Six species of Diaporthe associated with Phomopsis stem canker of sunflower in southern Pampean region of Argentina

Autores
Zambelli, Andres Daniel; Mancebo, María F.; Bazzalo, María E.; Reid, Roberto J.; Sanchez, María C.; Kontz, Brian J.; Mathew, Febina M.
Año de publicación
2021
Idioma
inglés
Tipo de recurso
artículo
Estado
versión publicada
Descripción
A survey of 67 commercial fields in 19 locations was conducted in the southern Pampean region of Argentina for Phomopsis stem canker of sunflower (Helianthus annuus L.) between 2014 and 2019. A total of 210 plants having typical symptoms of Phomopsis stem canker were randomly sampled, and fungal isolation was performed. Of the 187 isolates of Diaporthe that were recovered, 94% of the isolates showed morphological characteristics similar to D. helianthi, 3% to D. gulyae, 1% to D. caulivora, 1% to D. sojae, 0.5% to D. kongii, and 0.5% to D. longicolla. Following morphological characterization, the identity of the six morpho-species was confirmed by phylogenetic analyses of b-tubulin, translation elongation factor 1-a, and internal transcribed spacer gene regions. Koch’s postulates were completed for the six fungi by inoculating one susceptible sunflower hybrid with one isolate each of the six species of Diaporthe using the stem-wound inoculation method. Seven days postinoculation, significant differences in disease severity were observed between the six isolates (P < 0.0001), with D. helianthi and D. gulyae isolates causing significantly greater disease severity. To our knowledge, this is the first report of D. kongii, D. longicolla, D. caulivora, and D. sojae associated with Phomopsis stem canker of sunflower in Argentina.
Fil: Zambelli, Andres Daniel. Universidad Nacional de Mar del Plata. Facultad de Ciencias Agrarias; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Mar del Plata; Argentina
Fil: Mancebo, María F.. Advanta Semillas S.A.I.C.; Argentina
Fil: Bazzalo, María E.. Advanta Semillas S.A.I.C.; Argentina
Fil: Reid, Roberto J.. Advanta Semillas S.A.I.C.; Argentina
Fil: Sanchez, María C.. Universidad Nacional de Mar del Plata. Facultad de Ciencias Agrarias; Argentina
Fil: Kontz, Brian J.. South Dakota State University; Estados Unidos
Fil: Mathew, Febina M.. South Dakota State University; Estados Unidos
Materia
ARGENTINA
DIAPORTHE
PHOMOPSIS STEM CANKER
SUNFLOWER
Nivel de accesibilidad
acceso abierto
Condiciones de uso
https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
Repositorio
CONICET Digital (CONICET)
Institución
Consejo Nacional de Investigaciones Científicas y Técnicas
OAI Identificador
oai:ri.conicet.gov.ar:11336/167523
Acceder
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oai_identifier_str oai:ri.conicet.gov.ar:11336/167523
network_acronym_str CONICETDig
repository_id_str 3498
network_name_str CONICET Digital (CONICET)
spelling Six species of Diaporthe associated with Phomopsis stem canker of sunflower in southern Pampean region of ArgentinaZambelli, Andres DanielMancebo, María F.Bazzalo, María E.Reid, Roberto J.Sanchez, María C.Kontz, Brian J.Mathew, Febina M.ARGENTINADIAPORTHEPHOMOPSIS STEM CANKERSUNFLOWERhttps://purl.org/becyt/ford/4.1https://purl.org/becyt/ford/4A survey of 67 commercial fields in 19 locations was conducted in the southern Pampean region of Argentina for Phomopsis stem canker of sunflower (Helianthus annuus L.) between 2014 and 2019. A total of 210 plants having typical symptoms of Phomopsis stem canker were randomly sampled, and fungal isolation was performed. Of the 187 isolates of Diaporthe that were recovered, 94% of the isolates showed morphological characteristics similar to D. helianthi, 3% to D. gulyae, 1% to D. caulivora, 1% to D. sojae, 0.5% to D. kongii, and 0.5% to D. longicolla. Following morphological characterization, the identity of the six morpho-species was confirmed by phylogenetic analyses of b-tubulin, translation elongation factor 1-a, and internal transcribed spacer gene regions. Koch’s postulates were completed for the six fungi by inoculating one susceptible sunflower hybrid with one isolate each of the six species of Diaporthe using the stem-wound inoculation method. Seven days postinoculation, significant differences in disease severity were observed between the six isolates (P < 0.0001), with D. helianthi and D. gulyae isolates causing significantly greater disease severity. To our knowledge, this is the first report of D. kongii, D. longicolla, D. caulivora, and D. sojae associated with Phomopsis stem canker of sunflower in Argentina.Fil: Zambelli, Andres Daniel. Universidad Nacional de Mar del Plata. Facultad de Ciencias Agrarias; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Mar del Plata; ArgentinaFil: Mancebo, María F.. Advanta Semillas S.A.I.C.; ArgentinaFil: Bazzalo, María E.. Advanta Semillas S.A.I.C.; ArgentinaFil: Reid, Roberto J.. Advanta Semillas S.A.I.C.; ArgentinaFil: Sanchez, María C.. Universidad Nacional de Mar del Plata. Facultad de Ciencias Agrarias; ArgentinaFil: Kontz, Brian J.. South Dakota State University; Estados UnidosFil: Mathew, Febina M.. South Dakota State University; Estados UnidosAmerican Phytopathological Society2021-01info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionhttp://purl.org/coar/resource_type/c_6501info:ar-repo/semantics/articuloapplication/pdfapplication/pdfhttp://hdl.handle.net/11336/167523Zambelli, Andres Daniel; Mancebo, María F.; Bazzalo, María E.; Reid, Roberto J.; Sanchez, María C.; et al.; Six species of Diaporthe associated with Phomopsis stem canker of sunflower in southern Pampean region of Argentina; American Phytopathological Society; Plant Health Progess; 22; 2; 1-2021; 136-1421535-1025CONICET DigitalCONICETenginfo:eu-repo/semantics/altIdentifier/doi/10.1094/PHP-07-20-0059-Sinfo:eu-repo/semantics/altIdentifier/url/https://apsjournals.apsnet.org/doi/10.1094/PHP-07-20-0059-Sinfo:eu-repo/semantics/openAccesshttps://creativecommons.org/licenses/by-nc-sa/2.5/ar/reponame:CONICET Digital (CONICET)instname:Consejo Nacional de Investigaciones Científicas y Técnicas2025-09-29T10:41:55Zoai:ri.conicet.gov.ar:11336/167523instacron:CONICETInstitucionalhttp://ri.conicet.gov.ar/Organismo científico-tecnológicoNo correspondehttp://ri.conicet.gov.ar/oai/requestdasensio@conicet.gov.ar; lcarlino@conicet.gov.arArgentinaNo correspondeNo correspondeNo correspondeopendoar:34982025-09-29 10:41:56.012CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicasfalse
dc.title.none.fl_str_mv Six species of Diaporthe associated with Phomopsis stem canker of sunflower in southern Pampean region of Argentina
title Six species of Diaporthe associated with Phomopsis stem canker of sunflower in southern Pampean region of Argentina
spellingShingle Six species of Diaporthe associated with Phomopsis stem canker of sunflower in southern Pampean region of Argentina
Zambelli, Andres Daniel
ARGENTINA
DIAPORTHE
PHOMOPSIS STEM CANKER
SUNFLOWER
title_short Six species of Diaporthe associated with Phomopsis stem canker of sunflower in southern Pampean region of Argentina
title_full Six species of Diaporthe associated with Phomopsis stem canker of sunflower in southern Pampean region of Argentina
title_fullStr Six species of Diaporthe associated with Phomopsis stem canker of sunflower in southern Pampean region of Argentina
title_full_unstemmed Six species of Diaporthe associated with Phomopsis stem canker of sunflower in southern Pampean region of Argentina
title_sort Six species of Diaporthe associated with Phomopsis stem canker of sunflower in southern Pampean region of Argentina
dc.creator.none.fl_str_mv Zambelli, Andres Daniel
Mancebo, María F.
Bazzalo, María E.
Reid, Roberto J.
Sanchez, María C.
Kontz, Brian J.
Mathew, Febina M.
author Zambelli, Andres Daniel
author_facet Zambelli, Andres Daniel
Mancebo, María F.
Bazzalo, María E.
Reid, Roberto J.
Sanchez, María C.
Kontz, Brian J.
Mathew, Febina M.
author_role author
author2 Mancebo, María F.
Bazzalo, María E.
Reid, Roberto J.
Sanchez, María C.
Kontz, Brian J.
Mathew, Febina M.
author2_role author
author
author
author
author
author
dc.subject.none.fl_str_mv ARGENTINA
DIAPORTHE
PHOMOPSIS STEM CANKER
SUNFLOWER
topic ARGENTINA
DIAPORTHE
PHOMOPSIS STEM CANKER
SUNFLOWER
purl_subject.fl_str_mv https://purl.org/becyt/ford/4.1
https://purl.org/becyt/ford/4
dc.description.none.fl_txt_mv A survey of 67 commercial fields in 19 locations was conducted in the southern Pampean region of Argentina for Phomopsis stem canker of sunflower (Helianthus annuus L.) between 2014 and 2019. A total of 210 plants having typical symptoms of Phomopsis stem canker were randomly sampled, and fungal isolation was performed. Of the 187 isolates of Diaporthe that were recovered, 94% of the isolates showed morphological characteristics similar to D. helianthi, 3% to D. gulyae, 1% to D. caulivora, 1% to D. sojae, 0.5% to D. kongii, and 0.5% to D. longicolla. Following morphological characterization, the identity of the six morpho-species was confirmed by phylogenetic analyses of b-tubulin, translation elongation factor 1-a, and internal transcribed spacer gene regions. Koch’s postulates were completed for the six fungi by inoculating one susceptible sunflower hybrid with one isolate each of the six species of Diaporthe using the stem-wound inoculation method. Seven days postinoculation, significant differences in disease severity were observed between the six isolates (P < 0.0001), with D. helianthi and D. gulyae isolates causing significantly greater disease severity. To our knowledge, this is the first report of D. kongii, D. longicolla, D. caulivora, and D. sojae associated with Phomopsis stem canker of sunflower in Argentina.
Fil: Zambelli, Andres Daniel. Universidad Nacional de Mar del Plata. Facultad de Ciencias Agrarias; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Mar del Plata; Argentina
Fil: Mancebo, María F.. Advanta Semillas S.A.I.C.; Argentina
Fil: Bazzalo, María E.. Advanta Semillas S.A.I.C.; Argentina
Fil: Reid, Roberto J.. Advanta Semillas S.A.I.C.; Argentina
Fil: Sanchez, María C.. Universidad Nacional de Mar del Plata. Facultad de Ciencias Agrarias; Argentina
Fil: Kontz, Brian J.. South Dakota State University; Estados Unidos
Fil: Mathew, Febina M.. South Dakota State University; Estados Unidos
description A survey of 67 commercial fields in 19 locations was conducted in the southern Pampean region of Argentina for Phomopsis stem canker of sunflower (Helianthus annuus L.) between 2014 and 2019. A total of 210 plants having typical symptoms of Phomopsis stem canker were randomly sampled, and fungal isolation was performed. Of the 187 isolates of Diaporthe that were recovered, 94% of the isolates showed morphological characteristics similar to D. helianthi, 3% to D. gulyae, 1% to D. caulivora, 1% to D. sojae, 0.5% to D. kongii, and 0.5% to D. longicolla. Following morphological characterization, the identity of the six morpho-species was confirmed by phylogenetic analyses of b-tubulin, translation elongation factor 1-a, and internal transcribed spacer gene regions. Koch’s postulates were completed for the six fungi by inoculating one susceptible sunflower hybrid with one isolate each of the six species of Diaporthe using the stem-wound inoculation method. Seven days postinoculation, significant differences in disease severity were observed between the six isolates (P < 0.0001), with D. helianthi and D. gulyae isolates causing significantly greater disease severity. To our knowledge, this is the first report of D. kongii, D. longicolla, D. caulivora, and D. sojae associated with Phomopsis stem canker of sunflower in Argentina.
publishDate 2021
dc.date.none.fl_str_mv 2021-01
dc.type.none.fl_str_mv info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
http://purl.org/coar/resource_type/c_6501
info:ar-repo/semantics/articulo
format article
status_str publishedVersion
dc.identifier.none.fl_str_mv http://hdl.handle.net/11336/167523
Zambelli, Andres Daniel; Mancebo, María F.; Bazzalo, María E.; Reid, Roberto J.; Sanchez, María C.; et al.; Six species of Diaporthe associated with Phomopsis stem canker of sunflower in southern Pampean region of Argentina; American Phytopathological Society; Plant Health Progess; 22; 2; 1-2021; 136-142
1535-1025
CONICET Digital
CONICET
url http://hdl.handle.net/11336/167523
identifier_str_mv Zambelli, Andres Daniel; Mancebo, María F.; Bazzalo, María E.; Reid, Roberto J.; Sanchez, María C.; et al.; Six species of Diaporthe associated with Phomopsis stem canker of sunflower in southern Pampean region of Argentina; American Phytopathological Society; Plant Health Progess; 22; 2; 1-2021; 136-142
1535-1025
CONICET Digital
CONICET
dc.language.none.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv info:eu-repo/semantics/altIdentifier/doi/10.1094/PHP-07-20-0059-S
info:eu-repo/semantics/altIdentifier/url/https://apsjournals.apsnet.org/doi/10.1094/PHP-07-20-0059-S
dc.rights.none.fl_str_mv info:eu-repo/semantics/openAccess
https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
eu_rights_str_mv openAccess
rights_invalid_str_mv https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
dc.format.none.fl_str_mv application/pdf
application/pdf
dc.publisher.none.fl_str_mv American Phytopathological Society
publisher.none.fl_str_mv American Phytopathological Society
dc.source.none.fl_str_mv reponame:CONICET Digital (CONICET)
instname:Consejo Nacional de Investigaciones Científicas y Técnicas
reponame_str CONICET Digital (CONICET)
collection CONICET Digital (CONICET)
instname_str Consejo Nacional de Investigaciones Científicas y Técnicas
repository.name.fl_str_mv CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicas
repository.mail.fl_str_mv dasensio@conicet.gov.ar; lcarlino@conicet.gov.ar
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score 13.069144

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