Peptide Anchor for Folate-Targeted Liposomal Delivery

Autores
Nogueira, Eugénia; Mangialavori, Irene Cecilia; Loureiro, Ana; Azoia, Nuno G.; Sárria, Marisa P.; Nogueira, Patrícia; Freitas, Jaime; Härmark, Johan; Shimanovich, Ulyana; Rollett, Alexandra; Lacroix, Ghislaine; Bernardes, Gonçalo J.L.; Guebitz, Georg; Hebert, Hans; Moreira, Alexandra; Carmo, Alexandre M.; Rossi, Juan Pablo Francisco; Gomes, Andreia C.; Preto, Ana; Cavaco Paulo, Artur
Año de publicación
2015
Idioma
inglés
Tipo de recurso
artículo
Estado
versión publicada
Descripción
Specific folate receptors are abundantly overexpressed in chronically activated macrophages and in most cancer cells. Directed folate receptor targeting using liposomes is usually achieved using folate linked to a phospholipid or cholesterol anchor. This link is formed using a large spacer like polyethylene glycol. Here, we report an innovative strategy for targeted liposome delivery that uses a hydrophobic fragment of surfactant protein D linked to folate. Our proposed spacer is a small 4 amino acid residue linker. The peptide conjugate inserts deeply into the lipid bilayer without affecting liposomal integrity, with high stability and specificity. To compare the drug delivery potential of both liposomal targeting systems, we encapsulated the nuclear dye Hoechst 34580. The eventual increase in blue fluorescence would only be detectable upon liposome disruption, leading to specific binding of this dye to DNA. Our delivery system was proven to be more efficient (2-fold) in Caco-2 cells than classic systems where the folate moiety is linked to liposomes by polyethylene glycol.
Fil: Nogueira, Eugénia. Universidade do Minho; Portugal
Fil: Mangialavori, Irene Cecilia. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Houssay. Instituto de Química y Físico-Química Biológicas "Prof. Alejandro C. Paladini". Universidad de Buenos Aires. Facultad de Farmacia y Bioquímica. Instituto de Química y Físico-Química Biológicas; Argentina
Fil: Loureiro, Ana. Universidade do Minho; Portugal
Fil: Azoia, Nuno G.. Universidade do Minho; Portugal
Fil: Sárria, Marisa P.. Universidade do Minho; Portugal
Fil: Nogueira, Patrícia. Universidad de Porto; Portugal. Instituto de Biologia Molecular e Celular; Brasil
Fil: Freitas, Jaime. Instituto de Biologia Molecular e Celular; Brasil. Universidad de Porto; Portugal
Fil: Härmark, Johan. Karolinska Huddinge Hospital. Karolinska Institutet; Suecia
Fil: Shimanovich, Ulyana. University of Cambridge; Estados Unidos
Fil: Rollett, Alexandra. University of Natural Resources and Life Sciences; Austria
Fil: Lacroix, Ghislaine. Institut National de l’Environnement Industriel et des Risques; Francia
Fil: Bernardes, Gonçalo J.L.. University of Cambridge; Estados Unidos
Fil: Guebitz, Georg. University of Natural Resources and Life Sciences; Austria
Fil: Hebert, Hans. Karolinska Huddinge Hospital. Karolinska Institutet; Suecia
Fil: Moreira, Alexandra. Instituto de Biologia Molecular e Celular; Portugal. Universidad de Porto; Portugal
Fil: Carmo, Alexandre M.. Instituto de Biologia Molecular e Celular; Portugal. Universidad de Porto; Portugal
Fil: Rossi, Juan Pablo Francisco. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Houssay. Instituto de Química y Físico-Química Biológicas "Prof. Alejandro C. Paladini". Universidad de Buenos Aires. Facultad de Farmacia y Bioquímica. Instituto de Química y Físico-Química Biológicas; Argentina
Fil: Gomes, Andreia C.. Universidade do Minho; Portugal
Fil: Preto, Ana. Universidade do Minho; Portugal
Fil: Cavaco Paulo, Artur. Universidade do Minho; Portugal
Materia
FOLATE RECEPTORS
PEPTIDE CONJUGATE
NANOLIPOSOMES
DROG DELIVERY
Nivel de accesibilidad
acceso abierto
Condiciones de uso
https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
Repositorio
CONICET Digital (CONICET)
Institución
Consejo Nacional de Investigaciones Científicas y Técnicas
OAI Identificador
oai:ri.conicet.gov.ar:11336/100056
Acceder
id CONICETDig_205143c8569fa883d067ad5a4ae9c5f7
oai_identifier_str oai:ri.conicet.gov.ar:11336/100056
network_acronym_str CONICETDig
repository_id_str 3498
network_name_str CONICET Digital (CONICET)
spelling Peptide Anchor for Folate-Targeted Liposomal DeliveryNogueira, EugéniaMangialavori, Irene CeciliaLoureiro, AnaAzoia, Nuno G.Sárria, Marisa P.Nogueira, PatríciaFreitas, JaimeHärmark, JohanShimanovich, UlyanaRollett, AlexandraLacroix, GhislaineBernardes, Gonçalo J.L.Guebitz, GeorgHebert, HansMoreira, AlexandraCarmo, Alexandre M.Rossi, Juan Pablo FranciscoGomes, Andreia C.Preto, AnaCavaco Paulo, ArturFOLATE RECEPTORSPEPTIDE CONJUGATENANOLIPOSOMESDROG DELIVERYhttps://purl.org/becyt/ford/2.10https://purl.org/becyt/ford/2Specific folate receptors are abundantly overexpressed in chronically activated macrophages and in most cancer cells. Directed folate receptor targeting using liposomes is usually achieved using folate linked to a phospholipid or cholesterol anchor. This link is formed using a large spacer like polyethylene glycol. Here, we report an innovative strategy for targeted liposome delivery that uses a hydrophobic fragment of surfactant protein D linked to folate. Our proposed spacer is a small 4 amino acid residue linker. The peptide conjugate inserts deeply into the lipid bilayer without affecting liposomal integrity, with high stability and specificity. To compare the drug delivery potential of both liposomal targeting systems, we encapsulated the nuclear dye Hoechst 34580. The eventual increase in blue fluorescence would only be detectable upon liposome disruption, leading to specific binding of this dye to DNA. Our delivery system was proven to be more efficient (2-fold) in Caco-2 cells than classic systems where the folate moiety is linked to liposomes by polyethylene glycol.Fil: Nogueira, Eugénia. Universidade do Minho; PortugalFil: Mangialavori, Irene Cecilia. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Houssay. Instituto de Química y Físico-Química Biológicas "Prof. Alejandro C. Paladini". Universidad de Buenos Aires. Facultad de Farmacia y Bioquímica. Instituto de Química y Físico-Química Biológicas; ArgentinaFil: Loureiro, Ana. Universidade do Minho; PortugalFil: Azoia, Nuno G.. Universidade do Minho; PortugalFil: Sárria, Marisa P.. Universidade do Minho; PortugalFil: Nogueira, Patrícia. Universidad de Porto; Portugal. Instituto de Biologia Molecular e Celular; BrasilFil: Freitas, Jaime. Instituto de Biologia Molecular e Celular; Brasil. Universidad de Porto; PortugalFil: Härmark, Johan. Karolinska Huddinge Hospital. Karolinska Institutet; SueciaFil: Shimanovich, Ulyana. University of Cambridge; Estados UnidosFil: Rollett, Alexandra. University of Natural Resources and Life Sciences; AustriaFil: Lacroix, Ghislaine. Institut National de l’Environnement Industriel et des Risques; FranciaFil: Bernardes, Gonçalo J.L.. University of Cambridge; Estados UnidosFil: Guebitz, Georg. University of Natural Resources and Life Sciences; AustriaFil: Hebert, Hans. Karolinska Huddinge Hospital. Karolinska Institutet; SueciaFil: Moreira, Alexandra. Instituto de Biologia Molecular e Celular; Portugal. Universidad de Porto; PortugalFil: Carmo, Alexandre M.. Instituto de Biologia Molecular e Celular; Portugal. Universidad de Porto; PortugalFil: Rossi, Juan Pablo Francisco. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Houssay. Instituto de Química y Físico-Química Biológicas "Prof. Alejandro C. Paladini". Universidad de Buenos Aires. Facultad de Farmacia y Bioquímica. Instituto de Química y Físico-Química Biológicas; ArgentinaFil: Gomes, Andreia C.. Universidade do Minho; PortugalFil: Preto, Ana. Universidade do Minho; PortugalFil: Cavaco Paulo, Artur. Universidade do Minho; PortugalAmerican Chemical Society2015-09info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionhttp://purl.org/coar/resource_type/c_6501info:ar-repo/semantics/articuloapplication/pdfapplication/pdfapplication/pdfhttp://hdl.handle.net/11336/100056Nogueira, Eugénia; Mangialavori, Irene Cecilia; Loureiro, Ana; Azoia, Nuno G.; Sárria, Marisa P.; et al.; Peptide Anchor for Folate-Targeted Liposomal Delivery; American Chemical Society; Biomacromolecules; 16; 9; 9-2015; 2904-29101525-7797CONICET DigitalCONICETenginfo:eu-repo/semantics/altIdentifier/url/https://pubs.acs.org/doi/abs/10.1021/acs.biomac.5b00823info:eu-repo/semantics/altIdentifier/doi/10.1021/acs.biomac.5b00823info:eu-repo/semantics/openAccesshttps://creativecommons.org/licenses/by-nc-sa/2.5/ar/reponame:CONICET Digital (CONICET)instname:Consejo Nacional de Investigaciones Científicas y Técnicas2025-10-15T14:40:39Zoai:ri.conicet.gov.ar:11336/100056instacron:CONICETInstitucionalhttp://ri.conicet.gov.ar/Organismo científico-tecnológicoNo correspondehttp://ri.conicet.gov.ar/oai/requestdasensio@conicet.gov.ar; lcarlino@conicet.gov.arArgentinaNo correspondeNo correspondeNo correspondeopendoar:34982025-10-15 14:40:39.485CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicasfalse
dc.title.none.fl_str_mv Peptide Anchor for Folate-Targeted Liposomal Delivery
title Peptide Anchor for Folate-Targeted Liposomal Delivery
spellingShingle Peptide Anchor for Folate-Targeted Liposomal Delivery
Nogueira, Eugénia
FOLATE RECEPTORS
PEPTIDE CONJUGATE
NANOLIPOSOMES
DROG DELIVERY
title_short Peptide Anchor for Folate-Targeted Liposomal Delivery
title_full Peptide Anchor for Folate-Targeted Liposomal Delivery
title_fullStr Peptide Anchor for Folate-Targeted Liposomal Delivery
title_full_unstemmed Peptide Anchor for Folate-Targeted Liposomal Delivery
title_sort Peptide Anchor for Folate-Targeted Liposomal Delivery
dc.creator.none.fl_str_mv Nogueira, Eugénia
Mangialavori, Irene Cecilia
Loureiro, Ana
Azoia, Nuno G.
Sárria, Marisa P.
Nogueira, Patrícia
Freitas, Jaime
Härmark, Johan
Shimanovich, Ulyana
Rollett, Alexandra
Lacroix, Ghislaine
Bernardes, Gonçalo J.L.
Guebitz, Georg
Hebert, Hans
Moreira, Alexandra
Carmo, Alexandre M.
Rossi, Juan Pablo Francisco
Gomes, Andreia C.
Preto, Ana
Cavaco Paulo, Artur
author Nogueira, Eugénia
author_facet Nogueira, Eugénia
Mangialavori, Irene Cecilia
Loureiro, Ana
Azoia, Nuno G.
Sárria, Marisa P.
Nogueira, Patrícia
Freitas, Jaime
Härmark, Johan
Shimanovich, Ulyana
Rollett, Alexandra
Lacroix, Ghislaine
Bernardes, Gonçalo J.L.
Guebitz, Georg
Hebert, Hans
Moreira, Alexandra
Carmo, Alexandre M.
Rossi, Juan Pablo Francisco
Gomes, Andreia C.
Preto, Ana
Cavaco Paulo, Artur
author_role author
author2 Mangialavori, Irene Cecilia
Loureiro, Ana
Azoia, Nuno G.
Sárria, Marisa P.
Nogueira, Patrícia
Freitas, Jaime
Härmark, Johan
Shimanovich, Ulyana
Rollett, Alexandra
Lacroix, Ghislaine
Bernardes, Gonçalo J.L.
Guebitz, Georg
Hebert, Hans
Moreira, Alexandra
Carmo, Alexandre M.
Rossi, Juan Pablo Francisco
Gomes, Andreia C.
Preto, Ana
Cavaco Paulo, Artur
author2_role author
author
author
author
author
author
author
author
author
author
author
author
author
author
author
author
author
author
author
dc.subject.none.fl_str_mv FOLATE RECEPTORS
PEPTIDE CONJUGATE
NANOLIPOSOMES
DROG DELIVERY
topic FOLATE RECEPTORS
PEPTIDE CONJUGATE
NANOLIPOSOMES
DROG DELIVERY
purl_subject.fl_str_mv https://purl.org/becyt/ford/2.10
https://purl.org/becyt/ford/2
dc.description.none.fl_txt_mv Specific folate receptors are abundantly overexpressed in chronically activated macrophages and in most cancer cells. Directed folate receptor targeting using liposomes is usually achieved using folate linked to a phospholipid or cholesterol anchor. This link is formed using a large spacer like polyethylene glycol. Here, we report an innovative strategy for targeted liposome delivery that uses a hydrophobic fragment of surfactant protein D linked to folate. Our proposed spacer is a small 4 amino acid residue linker. The peptide conjugate inserts deeply into the lipid bilayer without affecting liposomal integrity, with high stability and specificity. To compare the drug delivery potential of both liposomal targeting systems, we encapsulated the nuclear dye Hoechst 34580. The eventual increase in blue fluorescence would only be detectable upon liposome disruption, leading to specific binding of this dye to DNA. Our delivery system was proven to be more efficient (2-fold) in Caco-2 cells than classic systems where the folate moiety is linked to liposomes by polyethylene glycol.
Fil: Nogueira, Eugénia. Universidade do Minho; Portugal
Fil: Mangialavori, Irene Cecilia. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Houssay. Instituto de Química y Físico-Química Biológicas "Prof. Alejandro C. Paladini". Universidad de Buenos Aires. Facultad de Farmacia y Bioquímica. Instituto de Química y Físico-Química Biológicas; Argentina
Fil: Loureiro, Ana. Universidade do Minho; Portugal
Fil: Azoia, Nuno G.. Universidade do Minho; Portugal
Fil: Sárria, Marisa P.. Universidade do Minho; Portugal
Fil: Nogueira, Patrícia. Universidad de Porto; Portugal. Instituto de Biologia Molecular e Celular; Brasil
Fil: Freitas, Jaime. Instituto de Biologia Molecular e Celular; Brasil. Universidad de Porto; Portugal
Fil: Härmark, Johan. Karolinska Huddinge Hospital. Karolinska Institutet; Suecia
Fil: Shimanovich, Ulyana. University of Cambridge; Estados Unidos
Fil: Rollett, Alexandra. University of Natural Resources and Life Sciences; Austria
Fil: Lacroix, Ghislaine. Institut National de l’Environnement Industriel et des Risques; Francia
Fil: Bernardes, Gonçalo J.L.. University of Cambridge; Estados Unidos
Fil: Guebitz, Georg. University of Natural Resources and Life Sciences; Austria
Fil: Hebert, Hans. Karolinska Huddinge Hospital. Karolinska Institutet; Suecia
Fil: Moreira, Alexandra. Instituto de Biologia Molecular e Celular; Portugal. Universidad de Porto; Portugal
Fil: Carmo, Alexandre M.. Instituto de Biologia Molecular e Celular; Portugal. Universidad de Porto; Portugal
Fil: Rossi, Juan Pablo Francisco. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Houssay. Instituto de Química y Físico-Química Biológicas "Prof. Alejandro C. Paladini". Universidad de Buenos Aires. Facultad de Farmacia y Bioquímica. Instituto de Química y Físico-Química Biológicas; Argentina
Fil: Gomes, Andreia C.. Universidade do Minho; Portugal
Fil: Preto, Ana. Universidade do Minho; Portugal
Fil: Cavaco Paulo, Artur. Universidade do Minho; Portugal
description Specific folate receptors are abundantly overexpressed in chronically activated macrophages and in most cancer cells. Directed folate receptor targeting using liposomes is usually achieved using folate linked to a phospholipid or cholesterol anchor. This link is formed using a large spacer like polyethylene glycol. Here, we report an innovative strategy for targeted liposome delivery that uses a hydrophobic fragment of surfactant protein D linked to folate. Our proposed spacer is a small 4 amino acid residue linker. The peptide conjugate inserts deeply into the lipid bilayer without affecting liposomal integrity, with high stability and specificity. To compare the drug delivery potential of both liposomal targeting systems, we encapsulated the nuclear dye Hoechst 34580. The eventual increase in blue fluorescence would only be detectable upon liposome disruption, leading to specific binding of this dye to DNA. Our delivery system was proven to be more efficient (2-fold) in Caco-2 cells than classic systems where the folate moiety is linked to liposomes by polyethylene glycol.
publishDate 2015
dc.date.none.fl_str_mv 2015-09
dc.type.none.fl_str_mv info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
http://purl.org/coar/resource_type/c_6501
info:ar-repo/semantics/articulo
format article
status_str publishedVersion
dc.identifier.none.fl_str_mv http://hdl.handle.net/11336/100056
Nogueira, Eugénia; Mangialavori, Irene Cecilia; Loureiro, Ana; Azoia, Nuno G.; Sárria, Marisa P.; et al.; Peptide Anchor for Folate-Targeted Liposomal Delivery; American Chemical Society; Biomacromolecules; 16; 9; 9-2015; 2904-2910
1525-7797
CONICET Digital
CONICET
url http://hdl.handle.net/11336/100056
identifier_str_mv Nogueira, Eugénia; Mangialavori, Irene Cecilia; Loureiro, Ana; Azoia, Nuno G.; Sárria, Marisa P.; et al.; Peptide Anchor for Folate-Targeted Liposomal Delivery; American Chemical Society; Biomacromolecules; 16; 9; 9-2015; 2904-2910
1525-7797
CONICET Digital
CONICET
dc.language.none.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv info:eu-repo/semantics/altIdentifier/url/https://pubs.acs.org/doi/abs/10.1021/acs.biomac.5b00823
info:eu-repo/semantics/altIdentifier/doi/10.1021/acs.biomac.5b00823
dc.rights.none.fl_str_mv info:eu-repo/semantics/openAccess
https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
eu_rights_str_mv openAccess
rights_invalid_str_mv https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
dc.format.none.fl_str_mv application/pdf
application/pdf
application/pdf
dc.publisher.none.fl_str_mv American Chemical Society
publisher.none.fl_str_mv American Chemical Society
dc.source.none.fl_str_mv reponame:CONICET Digital (CONICET)
instname:Consejo Nacional de Investigaciones Científicas y Técnicas
reponame_str CONICET Digital (CONICET)
collection CONICET Digital (CONICET)
instname_str Consejo Nacional de Investigaciones Científicas y Técnicas
repository.name.fl_str_mv CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicas
repository.mail.fl_str_mv dasensio@conicet.gov.ar; lcarlino@conicet.gov.ar
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score 13.22299

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