Isolation of Beauveria Bassiana from the Chagas disease vector Triatoma Infestans in the gran Chaco region of Argentina: Assessment of gene expression during host-pathogen interact...

Autores
Baldiviezo, Linda Vanesa; Pedrini, Nicolás; Santana, Marianela; Mannino, Maria Constanza; Nieva, Lucia Beatriz del Carmen; Gentile, Alberto Gerónimo; Cardozo, Rubén Marino
Año de publicación
2020
Idioma
inglés
Tipo de recurso
artículo
Estado
versión publicada
Descripción
A native strain of the entomopathogenic fungus Beauveria bassiana (Bb-C001) was isolated from a naturally infected Triatoma infestans, Klug (Hemiptera: Reduviidae) adult cadaver in the Gran Chaco region, Salta province, Argentina. The isolate was both phenotypic and molecularly characterized in a context of fungus-insect interaction, by measuring the expression pattern of toxin genes during infection and immune response of T. infestans. The commercial strain GHA of B. bassiana, which was previously used in field interventions to control these vectors, was used as reference in this study. The phylogenetic trees based on both ribosomal internal transcribed spacer (ITS) and elongation factor 1-alpha (EF1-α) indicated that Bb-C001 fits into a B. bassiana cluster, and the sequence-characterized amplified regions (SCAR) showed that Bb-C001 is different from the GHA strain. There were no differences between both strains regarding viability, radial growth, and conidia production, either in the median survival time or insect mortality. However, Bb-C001 showed a higher expression than GHA of the bassianolide synthetase gene (BbbslS) during infection, and similar levels of the beauvericin synthetase gene (BbbeaS). Immune-related genes of T. infestans nymphs (limpet-2 and defensin-1, -2, and -6) were later expressed and thus insects failed to stop the infection process. These results showed that B. bassiana Bb-C001 is a promised fungal strain to be incorporated in the current biological control programs of T. infestans in Salta province, Argentina.
Fil: Baldiviezo, Linda Vanesa. Universidad Nacional de Salta. Facultad de Ciencias Naturales; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - La Plata. Instituto de Investigaciones Bioquímicas de La Plata "Prof. Dr. Rodolfo R. Brenner". Universidad Nacional de la Plata. Facultad de Ciencias Médicas. Instituto de Investigaciones Bioquímicas de La Plata "Prof. Dr. Rodolfo R. Brenner"; Argentina. Provincia de Salta. Ministerio de Salud Pública; Argentina
Fil: Pedrini, Nicolás. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - La Plata. Instituto de Investigaciones Bioquímicas de La Plata "Prof. Dr. Rodolfo R. Brenner". Universidad Nacional de la Plata. Facultad de Ciencias Médicas. Instituto de Investigaciones Bioquímicas de La Plata "Prof. Dr. Rodolfo R. Brenner"; Argentina
Fil: Santana, Marianela. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - La Plata. Instituto de Investigaciones Bioquímicas de La Plata "Prof. Dr. Rodolfo R. Brenner". Universidad Nacional de la Plata. Facultad de Ciencias Médicas. Instituto de Investigaciones Bioquímicas de La Plata "Prof. Dr. Rodolfo R. Brenner"; Argentina
Fil: Mannino, Maria Constanza. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - La Plata. Instituto de Investigaciones Bioquímicas de La Plata "Prof. Dr. Rodolfo R. Brenner". Universidad Nacional de la Plata. Facultad de Ciencias Médicas. Instituto de Investigaciones Bioquímicas de La Plata "Prof. Dr. Rodolfo R. Brenner"; Argentina
Fil: Nieva, Lucia Beatriz del Carmen. Universidad Nacional de Salta. Facultad de Ciencias Naturales; Argentina
Fil: Gentile, Alberto Gerónimo. Provincia de Salta. Ministerio de Salud Pública; Argentina
Fil: Cardozo, Rubén Marino. Universidad Nacional de Salta. Facultad de Ciencias Naturales; Argentina. Provincia de Salta. Ministerio de Salud Pública; Argentina
Materia
BASSIANOLIDE
BEAUVERICIN
BIOLOGICAL CONTROL
DUAL GENE EXPRESSION
ENTOMOPATHOGENIC FUNGI
LIMPET
RESISTANT TRIATOMINES
Nivel de accesibilidad
acceso abierto
Condiciones de uso
https://creativecommons.org/licenses/by/2.5/ar/
Repositorio
CONICET Digital (CONICET)
Institución
Consejo Nacional de Investigaciones Científicas y Técnicas
OAI Identificador
oai:ri.conicet.gov.ar:11336/143641
Acceder
id CONICETDig_1e2df8e8b83397a68a5507642d25bd36
oai_identifier_str oai:ri.conicet.gov.ar:11336/143641
network_acronym_str CONICETDig
repository_id_str 3498
network_name_str CONICET Digital (CONICET)
spelling Isolation of Beauveria Bassiana from the Chagas disease vector Triatoma Infestans in the gran Chaco region of Argentina: Assessment of gene expression during host-pathogen interactionBaldiviezo, Linda VanesaPedrini, NicolásSantana, MarianelaMannino, Maria ConstanzaNieva, Lucia Beatriz del CarmenGentile, Alberto GerónimoCardozo, Rubén MarinoBASSIANOLIDEBEAUVERICINBIOLOGICAL CONTROLDUAL GENE EXPRESSIONENTOMOPATHOGENIC FUNGILIMPETRESISTANT TRIATOMINEShttps://purl.org/becyt/ford/1.6https://purl.org/becyt/ford/1A native strain of the entomopathogenic fungus Beauveria bassiana (Bb-C001) was isolated from a naturally infected Triatoma infestans, Klug (Hemiptera: Reduviidae) adult cadaver in the Gran Chaco region, Salta province, Argentina. The isolate was both phenotypic and molecularly characterized in a context of fungus-insect interaction, by measuring the expression pattern of toxin genes during infection and immune response of T. infestans. The commercial strain GHA of B. bassiana, which was previously used in field interventions to control these vectors, was used as reference in this study. The phylogenetic trees based on both ribosomal internal transcribed spacer (ITS) and elongation factor 1-alpha (EF1-α) indicated that Bb-C001 fits into a B. bassiana cluster, and the sequence-characterized amplified regions (SCAR) showed that Bb-C001 is different from the GHA strain. There were no differences between both strains regarding viability, radial growth, and conidia production, either in the median survival time or insect mortality. However, Bb-C001 showed a higher expression than GHA of the bassianolide synthetase gene (BbbslS) during infection, and similar levels of the beauvericin synthetase gene (BbbeaS). Immune-related genes of T. infestans nymphs (limpet-2 and defensin-1, -2, and -6) were later expressed and thus insects failed to stop the infection process. These results showed that B. bassiana Bb-C001 is a promised fungal strain to be incorporated in the current biological control programs of T. infestans in Salta province, Argentina.Fil: Baldiviezo, Linda Vanesa. Universidad Nacional de Salta. Facultad de Ciencias Naturales; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - La Plata. Instituto de Investigaciones Bioquímicas de La Plata "Prof. Dr. Rodolfo R. Brenner". Universidad Nacional de la Plata. Facultad de Ciencias Médicas. Instituto de Investigaciones Bioquímicas de La Plata "Prof. Dr. Rodolfo R. Brenner"; Argentina. Provincia de Salta. Ministerio de Salud Pública; ArgentinaFil: Pedrini, Nicolás. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - La Plata. Instituto de Investigaciones Bioquímicas de La Plata "Prof. Dr. Rodolfo R. Brenner". Universidad Nacional de la Plata. Facultad de Ciencias Médicas. Instituto de Investigaciones Bioquímicas de La Plata "Prof. Dr. Rodolfo R. Brenner"; ArgentinaFil: Santana, Marianela. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - La Plata. Instituto de Investigaciones Bioquímicas de La Plata "Prof. Dr. Rodolfo R. Brenner". Universidad Nacional de la Plata. Facultad de Ciencias Médicas. Instituto de Investigaciones Bioquímicas de La Plata "Prof. Dr. Rodolfo R. Brenner"; ArgentinaFil: Mannino, Maria Constanza. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - La Plata. Instituto de Investigaciones Bioquímicas de La Plata "Prof. Dr. Rodolfo R. Brenner". Universidad Nacional de la Plata. Facultad de Ciencias Médicas. Instituto de Investigaciones Bioquímicas de La Plata "Prof. Dr. Rodolfo R. Brenner"; ArgentinaFil: Nieva, Lucia Beatriz del Carmen. Universidad Nacional de Salta. Facultad de Ciencias Naturales; ArgentinaFil: Gentile, Alberto Gerónimo. Provincia de Salta. Ministerio de Salud Pública; ArgentinaFil: Cardozo, Rubén Marino. Universidad Nacional de Salta. Facultad de Ciencias Naturales; Argentina. Provincia de Salta. Ministerio de Salud Pública; ArgentinaMDPI AG2020-10-12info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionhttp://purl.org/coar/resource_type/c_6501info:ar-repo/semantics/articuloapplication/pdfapplication/pdfapplication/pdfapplication/pdfapplication/pdfhttp://hdl.handle.net/11336/143641Baldiviezo, Linda Vanesa; Pedrini, Nicolás; Santana, Marianela; Mannino, Maria Constanza; Nieva, Lucia Beatriz del Carmen; et al.; Isolation of Beauveria Bassiana from the Chagas disease vector Triatoma Infestans in the gran Chaco region of Argentina: Assessment of gene expression during host-pathogen interaction; MDPI AG; Journal of Fungi; 6; 4; 12-10-2020; 1-132309-608XCONICET DigitalCONICETenginfo:eu-repo/semantics/altIdentifier/doi/10.3390/jof6040219info:eu-repo/semantics/altIdentifier/url/https://www.mdpi.com/2309-608X/6/4/219info:eu-repo/semantics/openAccesshttps://creativecommons.org/licenses/by/2.5/ar/reponame:CONICET Digital (CONICET)instname:Consejo Nacional de Investigaciones Científicas y Técnicas2025-09-17T11:32:40Zoai:ri.conicet.gov.ar:11336/143641instacron:CONICETInstitucionalhttp://ri.conicet.gov.ar/Organismo científico-tecnológicoNo correspondehttp://ri.conicet.gov.ar/oai/requestdasensio@conicet.gov.ar; lcarlino@conicet.gov.arArgentinaNo correspondeNo correspondeNo correspondeopendoar:34982025-09-17 11:32:40.491CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicasfalse
dc.title.none.fl_str_mv Isolation of Beauveria Bassiana from the Chagas disease vector Triatoma Infestans in the gran Chaco region of Argentina: Assessment of gene expression during host-pathogen interaction
title Isolation of Beauveria Bassiana from the Chagas disease vector Triatoma Infestans in the gran Chaco region of Argentina: Assessment of gene expression during host-pathogen interaction
spellingShingle Isolation of Beauveria Bassiana from the Chagas disease vector Triatoma Infestans in the gran Chaco region of Argentina: Assessment of gene expression during host-pathogen interaction
Baldiviezo, Linda Vanesa
BASSIANOLIDE
BEAUVERICIN
BIOLOGICAL CONTROL
DUAL GENE EXPRESSION
ENTOMOPATHOGENIC FUNGI
LIMPET
RESISTANT TRIATOMINES
title_short Isolation of Beauveria Bassiana from the Chagas disease vector Triatoma Infestans in the gran Chaco region of Argentina: Assessment of gene expression during host-pathogen interaction
title_full Isolation of Beauveria Bassiana from the Chagas disease vector Triatoma Infestans in the gran Chaco region of Argentina: Assessment of gene expression during host-pathogen interaction
title_fullStr Isolation of Beauveria Bassiana from the Chagas disease vector Triatoma Infestans in the gran Chaco region of Argentina: Assessment of gene expression during host-pathogen interaction
title_full_unstemmed Isolation of Beauveria Bassiana from the Chagas disease vector Triatoma Infestans in the gran Chaco region of Argentina: Assessment of gene expression during host-pathogen interaction
title_sort Isolation of Beauveria Bassiana from the Chagas disease vector Triatoma Infestans in the gran Chaco region of Argentina: Assessment of gene expression during host-pathogen interaction
dc.creator.none.fl_str_mv Baldiviezo, Linda Vanesa
Pedrini, Nicolás
Santana, Marianela
Mannino, Maria Constanza
Nieva, Lucia Beatriz del Carmen
Gentile, Alberto Gerónimo
Cardozo, Rubén Marino
author Baldiviezo, Linda Vanesa
author_facet Baldiviezo, Linda Vanesa
Pedrini, Nicolás
Santana, Marianela
Mannino, Maria Constanza
Nieva, Lucia Beatriz del Carmen
Gentile, Alberto Gerónimo
Cardozo, Rubén Marino
author_role author
author2 Pedrini, Nicolás
Santana, Marianela
Mannino, Maria Constanza
Nieva, Lucia Beatriz del Carmen
Gentile, Alberto Gerónimo
Cardozo, Rubén Marino
author2_role author
author
author
author
author
author
dc.subject.none.fl_str_mv BASSIANOLIDE
BEAUVERICIN
BIOLOGICAL CONTROL
DUAL GENE EXPRESSION
ENTOMOPATHOGENIC FUNGI
LIMPET
RESISTANT TRIATOMINES
topic BASSIANOLIDE
BEAUVERICIN
BIOLOGICAL CONTROL
DUAL GENE EXPRESSION
ENTOMOPATHOGENIC FUNGI
LIMPET
RESISTANT TRIATOMINES
purl_subject.fl_str_mv https://purl.org/becyt/ford/1.6
https://purl.org/becyt/ford/1
dc.description.none.fl_txt_mv A native strain of the entomopathogenic fungus Beauveria bassiana (Bb-C001) was isolated from a naturally infected Triatoma infestans, Klug (Hemiptera: Reduviidae) adult cadaver in the Gran Chaco region, Salta province, Argentina. The isolate was both phenotypic and molecularly characterized in a context of fungus-insect interaction, by measuring the expression pattern of toxin genes during infection and immune response of T. infestans. The commercial strain GHA of B. bassiana, which was previously used in field interventions to control these vectors, was used as reference in this study. The phylogenetic trees based on both ribosomal internal transcribed spacer (ITS) and elongation factor 1-alpha (EF1-α) indicated that Bb-C001 fits into a B. bassiana cluster, and the sequence-characterized amplified regions (SCAR) showed that Bb-C001 is different from the GHA strain. There were no differences between both strains regarding viability, radial growth, and conidia production, either in the median survival time or insect mortality. However, Bb-C001 showed a higher expression than GHA of the bassianolide synthetase gene (BbbslS) during infection, and similar levels of the beauvericin synthetase gene (BbbeaS). Immune-related genes of T. infestans nymphs (limpet-2 and defensin-1, -2, and -6) were later expressed and thus insects failed to stop the infection process. These results showed that B. bassiana Bb-C001 is a promised fungal strain to be incorporated in the current biological control programs of T. infestans in Salta province, Argentina.
Fil: Baldiviezo, Linda Vanesa. Universidad Nacional de Salta. Facultad de Ciencias Naturales; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - La Plata. Instituto de Investigaciones Bioquímicas de La Plata "Prof. Dr. Rodolfo R. Brenner". Universidad Nacional de la Plata. Facultad de Ciencias Médicas. Instituto de Investigaciones Bioquímicas de La Plata "Prof. Dr. Rodolfo R. Brenner"; Argentina. Provincia de Salta. Ministerio de Salud Pública; Argentina
Fil: Pedrini, Nicolás. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - La Plata. Instituto de Investigaciones Bioquímicas de La Plata "Prof. Dr. Rodolfo R. Brenner". Universidad Nacional de la Plata. Facultad de Ciencias Médicas. Instituto de Investigaciones Bioquímicas de La Plata "Prof. Dr. Rodolfo R. Brenner"; Argentina
Fil: Santana, Marianela. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - La Plata. Instituto de Investigaciones Bioquímicas de La Plata "Prof. Dr. Rodolfo R. Brenner". Universidad Nacional de la Plata. Facultad de Ciencias Médicas. Instituto de Investigaciones Bioquímicas de La Plata "Prof. Dr. Rodolfo R. Brenner"; Argentina
Fil: Mannino, Maria Constanza. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - La Plata. Instituto de Investigaciones Bioquímicas de La Plata "Prof. Dr. Rodolfo R. Brenner". Universidad Nacional de la Plata. Facultad de Ciencias Médicas. Instituto de Investigaciones Bioquímicas de La Plata "Prof. Dr. Rodolfo R. Brenner"; Argentina
Fil: Nieva, Lucia Beatriz del Carmen. Universidad Nacional de Salta. Facultad de Ciencias Naturales; Argentina
Fil: Gentile, Alberto Gerónimo. Provincia de Salta. Ministerio de Salud Pública; Argentina
Fil: Cardozo, Rubén Marino. Universidad Nacional de Salta. Facultad de Ciencias Naturales; Argentina. Provincia de Salta. Ministerio de Salud Pública; Argentina
description A native strain of the entomopathogenic fungus Beauveria bassiana (Bb-C001) was isolated from a naturally infected Triatoma infestans, Klug (Hemiptera: Reduviidae) adult cadaver in the Gran Chaco region, Salta province, Argentina. The isolate was both phenotypic and molecularly characterized in a context of fungus-insect interaction, by measuring the expression pattern of toxin genes during infection and immune response of T. infestans. The commercial strain GHA of B. bassiana, which was previously used in field interventions to control these vectors, was used as reference in this study. The phylogenetic trees based on both ribosomal internal transcribed spacer (ITS) and elongation factor 1-alpha (EF1-α) indicated that Bb-C001 fits into a B. bassiana cluster, and the sequence-characterized amplified regions (SCAR) showed that Bb-C001 is different from the GHA strain. There were no differences between both strains regarding viability, radial growth, and conidia production, either in the median survival time or insect mortality. However, Bb-C001 showed a higher expression than GHA of the bassianolide synthetase gene (BbbslS) during infection, and similar levels of the beauvericin synthetase gene (BbbeaS). Immune-related genes of T. infestans nymphs (limpet-2 and defensin-1, -2, and -6) were later expressed and thus insects failed to stop the infection process. These results showed that B. bassiana Bb-C001 is a promised fungal strain to be incorporated in the current biological control programs of T. infestans in Salta province, Argentina.
publishDate 2020
dc.date.none.fl_str_mv 2020-10-12
dc.type.none.fl_str_mv info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
http://purl.org/coar/resource_type/c_6501
info:ar-repo/semantics/articulo
format article
status_str publishedVersion
dc.identifier.none.fl_str_mv http://hdl.handle.net/11336/143641
Baldiviezo, Linda Vanesa; Pedrini, Nicolás; Santana, Marianela; Mannino, Maria Constanza; Nieva, Lucia Beatriz del Carmen; et al.; Isolation of Beauveria Bassiana from the Chagas disease vector Triatoma Infestans in the gran Chaco region of Argentina: Assessment of gene expression during host-pathogen interaction; MDPI AG; Journal of Fungi; 6; 4; 12-10-2020; 1-13
2309-608X
CONICET Digital
CONICET
url http://hdl.handle.net/11336/143641
identifier_str_mv Baldiviezo, Linda Vanesa; Pedrini, Nicolás; Santana, Marianela; Mannino, Maria Constanza; Nieva, Lucia Beatriz del Carmen; et al.; Isolation of Beauveria Bassiana from the Chagas disease vector Triatoma Infestans in the gran Chaco region of Argentina: Assessment of gene expression during host-pathogen interaction; MDPI AG; Journal of Fungi; 6; 4; 12-10-2020; 1-13
2309-608X
CONICET Digital
CONICET
dc.language.none.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv info:eu-repo/semantics/altIdentifier/doi/10.3390/jof6040219
info:eu-repo/semantics/altIdentifier/url/https://www.mdpi.com/2309-608X/6/4/219
dc.rights.none.fl_str_mv info:eu-repo/semantics/openAccess
https://creativecommons.org/licenses/by/2.5/ar/
eu_rights_str_mv openAccess
rights_invalid_str_mv https://creativecommons.org/licenses/by/2.5/ar/
dc.format.none.fl_str_mv application/pdf
application/pdf
application/pdf
application/pdf
application/pdf
dc.publisher.none.fl_str_mv MDPI AG
publisher.none.fl_str_mv MDPI AG
dc.source.none.fl_str_mv reponame:CONICET Digital (CONICET)
instname:Consejo Nacional de Investigaciones Científicas y Técnicas
reponame_str CONICET Digital (CONICET)
collection CONICET Digital (CONICET)
instname_str Consejo Nacional de Investigaciones Científicas y Técnicas
repository.name.fl_str_mv CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicas
repository.mail.fl_str_mv dasensio@conicet.gov.ar; lcarlino@conicet.gov.ar
_version_ 1843606687249858560
score 13.001348

Publicaciones similares