Characterization of dendritic cells in testicular draining lymph nodes in a rat model of experimental autoimmune orchitis

Autores
Guazzone, Vanesa Anabella; Hollwegs, S.; Mardirosian, Mariana Noelia; Jacobo, Patricia Verónica; Hackstein, H.; Wygrecka, M.; Schneider, E.; Meinhardt, A.; Lustig, Livia; Fijak M.
Año de publicación
2011
Idioma
inglés
Tipo de recurso
artículo
Estado
versión publicada
Descripción
The maturation state of dendritic cells (DC) is regarded as a control point for the induction of peripheral tolerance or autoimmunity. Experimental autoimmune orchitis (EAO) serves as a model to investigate inflammatory-based testicular impairment, which ranks as a significant cause of male infertility. This work aimed to determine whether DC enrichment occurs organotypically in testicular draining lymph nodes (TLN) compared with LN draining the site of immunization (ILN) and thus contributes to the pathogenesis of autoimmune orchitis. In this regard, we quantified and characterized the DC from TLN and ILN in rats with EAO. Flow cytometric analysis showed a significant increase in the percentage of DC (OX62+) only in TLN from EAO rats compared with normal (N) and adjuvant control (C) groups. The number of DC from ILN and TLN expressing CD80, CD86 and major histocompatibility complex (MHC) II was comparable among N, C and experimental (E) groups at 30 and 50days after the first immunization. However, TLN DC from EAO rats (50days) showed an increase in mean fluorescence intensity for MHC II compared with N, C and E groups (30days). The mRNA expression level of IL-10 and IL-12p35 was significantly upregulated in enriched DC fraction from TLN in EAO rats with no significant changes observed in ILN DC. The expression of IL-23p19 mRNA remained unchanged. Functional data, using proliferation assays showed that EAO-DC from TLN, but not from ILN, significantly enhanced the proliferation of naïve T cells compared with C-DC. In summary, our data suggest that the DC in TLN from orchitis rats are mature, present antigens to T cells and stimulate an autoimmune response against testicular antigens, thus causing immunological infertility.
Fil: Guazzone, Vanesa Anabella. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Houssay. Instituto de Investigaciones Biomédicas. Universidad de Buenos Aires. Facultad de Medicina. Instituto de Investigaciones Biomédicas; Argentina
Fil: Hollwegs, S.. University of Giessen; Alemania
Fil: Mardirosian, Mariana Noelia. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Houssay. Instituto de Investigaciones Biomédicas. Universidad de Buenos Aires. Facultad de Medicina. Instituto de Investigaciones Biomédicas; Argentina
Fil: Jacobo, Patricia Verónica. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Houssay. Instituto de Investigaciones Biomédicas. Universidad de Buenos Aires. Facultad de Medicina. Instituto de Investigaciones Biomédicas; Argentina
Fil: Hackstein, H.. University of Giessen; Alemania
Fil: Wygrecka, M.. University of Giessen; Alemania
Fil: Schneider, E.. University of Giessen; Alemania
Fil: Meinhardt, A.. University of Giessen; Alemania
Fil: Lustig, Livia. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Houssay. Instituto de Investigaciones Biomédicas. Universidad de Buenos Aires. Facultad de Medicina. Instituto de Investigaciones Biomédicas; Argentina
Fil: Fijak M.. University of Giessen; Alemania
Materia
CO-STIMULATORY MOLECULES
CYTOKINES
DENDRITIC CELLS
EXPERIMENTAL AUTOIMMUNE ORCHITIS
LYMPH NODES
TESTICULAR INFLAMMATION
Nivel de accesibilidad
acceso abierto
Condiciones de uso
https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
Repositorio
CONICET Digital (CONICET)
Institución
Consejo Nacional de Investigaciones Científicas y Técnicas
OAI Identificador
oai:ri.conicet.gov.ar:11336/102754
Acceder
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oai_identifier_str oai:ri.conicet.gov.ar:11336/102754
network_acronym_str CONICETDig
repository_id_str 3498
network_name_str CONICET Digital (CONICET)
spelling Characterization of dendritic cells in testicular draining lymph nodes in a rat model of experimental autoimmune orchitisGuazzone, Vanesa AnabellaHollwegs, S.Mardirosian, Mariana NoeliaJacobo, Patricia VerónicaHackstein, H.Wygrecka, M.Schneider, E.Meinhardt, A.Lustig, LiviaFijak M.CO-STIMULATORY MOLECULESCYTOKINESDENDRITIC CELLSEXPERIMENTAL AUTOIMMUNE ORCHITISLYMPH NODESTESTICULAR INFLAMMATIONhttps://purl.org/becyt/ford/3.1https://purl.org/becyt/ford/3The maturation state of dendritic cells (DC) is regarded as a control point for the induction of peripheral tolerance or autoimmunity. Experimental autoimmune orchitis (EAO) serves as a model to investigate inflammatory-based testicular impairment, which ranks as a significant cause of male infertility. This work aimed to determine whether DC enrichment occurs organotypically in testicular draining lymph nodes (TLN) compared with LN draining the site of immunization (ILN) and thus contributes to the pathogenesis of autoimmune orchitis. In this regard, we quantified and characterized the DC from TLN and ILN in rats with EAO. Flow cytometric analysis showed a significant increase in the percentage of DC (OX62+) only in TLN from EAO rats compared with normal (N) and adjuvant control (C) groups. The number of DC from ILN and TLN expressing CD80, CD86 and major histocompatibility complex (MHC) II was comparable among N, C and experimental (E) groups at 30 and 50days after the first immunization. However, TLN DC from EAO rats (50days) showed an increase in mean fluorescence intensity for MHC II compared with N, C and E groups (30days). The mRNA expression level of IL-10 and IL-12p35 was significantly upregulated in enriched DC fraction from TLN in EAO rats with no significant changes observed in ILN DC. The expression of IL-23p19 mRNA remained unchanged. Functional data, using proliferation assays showed that EAO-DC from TLN, but not from ILN, significantly enhanced the proliferation of naïve T cells compared with C-DC. In summary, our data suggest that the DC in TLN from orchitis rats are mature, present antigens to T cells and stimulate an autoimmune response against testicular antigens, thus causing immunological infertility.Fil: Guazzone, Vanesa Anabella. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Houssay. Instituto de Investigaciones Biomédicas. Universidad de Buenos Aires. Facultad de Medicina. Instituto de Investigaciones Biomédicas; ArgentinaFil: Hollwegs, S.. University of Giessen; AlemaniaFil: Mardirosian, Mariana Noelia. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Houssay. Instituto de Investigaciones Biomédicas. Universidad de Buenos Aires. Facultad de Medicina. Instituto de Investigaciones Biomédicas; ArgentinaFil: Jacobo, Patricia Verónica. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Houssay. Instituto de Investigaciones Biomédicas. Universidad de Buenos Aires. Facultad de Medicina. Instituto de Investigaciones Biomédicas; ArgentinaFil: Hackstein, H.. University of Giessen; AlemaniaFil: Wygrecka, M.. University of Giessen; AlemaniaFil: Schneider, E.. University of Giessen; AlemaniaFil: Meinhardt, A.. University of Giessen; AlemaniaFil: Lustig, Livia. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Houssay. Instituto de Investigaciones Biomédicas. Universidad de Buenos Aires. Facultad de Medicina. Instituto de Investigaciones Biomédicas; ArgentinaFil: Fijak M.. University of Giessen; AlemaniaWiley Blackwell Publishing, Inc2011-06info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionhttp://purl.org/coar/resource_type/c_6501info:ar-repo/semantics/articuloapplication/pdfapplication/pdfapplication/pdfapplication/pdfapplication/pdfhttp://hdl.handle.net/11336/102754Guazzone, Vanesa Anabella; Hollwegs, S.; Mardirosian, Mariana Noelia; Jacobo, Patricia Verónica; Hackstein, H.; et al.; Characterization of dendritic cells in testicular draining lymph nodes in a rat model of experimental autoimmune orchitis; Wiley Blackwell Publishing, Inc; International Journal Of Andrology; 34; 3; 6-2011; 276-2890105-6263CONICET DigitalCONICETenginfo:eu-repo/semantics/altIdentifier/url/https://onlinelibrary.wiley.com/doi/full/10.1111/j.1365-2605.2010.01082.xinfo:eu-repo/semantics/altIdentifier/doi/10.1111/j.1365-2605.2010.01082.xinfo:eu-repo/semantics/openAccesshttps://creativecommons.org/licenses/by-nc-sa/2.5/ar/reponame:CONICET Digital (CONICET)instname:Consejo Nacional de Investigaciones Científicas y Técnicas2025-09-03T10:10:54Zoai:ri.conicet.gov.ar:11336/102754instacron:CONICETInstitucionalhttp://ri.conicet.gov.ar/Organismo científico-tecnológicoNo correspondehttp://ri.conicet.gov.ar/oai/requestdasensio@conicet.gov.ar; lcarlino@conicet.gov.arArgentinaNo correspondeNo correspondeNo correspondeopendoar:34982025-09-03 10:10:54.955CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicasfalse
dc.title.none.fl_str_mv Characterization of dendritic cells in testicular draining lymph nodes in a rat model of experimental autoimmune orchitis
title Characterization of dendritic cells in testicular draining lymph nodes in a rat model of experimental autoimmune orchitis
spellingShingle Characterization of dendritic cells in testicular draining lymph nodes in a rat model of experimental autoimmune orchitis
Guazzone, Vanesa Anabella
CO-STIMULATORY MOLECULES
CYTOKINES
DENDRITIC CELLS
EXPERIMENTAL AUTOIMMUNE ORCHITIS
LYMPH NODES
TESTICULAR INFLAMMATION
title_short Characterization of dendritic cells in testicular draining lymph nodes in a rat model of experimental autoimmune orchitis
title_full Characterization of dendritic cells in testicular draining lymph nodes in a rat model of experimental autoimmune orchitis
title_fullStr Characterization of dendritic cells in testicular draining lymph nodes in a rat model of experimental autoimmune orchitis
title_full_unstemmed Characterization of dendritic cells in testicular draining lymph nodes in a rat model of experimental autoimmune orchitis
title_sort Characterization of dendritic cells in testicular draining lymph nodes in a rat model of experimental autoimmune orchitis
dc.creator.none.fl_str_mv Guazzone, Vanesa Anabella
Hollwegs, S.
Mardirosian, Mariana Noelia
Jacobo, Patricia Verónica
Hackstein, H.
Wygrecka, M.
Schneider, E.
Meinhardt, A.
Lustig, Livia
Fijak M.
author Guazzone, Vanesa Anabella
author_facet Guazzone, Vanesa Anabella
Hollwegs, S.
Mardirosian, Mariana Noelia
Jacobo, Patricia Verónica
Hackstein, H.
Wygrecka, M.
Schneider, E.
Meinhardt, A.
Lustig, Livia
Fijak M.
author_role author
author2 Hollwegs, S.
Mardirosian, Mariana Noelia
Jacobo, Patricia Verónica
Hackstein, H.
Wygrecka, M.
Schneider, E.
Meinhardt, A.
Lustig, Livia
Fijak M.
author2_role author
author
author
author
author
author
author
author
author
dc.subject.none.fl_str_mv CO-STIMULATORY MOLECULES
CYTOKINES
DENDRITIC CELLS
EXPERIMENTAL AUTOIMMUNE ORCHITIS
LYMPH NODES
TESTICULAR INFLAMMATION
topic CO-STIMULATORY MOLECULES
CYTOKINES
DENDRITIC CELLS
EXPERIMENTAL AUTOIMMUNE ORCHITIS
LYMPH NODES
TESTICULAR INFLAMMATION
purl_subject.fl_str_mv https://purl.org/becyt/ford/3.1
https://purl.org/becyt/ford/3
dc.description.none.fl_txt_mv The maturation state of dendritic cells (DC) is regarded as a control point for the induction of peripheral tolerance or autoimmunity. Experimental autoimmune orchitis (EAO) serves as a model to investigate inflammatory-based testicular impairment, which ranks as a significant cause of male infertility. This work aimed to determine whether DC enrichment occurs organotypically in testicular draining lymph nodes (TLN) compared with LN draining the site of immunization (ILN) and thus contributes to the pathogenesis of autoimmune orchitis. In this regard, we quantified and characterized the DC from TLN and ILN in rats with EAO. Flow cytometric analysis showed a significant increase in the percentage of DC (OX62+) only in TLN from EAO rats compared with normal (N) and adjuvant control (C) groups. The number of DC from ILN and TLN expressing CD80, CD86 and major histocompatibility complex (MHC) II was comparable among N, C and experimental (E) groups at 30 and 50days after the first immunization. However, TLN DC from EAO rats (50days) showed an increase in mean fluorescence intensity for MHC II compared with N, C and E groups (30days). The mRNA expression level of IL-10 and IL-12p35 was significantly upregulated in enriched DC fraction from TLN in EAO rats with no significant changes observed in ILN DC. The expression of IL-23p19 mRNA remained unchanged. Functional data, using proliferation assays showed that EAO-DC from TLN, but not from ILN, significantly enhanced the proliferation of naïve T cells compared with C-DC. In summary, our data suggest that the DC in TLN from orchitis rats are mature, present antigens to T cells and stimulate an autoimmune response against testicular antigens, thus causing immunological infertility.
Fil: Guazzone, Vanesa Anabella. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Houssay. Instituto de Investigaciones Biomédicas. Universidad de Buenos Aires. Facultad de Medicina. Instituto de Investigaciones Biomédicas; Argentina
Fil: Hollwegs, S.. University of Giessen; Alemania
Fil: Mardirosian, Mariana Noelia. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Houssay. Instituto de Investigaciones Biomédicas. Universidad de Buenos Aires. Facultad de Medicina. Instituto de Investigaciones Biomédicas; Argentina
Fil: Jacobo, Patricia Verónica. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Houssay. Instituto de Investigaciones Biomédicas. Universidad de Buenos Aires. Facultad de Medicina. Instituto de Investigaciones Biomédicas; Argentina
Fil: Hackstein, H.. University of Giessen; Alemania
Fil: Wygrecka, M.. University of Giessen; Alemania
Fil: Schneider, E.. University of Giessen; Alemania
Fil: Meinhardt, A.. University of Giessen; Alemania
Fil: Lustig, Livia. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Houssay. Instituto de Investigaciones Biomédicas. Universidad de Buenos Aires. Facultad de Medicina. Instituto de Investigaciones Biomédicas; Argentina
Fil: Fijak M.. University of Giessen; Alemania
description The maturation state of dendritic cells (DC) is regarded as a control point for the induction of peripheral tolerance or autoimmunity. Experimental autoimmune orchitis (EAO) serves as a model to investigate inflammatory-based testicular impairment, which ranks as a significant cause of male infertility. This work aimed to determine whether DC enrichment occurs organotypically in testicular draining lymph nodes (TLN) compared with LN draining the site of immunization (ILN) and thus contributes to the pathogenesis of autoimmune orchitis. In this regard, we quantified and characterized the DC from TLN and ILN in rats with EAO. Flow cytometric analysis showed a significant increase in the percentage of DC (OX62+) only in TLN from EAO rats compared with normal (N) and adjuvant control (C) groups. The number of DC from ILN and TLN expressing CD80, CD86 and major histocompatibility complex (MHC) II was comparable among N, C and experimental (E) groups at 30 and 50days after the first immunization. However, TLN DC from EAO rats (50days) showed an increase in mean fluorescence intensity for MHC II compared with N, C and E groups (30days). The mRNA expression level of IL-10 and IL-12p35 was significantly upregulated in enriched DC fraction from TLN in EAO rats with no significant changes observed in ILN DC. The expression of IL-23p19 mRNA remained unchanged. Functional data, using proliferation assays showed that EAO-DC from TLN, but not from ILN, significantly enhanced the proliferation of naïve T cells compared with C-DC. In summary, our data suggest that the DC in TLN from orchitis rats are mature, present antigens to T cells and stimulate an autoimmune response against testicular antigens, thus causing immunological infertility.
publishDate 2011
dc.date.none.fl_str_mv 2011-06
dc.type.none.fl_str_mv info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
http://purl.org/coar/resource_type/c_6501
info:ar-repo/semantics/articulo
format article
status_str publishedVersion
dc.identifier.none.fl_str_mv http://hdl.handle.net/11336/102754
Guazzone, Vanesa Anabella; Hollwegs, S.; Mardirosian, Mariana Noelia; Jacobo, Patricia Verónica; Hackstein, H.; et al.; Characterization of dendritic cells in testicular draining lymph nodes in a rat model of experimental autoimmune orchitis; Wiley Blackwell Publishing, Inc; International Journal Of Andrology; 34; 3; 6-2011; 276-289
0105-6263
CONICET Digital
CONICET
url http://hdl.handle.net/11336/102754
identifier_str_mv Guazzone, Vanesa Anabella; Hollwegs, S.; Mardirosian, Mariana Noelia; Jacobo, Patricia Verónica; Hackstein, H.; et al.; Characterization of dendritic cells in testicular draining lymph nodes in a rat model of experimental autoimmune orchitis; Wiley Blackwell Publishing, Inc; International Journal Of Andrology; 34; 3; 6-2011; 276-289
0105-6263
CONICET Digital
CONICET
dc.language.none.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv info:eu-repo/semantics/altIdentifier/url/https://onlinelibrary.wiley.com/doi/full/10.1111/j.1365-2605.2010.01082.x
info:eu-repo/semantics/altIdentifier/doi/10.1111/j.1365-2605.2010.01082.x
dc.rights.none.fl_str_mv info:eu-repo/semantics/openAccess
https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
eu_rights_str_mv openAccess
rights_invalid_str_mv https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
dc.format.none.fl_str_mv application/pdf
application/pdf
application/pdf
application/pdf
application/pdf
dc.publisher.none.fl_str_mv Wiley Blackwell Publishing, Inc
publisher.none.fl_str_mv Wiley Blackwell Publishing, Inc
dc.source.none.fl_str_mv reponame:CONICET Digital (CONICET)
instname:Consejo Nacional de Investigaciones Científicas y Técnicas
reponame_str CONICET Digital (CONICET)
collection CONICET Digital (CONICET)
instname_str Consejo Nacional de Investigaciones Científicas y Técnicas
repository.name.fl_str_mv CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicas
repository.mail.fl_str_mv dasensio@conicet.gov.ar; lcarlino@conicet.gov.ar
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