Proteomic study of the exponential-stationary growth phase transition in the haloarchaea Natrialba magadii and Haloferax volcanii

Autores
Cerletti, Micaela; Gimenez, Maria Ines; Tröetschel, Christian; D'alessandro, Celeste Paola; Poetsch, Ansgar; de Castro, Rosana Esther; Paggi, Roberto Alejandro
Año de publicación
2018
Idioma
inglés
Tipo de recurso
artículo
Estado
versión publicada
Descripción
The dynamic changes that take place along the phases of microbial growth (lag, exponential, stationary, and death) have been widely studied in bacteria at the molecular and cellular levels, but little is known for archaea. In this study, a high-throughput approach was used to analyze and compare the proteomes of two haloarchaea during exponential and stationary growth: the neutrophilic Haloferax volcanii and the alkaliphilic Natrialba magadii. Almost 2000 proteins were identified in each species (≈50% of the predicted proteome). Among them, 532 and 432 were found to be differential between growth phases in H. volcanii and N. magadii, respectively. Changes upon entrance into stationary phase included an overall increase in proteins involved in the transport of small molecules and ions, stress response, and fatty acid catabolism. Proteins related to genetic processes and cell division showed a notorious decrease in amount. The data reported in this study not only contributes to our understanding of the exponential–stationary growth phase transition in extremophilic archaea but also provides the first comprehensive analysis of the proteome composition of N. magadii. The MS proteomics data have been deposited in the ProteomeXchange Consortium with the dataset identifier JPST000395.
Fil: Cerletti, Micaela. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Mar del Plata. Instituto de Investigaciones Biológicas. Universidad Nacional de Mar del Plata. Facultad de Ciencias Exactas y Naturales. Instituto de Investigaciones Biológicas; Argentina. Universidad Nacional de Mar del Plata; Argentina
Fil: Gimenez, Maria Ines. Universidad Nacional de Mar del Plata; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Mar del Plata. Instituto de Investigaciones Biológicas. Universidad Nacional de Mar del Plata. Facultad de Ciencias Exactas y Naturales. Instituto de Investigaciones Biológicas; Argentina
Fil: Tröetschel, Christian. Ruhr Universität Bochum; Alemania
Fil: D'alessandro, Celeste Paola. Universidade de Sao Paulo; Brasil
Fil: Poetsch, Ansgar. University Of Plymouth; . Ruhr Universität Bochum; Alemania
Fil: de Castro, Rosana Esther. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Mar del Plata. Instituto de Investigaciones Biológicas. Universidad Nacional de Mar del Plata. Facultad de Ciencias Exactas y Naturales. Instituto de Investigaciones Biológicas; Argentina. Universidad Nacional de Mar del Plata; Argentina
Fil: Paggi, Roberto Alejandro. Universidad Nacional de Mar del Plata; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Mar del Plata. Instituto de Investigaciones Biológicas. Universidad Nacional de Mar del Plata. Facultad de Ciencias Exactas y Naturales. Instituto de Investigaciones Biológicas; Argentina
Materia
Comparative proteomics
Haloarchaea
Haloferax volcanii
Natrialba maagadii
Stationary phase
Nivel de accesibilidad
acceso abierto
Condiciones de uso
https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
Repositorio
CONICET Digital (CONICET)
Institución
Consejo Nacional de Investigaciones Científicas y Técnicas
OAI Identificador
oai:ri.conicet.gov.ar:11336/88115
Acceder
id CONICETDig_1fce95623f8a83f124b1c97b103bf325
oai_identifier_str oai:ri.conicet.gov.ar:11336/88115
network_acronym_str CONICETDig
repository_id_str 3498
network_name_str CONICET Digital (CONICET)
spelling Proteomic study of the exponential-stationary growth phase transition in the haloarchaea Natrialba magadii and Haloferax volcaniiCerletti, MicaelaGimenez, Maria InesTröetschel, ChristianD'alessandro, Celeste PaolaPoetsch, Ansgarde Castro, Rosana EstherPaggi, Roberto AlejandroComparative proteomicsHaloarchaeaHaloferax volcaniiNatrialba maagadiiStationary phasehttps://purl.org/becyt/ford/1.6https://purl.org/becyt/ford/1The dynamic changes that take place along the phases of microbial growth (lag, exponential, stationary, and death) have been widely studied in bacteria at the molecular and cellular levels, but little is known for archaea. In this study, a high-throughput approach was used to analyze and compare the proteomes of two haloarchaea during exponential and stationary growth: the neutrophilic Haloferax volcanii and the alkaliphilic Natrialba magadii. Almost 2000 proteins were identified in each species (≈50% of the predicted proteome). Among them, 532 and 432 were found to be differential between growth phases in H. volcanii and N. magadii, respectively. Changes upon entrance into stationary phase included an overall increase in proteins involved in the transport of small molecules and ions, stress response, and fatty acid catabolism. Proteins related to genetic processes and cell division showed a notorious decrease in amount. The data reported in this study not only contributes to our understanding of the exponential–stationary growth phase transition in extremophilic archaea but also provides the first comprehensive analysis of the proteome composition of N. magadii. The MS proteomics data have been deposited in the ProteomeXchange Consortium with the dataset identifier JPST000395.Fil: Cerletti, Micaela. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Mar del Plata. Instituto de Investigaciones Biológicas. Universidad Nacional de Mar del Plata. Facultad de Ciencias Exactas y Naturales. Instituto de Investigaciones Biológicas; Argentina. Universidad Nacional de Mar del Plata; ArgentinaFil: Gimenez, Maria Ines. Universidad Nacional de Mar del Plata; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Mar del Plata. Instituto de Investigaciones Biológicas. Universidad Nacional de Mar del Plata. Facultad de Ciencias Exactas y Naturales. Instituto de Investigaciones Biológicas; ArgentinaFil: Tröetschel, Christian. Ruhr Universität Bochum; AlemaniaFil: D'alessandro, Celeste Paola. Universidade de Sao Paulo; BrasilFil: Poetsch, Ansgar. University Of Plymouth; . Ruhr Universität Bochum; AlemaniaFil: de Castro, Rosana Esther. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Mar del Plata. Instituto de Investigaciones Biológicas. Universidad Nacional de Mar del Plata. Facultad de Ciencias Exactas y Naturales. Instituto de Investigaciones Biológicas; Argentina. Universidad Nacional de Mar del Plata; ArgentinaFil: Paggi, Roberto Alejandro. Universidad Nacional de Mar del Plata; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Mar del Plata. Instituto de Investigaciones Biológicas. Universidad Nacional de Mar del Plata. Facultad de Ciencias Exactas y Naturales. Instituto de Investigaciones Biológicas; ArgentinaWiley VCH Verlag2018-07info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionhttp://purl.org/coar/resource_type/c_6501info:ar-repo/semantics/articuloapplication/pdfapplication/pdfapplication/pdfapplication/pdfapplication/pdfhttp://hdl.handle.net/11336/88115Cerletti, Micaela; Gimenez, Maria Ines; Tröetschel, Christian; D'alessandro, Celeste Paola; Poetsch, Ansgar; et al.; Proteomic study of the exponential-stationary growth phase transition in the haloarchaea Natrialba magadii and Haloferax volcanii; Wiley VCH Verlag; Proteomics (weinheim. Print); 18; 14; 7-2018; 1-41615-9853CONICET DigitalCONICETenginfo:eu-repo/semantics/altIdentifier/url/https://onlinelibrary.wiley.com/journal/16159861info:eu-repo/semantics/altIdentifier/doi/10.1002/pmic.201800116info:eu-repo/semantics/openAccesshttps://creativecommons.org/licenses/by-nc-sa/2.5/ar/reponame:CONICET Digital (CONICET)instname:Consejo Nacional de Investigaciones Científicas y Técnicas2025-10-15T15:12:01Zoai:ri.conicet.gov.ar:11336/88115instacron:CONICETInstitucionalhttp://ri.conicet.gov.ar/Organismo científico-tecnológicoNo correspondehttp://ri.conicet.gov.ar/oai/requestdasensio@conicet.gov.ar; lcarlino@conicet.gov.arArgentinaNo correspondeNo correspondeNo correspondeopendoar:34982025-10-15 15:12:02.141CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicasfalse
dc.title.none.fl_str_mv Proteomic study of the exponential-stationary growth phase transition in the haloarchaea Natrialba magadii and Haloferax volcanii
title Proteomic study of the exponential-stationary growth phase transition in the haloarchaea Natrialba magadii and Haloferax volcanii
spellingShingle Proteomic study of the exponential-stationary growth phase transition in the haloarchaea Natrialba magadii and Haloferax volcanii
Cerletti, Micaela
Comparative proteomics
Haloarchaea
Haloferax volcanii
Natrialba maagadii
Stationary phase
title_short Proteomic study of the exponential-stationary growth phase transition in the haloarchaea Natrialba magadii and Haloferax volcanii
title_full Proteomic study of the exponential-stationary growth phase transition in the haloarchaea Natrialba magadii and Haloferax volcanii
title_fullStr Proteomic study of the exponential-stationary growth phase transition in the haloarchaea Natrialba magadii and Haloferax volcanii
title_full_unstemmed Proteomic study of the exponential-stationary growth phase transition in the haloarchaea Natrialba magadii and Haloferax volcanii
title_sort Proteomic study of the exponential-stationary growth phase transition in the haloarchaea Natrialba magadii and Haloferax volcanii
dc.creator.none.fl_str_mv Cerletti, Micaela
Gimenez, Maria Ines
Tröetschel, Christian
D'alessandro, Celeste Paola
Poetsch, Ansgar
de Castro, Rosana Esther
Paggi, Roberto Alejandro
author Cerletti, Micaela
author_facet Cerletti, Micaela
Gimenez, Maria Ines
Tröetschel, Christian
D'alessandro, Celeste Paola
Poetsch, Ansgar
de Castro, Rosana Esther
Paggi, Roberto Alejandro
author_role author
author2 Gimenez, Maria Ines
Tröetschel, Christian
D'alessandro, Celeste Paola
Poetsch, Ansgar
de Castro, Rosana Esther
Paggi, Roberto Alejandro
author2_role author
author
author
author
author
author
dc.subject.none.fl_str_mv Comparative proteomics
Haloarchaea
Haloferax volcanii
Natrialba maagadii
Stationary phase
topic Comparative proteomics
Haloarchaea
Haloferax volcanii
Natrialba maagadii
Stationary phase
purl_subject.fl_str_mv https://purl.org/becyt/ford/1.6
https://purl.org/becyt/ford/1
dc.description.none.fl_txt_mv The dynamic changes that take place along the phases of microbial growth (lag, exponential, stationary, and death) have been widely studied in bacteria at the molecular and cellular levels, but little is known for archaea. In this study, a high-throughput approach was used to analyze and compare the proteomes of two haloarchaea during exponential and stationary growth: the neutrophilic Haloferax volcanii and the alkaliphilic Natrialba magadii. Almost 2000 proteins were identified in each species (≈50% of the predicted proteome). Among them, 532 and 432 were found to be differential between growth phases in H. volcanii and N. magadii, respectively. Changes upon entrance into stationary phase included an overall increase in proteins involved in the transport of small molecules and ions, stress response, and fatty acid catabolism. Proteins related to genetic processes and cell division showed a notorious decrease in amount. The data reported in this study not only contributes to our understanding of the exponential–stationary growth phase transition in extremophilic archaea but also provides the first comprehensive analysis of the proteome composition of N. magadii. The MS proteomics data have been deposited in the ProteomeXchange Consortium with the dataset identifier JPST000395.
Fil: Cerletti, Micaela. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Mar del Plata. Instituto de Investigaciones Biológicas. Universidad Nacional de Mar del Plata. Facultad de Ciencias Exactas y Naturales. Instituto de Investigaciones Biológicas; Argentina. Universidad Nacional de Mar del Plata; Argentina
Fil: Gimenez, Maria Ines. Universidad Nacional de Mar del Plata; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Mar del Plata. Instituto de Investigaciones Biológicas. Universidad Nacional de Mar del Plata. Facultad de Ciencias Exactas y Naturales. Instituto de Investigaciones Biológicas; Argentina
Fil: Tröetschel, Christian. Ruhr Universität Bochum; Alemania
Fil: D'alessandro, Celeste Paola. Universidade de Sao Paulo; Brasil
Fil: Poetsch, Ansgar. University Of Plymouth; . Ruhr Universität Bochum; Alemania
Fil: de Castro, Rosana Esther. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Mar del Plata. Instituto de Investigaciones Biológicas. Universidad Nacional de Mar del Plata. Facultad de Ciencias Exactas y Naturales. Instituto de Investigaciones Biológicas; Argentina. Universidad Nacional de Mar del Plata; Argentina
Fil: Paggi, Roberto Alejandro. Universidad Nacional de Mar del Plata; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Mar del Plata. Instituto de Investigaciones Biológicas. Universidad Nacional de Mar del Plata. Facultad de Ciencias Exactas y Naturales. Instituto de Investigaciones Biológicas; Argentina
description The dynamic changes that take place along the phases of microbial growth (lag, exponential, stationary, and death) have been widely studied in bacteria at the molecular and cellular levels, but little is known for archaea. In this study, a high-throughput approach was used to analyze and compare the proteomes of two haloarchaea during exponential and stationary growth: the neutrophilic Haloferax volcanii and the alkaliphilic Natrialba magadii. Almost 2000 proteins were identified in each species (≈50% of the predicted proteome). Among them, 532 and 432 were found to be differential between growth phases in H. volcanii and N. magadii, respectively. Changes upon entrance into stationary phase included an overall increase in proteins involved in the transport of small molecules and ions, stress response, and fatty acid catabolism. Proteins related to genetic processes and cell division showed a notorious decrease in amount. The data reported in this study not only contributes to our understanding of the exponential–stationary growth phase transition in extremophilic archaea but also provides the first comprehensive analysis of the proteome composition of N. magadii. The MS proteomics data have been deposited in the ProteomeXchange Consortium with the dataset identifier JPST000395.
publishDate 2018
dc.date.none.fl_str_mv 2018-07
dc.type.none.fl_str_mv info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
http://purl.org/coar/resource_type/c_6501
info:ar-repo/semantics/articulo
format article
status_str publishedVersion
dc.identifier.none.fl_str_mv http://hdl.handle.net/11336/88115
Cerletti, Micaela; Gimenez, Maria Ines; Tröetschel, Christian; D'alessandro, Celeste Paola; Poetsch, Ansgar; et al.; Proteomic study of the exponential-stationary growth phase transition in the haloarchaea Natrialba magadii and Haloferax volcanii; Wiley VCH Verlag; Proteomics (weinheim. Print); 18; 14; 7-2018; 1-4
1615-9853
CONICET Digital
CONICET
url http://hdl.handle.net/11336/88115
identifier_str_mv Cerletti, Micaela; Gimenez, Maria Ines; Tröetschel, Christian; D'alessandro, Celeste Paola; Poetsch, Ansgar; et al.; Proteomic study of the exponential-stationary growth phase transition in the haloarchaea Natrialba magadii and Haloferax volcanii; Wiley VCH Verlag; Proteomics (weinheim. Print); 18; 14; 7-2018; 1-4
1615-9853
CONICET Digital
CONICET
dc.language.none.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv info:eu-repo/semantics/altIdentifier/url/https://onlinelibrary.wiley.com/journal/16159861
info:eu-repo/semantics/altIdentifier/doi/10.1002/pmic.201800116
dc.rights.none.fl_str_mv info:eu-repo/semantics/openAccess
https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
eu_rights_str_mv openAccess
rights_invalid_str_mv https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
dc.format.none.fl_str_mv application/pdf
application/pdf
application/pdf
application/pdf
application/pdf
dc.publisher.none.fl_str_mv Wiley VCH Verlag
publisher.none.fl_str_mv Wiley VCH Verlag
dc.source.none.fl_str_mv reponame:CONICET Digital (CONICET)
instname:Consejo Nacional de Investigaciones Científicas y Técnicas
reponame_str CONICET Digital (CONICET)
collection CONICET Digital (CONICET)
instname_str Consejo Nacional de Investigaciones Científicas y Técnicas
repository.name.fl_str_mv CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicas
repository.mail.fl_str_mv dasensio@conicet.gov.ar; lcarlino@conicet.gov.ar
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