Six species of Diaporthe associated with Phomopsis stem canker of sunflower in southern Pampean region of Argentina
- Autores
- Zambelli, Andres Daniel; Mancebo, María F.; Bazzalo, María E.; Reid, Roberto J.; Sanchez, María C.; Kontz, Brian J.; Mathew, Febina M.
- Año de publicación
- 2021
- Idioma
- inglés
- Tipo de recurso
- artículo
- Estado
- versión publicada
- Descripción
- A survey of 67 commercial fields in 19 locations was conducted in the southern Pampean region of Argentina for Phomopsis stem canker of sunflower (Helianthus annuus L.) between 2014 and 2019. A total of 210 plants having typical symptoms of Phomopsis stem canker were randomly sampled, and fungal isolation was performed. Of the 187 isolates of Diaporthe that were recovered, 94% of the isolates showed morphological characteristics similar to D. helianthi, 3% to D. gulyae, 1% to D. caulivora, 1% to D. sojae, 0.5% to D. kongii, and 0.5% to D. longicolla. Following morphological characterization, the identity of the six morpho-species was confirmed by phylogenetic analyses of b-tubulin, translation elongation factor 1-a, and internal transcribed spacer gene regions. Koch’s postulates were completed for the six fungi by inoculating one susceptible sunflower hybrid with one isolate each of the six species of Diaporthe using the stem-wound inoculation method. Seven days postinoculation, significant differences in disease severity were observed between the six isolates (P < 0.0001), with D. helianthi and D. gulyae isolates causing significantly greater disease severity. To our knowledge, this is the first report of D. kongii, D. longicolla, D. caulivora, and D. sojae associated with Phomopsis stem canker of sunflower in Argentina.
Fil: Zambelli, Andres Daniel. Universidad Nacional de Mar del Plata. Facultad de Ciencias Agrarias; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Mar del Plata; Argentina
Fil: Mancebo, María F.. Advanta Semillas S.A.I.C.; Argentina
Fil: Bazzalo, María E.. Advanta Semillas S.A.I.C.; Argentina
Fil: Reid, Roberto J.. Advanta Semillas S.A.I.C.; Argentina
Fil: Sanchez, María C.. Universidad Nacional de Mar del Plata. Facultad de Ciencias Agrarias; Argentina
Fil: Kontz, Brian J.. South Dakota State University; Estados Unidos
Fil: Mathew, Febina M.. South Dakota State University; Estados Unidos - Materia
-
ARGENTINA
DIAPORTHE
PHOMOPSIS STEM CANKER
SUNFLOWER - Nivel de accesibilidad
- acceso abierto
- Condiciones de uso
- https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
- Repositorio
- Institución
- Consejo Nacional de Investigaciones Científicas y Técnicas
- OAI Identificador
- oai:ri.conicet.gov.ar:11336/167523
Ver los metadatos del registro completo
id |
CONICETDig_1d01ca11070610eb6de8fceb8861bc24 |
---|---|
oai_identifier_str |
oai:ri.conicet.gov.ar:11336/167523 |
network_acronym_str |
CONICETDig |
repository_id_str |
3498 |
network_name_str |
CONICET Digital (CONICET) |
spelling |
Six species of Diaporthe associated with Phomopsis stem canker of sunflower in southern Pampean region of ArgentinaZambelli, Andres DanielMancebo, María F.Bazzalo, María E.Reid, Roberto J.Sanchez, María C.Kontz, Brian J.Mathew, Febina M.ARGENTINADIAPORTHEPHOMOPSIS STEM CANKERSUNFLOWERhttps://purl.org/becyt/ford/4.1https://purl.org/becyt/ford/4A survey of 67 commercial fields in 19 locations was conducted in the southern Pampean region of Argentina for Phomopsis stem canker of sunflower (Helianthus annuus L.) between 2014 and 2019. A total of 210 plants having typical symptoms of Phomopsis stem canker were randomly sampled, and fungal isolation was performed. Of the 187 isolates of Diaporthe that were recovered, 94% of the isolates showed morphological characteristics similar to D. helianthi, 3% to D. gulyae, 1% to D. caulivora, 1% to D. sojae, 0.5% to D. kongii, and 0.5% to D. longicolla. Following morphological characterization, the identity of the six morpho-species was confirmed by phylogenetic analyses of b-tubulin, translation elongation factor 1-a, and internal transcribed spacer gene regions. Koch’s postulates were completed for the six fungi by inoculating one susceptible sunflower hybrid with one isolate each of the six species of Diaporthe using the stem-wound inoculation method. Seven days postinoculation, significant differences in disease severity were observed between the six isolates (P < 0.0001), with D. helianthi and D. gulyae isolates causing significantly greater disease severity. To our knowledge, this is the first report of D. kongii, D. longicolla, D. caulivora, and D. sojae associated with Phomopsis stem canker of sunflower in Argentina.Fil: Zambelli, Andres Daniel. Universidad Nacional de Mar del Plata. Facultad de Ciencias Agrarias; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Mar del Plata; ArgentinaFil: Mancebo, María F.. Advanta Semillas S.A.I.C.; ArgentinaFil: Bazzalo, María E.. Advanta Semillas S.A.I.C.; ArgentinaFil: Reid, Roberto J.. Advanta Semillas S.A.I.C.; ArgentinaFil: Sanchez, María C.. Universidad Nacional de Mar del Plata. Facultad de Ciencias Agrarias; ArgentinaFil: Kontz, Brian J.. South Dakota State University; Estados UnidosFil: Mathew, Febina M.. South Dakota State University; Estados UnidosAmerican Phytopathological Society2021-01info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionhttp://purl.org/coar/resource_type/c_6501info:ar-repo/semantics/articuloapplication/pdfapplication/pdfhttp://hdl.handle.net/11336/167523Zambelli, Andres Daniel; Mancebo, María F.; Bazzalo, María E.; Reid, Roberto J.; Sanchez, María C.; et al.; Six species of Diaporthe associated with Phomopsis stem canker of sunflower in southern Pampean region of Argentina; American Phytopathological Society; Plant Health Progess; 22; 2; 1-2021; 136-1421535-1025CONICET DigitalCONICETenginfo:eu-repo/semantics/altIdentifier/doi/10.1094/PHP-07-20-0059-Sinfo:eu-repo/semantics/altIdentifier/url/https://apsjournals.apsnet.org/doi/10.1094/PHP-07-20-0059-Sinfo:eu-repo/semantics/openAccesshttps://creativecommons.org/licenses/by-nc-sa/2.5/ar/reponame:CONICET Digital (CONICET)instname:Consejo Nacional de Investigaciones Científicas y Técnicas2025-09-03T10:10:07Zoai:ri.conicet.gov.ar:11336/167523instacron:CONICETInstitucionalhttp://ri.conicet.gov.ar/Organismo científico-tecnológicoNo correspondehttp://ri.conicet.gov.ar/oai/requestdasensio@conicet.gov.ar; lcarlino@conicet.gov.arArgentinaNo correspondeNo correspondeNo correspondeopendoar:34982025-09-03 10:10:08.166CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicasfalse |
dc.title.none.fl_str_mv |
Six species of Diaporthe associated with Phomopsis stem canker of sunflower in southern Pampean region of Argentina |
title |
Six species of Diaporthe associated with Phomopsis stem canker of sunflower in southern Pampean region of Argentina |
spellingShingle |
Six species of Diaporthe associated with Phomopsis stem canker of sunflower in southern Pampean region of Argentina Zambelli, Andres Daniel ARGENTINA DIAPORTHE PHOMOPSIS STEM CANKER SUNFLOWER |
title_short |
Six species of Diaporthe associated with Phomopsis stem canker of sunflower in southern Pampean region of Argentina |
title_full |
Six species of Diaporthe associated with Phomopsis stem canker of sunflower in southern Pampean region of Argentina |
title_fullStr |
Six species of Diaporthe associated with Phomopsis stem canker of sunflower in southern Pampean region of Argentina |
title_full_unstemmed |
Six species of Diaporthe associated with Phomopsis stem canker of sunflower in southern Pampean region of Argentina |
title_sort |
Six species of Diaporthe associated with Phomopsis stem canker of sunflower in southern Pampean region of Argentina |
dc.creator.none.fl_str_mv |
Zambelli, Andres Daniel Mancebo, María F. Bazzalo, María E. Reid, Roberto J. Sanchez, María C. Kontz, Brian J. Mathew, Febina M. |
author |
Zambelli, Andres Daniel |
author_facet |
Zambelli, Andres Daniel Mancebo, María F. Bazzalo, María E. Reid, Roberto J. Sanchez, María C. Kontz, Brian J. Mathew, Febina M. |
author_role |
author |
author2 |
Mancebo, María F. Bazzalo, María E. Reid, Roberto J. Sanchez, María C. Kontz, Brian J. Mathew, Febina M. |
author2_role |
author author author author author author |
dc.subject.none.fl_str_mv |
ARGENTINA DIAPORTHE PHOMOPSIS STEM CANKER SUNFLOWER |
topic |
ARGENTINA DIAPORTHE PHOMOPSIS STEM CANKER SUNFLOWER |
purl_subject.fl_str_mv |
https://purl.org/becyt/ford/4.1 https://purl.org/becyt/ford/4 |
dc.description.none.fl_txt_mv |
A survey of 67 commercial fields in 19 locations was conducted in the southern Pampean region of Argentina for Phomopsis stem canker of sunflower (Helianthus annuus L.) between 2014 and 2019. A total of 210 plants having typical symptoms of Phomopsis stem canker were randomly sampled, and fungal isolation was performed. Of the 187 isolates of Diaporthe that were recovered, 94% of the isolates showed morphological characteristics similar to D. helianthi, 3% to D. gulyae, 1% to D. caulivora, 1% to D. sojae, 0.5% to D. kongii, and 0.5% to D. longicolla. Following morphological characterization, the identity of the six morpho-species was confirmed by phylogenetic analyses of b-tubulin, translation elongation factor 1-a, and internal transcribed spacer gene regions. Koch’s postulates were completed for the six fungi by inoculating one susceptible sunflower hybrid with one isolate each of the six species of Diaporthe using the stem-wound inoculation method. Seven days postinoculation, significant differences in disease severity were observed between the six isolates (P < 0.0001), with D. helianthi and D. gulyae isolates causing significantly greater disease severity. To our knowledge, this is the first report of D. kongii, D. longicolla, D. caulivora, and D. sojae associated with Phomopsis stem canker of sunflower in Argentina. Fil: Zambelli, Andres Daniel. Universidad Nacional de Mar del Plata. Facultad de Ciencias Agrarias; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Mar del Plata; Argentina Fil: Mancebo, María F.. Advanta Semillas S.A.I.C.; Argentina Fil: Bazzalo, María E.. Advanta Semillas S.A.I.C.; Argentina Fil: Reid, Roberto J.. Advanta Semillas S.A.I.C.; Argentina Fil: Sanchez, María C.. Universidad Nacional de Mar del Plata. Facultad de Ciencias Agrarias; Argentina Fil: Kontz, Brian J.. South Dakota State University; Estados Unidos Fil: Mathew, Febina M.. South Dakota State University; Estados Unidos |
description |
A survey of 67 commercial fields in 19 locations was conducted in the southern Pampean region of Argentina for Phomopsis stem canker of sunflower (Helianthus annuus L.) between 2014 and 2019. A total of 210 plants having typical symptoms of Phomopsis stem canker were randomly sampled, and fungal isolation was performed. Of the 187 isolates of Diaporthe that were recovered, 94% of the isolates showed morphological characteristics similar to D. helianthi, 3% to D. gulyae, 1% to D. caulivora, 1% to D. sojae, 0.5% to D. kongii, and 0.5% to D. longicolla. Following morphological characterization, the identity of the six morpho-species was confirmed by phylogenetic analyses of b-tubulin, translation elongation factor 1-a, and internal transcribed spacer gene regions. Koch’s postulates were completed for the six fungi by inoculating one susceptible sunflower hybrid with one isolate each of the six species of Diaporthe using the stem-wound inoculation method. Seven days postinoculation, significant differences in disease severity were observed between the six isolates (P < 0.0001), with D. helianthi and D. gulyae isolates causing significantly greater disease severity. To our knowledge, this is the first report of D. kongii, D. longicolla, D. caulivora, and D. sojae associated with Phomopsis stem canker of sunflower in Argentina. |
publishDate |
2021 |
dc.date.none.fl_str_mv |
2021-01 |
dc.type.none.fl_str_mv |
info:eu-repo/semantics/article info:eu-repo/semantics/publishedVersion http://purl.org/coar/resource_type/c_6501 info:ar-repo/semantics/articulo |
format |
article |
status_str |
publishedVersion |
dc.identifier.none.fl_str_mv |
http://hdl.handle.net/11336/167523 Zambelli, Andres Daniel; Mancebo, María F.; Bazzalo, María E.; Reid, Roberto J.; Sanchez, María C.; et al.; Six species of Diaporthe associated with Phomopsis stem canker of sunflower in southern Pampean region of Argentina; American Phytopathological Society; Plant Health Progess; 22; 2; 1-2021; 136-142 1535-1025 CONICET Digital CONICET |
url |
http://hdl.handle.net/11336/167523 |
identifier_str_mv |
Zambelli, Andres Daniel; Mancebo, María F.; Bazzalo, María E.; Reid, Roberto J.; Sanchez, María C.; et al.; Six species of Diaporthe associated with Phomopsis stem canker of sunflower in southern Pampean region of Argentina; American Phytopathological Society; Plant Health Progess; 22; 2; 1-2021; 136-142 1535-1025 CONICET Digital CONICET |
dc.language.none.fl_str_mv |
eng |
language |
eng |
dc.relation.none.fl_str_mv |
info:eu-repo/semantics/altIdentifier/doi/10.1094/PHP-07-20-0059-S info:eu-repo/semantics/altIdentifier/url/https://apsjournals.apsnet.org/doi/10.1094/PHP-07-20-0059-S |
dc.rights.none.fl_str_mv |
info:eu-repo/semantics/openAccess https://creativecommons.org/licenses/by-nc-sa/2.5/ar/ |
eu_rights_str_mv |
openAccess |
rights_invalid_str_mv |
https://creativecommons.org/licenses/by-nc-sa/2.5/ar/ |
dc.format.none.fl_str_mv |
application/pdf application/pdf |
dc.publisher.none.fl_str_mv |
American Phytopathological Society |
publisher.none.fl_str_mv |
American Phytopathological Society |
dc.source.none.fl_str_mv |
reponame:CONICET Digital (CONICET) instname:Consejo Nacional de Investigaciones Científicas y Técnicas |
reponame_str |
CONICET Digital (CONICET) |
collection |
CONICET Digital (CONICET) |
instname_str |
Consejo Nacional de Investigaciones Científicas y Técnicas |
repository.name.fl_str_mv |
CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicas |
repository.mail.fl_str_mv |
dasensio@conicet.gov.ar; lcarlino@conicet.gov.ar |
_version_ |
1842270107850506240 |
score |
13.13397 |