Evidence of microbial rhodopsins in Antarctic Dry Valley edaphic systems

Autores
Guerrero, Leandro Demián; Vikram, Surendra; Makhalanyane, Thulani P.; Cowan, Don A.
Año de publicación
2017
Idioma
inglés
Tipo de recurso
artículo
Estado
versión publicada
Descripción
Microorganisms able to synthesize rhodopsins have the capacity to translocate ions through their membranes, using solar energy to generate a proton motive force. Rhodopsins are the most abundant phototrophic proteins in oceanic surface waters and are key constituents in marine bacterial ecology. However, it remains unclear how rhodopsins are used in most microorganisms. Despite their abundance in marine and fresh-water systems, the presence of functional rhodopsin systems in edaphic habitats has never been reported. Here, we show the presence of several new putative H+, Na+ and Cl+ pumping rhodopsins identified by metagenomic analysis of Antarctic desert hypolithic communities. Reconstruction of two Proteobacteria genomes harboring xanthorhodopsin-like proteins and one Bacteroidetes genome with a Na-pumping-like rhodopsin indicated that these bacteria were aerobic heterotrophs possessing the apparent capacity for the functional expression of rhodopsins. The existence of these protein systems in hypolithic bacteria expands the known role of rhodopsins to include terrestrial environments and suggests a possible predominant function as heterotrophic energy supply proteins, a feasible microbial adaptation to the harsh conditions prevalent in Antarctic edaphic systems.
Fil: Guerrero, Leandro Demián. Consejo Nacional de Investigaciones Científicas y Técnicas. Instituto de Investigaciones en Ingeniería Genética y Biología Molecular "Dr. Héctor N. Torres"; Argentina. University of Pretoria. Department of Genetics. Centre of Microbial Ecology and Genomics; Sudáfrica
Fil: Vikram, Surendra. University of Pretoria. Department of Genetics. Centre of Microbial Ecology and Genomics; Sudáfrica
Fil: Makhalanyane, Thulani P.. University of Pretoria. Department of Genetics. Centre of Microbial Ecology and Genomics; Sudáfrica
Fil: Cowan, Don A.. University of Pretoria. Department of Genetics. Centre of Microbial Ecology and Genomics; Sudáfrica
Materia
Rhodopsins
Antarctica
Metagenimics
Soil
Nivel de accesibilidad
acceso abierto
Condiciones de uso
https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
Repositorio
CONICET Digital (CONICET)
Institución
Consejo Nacional de Investigaciones Científicas y Técnicas
OAI Identificador
oai:ri.conicet.gov.ar:11336/72604
Acceder
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oai_identifier_str oai:ri.conicet.gov.ar:11336/72604
network_acronym_str CONICETDig
repository_id_str 3498
network_name_str CONICET Digital (CONICET)
spelling Evidence of microbial rhodopsins in Antarctic Dry Valley edaphic systemsGuerrero, Leandro DemiánVikram, SurendraMakhalanyane, Thulani P.Cowan, Don A.RhodopsinsAntarcticaMetagenimicsSoilhttps://purl.org/becyt/ford/1.6https://purl.org/becyt/ford/1Microorganisms able to synthesize rhodopsins have the capacity to translocate ions through their membranes, using solar energy to generate a proton motive force. Rhodopsins are the most abundant phototrophic proteins in oceanic surface waters and are key constituents in marine bacterial ecology. However, it remains unclear how rhodopsins are used in most microorganisms. Despite their abundance in marine and fresh-water systems, the presence of functional rhodopsin systems in edaphic habitats has never been reported. Here, we show the presence of several new putative H+, Na+ and Cl+ pumping rhodopsins identified by metagenomic analysis of Antarctic desert hypolithic communities. Reconstruction of two Proteobacteria genomes harboring xanthorhodopsin-like proteins and one Bacteroidetes genome with a Na-pumping-like rhodopsin indicated that these bacteria were aerobic heterotrophs possessing the apparent capacity for the functional expression of rhodopsins. The existence of these protein systems in hypolithic bacteria expands the known role of rhodopsins to include terrestrial environments and suggests a possible predominant function as heterotrophic energy supply proteins, a feasible microbial adaptation to the harsh conditions prevalent in Antarctic edaphic systems.Fil: Guerrero, Leandro Demián. Consejo Nacional de Investigaciones Científicas y Técnicas. Instituto de Investigaciones en Ingeniería Genética y Biología Molecular "Dr. Héctor N. Torres"; Argentina. University of Pretoria. Department of Genetics. Centre of Microbial Ecology and Genomics; SudáfricaFil: Vikram, Surendra. University of Pretoria. Department of Genetics. Centre of Microbial Ecology and Genomics; SudáfricaFil: Makhalanyane, Thulani P.. University of Pretoria. Department of Genetics. Centre of Microbial Ecology and Genomics; SudáfricaFil: Cowan, Don A.. University of Pretoria. Department of Genetics. Centre of Microbial Ecology and Genomics; SudáfricaWiley Blackwell Publishing, Inc2017-09info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionhttp://purl.org/coar/resource_type/c_6501info:ar-repo/semantics/articuloapplication/pdfapplication/pdfhttp://hdl.handle.net/11336/72604Guerrero, Leandro Demián; Vikram, Surendra; Makhalanyane, Thulani P.; Cowan, Don A.; Evidence of microbial rhodopsins in Antarctic Dry Valley edaphic systems; Wiley Blackwell Publishing, Inc; Environmental Microbiology; 19; 9; 9-2017; 3755-37671462-2912CONICET DigitalCONICETenginfo:eu-repo/semantics/altIdentifier/url/https://onlinelibrary.wiley.com/doi/abs/10.1111/1462-2920.13877info:eu-repo/semantics/altIdentifier/doi/10.1111/1462-2920.13877info:eu-repo/semantics/openAccesshttps://creativecommons.org/licenses/by-nc-sa/2.5/ar/reponame:CONICET Digital (CONICET)instname:Consejo Nacional de Investigaciones Científicas y Técnicas2025-09-29T09:55:35Zoai:ri.conicet.gov.ar:11336/72604instacron:CONICETInstitucionalhttp://ri.conicet.gov.ar/Organismo científico-tecnológicoNo correspondehttp://ri.conicet.gov.ar/oai/requestdasensio@conicet.gov.ar; lcarlino@conicet.gov.arArgentinaNo correspondeNo correspondeNo correspondeopendoar:34982025-09-29 09:55:35.269CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicasfalse
dc.title.none.fl_str_mv Evidence of microbial rhodopsins in Antarctic Dry Valley edaphic systems
title Evidence of microbial rhodopsins in Antarctic Dry Valley edaphic systems
spellingShingle Evidence of microbial rhodopsins in Antarctic Dry Valley edaphic systems
Guerrero, Leandro Demián
Rhodopsins
Antarctica
Metagenimics
Soil
title_short Evidence of microbial rhodopsins in Antarctic Dry Valley edaphic systems
title_full Evidence of microbial rhodopsins in Antarctic Dry Valley edaphic systems
title_fullStr Evidence of microbial rhodopsins in Antarctic Dry Valley edaphic systems
title_full_unstemmed Evidence of microbial rhodopsins in Antarctic Dry Valley edaphic systems
title_sort Evidence of microbial rhodopsins in Antarctic Dry Valley edaphic systems
dc.creator.none.fl_str_mv Guerrero, Leandro Demián
Vikram, Surendra
Makhalanyane, Thulani P.
Cowan, Don A.
author Guerrero, Leandro Demián
author_facet Guerrero, Leandro Demián
Vikram, Surendra
Makhalanyane, Thulani P.
Cowan, Don A.
author_role author
author2 Vikram, Surendra
Makhalanyane, Thulani P.
Cowan, Don A.
author2_role author
author
author
dc.subject.none.fl_str_mv Rhodopsins
Antarctica
Metagenimics
Soil
topic Rhodopsins
Antarctica
Metagenimics
Soil
purl_subject.fl_str_mv https://purl.org/becyt/ford/1.6
https://purl.org/becyt/ford/1
dc.description.none.fl_txt_mv Microorganisms able to synthesize rhodopsins have the capacity to translocate ions through their membranes, using solar energy to generate a proton motive force. Rhodopsins are the most abundant phototrophic proteins in oceanic surface waters and are key constituents in marine bacterial ecology. However, it remains unclear how rhodopsins are used in most microorganisms. Despite their abundance in marine and fresh-water systems, the presence of functional rhodopsin systems in edaphic habitats has never been reported. Here, we show the presence of several new putative H+, Na+ and Cl+ pumping rhodopsins identified by metagenomic analysis of Antarctic desert hypolithic communities. Reconstruction of two Proteobacteria genomes harboring xanthorhodopsin-like proteins and one Bacteroidetes genome with a Na-pumping-like rhodopsin indicated that these bacteria were aerobic heterotrophs possessing the apparent capacity for the functional expression of rhodopsins. The existence of these protein systems in hypolithic bacteria expands the known role of rhodopsins to include terrestrial environments and suggests a possible predominant function as heterotrophic energy supply proteins, a feasible microbial adaptation to the harsh conditions prevalent in Antarctic edaphic systems.
Fil: Guerrero, Leandro Demián. Consejo Nacional de Investigaciones Científicas y Técnicas. Instituto de Investigaciones en Ingeniería Genética y Biología Molecular "Dr. Héctor N. Torres"; Argentina. University of Pretoria. Department of Genetics. Centre of Microbial Ecology and Genomics; Sudáfrica
Fil: Vikram, Surendra. University of Pretoria. Department of Genetics. Centre of Microbial Ecology and Genomics; Sudáfrica
Fil: Makhalanyane, Thulani P.. University of Pretoria. Department of Genetics. Centre of Microbial Ecology and Genomics; Sudáfrica
Fil: Cowan, Don A.. University of Pretoria. Department of Genetics. Centre of Microbial Ecology and Genomics; Sudáfrica
description Microorganisms able to synthesize rhodopsins have the capacity to translocate ions through their membranes, using solar energy to generate a proton motive force. Rhodopsins are the most abundant phototrophic proteins in oceanic surface waters and are key constituents in marine bacterial ecology. However, it remains unclear how rhodopsins are used in most microorganisms. Despite their abundance in marine and fresh-water systems, the presence of functional rhodopsin systems in edaphic habitats has never been reported. Here, we show the presence of several new putative H+, Na+ and Cl+ pumping rhodopsins identified by metagenomic analysis of Antarctic desert hypolithic communities. Reconstruction of two Proteobacteria genomes harboring xanthorhodopsin-like proteins and one Bacteroidetes genome with a Na-pumping-like rhodopsin indicated that these bacteria were aerobic heterotrophs possessing the apparent capacity for the functional expression of rhodopsins. The existence of these protein systems in hypolithic bacteria expands the known role of rhodopsins to include terrestrial environments and suggests a possible predominant function as heterotrophic energy supply proteins, a feasible microbial adaptation to the harsh conditions prevalent in Antarctic edaphic systems.
publishDate 2017
dc.date.none.fl_str_mv 2017-09
dc.type.none.fl_str_mv info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
http://purl.org/coar/resource_type/c_6501
info:ar-repo/semantics/articulo
format article
status_str publishedVersion
dc.identifier.none.fl_str_mv http://hdl.handle.net/11336/72604
Guerrero, Leandro Demián; Vikram, Surendra; Makhalanyane, Thulani P.; Cowan, Don A.; Evidence of microbial rhodopsins in Antarctic Dry Valley edaphic systems; Wiley Blackwell Publishing, Inc; Environmental Microbiology; 19; 9; 9-2017; 3755-3767
1462-2912
CONICET Digital
CONICET
url http://hdl.handle.net/11336/72604
identifier_str_mv Guerrero, Leandro Demián; Vikram, Surendra; Makhalanyane, Thulani P.; Cowan, Don A.; Evidence of microbial rhodopsins in Antarctic Dry Valley edaphic systems; Wiley Blackwell Publishing, Inc; Environmental Microbiology; 19; 9; 9-2017; 3755-3767
1462-2912
CONICET Digital
CONICET
dc.language.none.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv info:eu-repo/semantics/altIdentifier/url/https://onlinelibrary.wiley.com/doi/abs/10.1111/1462-2920.13877
info:eu-repo/semantics/altIdentifier/doi/10.1111/1462-2920.13877
dc.rights.none.fl_str_mv info:eu-repo/semantics/openAccess
https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
eu_rights_str_mv openAccess
rights_invalid_str_mv https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
dc.format.none.fl_str_mv application/pdf
application/pdf
dc.publisher.none.fl_str_mv Wiley Blackwell Publishing, Inc
publisher.none.fl_str_mv Wiley Blackwell Publishing, Inc
dc.source.none.fl_str_mv reponame:CONICET Digital (CONICET)
instname:Consejo Nacional de Investigaciones Científicas y Técnicas
reponame_str CONICET Digital (CONICET)
collection CONICET Digital (CONICET)
instname_str Consejo Nacional de Investigaciones Científicas y Técnicas
repository.name.fl_str_mv CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicas
repository.mail.fl_str_mv dasensio@conicet.gov.ar; lcarlino@conicet.gov.ar
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score 13.070432

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