis of the morphology, ultrastructure and glycosylation pattern of the jejunum and ileum of the wild rodent Lagostomus maximus
spellingShingle Comparative analysis of the morphology, ultrastructure and glycosylation pattern of the jejunum and ileum of the wild rodent Lagostomus maximus
Tano de la Hoz, María Florencia
LAGOSTOMUS MAXIMUS
ULTRASTRUCTURE
SMALL INTESTINE
MORPHOLOGY
title_short Comparative analysis of the morphology, ultrastructure and glycosylation pattern of the jejunum and ileum of the wild rodent Lagostomus maximus
title_full Comparative analysis of the morphology, ultrastructure and glycosylation pattern of the jejunum and ileum of the wild rodent Lagostomus maximus
title_fullStr Comparative analysis of the morphology, ultrastructure and glycosylation pattern of the jejunum and ileum of the wild rodent Lagostomus maximus
title_full_unstemmed Comparative analysis of the morphology, ultrastructure and glycosylation pattern of the jejunum and ileum of the wild rodent Lagostomus maximus
title_sort Comparative analysis of the morphology, ultrastructure and glycosylation pattern of the jejunum and ileum of the wild rodent Lagostomus maximus
dc.creator.none.fl_str_mv Tano de la Hoz, María Florencia
Flamini, Mirta Alicia
Díaz, Alcira Ofelia
author Tano de la Hoz, María Florencia
author_facet Tano de la Hoz, María Florencia
Flamini, Mirta Alicia
Díaz, Alcira Ofelia
author_role author
author2 Flamini, Mirta Alicia
Díaz, Alcira Ofelia
author2_role author
author
dc.subject.none.fl_str_mv LAGOSTOMUS MAXIMUS
ULTRASTRUCTURE
SMALL INTESTINE
MORPHOLOGY
topic LAGOSTOMUS MAXIMUS
ULTRASTRUCTURE
SMALL INTESTINE
MORPHOLOGY
purl_subject.fl_str_mv https://purl.org/becyt/ford/1.6
https://purl.org/becyt/ford/1
dc.description.none.fl_txt_mv Morphological and histochemical analyses were performed to characterize the histology, ultrastructure, and glycosylation pattern of the jejunum and ileum of the wild rodent Lagostomus maximus. Enterocytes, goblet cells, Paneth cells, and enteroendocrine cells were identified in both intestinal epithelia. Two morphological types of enterocytes wereidentified only in the ileum based on their cytoplasm electron density. Although the histological and ultrastructural examination showed that the epithelia of both anatomical regions were morphologically similar, a certain specialization in their secretory products was evident. The glycosylation pattern of the jejunum and ileum was characterized in situ by histochemical and lectin histochemical methods. Histochemical results revealed the presence of carboxylated and sulfated gycoconjugates in both regions, although sulfomucins were clearly prevalent in the ileum. Sialic acid was highly O-acetylated and particularly abundant in the jejunum. The KOH/PA*/Bh/PAS technique evidenced a more intense histochemical reaction in the jejunal than in the ileum goblet cells, demonstrating a reduction of neutral mucin secretion in the distal small intestine. Further specific differences were revealed by lectin histochemistry. These data evidenced that the nature of mucus varies at different anatomical regions, probably adapted to physiological requirements.
Fil: Tano de la Hoz, María Florencia. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Mar del Plata. Instituto de Investigaciones Marinas y Costeras. Universidad Nacional de Mar del Plata. Facultad de Ciencias Exactas y Naturales. Instituto de Investigaciones Marinas y Costeras; Argentina
Fil: Flamini, Mirta Alicia. Universidad Nacional de La Plata. Facultad de Ciencias Veterinarias. Departamento de Ciencias Básicas. Cátedra de Histología y Embriología; Argentina
Fil: Díaz, Alcira Ofelia. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Mar del Plata. Instituto de Investigaciones Marinas y Costeras. Universidad Nacional de Mar del Plata. Facultad de Ciencias Exactas y Naturales. Instituto de Investigaciones Marinas y Costeras; Argentina
description Morphological and histochemical analyses were performed to characterize the histology, ultrastructure, and glycosylation pattern of the jejunum and ileum of the wild rodent Lagostomus maximus. Enterocytes, goblet cells, Paneth cells, and enteroendocrine cells were identified in both intestinal epithelia. Two morphological types of enterocytes wereidentified only in the ileum based on their cytoplasm electron density. Although the histological and ultrastructural examination showed that the epithelia of both anatomical regions were morphologically similar, a certain specialization in their secretory products was evident. The glycosylation pattern of the jejunum and ileum was characterized in situ by histochemical and lectin histochemical methods. Histochemical results revealed the presence of carboxylated and sulfated gycoconjugates in both regions, although sulfomucins were clearly prevalent in the ileum. Sialic acid was highly O-acetylated and particularly abundant in the jejunum. The KOH/PA*/Bh/PAS technique evidenced a more intense histochemical reaction in the jejunal than in the ileum goblet cells, demonstrating a reduction of neutral mucin secretion in the distal small intestine. Further specific differences were revealed by lectin histochemistry. These data evidenced that the nature of mucus varies at different anatomical regions, probably adapted to physiological requirements.
publishDate 2016
dc.date.none.fl_str_mv 2016-02
dc.type.none.fl_str_mv info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
http://purl.org/coar/resource_type/c_6501
info:ar-repo/semantics/articulo
format article
status_str publishedVersion
dc.identifier.none.fl_str_mv http://hdl.handle.net/11336/129693
Tano de la Hoz, María Florencia; Flamini, Mirta Alicia; Díaz, Alcira Ofelia; Comparative analysis of the morphology, ultrastructure and glycosylation pattern of the jejunum and ileum of the wild rodent Lagostomus maximus; Veterinary and Human Toxicology; Anatomical Record-Advances in Integrative Anatomy and Evolutionary Biology; 299; 5; 2-2016; 630-642
1932-8486
CONICET Digital
CONICET
url http://hdl.handle.net/11336/129693
identifier_str_mv Tano de la Hoz, María Florencia; Flamini, Mirta Alicia; Díaz, Alcira Ofelia; Comparative analysis of the morphology, ultrastructure and glycosylation pattern of the jejunum and ileum of the wild rodent Lagostomus maximus; Veterinary and Human Toxicology; Anatomical Record-Advances in Integrative Anatomy and Evolutionary Biology; 299; 5; 2-2016; 630-642
1932-8486
CONICET Digital
CONICET
dc.language.none.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv info:eu-repo/semantics/altIdentifier/url/https://anatomypubs.onlinelibrary.wiley.com/doi/full/10.1002/ar.23335
info:eu-repo/semantics/altIdentifier/doi/10.1002/ar.23335
dc.rights.none.fl_str_mv info:eu-repo/semantics/openAccess
https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
eu_rights_str_mv openAccess
rights_invalid_str_mv https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
dc.format.none.fl_str_mv application/pdf
application/pdf
application/pdf
dc.publisher.none.fl_str_mv Veterinary and Human Toxicology
publisher.none.fl_str_mv Veterinary and Human Toxicology
dc.source.none.fl_str_mv reponame:CONICET Digital (CONICET)
instname:Consejo Nacional de Investigaciones Científicas y Técnicas
reponame_str CONICET Digital (CONICET)
collection CONICET Digital (CONICET)
instname_str Consejo Nacional de Investigaciones Científicas y Técnicas
repository.name.fl_str_mv CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicas
repository.mail.fl_str_mv dasensio@conicet.gov.ar; lcarlino@conicet.gov.ar
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score 13.13397

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