Gender-Dependent Effects of Early Maternal Separation and Variable Chronic Stress on Vasopressinergic Activity and Glucocorticoid Receptor Expression in Adult Rats

Autores
Renard, Georgina Maria; Rivarola, María Angélica; Suarez, Marta Magdalena
Año de publicación
2010
Idioma
inglés
Tipo de recurso
artículo
Estado
versión publicada
Descripción
The aim of the present study was to investigate the influence of early maternal separation on Fos, arginine vasopressin (AVP) and glucocorticoid receptor (GR) expression in the medial parvocellular portion of the paraventricular hypothalamic nucleus (PaMP), and GR expression in the hippocampus of adult male and female rats subjected to variable chronic stress (VCS). Male and female Wistar rats were isolated 4.5 h daily, during the first 3 weeks of life. At 48 days of age, the rats were exposed to VCS. Nonmaternally separated (NMS) females had a higher number of activated AVP neurons than NMS male rats. Maternally separated (MS) females subjected to VCS also showed a higher number of Fos/AVP double-labeled neurons than males with the same treatment. Males and females subjected to early maternal separation and VCS, compared with the MS animals, showed a decrease in the expression of GR in the PaMP. As regards GR expression in the hippocampus, MS animals subjected to VCS as adults, both males and females, showed an increase in GR expression in the subfields CA1, CA2 and CA3. The increase in AVP-immunoreactive neurons coexpressing Fos in response to stress in females exposed to early maternal separation suggests that perhaps early life stress results in a more reactive neuroendocrine stress response in females. Furthermore, our results demonstrate that the different anatomical levels of the hypothalamic-pituitary-adrenal axis have different roles related to its stress response and support the evidence of regional specificity in GR regulation.
Fil: Renard, Georgina Maria. Universidad Nacional de Córdoba. Facultad de Cs.exactas Físicas y Naturales. Departamento de Fisiología Animal; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Córdoba; Argentina
Fil: Rivarola, María Angélica. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Córdoba. Instituto de Investigaciones en Ciencias de la Salud. Universidad Nacional de Córdoba. Instituto de Investigaciones en Ciencias de la Salud; Argentina
Fil: Suarez, Marta Magdalena. Universidad Nacional de Córdoba. Facultad de Cs.exactas Físicas y Naturales. Departamento de Fisiología Animal; Argentina
Materia
ARGININE VASOPRESSIN
FOS
GLUCOCORTICOID RECEPTORS
HIPPOCAMPUS
MATERNAL SEPARATION
SEXUAL DIMORPHISM
VARIABLE CHRONIC STRESS
Nivel de accesibilidad
acceso abierto
Condiciones de uso
https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
Repositorio
CONICET Digital (CONICET)
Institución
Consejo Nacional de Investigaciones Científicas y Técnicas
OAI Identificador
oai:ri.conicet.gov.ar:11336/189563
Acceder
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oai_identifier_str oai:ri.conicet.gov.ar:11336/189563
network_acronym_str CONICETDig
repository_id_str 3498
network_name_str CONICET Digital (CONICET)
spelling Gender-Dependent Effects of Early Maternal Separation and Variable Chronic Stress on Vasopressinergic Activity and Glucocorticoid Receptor Expression in Adult RatsRenard, Georgina MariaRivarola, María AngélicaSuarez, Marta MagdalenaARGININE VASOPRESSINFOSGLUCOCORTICOID RECEPTORSHIPPOCAMPUSMATERNAL SEPARATIONSEXUAL DIMORPHISMVARIABLE CHRONIC STRESShttps://purl.org/becyt/ford/1.6https://purl.org/becyt/ford/1The aim of the present study was to investigate the influence of early maternal separation on Fos, arginine vasopressin (AVP) and glucocorticoid receptor (GR) expression in the medial parvocellular portion of the paraventricular hypothalamic nucleus (PaMP), and GR expression in the hippocampus of adult male and female rats subjected to variable chronic stress (VCS). Male and female Wistar rats were isolated 4.5 h daily, during the first 3 weeks of life. At 48 days of age, the rats were exposed to VCS. Nonmaternally separated (NMS) females had a higher number of activated AVP neurons than NMS male rats. Maternally separated (MS) females subjected to VCS also showed a higher number of Fos/AVP double-labeled neurons than males with the same treatment. Males and females subjected to early maternal separation and VCS, compared with the MS animals, showed a decrease in the expression of GR in the PaMP. As regards GR expression in the hippocampus, MS animals subjected to VCS as adults, both males and females, showed an increase in GR expression in the subfields CA1, CA2 and CA3. The increase in AVP-immunoreactive neurons coexpressing Fos in response to stress in females exposed to early maternal separation suggests that perhaps early life stress results in a more reactive neuroendocrine stress response in females. Furthermore, our results demonstrate that the different anatomical levels of the hypothalamic-pituitary-adrenal axis have different roles related to its stress response and support the evidence of regional specificity in GR regulation.Fil: Renard, Georgina Maria. Universidad Nacional de Córdoba. Facultad de Cs.exactas Físicas y Naturales. Departamento de Fisiología Animal; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Córdoba; ArgentinaFil: Rivarola, María Angélica. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Córdoba. Instituto de Investigaciones en Ciencias de la Salud. Universidad Nacional de Córdoba. Instituto de Investigaciones en Ciencias de la Salud; ArgentinaFil: Suarez, Marta Magdalena. Universidad Nacional de Córdoba. Facultad de Cs.exactas Físicas y Naturales. Departamento de Fisiología Animal; ArgentinaKarger2010-03-25info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionhttp://purl.org/coar/resource_type/c_6501info:ar-repo/semantics/articuloapplication/pdfapplication/pdfapplication/pdfapplication/pdfhttp://hdl.handle.net/11336/189563Renard, Georgina Maria; Rivarola, María Angélica; Suarez, Marta Magdalena; Gender-Dependent Effects of Early Maternal Separation and Variable Chronic Stress on Vasopressinergic Activity and Glucocorticoid Receptor Expression in Adult Rats; Karger; Developmental Neuroscience; 32; 1; 25-3-2010; 71-800378-58661421-9859CONICET DigitalCONICETenginfo:eu-repo/semantics/altIdentifier/doi/10.1159%2F000280102info:eu-repo/semantics/openAccesshttps://creativecommons.org/licenses/by-nc-sa/2.5/ar/reponame:CONICET Digital (CONICET)instname:Consejo Nacional de Investigaciones Científicas y Técnicas2025-10-22T12:13:26Zoai:ri.conicet.gov.ar:11336/189563instacron:CONICETInstitucionalhttp://ri.conicet.gov.ar/Organismo científico-tecnológicoNo correspondehttp://ri.conicet.gov.ar/oai/requestdasensio@conicet.gov.ar; lcarlino@conicet.gov.arArgentinaNo correspondeNo correspondeNo correspondeopendoar:34982025-10-22 12:13:26.566CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicasfalse
dc.title.none.fl_str_mv Gender-Dependent Effects of Early Maternal Separation and Variable Chronic Stress on Vasopressinergic Activity and Glucocorticoid Receptor Expression in Adult Rats
title Gender-Dependent Effects of Early Maternal Separation and Variable Chronic Stress on Vasopressinergic Activity and Glucocorticoid Receptor Expression in Adult Rats
spellingShingle Gender-Dependent Effects of Early Maternal Separation and Variable Chronic Stress on Vasopressinergic Activity and Glucocorticoid Receptor Expression in Adult Rats
Renard, Georgina Maria
ARGININE VASOPRESSIN
FOS
GLUCOCORTICOID RECEPTORS
HIPPOCAMPUS
MATERNAL SEPARATION
SEXUAL DIMORPHISM
VARIABLE CHRONIC STRESS
title_short Gender-Dependent Effects of Early Maternal Separation and Variable Chronic Stress on Vasopressinergic Activity and Glucocorticoid Receptor Expression in Adult Rats
title_full Gender-Dependent Effects of Early Maternal Separation and Variable Chronic Stress on Vasopressinergic Activity and Glucocorticoid Receptor Expression in Adult Rats
title_fullStr Gender-Dependent Effects of Early Maternal Separation and Variable Chronic Stress on Vasopressinergic Activity and Glucocorticoid Receptor Expression in Adult Rats
title_full_unstemmed Gender-Dependent Effects of Early Maternal Separation and Variable Chronic Stress on Vasopressinergic Activity and Glucocorticoid Receptor Expression in Adult Rats
title_sort Gender-Dependent Effects of Early Maternal Separation and Variable Chronic Stress on Vasopressinergic Activity and Glucocorticoid Receptor Expression in Adult Rats
dc.creator.none.fl_str_mv Renard, Georgina Maria
Rivarola, María Angélica
Suarez, Marta Magdalena
author Renard, Georgina Maria
author_facet Renard, Georgina Maria
Rivarola, María Angélica
Suarez, Marta Magdalena
author_role author
author2 Rivarola, María Angélica
Suarez, Marta Magdalena
author2_role author
author
dc.subject.none.fl_str_mv ARGININE VASOPRESSIN
FOS
GLUCOCORTICOID RECEPTORS
HIPPOCAMPUS
MATERNAL SEPARATION
SEXUAL DIMORPHISM
VARIABLE CHRONIC STRESS
topic ARGININE VASOPRESSIN
FOS
GLUCOCORTICOID RECEPTORS
HIPPOCAMPUS
MATERNAL SEPARATION
SEXUAL DIMORPHISM
VARIABLE CHRONIC STRESS
purl_subject.fl_str_mv https://purl.org/becyt/ford/1.6
https://purl.org/becyt/ford/1
dc.description.none.fl_txt_mv The aim of the present study was to investigate the influence of early maternal separation on Fos, arginine vasopressin (AVP) and glucocorticoid receptor (GR) expression in the medial parvocellular portion of the paraventricular hypothalamic nucleus (PaMP), and GR expression in the hippocampus of adult male and female rats subjected to variable chronic stress (VCS). Male and female Wistar rats were isolated 4.5 h daily, during the first 3 weeks of life. At 48 days of age, the rats were exposed to VCS. Nonmaternally separated (NMS) females had a higher number of activated AVP neurons than NMS male rats. Maternally separated (MS) females subjected to VCS also showed a higher number of Fos/AVP double-labeled neurons than males with the same treatment. Males and females subjected to early maternal separation and VCS, compared with the MS animals, showed a decrease in the expression of GR in the PaMP. As regards GR expression in the hippocampus, MS animals subjected to VCS as adults, both males and females, showed an increase in GR expression in the subfields CA1, CA2 and CA3. The increase in AVP-immunoreactive neurons coexpressing Fos in response to stress in females exposed to early maternal separation suggests that perhaps early life stress results in a more reactive neuroendocrine stress response in females. Furthermore, our results demonstrate that the different anatomical levels of the hypothalamic-pituitary-adrenal axis have different roles related to its stress response and support the evidence of regional specificity in GR regulation.
Fil: Renard, Georgina Maria. Universidad Nacional de Córdoba. Facultad de Cs.exactas Físicas y Naturales. Departamento de Fisiología Animal; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Córdoba; Argentina
Fil: Rivarola, María Angélica. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Córdoba. Instituto de Investigaciones en Ciencias de la Salud. Universidad Nacional de Córdoba. Instituto de Investigaciones en Ciencias de la Salud; Argentina
Fil: Suarez, Marta Magdalena. Universidad Nacional de Córdoba. Facultad de Cs.exactas Físicas y Naturales. Departamento de Fisiología Animal; Argentina
description The aim of the present study was to investigate the influence of early maternal separation on Fos, arginine vasopressin (AVP) and glucocorticoid receptor (GR) expression in the medial parvocellular portion of the paraventricular hypothalamic nucleus (PaMP), and GR expression in the hippocampus of adult male and female rats subjected to variable chronic stress (VCS). Male and female Wistar rats were isolated 4.5 h daily, during the first 3 weeks of life. At 48 days of age, the rats were exposed to VCS. Nonmaternally separated (NMS) females had a higher number of activated AVP neurons than NMS male rats. Maternally separated (MS) females subjected to VCS also showed a higher number of Fos/AVP double-labeled neurons than males with the same treatment. Males and females subjected to early maternal separation and VCS, compared with the MS animals, showed a decrease in the expression of GR in the PaMP. As regards GR expression in the hippocampus, MS animals subjected to VCS as adults, both males and females, showed an increase in GR expression in the subfields CA1, CA2 and CA3. The increase in AVP-immunoreactive neurons coexpressing Fos in response to stress in females exposed to early maternal separation suggests that perhaps early life stress results in a more reactive neuroendocrine stress response in females. Furthermore, our results demonstrate that the different anatomical levels of the hypothalamic-pituitary-adrenal axis have different roles related to its stress response and support the evidence of regional specificity in GR regulation.
publishDate 2010
dc.date.none.fl_str_mv 2010-03-25
dc.type.none.fl_str_mv info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
http://purl.org/coar/resource_type/c_6501
info:ar-repo/semantics/articulo
format article
status_str publishedVersion
dc.identifier.none.fl_str_mv http://hdl.handle.net/11336/189563
Renard, Georgina Maria; Rivarola, María Angélica; Suarez, Marta Magdalena; Gender-Dependent Effects of Early Maternal Separation and Variable Chronic Stress on Vasopressinergic Activity and Glucocorticoid Receptor Expression in Adult Rats; Karger; Developmental Neuroscience; 32; 1; 25-3-2010; 71-80
0378-5866
1421-9859
CONICET Digital
CONICET
url http://hdl.handle.net/11336/189563
identifier_str_mv Renard, Georgina Maria; Rivarola, María Angélica; Suarez, Marta Magdalena; Gender-Dependent Effects of Early Maternal Separation and Variable Chronic Stress on Vasopressinergic Activity and Glucocorticoid Receptor Expression in Adult Rats; Karger; Developmental Neuroscience; 32; 1; 25-3-2010; 71-80
0378-5866
1421-9859
CONICET Digital
CONICET
dc.language.none.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv info:eu-repo/semantics/altIdentifier/doi/10.1159%2F000280102
dc.rights.none.fl_str_mv info:eu-repo/semantics/openAccess
https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
eu_rights_str_mv openAccess
rights_invalid_str_mv https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
dc.format.none.fl_str_mv application/pdf
application/pdf
application/pdf
application/pdf
dc.publisher.none.fl_str_mv Karger
publisher.none.fl_str_mv Karger
dc.source.none.fl_str_mv reponame:CONICET Digital (CONICET)
instname:Consejo Nacional de Investigaciones Científicas y Técnicas
reponame_str CONICET Digital (CONICET)
collection CONICET Digital (CONICET)
instname_str Consejo Nacional de Investigaciones Científicas y Técnicas
repository.name.fl_str_mv CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicas
repository.mail.fl_str_mv dasensio@conicet.gov.ar; lcarlino@conicet.gov.ar
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score 12.982451

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