A cheap and quick method for DNA-based sexing of birds

Autores
Quintana, Flavio Roberto; Lopez, Gabriela Carina; Somoza, Gustavo Manuel
Año de publicación
2008
Idioma
inglés
Tipo de recurso
artículo
Estado
versión publicada
Descripción
Although the collection of air-dried blood samples on filter paper has been used for field work as a routine, it has required the use of special and expensive types of paper. In the present manuscript the use of common filter paper to collect and store air-dried bird blood samples is validated for its use in DNA based sexing techniques with a very simple method avoiding DNA purification for PCR. This method not only simplifies the laboratory work but also does not add any additional cost for DNA-based sexing techniques. The method was tested and successfully employed for sex determination by molecular techniques on blood samples taken on common filter paper during different field trips from four seabird species.
Fil: Quintana, Flavio Roberto. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Centro Nacional Patagónico. Instituto de Biología de Organismos Marinos; Argentina
Fil: Lopez, Gabriela Carina. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - La Plata. Instituto de Investigaciones Biotecnológicas. Instituto de Investigaciones Biotecnológicas "Dr. Raúl Alfonsín" (sede Chascomús). Universidad Nacional de San Martín. Instituto de Investigaciones Biotecnológicas. Instituto de Investigaciones Biotecnológicas "Dr. Raúl Alfonsín" (sede Chascomús); Argentina
Fil: Somoza, Gustavo Manuel. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - La Plata. Instituto de Investigaciones Biotecnológicas. Instituto de Investigaciones Biotecnológicas "Dr. Raúl Alfonsín" (sede Chascomús). Universidad Nacional de San Martín. Instituto de Investigaciones Biotecnológicas. Instituto de Investigaciones Biotecnológicas "Dr. Raúl Alfonsín" (sede Chascomús); Argentina
Materia
CHD1-Z
CHD1-W
Field samples
Molecular sexing
PCR
Seabirds
Nivel de accesibilidad
acceso abierto
Condiciones de uso
https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
Repositorio
CONICET Digital (CONICET)
Institución
Consejo Nacional de Investigaciones Científicas y Técnicas
OAI Identificador
oai:ri.conicet.gov.ar:11336/101301
Acceder
id CONICETDig_1ce6a6bec1020d5063882f9c347c19ff
oai_identifier_str oai:ri.conicet.gov.ar:11336/101301
network_acronym_str CONICETDig
repository_id_str 3498
network_name_str CONICET Digital (CONICET)
spelling A cheap and quick method for DNA-based sexing of birdsQuintana, Flavio RobertoLopez, Gabriela CarinaSomoza, Gustavo ManuelCHD1-ZCHD1-WField samplesMolecular sexingPCRSeabirdshttps://purl.org/becyt/ford/1.6https://purl.org/becyt/ford/1Although the collection of air-dried blood samples on filter paper has been used for field work as a routine, it has required the use of special and expensive types of paper. In the present manuscript the use of common filter paper to collect and store air-dried bird blood samples is validated for its use in DNA based sexing techniques with a very simple method avoiding DNA purification for PCR. This method not only simplifies the laboratory work but also does not add any additional cost for DNA-based sexing techniques. The method was tested and successfully employed for sex determination by molecular techniques on blood samples taken on common filter paper during different field trips from four seabird species.Fil: Quintana, Flavio Roberto. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Centro Nacional Patagónico. Instituto de Biología de Organismos Marinos; ArgentinaFil: Lopez, Gabriela Carina. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - La Plata. Instituto de Investigaciones Biotecnológicas. Instituto de Investigaciones Biotecnológicas "Dr. Raúl Alfonsín" (sede Chascomús). Universidad Nacional de San Martín. Instituto de Investigaciones Biotecnológicas. Instituto de Investigaciones Biotecnológicas "Dr. Raúl Alfonsín" (sede Chascomús); ArgentinaFil: Somoza, Gustavo Manuel. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - La Plata. Instituto de Investigaciones Biotecnológicas. Instituto de Investigaciones Biotecnológicas "Dr. Raúl Alfonsín" (sede Chascomús). Universidad Nacional de San Martín. Instituto de Investigaciones Biotecnológicas. Instituto de Investigaciones Biotecnológicas "Dr. Raúl Alfonsín" (sede Chascomús); ArgentinaWaterbird Society2008-09info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionhttp://purl.org/coar/resource_type/c_6501info:ar-repo/semantics/articuloapplication/pdfapplication/pdfapplication/pdfapplication/pdfhttp://hdl.handle.net/11336/101301Quintana, Flavio Roberto; Lopez, Gabriela Carina; Somoza, Gustavo Manuel; A cheap and quick method for DNA-based sexing of birds; Waterbird Society; Waterbirds; 31; 3; 9-2008; 485-4881524-46951938-5390CONICET DigitalCONICETenginfo:eu-repo/semantics/altIdentifier/url/https://www.jstor.org/stable/25148359?seq=1info:eu-repo/semantics/openAccesshttps://creativecommons.org/licenses/by-nc-sa/2.5/ar/reponame:CONICET Digital (CONICET)instname:Consejo Nacional de Investigaciones Científicas y Técnicas2025-10-15T15:11:01Zoai:ri.conicet.gov.ar:11336/101301instacron:CONICETInstitucionalhttp://ri.conicet.gov.ar/Organismo científico-tecnológicoNo correspondehttp://ri.conicet.gov.ar/oai/requestdasensio@conicet.gov.ar; lcarlino@conicet.gov.arArgentinaNo correspondeNo correspondeNo correspondeopendoar:34982025-10-15 15:11:01.885CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicasfalse
dc.title.none.fl_str_mv A cheap and quick method for DNA-based sexing of birds
title A cheap and quick method for DNA-based sexing of birds
spellingShingle A cheap and quick method for DNA-based sexing of birds
Quintana, Flavio Roberto
CHD1-Z
CHD1-W
Field samples
Molecular sexing
PCR
Seabirds
title_short A cheap and quick method for DNA-based sexing of birds
title_full A cheap and quick method for DNA-based sexing of birds
title_fullStr A cheap and quick method for DNA-based sexing of birds
title_full_unstemmed A cheap and quick method for DNA-based sexing of birds
title_sort A cheap and quick method for DNA-based sexing of birds
dc.creator.none.fl_str_mv Quintana, Flavio Roberto
Lopez, Gabriela Carina
Somoza, Gustavo Manuel
author Quintana, Flavio Roberto
author_facet Quintana, Flavio Roberto
Lopez, Gabriela Carina
Somoza, Gustavo Manuel
author_role author
author2 Lopez, Gabriela Carina
Somoza, Gustavo Manuel
author2_role author
author
dc.subject.none.fl_str_mv CHD1-Z
CHD1-W
Field samples
Molecular sexing
PCR
Seabirds
topic CHD1-Z
CHD1-W
Field samples
Molecular sexing
PCR
Seabirds
purl_subject.fl_str_mv https://purl.org/becyt/ford/1.6
https://purl.org/becyt/ford/1
dc.description.none.fl_txt_mv Although the collection of air-dried blood samples on filter paper has been used for field work as a routine, it has required the use of special and expensive types of paper. In the present manuscript the use of common filter paper to collect and store air-dried bird blood samples is validated for its use in DNA based sexing techniques with a very simple method avoiding DNA purification for PCR. This method not only simplifies the laboratory work but also does not add any additional cost for DNA-based sexing techniques. The method was tested and successfully employed for sex determination by molecular techniques on blood samples taken on common filter paper during different field trips from four seabird species.
Fil: Quintana, Flavio Roberto. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Centro Nacional Patagónico. Instituto de Biología de Organismos Marinos; Argentina
Fil: Lopez, Gabriela Carina. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - La Plata. Instituto de Investigaciones Biotecnológicas. Instituto de Investigaciones Biotecnológicas "Dr. Raúl Alfonsín" (sede Chascomús). Universidad Nacional de San Martín. Instituto de Investigaciones Biotecnológicas. Instituto de Investigaciones Biotecnológicas "Dr. Raúl Alfonsín" (sede Chascomús); Argentina
Fil: Somoza, Gustavo Manuel. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - La Plata. Instituto de Investigaciones Biotecnológicas. Instituto de Investigaciones Biotecnológicas "Dr. Raúl Alfonsín" (sede Chascomús). Universidad Nacional de San Martín. Instituto de Investigaciones Biotecnológicas. Instituto de Investigaciones Biotecnológicas "Dr. Raúl Alfonsín" (sede Chascomús); Argentina
description Although the collection of air-dried blood samples on filter paper has been used for field work as a routine, it has required the use of special and expensive types of paper. In the present manuscript the use of common filter paper to collect and store air-dried bird blood samples is validated for its use in DNA based sexing techniques with a very simple method avoiding DNA purification for PCR. This method not only simplifies the laboratory work but also does not add any additional cost for DNA-based sexing techniques. The method was tested and successfully employed for sex determination by molecular techniques on blood samples taken on common filter paper during different field trips from four seabird species.
publishDate 2008
dc.date.none.fl_str_mv 2008-09
dc.type.none.fl_str_mv info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
http://purl.org/coar/resource_type/c_6501
info:ar-repo/semantics/articulo
format article
status_str publishedVersion
dc.identifier.none.fl_str_mv http://hdl.handle.net/11336/101301
Quintana, Flavio Roberto; Lopez, Gabriela Carina; Somoza, Gustavo Manuel; A cheap and quick method for DNA-based sexing of birds; Waterbird Society; Waterbirds; 31; 3; 9-2008; 485-488
1524-4695
1938-5390
CONICET Digital
CONICET
url http://hdl.handle.net/11336/101301
identifier_str_mv Quintana, Flavio Roberto; Lopez, Gabriela Carina; Somoza, Gustavo Manuel; A cheap and quick method for DNA-based sexing of birds; Waterbird Society; Waterbirds; 31; 3; 9-2008; 485-488
1524-4695
1938-5390
CONICET Digital
CONICET
dc.language.none.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv info:eu-repo/semantics/altIdentifier/url/https://www.jstor.org/stable/25148359?seq=1
dc.rights.none.fl_str_mv info:eu-repo/semantics/openAccess
https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
eu_rights_str_mv openAccess
rights_invalid_str_mv https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
dc.format.none.fl_str_mv application/pdf
application/pdf
application/pdf
application/pdf
dc.publisher.none.fl_str_mv Waterbird Society
publisher.none.fl_str_mv Waterbird Society
dc.source.none.fl_str_mv reponame:CONICET Digital (CONICET)
instname:Consejo Nacional de Investigaciones Científicas y Técnicas
reponame_str CONICET Digital (CONICET)
collection CONICET Digital (CONICET)
instname_str Consejo Nacional de Investigaciones Científicas y Técnicas
repository.name.fl_str_mv CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicas
repository.mail.fl_str_mv dasensio@conicet.gov.ar; lcarlino@conicet.gov.ar
_version_ 1846083258314391552
score 13.22299

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