An inter-laboratory study of DNA-based identity, parentage and species testing in animal forensic genetics
- Autores
- Kanthaswamy, Sreetharan; Brendel, Torsten; Cancela, Luis; Andrade de Oliveira, Denise A.; Brenig, Bertram; Cons, Carmen; Crespi, Julian Alejandro; Dajbychová, Markéta; Feldl, Andreas; Itoh, Tomohito; Landi, Vincenzo; Martinez, Amparo; Natonek-Wisniewska, Malgorzata; Oldt, Robert F.; Radko, Anna; Ramírez, Oscar; Rodellar, Clementina; Ruiz Girón, Manuel; Schikorski, David; Turba, María Elena; Giovambattista, Guillermo
- Año de publicación
- 2021
- Idioma
- inglés
- Tipo de recurso
- artículo
- Estado
- versión publicada
- Descripción
- The probative value of animal forensic genetic evidence relies on laboratory accuracy and reliability. Inter-laboratory comparisons allow laboratories to evaluate their performance on specific tests and analyses and to continue to monitor their output. The International Society for Animal Genetics (ISAG) administered animal forensic comparison tests (AFCTs) in 2016 and 2018 to assess the limitations and capabilities of laboratories offering forensic identification, parentage and species determination services. The AFCTs revealed that analyses of low DNA template concentrations (≤300 pg/µL) constitute a significant challenge that has prevented many laboratories from reporting correct identification and parentage results. Moreover, a lack of familiarity with species testing protocols, interpretation guidelines and representative databases prevented over a quarter of the participating laboratories from submitting correct species determination results. Several laboratories showed improvement in their genotyping accuracy over time. However, the use of forensically validated standards, such as a standard forensic short tandem repeat (STR) kit, preferably with an allelic ladder, and stricter guidelines for STR typing, may have prevented some common issues from occurring, such as genotyping inaccuracies, missing data, elevated stutter products and loading errors. The AFCTs underscore the importance of conducting routine forensic comparison tests to allow laboratories to compare results from each other. Laboratories should keep improving their scientific and technical capabilities and continuously evaluate their personnel’s proficiency in critical techniques such as low copy number (LCN) analysis and species testing. Although this is the first time that the ISAG has conducted comparison tests for forensic testing, findings from these AFCTs may serve as the foundation for continuous improvements of the overall quality of animal forensic genetic testing.
Fil: Kanthaswamy, Sreetharan. Arizona State University; Estados Unidos. University of California at Davis; Estados Unidos
Fil: Brendel, Torsten. Eurofins Genomics Europe Applied Genomics GmbH; Alemania
Fil: Cancela, Luis. Identitas; Uruguay
Fil: Andrade de Oliveira, Denise A.. Universidade Federal de Minas Gerais; Brasil
Fil: Brenig, Bertram. Universität Göttingen; Alemania
Fil: Cons, Carmen. Universidad de Zaragoza. Instituto Agroalimentario de Aragon; España
Fil: Crespi, Julian Alejandro. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico CONICET- La Plata. Instituto de Genética Veterinaria "Ing. Fernando Noel Dulout". Universidad Nacional de La Plata. Facultad de Ciencias Veterinarias. Instituto de Genética Veterinaria; Argentina
Fil: Dajbychová, Markéta. Genomia s.r.o.; República Checa
Fil: Feldl, Andreas. Eurofins Genomics Europe Applied Genomics GmbH; Alemania
Fil: Itoh, Tomohito. Livestock Improvement Association of Japan; Japón
Fil: Landi, Vincenzo. Università degli Studi di Bari; Italia
Fil: Martinez, Amparo. Universidad de Córdoba; España
Fil: Natonek-Wisniewska, Malgorzata. National Research Institute of Animal Production; Polonia
Fil: Oldt, Robert F.. Arizona State University; Estados Unidos. University of California at Davis; Estados Unidos
Fil: Radko, Anna. Vetgenomics S.L., Barcelona; España
Fil: Ramírez, Oscar. Vetgenomics S.L., Barcelona; España
Fil: Rodellar, Clementina. Universidad de Zaragoza. Instituto Agroalimentario de Aragon; España
Fil: Ruiz Girón, Manuel. Hispalis Biolab S.L.U., Sevilla; España
Fil: Schikorski, David. Laboratoire LABOFARM-GENINDEXE; Francia
Fil: Turba, María Elena. Genefast SRL ; Italia
Fil: Giovambattista, Guillermo. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico CONICET- La Plata. Instituto de Genética Veterinaria "Ing. Fernando Noel Dulout". Universidad Nacional de La Plata. Facultad de Ciencias Veterinarias. Instituto de Genética Veterinaria; Argentina - Materia
-
COMPARISON TEST
FORENSIC DNA ANALYSIS
FORENSIC GENETICS
FORENSIC SCIENCES
INDIVIDUAL IDENTIFICATION
PARENTAGE TEST
SPECIES TEST - Nivel de accesibilidad
- acceso abierto
- Condiciones de uso
- https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
- Repositorio
.jpg)
- Institución
- Consejo Nacional de Investigaciones Científicas y Técnicas
- OAI Identificador
- oai:ri.conicet.gov.ar:11336/158180
Ver los metadatos del registro completo
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An inter-laboratory study of DNA-based identity, parentage and species testing in animal forensic geneticsKanthaswamy, SreetharanBrendel, TorstenCancela, LuisAndrade de Oliveira, Denise A.Brenig, BertramCons, CarmenCrespi, Julian AlejandroDajbychová, MarkétaFeldl, AndreasItoh, TomohitoLandi, VincenzoMartinez, AmparoNatonek-Wisniewska, MalgorzataOldt, Robert F.Radko, AnnaRamírez, OscarRodellar, ClementinaRuiz Girón, ManuelSchikorski, DavidTurba, María ElenaGiovambattista, GuillermoCOMPARISON TESTFORENSIC DNA ANALYSISFORENSIC GENETICSFORENSIC SCIENCESINDIVIDUAL IDENTIFICATIONPARENTAGE TESTSPECIES TESThttps://purl.org/becyt/ford/4.3https://purl.org/becyt/ford/4The probative value of animal forensic genetic evidence relies on laboratory accuracy and reliability. Inter-laboratory comparisons allow laboratories to evaluate their performance on specific tests and analyses and to continue to monitor their output. The International Society for Animal Genetics (ISAG) administered animal forensic comparison tests (AFCTs) in 2016 and 2018 to assess the limitations and capabilities of laboratories offering forensic identification, parentage and species determination services. The AFCTs revealed that analyses of low DNA template concentrations (≤300 pg/µL) constitute a significant challenge that has prevented many laboratories from reporting correct identification and parentage results. Moreover, a lack of familiarity with species testing protocols, interpretation guidelines and representative databases prevented over a quarter of the participating laboratories from submitting correct species determination results. Several laboratories showed improvement in their genotyping accuracy over time. However, the use of forensically validated standards, such as a standard forensic short tandem repeat (STR) kit, preferably with an allelic ladder, and stricter guidelines for STR typing, may have prevented some common issues from occurring, such as genotyping inaccuracies, missing data, elevated stutter products and loading errors. The AFCTs underscore the importance of conducting routine forensic comparison tests to allow laboratories to compare results from each other. Laboratories should keep improving their scientific and technical capabilities and continuously evaluate their personnel’s proficiency in critical techniques such as low copy number (LCN) analysis and species testing. Although this is the first time that the ISAG has conducted comparison tests for forensic testing, findings from these AFCTs may serve as the foundation for continuous improvements of the overall quality of animal forensic genetic testing.Fil: Kanthaswamy, Sreetharan. Arizona State University; Estados Unidos. University of California at Davis; Estados UnidosFil: Brendel, Torsten. Eurofins Genomics Europe Applied Genomics GmbH; AlemaniaFil: Cancela, Luis. Identitas; UruguayFil: Andrade de Oliveira, Denise A.. Universidade Federal de Minas Gerais; BrasilFil: Brenig, Bertram. Universität Göttingen; AlemaniaFil: Cons, Carmen. Universidad de Zaragoza. Instituto Agroalimentario de Aragon; EspañaFil: Crespi, Julian Alejandro. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico CONICET- La Plata. Instituto de Genética Veterinaria "Ing. Fernando Noel Dulout". Universidad Nacional de La Plata. Facultad de Ciencias Veterinarias. Instituto de Genética Veterinaria; ArgentinaFil: Dajbychová, Markéta. Genomia s.r.o.; República ChecaFil: Feldl, Andreas. Eurofins Genomics Europe Applied Genomics GmbH; AlemaniaFil: Itoh, Tomohito. Livestock Improvement Association of Japan; JapónFil: Landi, Vincenzo. Università degli Studi di Bari; ItaliaFil: Martinez, Amparo. Universidad de Córdoba; EspañaFil: Natonek-Wisniewska, Malgorzata. National Research Institute of Animal Production; PoloniaFil: Oldt, Robert F.. Arizona State University; Estados Unidos. University of California at Davis; Estados UnidosFil: Radko, Anna. Vetgenomics S.L., Barcelona; EspañaFil: Ramírez, Oscar. Vetgenomics S.L., Barcelona; EspañaFil: Rodellar, Clementina. Universidad de Zaragoza. Instituto Agroalimentario de Aragon; EspañaFil: Ruiz Girón, Manuel. Hispalis Biolab S.L.U., Sevilla; EspañaFil: Schikorski, David. Laboratoire LABOFARM-GENINDEXE; FranciaFil: Turba, María Elena. Genefast SRL ; ItaliaFil: Giovambattista, Guillermo. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico CONICET- La Plata. Instituto de Genética Veterinaria "Ing. Fernando Noel Dulout". Universidad Nacional de La Plata. Facultad de Ciencias Veterinarias. Instituto de Genética Veterinaria; ArgentinaTaylor & Francis Ltd2021-07info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionhttp://purl.org/coar/resource_type/c_6501info:ar-repo/semantics/articuloapplication/pdfapplication/pdfhttp://hdl.handle.net/11336/158180Kanthaswamy, Sreetharan; Brendel, Torsten; Cancela, Luis; Andrade de Oliveira, Denise A.; Brenig, Bertram; et al.; An inter-laboratory study of DNA-based identity, parentage and species testing in animal forensic genetics; Taylor & Francis Ltd; Forensic Sciences Research; 2021; 7-2021; 1-62471-1411CONICET DigitalCONICETenginfo:eu-repo/semantics/altIdentifier/doi/10.1080/20961790.2021.1886679info:eu-repo/semantics/altIdentifier/url/https://www.tandfonline.com/doi/full/10.1080/20961790.2021.1886679info:eu-repo/semantics/openAccesshttps://creativecommons.org/licenses/by-nc-sa/2.5/ar/reponame:CONICET Digital (CONICET)instname:Consejo Nacional de Investigaciones Científicas y Técnicas2025-09-29T09:51:47Zoai:ri.conicet.gov.ar:11336/158180instacron:CONICETInstitucionalhttp://ri.conicet.gov.ar/Organismo científico-tecnológicoNo correspondehttp://ri.conicet.gov.ar/oai/requestdasensio@conicet.gov.ar; lcarlino@conicet.gov.arArgentinaNo correspondeNo correspondeNo correspondeopendoar:34982025-09-29 09:51:47.99CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicasfalse |
| dc.title.none.fl_str_mv |
An inter-laboratory study of DNA-based identity, parentage and species testing in animal forensic genetics |
| title |
An inter-laboratory study of DNA-based identity, parentage and species testing in animal forensic genetics |
| spellingShingle |
An inter-laboratory study of DNA-based identity, parentage and species testing in animal forensic genetics Kanthaswamy, Sreetharan COMPARISON TEST FORENSIC DNA ANALYSIS FORENSIC GENETICS FORENSIC SCIENCES INDIVIDUAL IDENTIFICATION PARENTAGE TEST SPECIES TEST |
| title_short |
An inter-laboratory study of DNA-based identity, parentage and species testing in animal forensic genetics |
| title_full |
An inter-laboratory study of DNA-based identity, parentage and species testing in animal forensic genetics |
| title_fullStr |
An inter-laboratory study of DNA-based identity, parentage and species testing in animal forensic genetics |
| title_full_unstemmed |
An inter-laboratory study of DNA-based identity, parentage and species testing in animal forensic genetics |
| title_sort |
An inter-laboratory study of DNA-based identity, parentage and species testing in animal forensic genetics |
| dc.creator.none.fl_str_mv |
Kanthaswamy, Sreetharan Brendel, Torsten Cancela, Luis Andrade de Oliveira, Denise A. Brenig, Bertram Cons, Carmen Crespi, Julian Alejandro Dajbychová, Markéta Feldl, Andreas Itoh, Tomohito Landi, Vincenzo Martinez, Amparo Natonek-Wisniewska, Malgorzata Oldt, Robert F. Radko, Anna Ramírez, Oscar Rodellar, Clementina Ruiz Girón, Manuel Schikorski, David Turba, María Elena Giovambattista, Guillermo |
| author |
Kanthaswamy, Sreetharan |
| author_facet |
Kanthaswamy, Sreetharan Brendel, Torsten Cancela, Luis Andrade de Oliveira, Denise A. Brenig, Bertram Cons, Carmen Crespi, Julian Alejandro Dajbychová, Markéta Feldl, Andreas Itoh, Tomohito Landi, Vincenzo Martinez, Amparo Natonek-Wisniewska, Malgorzata Oldt, Robert F. Radko, Anna Ramírez, Oscar Rodellar, Clementina Ruiz Girón, Manuel Schikorski, David Turba, María Elena Giovambattista, Guillermo |
| author_role |
author |
| author2 |
Brendel, Torsten Cancela, Luis Andrade de Oliveira, Denise A. Brenig, Bertram Cons, Carmen Crespi, Julian Alejandro Dajbychová, Markéta Feldl, Andreas Itoh, Tomohito Landi, Vincenzo Martinez, Amparo Natonek-Wisniewska, Malgorzata Oldt, Robert F. Radko, Anna Ramírez, Oscar Rodellar, Clementina Ruiz Girón, Manuel Schikorski, David Turba, María Elena Giovambattista, Guillermo |
| author2_role |
author author author author author author author author author author author author author author author author author author author author |
| dc.subject.none.fl_str_mv |
COMPARISON TEST FORENSIC DNA ANALYSIS FORENSIC GENETICS FORENSIC SCIENCES INDIVIDUAL IDENTIFICATION PARENTAGE TEST SPECIES TEST |
| topic |
COMPARISON TEST FORENSIC DNA ANALYSIS FORENSIC GENETICS FORENSIC SCIENCES INDIVIDUAL IDENTIFICATION PARENTAGE TEST SPECIES TEST |
| purl_subject.fl_str_mv |
https://purl.org/becyt/ford/4.3 https://purl.org/becyt/ford/4 |
| dc.description.none.fl_txt_mv |
The probative value of animal forensic genetic evidence relies on laboratory accuracy and reliability. Inter-laboratory comparisons allow laboratories to evaluate their performance on specific tests and analyses and to continue to monitor their output. The International Society for Animal Genetics (ISAG) administered animal forensic comparison tests (AFCTs) in 2016 and 2018 to assess the limitations and capabilities of laboratories offering forensic identification, parentage and species determination services. The AFCTs revealed that analyses of low DNA template concentrations (≤300 pg/µL) constitute a significant challenge that has prevented many laboratories from reporting correct identification and parentage results. Moreover, a lack of familiarity with species testing protocols, interpretation guidelines and representative databases prevented over a quarter of the participating laboratories from submitting correct species determination results. Several laboratories showed improvement in their genotyping accuracy over time. However, the use of forensically validated standards, such as a standard forensic short tandem repeat (STR) kit, preferably with an allelic ladder, and stricter guidelines for STR typing, may have prevented some common issues from occurring, such as genotyping inaccuracies, missing data, elevated stutter products and loading errors. The AFCTs underscore the importance of conducting routine forensic comparison tests to allow laboratories to compare results from each other. Laboratories should keep improving their scientific and technical capabilities and continuously evaluate their personnel’s proficiency in critical techniques such as low copy number (LCN) analysis and species testing. Although this is the first time that the ISAG has conducted comparison tests for forensic testing, findings from these AFCTs may serve as the foundation for continuous improvements of the overall quality of animal forensic genetic testing. Fil: Kanthaswamy, Sreetharan. Arizona State University; Estados Unidos. University of California at Davis; Estados Unidos Fil: Brendel, Torsten. Eurofins Genomics Europe Applied Genomics GmbH; Alemania Fil: Cancela, Luis. Identitas; Uruguay Fil: Andrade de Oliveira, Denise A.. Universidade Federal de Minas Gerais; Brasil Fil: Brenig, Bertram. Universität Göttingen; Alemania Fil: Cons, Carmen. Universidad de Zaragoza. Instituto Agroalimentario de Aragon; España Fil: Crespi, Julian Alejandro. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico CONICET- La Plata. Instituto de Genética Veterinaria "Ing. Fernando Noel Dulout". Universidad Nacional de La Plata. Facultad de Ciencias Veterinarias. Instituto de Genética Veterinaria; Argentina Fil: Dajbychová, Markéta. Genomia s.r.o.; República Checa Fil: Feldl, Andreas. Eurofins Genomics Europe Applied Genomics GmbH; Alemania Fil: Itoh, Tomohito. Livestock Improvement Association of Japan; Japón Fil: Landi, Vincenzo. Università degli Studi di Bari; Italia Fil: Martinez, Amparo. Universidad de Córdoba; España Fil: Natonek-Wisniewska, Malgorzata. National Research Institute of Animal Production; Polonia Fil: Oldt, Robert F.. Arizona State University; Estados Unidos. University of California at Davis; Estados Unidos Fil: Radko, Anna. Vetgenomics S.L., Barcelona; España Fil: Ramírez, Oscar. Vetgenomics S.L., Barcelona; España Fil: Rodellar, Clementina. Universidad de Zaragoza. Instituto Agroalimentario de Aragon; España Fil: Ruiz Girón, Manuel. Hispalis Biolab S.L.U., Sevilla; España Fil: Schikorski, David. Laboratoire LABOFARM-GENINDEXE; Francia Fil: Turba, María Elena. Genefast SRL ; Italia Fil: Giovambattista, Guillermo. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico CONICET- La Plata. Instituto de Genética Veterinaria "Ing. Fernando Noel Dulout". Universidad Nacional de La Plata. Facultad de Ciencias Veterinarias. Instituto de Genética Veterinaria; Argentina |
| description |
The probative value of animal forensic genetic evidence relies on laboratory accuracy and reliability. Inter-laboratory comparisons allow laboratories to evaluate their performance on specific tests and analyses and to continue to monitor their output. The International Society for Animal Genetics (ISAG) administered animal forensic comparison tests (AFCTs) in 2016 and 2018 to assess the limitations and capabilities of laboratories offering forensic identification, parentage and species determination services. The AFCTs revealed that analyses of low DNA template concentrations (≤300 pg/µL) constitute a significant challenge that has prevented many laboratories from reporting correct identification and parentage results. Moreover, a lack of familiarity with species testing protocols, interpretation guidelines and representative databases prevented over a quarter of the participating laboratories from submitting correct species determination results. Several laboratories showed improvement in their genotyping accuracy over time. However, the use of forensically validated standards, such as a standard forensic short tandem repeat (STR) kit, preferably with an allelic ladder, and stricter guidelines for STR typing, may have prevented some common issues from occurring, such as genotyping inaccuracies, missing data, elevated stutter products and loading errors. The AFCTs underscore the importance of conducting routine forensic comparison tests to allow laboratories to compare results from each other. Laboratories should keep improving their scientific and technical capabilities and continuously evaluate their personnel’s proficiency in critical techniques such as low copy number (LCN) analysis and species testing. Although this is the first time that the ISAG has conducted comparison tests for forensic testing, findings from these AFCTs may serve as the foundation for continuous improvements of the overall quality of animal forensic genetic testing. |
| publishDate |
2021 |
| dc.date.none.fl_str_mv |
2021-07 |
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info:eu-repo/semantics/article info:eu-repo/semantics/publishedVersion http://purl.org/coar/resource_type/c_6501 info:ar-repo/semantics/articulo |
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article |
| status_str |
publishedVersion |
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http://hdl.handle.net/11336/158180 Kanthaswamy, Sreetharan; Brendel, Torsten; Cancela, Luis; Andrade de Oliveira, Denise A.; Brenig, Bertram; et al.; An inter-laboratory study of DNA-based identity, parentage and species testing in animal forensic genetics; Taylor & Francis Ltd; Forensic Sciences Research; 2021; 7-2021; 1-6 2471-1411 CONICET Digital CONICET |
| url |
http://hdl.handle.net/11336/158180 |
| identifier_str_mv |
Kanthaswamy, Sreetharan; Brendel, Torsten; Cancela, Luis; Andrade de Oliveira, Denise A.; Brenig, Bertram; et al.; An inter-laboratory study of DNA-based identity, parentage and species testing in animal forensic genetics; Taylor & Francis Ltd; Forensic Sciences Research; 2021; 7-2021; 1-6 2471-1411 CONICET Digital CONICET |
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eng |
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eng |
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info:eu-repo/semantics/altIdentifier/doi/10.1080/20961790.2021.1886679 info:eu-repo/semantics/altIdentifier/url/https://www.tandfonline.com/doi/full/10.1080/20961790.2021.1886679 |
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Taylor & Francis Ltd |
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Taylor & Francis Ltd |
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