Unraveling the Molecular Basis of Mycosporine Biosynthesis in Fungi
- Autores
- Sepúlveda, Dionisia; Campusano, Sebastián; Moline, Martin; Barahona, Salvador; Baeza, Marcelo; Alcaíno, Jennifer; Colabella, Fernando; Urzúa, Blanca; Libkind Frati, Diego; Cifuentes, Víctor
- Año de publicación
- 2023
- Idioma
- inglés
- Tipo de recurso
- artículo
- Estado
- versión publicada
- Descripción
- The Phaffia rhodozyma UCD 67-385 genome harbors a 7873 bp cluster containing DDGS, OMT, and ATPG, encoding 2-desmethy-4-deoxygadusol synthase, O-methyl transferase, and ATPgrasp ligase, respectively, of the mycosporine glutaminol (MG) biosynthesis pathway. Homozygous deletion mutants of the entire cluster, single-gene mutants, and the ∆ddgs−/−;∆omt−/− and ∆omt−/−;∆atpg−/− double-gene mutants did not produce mycosporines. However, ∆atpg−/− accumulated the intermediate 4-deoxygadusol. Heterologous expression of the DDGS and OMT or DDGS, OMT, and ATPG cDNAs in Saccharomyces cerevisiae led to 4-deoxygadusol or MG production, respectively. Genetic integration of the complete cluster into the genome of the non-mycosporine-producing CBS 6938 wild-type strain resulted in a transgenic strain (CBS 6938_MYC) that produced MG and mycosporine glutaminol glucoside. These results indicate the function of DDGS, OMT, and ATPG in the mycosporine biosynthesis pathway. The transcription factor gene mutants ∆mig1−/−, ∆cyc8−/−, and ∆opi1−/− showed upregulation, ∆rox1−/− and ∆skn7−/− showed downregulation, and ∆tup6−/− and ∆yap6−/− showed no effect on mycosporinogenesis in glucose-containing medium. Finally, comparative analysis of the cluster sequences in several P. rhodozyma strains and the four newly described species of the genus showed the phylogenetic relationship of the P. rhodozyma strains and their differentiation from the other species of the genus Phaffia.
Fil: Sepúlveda, Dionisia. Universidad de Chile; Chile
Fil: Campusano, Sebastián. Universidad de Chile; Chile
Fil: Moline, Martin. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Patagonia Norte. Instituto Andino Patagónico de Tecnologías Biológicas y Geoambientales. Universidad Nacional del Comahue. Instituto Andino Patagónico de Tecnologías Biológicas y Geoambientales; Argentina
Fil: Barahona, Salvador. Universidad de Chile; Chile
Fil: Baeza, Marcelo. Universidad de Chile; Chile
Fil: Alcaíno, Jennifer. Universidad de Chile; Chile
Fil: Colabella, Fernando. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina
Fil: Urzúa, Blanca. Universidad de Chile; Chile
Fil: Libkind Frati, Diego. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Patagonia Norte. Instituto Andino Patagónico de Tecnologías Biológicas y Geoambientales. Universidad Nacional del Comahue. Instituto Andino Patagónico de Tecnologías Biológicas y Geoambientales; Argentina
Fil: Cifuentes, Víctor. Universidad de Chile; Chile - Materia
-
PHAFFIA
GENE CLUSTER
MYCOSPORINE
SECONDARY METABOLITE - Nivel de accesibilidad
- acceso abierto
- Condiciones de uso
- https://creativecommons.org/licenses/by/2.5/ar/
- Repositorio
.jpg)
- Institución
- Consejo Nacional de Investigaciones Científicas y Técnicas
- OAI Identificador
- oai:ri.conicet.gov.ar:11336/235559
Ver los metadatos del registro completo
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Unraveling the Molecular Basis of Mycosporine Biosynthesis in FungiSepúlveda, DionisiaCampusano, SebastiánMoline, MartinBarahona, SalvadorBaeza, MarceloAlcaíno, JenniferColabella, FernandoUrzúa, BlancaLibkind Frati, DiegoCifuentes, VíctorPHAFFIAGENE CLUSTERMYCOSPORINESECONDARY METABOLITEhttps://purl.org/becyt/ford/1.6https://purl.org/becyt/ford/1The Phaffia rhodozyma UCD 67-385 genome harbors a 7873 bp cluster containing DDGS, OMT, and ATPG, encoding 2-desmethy-4-deoxygadusol synthase, O-methyl transferase, and ATPgrasp ligase, respectively, of the mycosporine glutaminol (MG) biosynthesis pathway. Homozygous deletion mutants of the entire cluster, single-gene mutants, and the ∆ddgs−/−;∆omt−/− and ∆omt−/−;∆atpg−/− double-gene mutants did not produce mycosporines. However, ∆atpg−/− accumulated the intermediate 4-deoxygadusol. Heterologous expression of the DDGS and OMT or DDGS, OMT, and ATPG cDNAs in Saccharomyces cerevisiae led to 4-deoxygadusol or MG production, respectively. Genetic integration of the complete cluster into the genome of the non-mycosporine-producing CBS 6938 wild-type strain resulted in a transgenic strain (CBS 6938_MYC) that produced MG and mycosporine glutaminol glucoside. These results indicate the function of DDGS, OMT, and ATPG in the mycosporine biosynthesis pathway. The transcription factor gene mutants ∆mig1−/−, ∆cyc8−/−, and ∆opi1−/− showed upregulation, ∆rox1−/− and ∆skn7−/− showed downregulation, and ∆tup6−/− and ∆yap6−/− showed no effect on mycosporinogenesis in glucose-containing medium. Finally, comparative analysis of the cluster sequences in several P. rhodozyma strains and the four newly described species of the genus showed the phylogenetic relationship of the P. rhodozyma strains and their differentiation from the other species of the genus Phaffia.Fil: Sepúlveda, Dionisia. Universidad de Chile; ChileFil: Campusano, Sebastián. Universidad de Chile; ChileFil: Moline, Martin. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Patagonia Norte. Instituto Andino Patagónico de Tecnologías Biológicas y Geoambientales. Universidad Nacional del Comahue. Instituto Andino Patagónico de Tecnologías Biológicas y Geoambientales; ArgentinaFil: Barahona, Salvador. Universidad de Chile; ChileFil: Baeza, Marcelo. Universidad de Chile; ChileFil: Alcaíno, Jennifer. Universidad de Chile; ChileFil: Colabella, Fernando. Consejo Nacional de Investigaciones Científicas y Técnicas; ArgentinaFil: Urzúa, Blanca. Universidad de Chile; ChileFil: Libkind Frati, Diego. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Patagonia Norte. Instituto Andino Patagónico de Tecnologías Biológicas y Geoambientales. Universidad Nacional del Comahue. Instituto Andino Patagónico de Tecnologías Biológicas y Geoambientales; ArgentinaFil: Cifuentes, Víctor. Universidad de Chile; ChileMolecular Diversity Preservation International2023-03info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionhttp://purl.org/coar/resource_type/c_6501info:ar-repo/semantics/articuloapplication/pdfapplication/pdfapplication/pdfhttp://hdl.handle.net/11336/235559Sepúlveda, Dionisia; Campusano, Sebastián; Moline, Martin; Barahona, Salvador; Baeza, Marcelo; et al.; Unraveling the Molecular Basis of Mycosporine Biosynthesis in Fungi; Molecular Diversity Preservation International; International Journal of Molecular Sciences; 24; 6; 3-2023; 1-191422-0067CONICET DigitalCONICETenginfo:eu-repo/semantics/altIdentifier/url/https://www.mdpi.com/1422-0067/24/6/5930info:eu-repo/semantics/altIdentifier/doi/10.3390/ijms24065930info:eu-repo/semantics/openAccesshttps://creativecommons.org/licenses/by/2.5/ar/reponame:CONICET Digital (CONICET)instname:Consejo Nacional de Investigaciones Científicas y Técnicas2025-09-03T09:43:49Zoai:ri.conicet.gov.ar:11336/235559instacron:CONICETInstitucionalhttp://ri.conicet.gov.ar/Organismo científico-tecnológicoNo correspondehttp://ri.conicet.gov.ar/oai/requestdasensio@conicet.gov.ar; lcarlino@conicet.gov.arArgentinaNo correspondeNo correspondeNo correspondeopendoar:34982025-09-03 09:43:49.427CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicasfalse |
| dc.title.none.fl_str_mv |
Unraveling the Molecular Basis of Mycosporine Biosynthesis in Fungi |
| title |
Unraveling the Molecular Basis of Mycosporine Biosynthesis in Fungi |
| spellingShingle |
Unraveling the Molecular Basis of Mycosporine Biosynthesis in Fungi Sepúlveda, Dionisia PHAFFIA GENE CLUSTER MYCOSPORINE SECONDARY METABOLITE |
| title_short |
Unraveling the Molecular Basis of Mycosporine Biosynthesis in Fungi |
| title_full |
Unraveling the Molecular Basis of Mycosporine Biosynthesis in Fungi |
| title_fullStr |
Unraveling the Molecular Basis of Mycosporine Biosynthesis in Fungi |
| title_full_unstemmed |
Unraveling the Molecular Basis of Mycosporine Biosynthesis in Fungi |
| title_sort |
Unraveling the Molecular Basis of Mycosporine Biosynthesis in Fungi |
| dc.creator.none.fl_str_mv |
Sepúlveda, Dionisia Campusano, Sebastián Moline, Martin Barahona, Salvador Baeza, Marcelo Alcaíno, Jennifer Colabella, Fernando Urzúa, Blanca Libkind Frati, Diego Cifuentes, Víctor |
| author |
Sepúlveda, Dionisia |
| author_facet |
Sepúlveda, Dionisia Campusano, Sebastián Moline, Martin Barahona, Salvador Baeza, Marcelo Alcaíno, Jennifer Colabella, Fernando Urzúa, Blanca Libkind Frati, Diego Cifuentes, Víctor |
| author_role |
author |
| author2 |
Campusano, Sebastián Moline, Martin Barahona, Salvador Baeza, Marcelo Alcaíno, Jennifer Colabella, Fernando Urzúa, Blanca Libkind Frati, Diego Cifuentes, Víctor |
| author2_role |
author author author author author author author author author |
| dc.subject.none.fl_str_mv |
PHAFFIA GENE CLUSTER MYCOSPORINE SECONDARY METABOLITE |
| topic |
PHAFFIA GENE CLUSTER MYCOSPORINE SECONDARY METABOLITE |
| purl_subject.fl_str_mv |
https://purl.org/becyt/ford/1.6 https://purl.org/becyt/ford/1 |
| dc.description.none.fl_txt_mv |
The Phaffia rhodozyma UCD 67-385 genome harbors a 7873 bp cluster containing DDGS, OMT, and ATPG, encoding 2-desmethy-4-deoxygadusol synthase, O-methyl transferase, and ATPgrasp ligase, respectively, of the mycosporine glutaminol (MG) biosynthesis pathway. Homozygous deletion mutants of the entire cluster, single-gene mutants, and the ∆ddgs−/−;∆omt−/− and ∆omt−/−;∆atpg−/− double-gene mutants did not produce mycosporines. However, ∆atpg−/− accumulated the intermediate 4-deoxygadusol. Heterologous expression of the DDGS and OMT or DDGS, OMT, and ATPG cDNAs in Saccharomyces cerevisiae led to 4-deoxygadusol or MG production, respectively. Genetic integration of the complete cluster into the genome of the non-mycosporine-producing CBS 6938 wild-type strain resulted in a transgenic strain (CBS 6938_MYC) that produced MG and mycosporine glutaminol glucoside. These results indicate the function of DDGS, OMT, and ATPG in the mycosporine biosynthesis pathway. The transcription factor gene mutants ∆mig1−/−, ∆cyc8−/−, and ∆opi1−/− showed upregulation, ∆rox1−/− and ∆skn7−/− showed downregulation, and ∆tup6−/− and ∆yap6−/− showed no effect on mycosporinogenesis in glucose-containing medium. Finally, comparative analysis of the cluster sequences in several P. rhodozyma strains and the four newly described species of the genus showed the phylogenetic relationship of the P. rhodozyma strains and their differentiation from the other species of the genus Phaffia. Fil: Sepúlveda, Dionisia. Universidad de Chile; Chile Fil: Campusano, Sebastián. Universidad de Chile; Chile Fil: Moline, Martin. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Patagonia Norte. Instituto Andino Patagónico de Tecnologías Biológicas y Geoambientales. Universidad Nacional del Comahue. Instituto Andino Patagónico de Tecnologías Biológicas y Geoambientales; Argentina Fil: Barahona, Salvador. Universidad de Chile; Chile Fil: Baeza, Marcelo. Universidad de Chile; Chile Fil: Alcaíno, Jennifer. Universidad de Chile; Chile Fil: Colabella, Fernando. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina Fil: Urzúa, Blanca. Universidad de Chile; Chile Fil: Libkind Frati, Diego. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Patagonia Norte. Instituto Andino Patagónico de Tecnologías Biológicas y Geoambientales. Universidad Nacional del Comahue. Instituto Andino Patagónico de Tecnologías Biológicas y Geoambientales; Argentina Fil: Cifuentes, Víctor. Universidad de Chile; Chile |
| description |
The Phaffia rhodozyma UCD 67-385 genome harbors a 7873 bp cluster containing DDGS, OMT, and ATPG, encoding 2-desmethy-4-deoxygadusol synthase, O-methyl transferase, and ATPgrasp ligase, respectively, of the mycosporine glutaminol (MG) biosynthesis pathway. Homozygous deletion mutants of the entire cluster, single-gene mutants, and the ∆ddgs−/−;∆omt−/− and ∆omt−/−;∆atpg−/− double-gene mutants did not produce mycosporines. However, ∆atpg−/− accumulated the intermediate 4-deoxygadusol. Heterologous expression of the DDGS and OMT or DDGS, OMT, and ATPG cDNAs in Saccharomyces cerevisiae led to 4-deoxygadusol or MG production, respectively. Genetic integration of the complete cluster into the genome of the non-mycosporine-producing CBS 6938 wild-type strain resulted in a transgenic strain (CBS 6938_MYC) that produced MG and mycosporine glutaminol glucoside. These results indicate the function of DDGS, OMT, and ATPG in the mycosporine biosynthesis pathway. The transcription factor gene mutants ∆mig1−/−, ∆cyc8−/−, and ∆opi1−/− showed upregulation, ∆rox1−/− and ∆skn7−/− showed downregulation, and ∆tup6−/− and ∆yap6−/− showed no effect on mycosporinogenesis in glucose-containing medium. Finally, comparative analysis of the cluster sequences in several P. rhodozyma strains and the four newly described species of the genus showed the phylogenetic relationship of the P. rhodozyma strains and their differentiation from the other species of the genus Phaffia. |
| publishDate |
2023 |
| dc.date.none.fl_str_mv |
2023-03 |
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info:eu-repo/semantics/article info:eu-repo/semantics/publishedVersion http://purl.org/coar/resource_type/c_6501 info:ar-repo/semantics/articulo |
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article |
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publishedVersion |
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http://hdl.handle.net/11336/235559 Sepúlveda, Dionisia; Campusano, Sebastián; Moline, Martin; Barahona, Salvador; Baeza, Marcelo; et al.; Unraveling the Molecular Basis of Mycosporine Biosynthesis in Fungi; Molecular Diversity Preservation International; International Journal of Molecular Sciences; 24; 6; 3-2023; 1-19 1422-0067 CONICET Digital CONICET |
| url |
http://hdl.handle.net/11336/235559 |
| identifier_str_mv |
Sepúlveda, Dionisia; Campusano, Sebastián; Moline, Martin; Barahona, Salvador; Baeza, Marcelo; et al.; Unraveling the Molecular Basis of Mycosporine Biosynthesis in Fungi; Molecular Diversity Preservation International; International Journal of Molecular Sciences; 24; 6; 3-2023; 1-19 1422-0067 CONICET Digital CONICET |
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eng |
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eng |
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Molecular Diversity Preservation International |
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Molecular Diversity Preservation International |
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CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicas |
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dasensio@conicet.gov.ar; lcarlino@conicet.gov.ar |
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