A robust and practically free of charge intermittent use glucose biosensor

Autores
Colombo, Lucas; Garay, Fernando Sebastian
Año de publicación
2017
Idioma
inglés
Tipo de recurso
artículo
Estado
versión publicada
Descripción
In the past decades, great effort has been put in finding new electrode surface modifiers and enzyme immobilizer agents that prevent the enzyme leakage, minimize the effect of interfering species, retain the enzyme bioactivity, and enhance the sensor sensitivity. In this work, a sandwich-type glucose biosensor that keeps its sensitivity and operational linear range for more than a year is presented. After 5 months of intermittent use, where the biosensor was exposed to more than 500 standard additions, it presented a limit of detection of 5 μM, and the linear behavior was from 5 μM to 3 mM with a value of r2 = 0.999. Besides, after 7 months of its assembling, the biosensor was employed for assessing the glucose concentration of real serum samples and its performance was compared with the response of a commercial autoanalyzer. A year later, the biosensor still exhibited very good performance of its analytical parameters. The performance of identical sandwich-type biosensors is analyzed when they are exposed to three different storage conditions. Simulated curves are compared with experimental data to explain the dependence of sensitivity and response-time on the aging and storage conditions of the biosensors.
Fil: Colombo, Lucas. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Córdoba. Instituto de Investigaciones en Físico-química de Córdoba. Universidad Nacional de Córdoba. Facultad de Ciencias Químicas. Instituto de Investigaciones en Físico-química de Córdoba; Argentina
Fil: Garay, Fernando Sebastian. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Córdoba. Instituto de Investigaciones en Físico-química de Córdoba. Universidad Nacional de Córdoba. Facultad de Ciencias Químicas. Instituto de Investigaciones en Físico-química de Córdoba; Argentina
Materia
Biosensor
Chronoamperometry
Diabetes
Glucose
Intermittent Use
Long-Term Stability
Nivel de accesibilidad
acceso abierto
Condiciones de uso
https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
Repositorio
CONICET Digital (CONICET)
Institución
Consejo Nacional de Investigaciones Científicas y Técnicas
OAI Identificador
oai:ri.conicet.gov.ar:11336/64341
Acceder
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oai_identifier_str oai:ri.conicet.gov.ar:11336/64341
network_acronym_str CONICETDig
repository_id_str 3498
network_name_str CONICET Digital (CONICET)
spelling A robust and practically free of charge intermittent use glucose biosensorColombo, LucasGaray, Fernando SebastianBiosensorChronoamperometryDiabetesGlucoseIntermittent UseLong-Term Stabilityhttps://purl.org/becyt/ford/1.4https://purl.org/becyt/ford/1In the past decades, great effort has been put in finding new electrode surface modifiers and enzyme immobilizer agents that prevent the enzyme leakage, minimize the effect of interfering species, retain the enzyme bioactivity, and enhance the sensor sensitivity. In this work, a sandwich-type glucose biosensor that keeps its sensitivity and operational linear range for more than a year is presented. After 5 months of intermittent use, where the biosensor was exposed to more than 500 standard additions, it presented a limit of detection of 5 μM, and the linear behavior was from 5 μM to 3 mM with a value of r2 = 0.999. Besides, after 7 months of its assembling, the biosensor was employed for assessing the glucose concentration of real serum samples and its performance was compared with the response of a commercial autoanalyzer. A year later, the biosensor still exhibited very good performance of its analytical parameters. The performance of identical sandwich-type biosensors is analyzed when they are exposed to three different storage conditions. Simulated curves are compared with experimental data to explain the dependence of sensitivity and response-time on the aging and storage conditions of the biosensors.Fil: Colombo, Lucas. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Córdoba. Instituto de Investigaciones en Físico-química de Córdoba. Universidad Nacional de Córdoba. Facultad de Ciencias Químicas. Instituto de Investigaciones en Físico-química de Córdoba; ArgentinaFil: Garay, Fernando Sebastian. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Córdoba. Instituto de Investigaciones en Físico-química de Córdoba. Universidad Nacional de Córdoba. Facultad de Ciencias Químicas. Instituto de Investigaciones en Físico-química de Córdoba; ArgentinaElsevier Science Sa2017-10info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionhttp://purl.org/coar/resource_type/c_6501info:ar-repo/semantics/articuloapplication/pdfapplication/pdfhttp://hdl.handle.net/11336/64341Colombo, Lucas; Garay, Fernando Sebastian; A robust and practically free of charge intermittent use glucose biosensor; Elsevier Science Sa; Sensors and Actuators B: Chemical; 256; 10-2017; 751-7590925-4005CONICET DigitalCONICETenginfo:eu-repo/semantics/altIdentifier/doi/10.1016/j.snb.2017.10.010info:eu-repo/semantics/altIdentifier/url/https://linkinghub.elsevier.com/retrieve/pii/S0925400517318907info:eu-repo/semantics/openAccesshttps://creativecommons.org/licenses/by-nc-sa/2.5/ar/reponame:CONICET Digital (CONICET)instname:Consejo Nacional de Investigaciones Científicas y Técnicas2025-09-10T13:05:48Zoai:ri.conicet.gov.ar:11336/64341instacron:CONICETInstitucionalhttp://ri.conicet.gov.ar/Organismo científico-tecnológicoNo correspondehttp://ri.conicet.gov.ar/oai/requestdasensio@conicet.gov.ar; lcarlino@conicet.gov.arArgentinaNo correspondeNo correspondeNo correspondeopendoar:34982025-09-10 13:05:49.051CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicasfalse
dc.title.none.fl_str_mv A robust and practically free of charge intermittent use glucose biosensor
title A robust and practically free of charge intermittent use glucose biosensor
spellingShingle A robust and practically free of charge intermittent use glucose biosensor
Colombo, Lucas
Biosensor
Chronoamperometry
Diabetes
Glucose
Intermittent Use
Long-Term Stability
title_short A robust and practically free of charge intermittent use glucose biosensor
title_full A robust and practically free of charge intermittent use glucose biosensor
title_fullStr A robust and practically free of charge intermittent use glucose biosensor
title_full_unstemmed A robust and practically free of charge intermittent use glucose biosensor
title_sort A robust and practically free of charge intermittent use glucose biosensor
dc.creator.none.fl_str_mv Colombo, Lucas
Garay, Fernando Sebastian
author Colombo, Lucas
author_facet Colombo, Lucas
Garay, Fernando Sebastian
author_role author
author2 Garay, Fernando Sebastian
author2_role author
dc.subject.none.fl_str_mv Biosensor
Chronoamperometry
Diabetes
Glucose
Intermittent Use
Long-Term Stability
topic Biosensor
Chronoamperometry
Diabetes
Glucose
Intermittent Use
Long-Term Stability
purl_subject.fl_str_mv https://purl.org/becyt/ford/1.4
https://purl.org/becyt/ford/1
dc.description.none.fl_txt_mv In the past decades, great effort has been put in finding new electrode surface modifiers and enzyme immobilizer agents that prevent the enzyme leakage, minimize the effect of interfering species, retain the enzyme bioactivity, and enhance the sensor sensitivity. In this work, a sandwich-type glucose biosensor that keeps its sensitivity and operational linear range for more than a year is presented. After 5 months of intermittent use, where the biosensor was exposed to more than 500 standard additions, it presented a limit of detection of 5 μM, and the linear behavior was from 5 μM to 3 mM with a value of r2 = 0.999. Besides, after 7 months of its assembling, the biosensor was employed for assessing the glucose concentration of real serum samples and its performance was compared with the response of a commercial autoanalyzer. A year later, the biosensor still exhibited very good performance of its analytical parameters. The performance of identical sandwich-type biosensors is analyzed when they are exposed to three different storage conditions. Simulated curves are compared with experimental data to explain the dependence of sensitivity and response-time on the aging and storage conditions of the biosensors.
Fil: Colombo, Lucas. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Córdoba. Instituto de Investigaciones en Físico-química de Córdoba. Universidad Nacional de Córdoba. Facultad de Ciencias Químicas. Instituto de Investigaciones en Físico-química de Córdoba; Argentina
Fil: Garay, Fernando Sebastian. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Córdoba. Instituto de Investigaciones en Físico-química de Córdoba. Universidad Nacional de Córdoba. Facultad de Ciencias Químicas. Instituto de Investigaciones en Físico-química de Córdoba; Argentina
description In the past decades, great effort has been put in finding new electrode surface modifiers and enzyme immobilizer agents that prevent the enzyme leakage, minimize the effect of interfering species, retain the enzyme bioactivity, and enhance the sensor sensitivity. In this work, a sandwich-type glucose biosensor that keeps its sensitivity and operational linear range for more than a year is presented. After 5 months of intermittent use, where the biosensor was exposed to more than 500 standard additions, it presented a limit of detection of 5 μM, and the linear behavior was from 5 μM to 3 mM with a value of r2 = 0.999. Besides, after 7 months of its assembling, the biosensor was employed for assessing the glucose concentration of real serum samples and its performance was compared with the response of a commercial autoanalyzer. A year later, the biosensor still exhibited very good performance of its analytical parameters. The performance of identical sandwich-type biosensors is analyzed when they are exposed to three different storage conditions. Simulated curves are compared with experimental data to explain the dependence of sensitivity and response-time on the aging and storage conditions of the biosensors.
publishDate 2017
dc.date.none.fl_str_mv 2017-10
dc.type.none.fl_str_mv info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
http://purl.org/coar/resource_type/c_6501
info:ar-repo/semantics/articulo
format article
status_str publishedVersion
dc.identifier.none.fl_str_mv http://hdl.handle.net/11336/64341
Colombo, Lucas; Garay, Fernando Sebastian; A robust and practically free of charge intermittent use glucose biosensor; Elsevier Science Sa; Sensors and Actuators B: Chemical; 256; 10-2017; 751-759
0925-4005
CONICET Digital
CONICET
url http://hdl.handle.net/11336/64341
identifier_str_mv Colombo, Lucas; Garay, Fernando Sebastian; A robust and practically free of charge intermittent use glucose biosensor; Elsevier Science Sa; Sensors and Actuators B: Chemical; 256; 10-2017; 751-759
0925-4005
CONICET Digital
CONICET
dc.language.none.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv info:eu-repo/semantics/altIdentifier/doi/10.1016/j.snb.2017.10.010
info:eu-repo/semantics/altIdentifier/url/https://linkinghub.elsevier.com/retrieve/pii/S0925400517318907
dc.rights.none.fl_str_mv info:eu-repo/semantics/openAccess
https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
eu_rights_str_mv openAccess
rights_invalid_str_mv https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
dc.format.none.fl_str_mv application/pdf
application/pdf
dc.publisher.none.fl_str_mv Elsevier Science Sa
publisher.none.fl_str_mv Elsevier Science Sa
dc.source.none.fl_str_mv reponame:CONICET Digital (CONICET)
instname:Consejo Nacional de Investigaciones Científicas y Técnicas
reponame_str CONICET Digital (CONICET)
collection CONICET Digital (CONICET)
instname_str Consejo Nacional de Investigaciones Científicas y Técnicas
repository.name.fl_str_mv CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicas
repository.mail.fl_str_mv dasensio@conicet.gov.ar; lcarlino@conicet.gov.ar
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score 12.993085

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