Human Breast Tumour Effect Cells Viability of African Dioscorea rotundata Tuber Extracts in MCF-7 and MDA-MB231 Cell Lines

Autores
Odimegwu, Joy; Odukoya Olukemi; Español, Alejandro Javier; Rosales, Eliana María
Año de publicación
2022
Idioma
inglés
Tipo de recurso
artículo
Estado
versión publicada
Descripción
Objective: We aim to test the efficacy of edible Dioscorea species grown and consumed in Nigeria, Africa on two breast cancer cell lines; MCF-7 and MDAMB231 derived from a luminal and a Triple-Negative Breast Cancer (TNBC) respectively and to confirm safety in non-tumour cells MCF-10A using a wellestablished cytotoxic compound paclitaxel as a standard. Metastatic breast cancer is a prevalent cause of mortality in women around the world. Breast cancer therapies have greatly advanced in recent years, but many patients develop cancer re-occurrence and metastasis and subsequently yield to the disease because of chemoresistance. Methods: Ethanolic extracts of Dioscorea rotundata boiled and raw (DiosB and DiosR) respectively were chemically analyzed for the presence of diosgenin using HPLC and the cytotoxic activities of the extracts were tested on MCF-7, MDA-MB-231 and MCF-10 A cells In vitro by MTT assay. Results: DiosB and DiosR extracts showed a higher maximal effect on MCF-7 cells than on MDA-MB231 after 24 h and 48 h treatments (p<0.0001 and p<0.05 respectively). DiosR, if applied at a range between 50-70 g/ml, can be effective to reduce breast tumor cell viability without affecting non tumorigenic MCF-10 A cells either at 24 h or at 48 h. DiosB showed an IC50 of 38.83 µg/ml while DiosR showed an IC50 of 41.80 µg/ml. Conclusion: These results show that ethanolic extracts of Dioscorea rotundata tubers could be used effectively to treat breast cancer tumors and this is in sync with its diosgenin content as other Dioscorea species applied for similar treatments in Asia and elsewhere.
Fil: Odimegwu, Joy. University Of Lagos; Nigeria
Fil: Odukoya Olukemi. University Of Lagos; Nigeria
Fil: Español, Alejandro Javier. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Houssay. Centro de Estudios Farmacológicos y Botánicos. Universidad de Buenos Aires. Facultad de Medicina. Centro de Estudios Farmacológicos y Botánicos; Argentina
Fil: Rosales, Eliana María. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Houssay. Centro de Estudios Farmacológicos y Botánicos. Universidad de Buenos Aires. Facultad de Medicina. Centro de Estudios Farmacológicos y Botánicos; Argentina
Materia
NATURAL DRUGS
BREAST CANCER
DIOSCOREA ROTUNDATA
EXTRACTS
Nivel de accesibilidad
acceso abierto
Condiciones de uso
https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
Repositorio
CONICET Digital (CONICET)
Institución
Consejo Nacional de Investigaciones Científicas y Técnicas
OAI Identificador
oai:ri.conicet.gov.ar:11336/215515
Acceder
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network_name_str CONICET Digital (CONICET)
spelling Human Breast Tumour Effect Cells Viability of African Dioscorea rotundata Tuber Extracts in MCF-7 and MDA-MB231 Cell LinesOdimegwu, JoyOdukoya OlukemiEspañol, Alejandro JavierRosales, Eliana MaríaNATURAL DRUGSBREAST CANCERDIOSCOREA ROTUNDATAEXTRACTShttps://purl.org/becyt/ford/3.3https://purl.org/becyt/ford/3Objective: We aim to test the efficacy of edible Dioscorea species grown and consumed in Nigeria, Africa on two breast cancer cell lines; MCF-7 and MDAMB231 derived from a luminal and a Triple-Negative Breast Cancer (TNBC) respectively and to confirm safety in non-tumour cells MCF-10A using a wellestablished cytotoxic compound paclitaxel as a standard. Metastatic breast cancer is a prevalent cause of mortality in women around the world. Breast cancer therapies have greatly advanced in recent years, but many patients develop cancer re-occurrence and metastasis and subsequently yield to the disease because of chemoresistance. Methods: Ethanolic extracts of Dioscorea rotundata boiled and raw (DiosB and DiosR) respectively were chemically analyzed for the presence of diosgenin using HPLC and the cytotoxic activities of the extracts were tested on MCF-7, MDA-MB-231 and MCF-10 A cells In vitro by MTT assay. Results: DiosB and DiosR extracts showed a higher maximal effect on MCF-7 cells than on MDA-MB231 after 24 h and 48 h treatments (p<0.0001 and p<0.05 respectively). DiosR, if applied at a range between 50-70 g/ml, can be effective to reduce breast tumor cell viability without affecting non tumorigenic MCF-10 A cells either at 24 h or at 48 h. DiosB showed an IC50 of 38.83 µg/ml while DiosR showed an IC50 of 41.80 µg/ml. Conclusion: These results show that ethanolic extracts of Dioscorea rotundata tubers could be used effectively to treat breast cancer tumors and this is in sync with its diosgenin content as other Dioscorea species applied for similar treatments in Asia and elsewhere.Fil: Odimegwu, Joy. University Of Lagos; NigeriaFil: Odukoya Olukemi. University Of Lagos; NigeriaFil: Español, Alejandro Javier. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Houssay. Centro de Estudios Farmacológicos y Botánicos. Universidad de Buenos Aires. Facultad de Medicina. Centro de Estudios Farmacológicos y Botánicos; ArgentinaFil: Rosales, Eliana María. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Houssay. Centro de Estudios Farmacológicos y Botánicos. Universidad de Buenos Aires. Facultad de Medicina. Centro de Estudios Farmacológicos y Botánicos; ArgentinaRROIJ-Open Access Journals Fze2022-03info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionhttp://purl.org/coar/resource_type/c_6501info:ar-repo/semantics/articuloapplication/pdfapplication/pdfhttp://hdl.handle.net/11336/215515Odimegwu, Joy; Odukoya Olukemi; Español, Alejandro Javier; Rosales, Eliana María; Human Breast Tumour Effect Cells Viability of African Dioscorea rotundata Tuber Extracts in MCF-7 and MDA-MB231 Cell Lines; RROIJ-Open Access Journals Fze; Reports in Cancer Treatment; 6; 2; 3-2022; 1-120361-5960CONICET DigitalCONICETenginfo:eu-repo/semantics/altIdentifier/url/https://www.rroij.com/open-access/human-breast-tumour-effect-cells-viability-of-african-dioscorea-rotundata-tuber-extracts-in-mcf7-and-mdamb231-cell-lines.php?aid=91019info:eu-repo/semantics/openAccesshttps://creativecommons.org/licenses/by-nc-sa/2.5/ar/reponame:CONICET Digital (CONICET)instname:Consejo Nacional de Investigaciones Científicas y Técnicas2025-09-10T13:25:07Zoai:ri.conicet.gov.ar:11336/215515instacron:CONICETInstitucionalhttp://ri.conicet.gov.ar/Organismo científico-tecnológicoNo correspondehttp://ri.conicet.gov.ar/oai/requestdasensio@conicet.gov.ar; lcarlino@conicet.gov.arArgentinaNo correspondeNo correspondeNo correspondeopendoar:34982025-09-10 13:25:07.692CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicasfalse
dc.title.none.fl_str_mv Human Breast Tumour Effect Cells Viability of African Dioscorea rotundata Tuber Extracts in MCF-7 and MDA-MB231 Cell Lines
title Human Breast Tumour Effect Cells Viability of African Dioscorea rotundata Tuber Extracts in MCF-7 and MDA-MB231 Cell Lines
spellingShingle Human Breast Tumour Effect Cells Viability of African Dioscorea rotundata Tuber Extracts in MCF-7 and MDA-MB231 Cell Lines
Odimegwu, Joy
NATURAL DRUGS
BREAST CANCER
DIOSCOREA ROTUNDATA
EXTRACTS
title_short Human Breast Tumour Effect Cells Viability of African Dioscorea rotundata Tuber Extracts in MCF-7 and MDA-MB231 Cell Lines
title_full Human Breast Tumour Effect Cells Viability of African Dioscorea rotundata Tuber Extracts in MCF-7 and MDA-MB231 Cell Lines
title_fullStr Human Breast Tumour Effect Cells Viability of African Dioscorea rotundata Tuber Extracts in MCF-7 and MDA-MB231 Cell Lines
title_full_unstemmed Human Breast Tumour Effect Cells Viability of African Dioscorea rotundata Tuber Extracts in MCF-7 and MDA-MB231 Cell Lines
title_sort Human Breast Tumour Effect Cells Viability of African Dioscorea rotundata Tuber Extracts in MCF-7 and MDA-MB231 Cell Lines
dc.creator.none.fl_str_mv Odimegwu, Joy
Odukoya Olukemi
Español, Alejandro Javier
Rosales, Eliana María
author Odimegwu, Joy
author_facet Odimegwu, Joy
Odukoya Olukemi
Español, Alejandro Javier
Rosales, Eliana María
author_role author
author2 Odukoya Olukemi
Español, Alejandro Javier
Rosales, Eliana María
author2_role author
author
author
dc.subject.none.fl_str_mv NATURAL DRUGS
BREAST CANCER
DIOSCOREA ROTUNDATA
EXTRACTS
topic NATURAL DRUGS
BREAST CANCER
DIOSCOREA ROTUNDATA
EXTRACTS
purl_subject.fl_str_mv https://purl.org/becyt/ford/3.3
https://purl.org/becyt/ford/3
dc.description.none.fl_txt_mv Objective: We aim to test the efficacy of edible Dioscorea species grown and consumed in Nigeria, Africa on two breast cancer cell lines; MCF-7 and MDAMB231 derived from a luminal and a Triple-Negative Breast Cancer (TNBC) respectively and to confirm safety in non-tumour cells MCF-10A using a wellestablished cytotoxic compound paclitaxel as a standard. Metastatic breast cancer is a prevalent cause of mortality in women around the world. Breast cancer therapies have greatly advanced in recent years, but many patients develop cancer re-occurrence and metastasis and subsequently yield to the disease because of chemoresistance. Methods: Ethanolic extracts of Dioscorea rotundata boiled and raw (DiosB and DiosR) respectively were chemically analyzed for the presence of diosgenin using HPLC and the cytotoxic activities of the extracts were tested on MCF-7, MDA-MB-231 and MCF-10 A cells In vitro by MTT assay. Results: DiosB and DiosR extracts showed a higher maximal effect on MCF-7 cells than on MDA-MB231 after 24 h and 48 h treatments (p<0.0001 and p<0.05 respectively). DiosR, if applied at a range between 50-70 g/ml, can be effective to reduce breast tumor cell viability without affecting non tumorigenic MCF-10 A cells either at 24 h or at 48 h. DiosB showed an IC50 of 38.83 µg/ml while DiosR showed an IC50 of 41.80 µg/ml. Conclusion: These results show that ethanolic extracts of Dioscorea rotundata tubers could be used effectively to treat breast cancer tumors and this is in sync with its diosgenin content as other Dioscorea species applied for similar treatments in Asia and elsewhere.
Fil: Odimegwu, Joy. University Of Lagos; Nigeria
Fil: Odukoya Olukemi. University Of Lagos; Nigeria
Fil: Español, Alejandro Javier. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Houssay. Centro de Estudios Farmacológicos y Botánicos. Universidad de Buenos Aires. Facultad de Medicina. Centro de Estudios Farmacológicos y Botánicos; Argentina
Fil: Rosales, Eliana María. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Houssay. Centro de Estudios Farmacológicos y Botánicos. Universidad de Buenos Aires. Facultad de Medicina. Centro de Estudios Farmacológicos y Botánicos; Argentina
description Objective: We aim to test the efficacy of edible Dioscorea species grown and consumed in Nigeria, Africa on two breast cancer cell lines; MCF-7 and MDAMB231 derived from a luminal and a Triple-Negative Breast Cancer (TNBC) respectively and to confirm safety in non-tumour cells MCF-10A using a wellestablished cytotoxic compound paclitaxel as a standard. Metastatic breast cancer is a prevalent cause of mortality in women around the world. Breast cancer therapies have greatly advanced in recent years, but many patients develop cancer re-occurrence and metastasis and subsequently yield to the disease because of chemoresistance. Methods: Ethanolic extracts of Dioscorea rotundata boiled and raw (DiosB and DiosR) respectively were chemically analyzed for the presence of diosgenin using HPLC and the cytotoxic activities of the extracts were tested on MCF-7, MDA-MB-231 and MCF-10 A cells In vitro by MTT assay. Results: DiosB and DiosR extracts showed a higher maximal effect on MCF-7 cells than on MDA-MB231 after 24 h and 48 h treatments (p<0.0001 and p<0.05 respectively). DiosR, if applied at a range between 50-70 g/ml, can be effective to reduce breast tumor cell viability without affecting non tumorigenic MCF-10 A cells either at 24 h or at 48 h. DiosB showed an IC50 of 38.83 µg/ml while DiosR showed an IC50 of 41.80 µg/ml. Conclusion: These results show that ethanolic extracts of Dioscorea rotundata tubers could be used effectively to treat breast cancer tumors and this is in sync with its diosgenin content as other Dioscorea species applied for similar treatments in Asia and elsewhere.
publishDate 2022
dc.date.none.fl_str_mv 2022-03
dc.type.none.fl_str_mv info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
http://purl.org/coar/resource_type/c_6501
info:ar-repo/semantics/articulo
format article
status_str publishedVersion
dc.identifier.none.fl_str_mv http://hdl.handle.net/11336/215515
Odimegwu, Joy; Odukoya Olukemi; Español, Alejandro Javier; Rosales, Eliana María; Human Breast Tumour Effect Cells Viability of African Dioscorea rotundata Tuber Extracts in MCF-7 and MDA-MB231 Cell Lines; RROIJ-Open Access Journals Fze; Reports in Cancer Treatment; 6; 2; 3-2022; 1-12
0361-5960
CONICET Digital
CONICET
url http://hdl.handle.net/11336/215515
identifier_str_mv Odimegwu, Joy; Odukoya Olukemi; Español, Alejandro Javier; Rosales, Eliana María; Human Breast Tumour Effect Cells Viability of African Dioscorea rotundata Tuber Extracts in MCF-7 and MDA-MB231 Cell Lines; RROIJ-Open Access Journals Fze; Reports in Cancer Treatment; 6; 2; 3-2022; 1-12
0361-5960
CONICET Digital
CONICET
dc.language.none.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv info:eu-repo/semantics/altIdentifier/url/https://www.rroij.com/open-access/human-breast-tumour-effect-cells-viability-of-african-dioscorea-rotundata-tuber-extracts-in-mcf7-and-mdamb231-cell-lines.php?aid=91019
dc.rights.none.fl_str_mv info:eu-repo/semantics/openAccess
https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
eu_rights_str_mv openAccess
rights_invalid_str_mv https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
dc.format.none.fl_str_mv application/pdf
application/pdf
dc.publisher.none.fl_str_mv RROIJ-Open Access Journals Fze
publisher.none.fl_str_mv RROIJ-Open Access Journals Fze
dc.source.none.fl_str_mv reponame:CONICET Digital (CONICET)
instname:Consejo Nacional de Investigaciones Científicas y Técnicas
reponame_str CONICET Digital (CONICET)
collection CONICET Digital (CONICET)
instname_str Consejo Nacional de Investigaciones Científicas y Técnicas
repository.name.fl_str_mv CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicas
repository.mail.fl_str_mv dasensio@conicet.gov.ar; lcarlino@conicet.gov.ar
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score 12.48226

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