Distinct gut microbial signature and altered short chain fatty acid metabolism at disease onset in a rat preclinical model of superimposed preeclampsia

Autores
Alhasan, Moumen M.; Landa, Maria Silvina; Garcia, Silvia Ines; Gerlach, Roman G.; Harb, Hani; Fahlbusch, Fabian B.; Conrad, Melanie L.; Barrientos, Gabriela Laura
Año de publicación
2024
Idioma
inglés
Tipo de recurso
artículo
Estado
versión publicada
Descripción
Chronic hypertension is an increasingly prevalent condition that constitutes a risk factor for superimposed preeclampsia during pregnancy. In this study, we assessed the gut microbiome in a rat model of superimposed preeclampsia to characterize the microbial signature associated with defective placentation processes identified at the preclinical disease stage. The blood pressure profile, renal function parameters and fetal phenotype were evaluated in pregnant Stroke-prone Spontaneously Hypertensive Rats (SHRSP) and their normotensive controls. On gestation day (GD)14, feces were collected and gut microbiome composition and short-chain fatty acid concentrations were determined by 16S rRNA sequencing and gas chromatography respectively. At disease onset on GD14, the fecal gut microbiome of SHRSP showed a lower alpha diversity and significant differences in beta diversity when compared with control animals. In the feces, Prevotella, Bifidobacterium, Parasutterella and Roseburia were enriched in SHRSP pregnancies compared to controls, showing a strong correlation with clinical parameters. Bacteria from the families Ruminococcaceae, Oscillospiraceae and the genera Blautia and Faecalibacterium were depleted. Considering short-chain fatty acids, acetate, propionate and valerate were increased in the SHRSP model, showing a strong positive correlation with the relative abundance of enriched taxa. We show that on GD14, at the asymptomatic SPE onset stage, pregnant SHRSP display a distinct gut microbiome signature and altered short chain fatty acid metabolism compared to control animals.
Fil: Alhasan, Moumen M.. Humboldt-Universität zu Berlin; Alemania
Fil: Landa, Maria Silvina. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Houssay. Instituto de Investigaciones Médicas. Universidad de Buenos Aires. Facultad de Medicina. Instituto de Investigaciones Médicas; Argentina
Fil: Garcia, Silvia Ines. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Houssay. Instituto de Investigaciones Médicas. Universidad de Buenos Aires. Facultad de Medicina. Instituto de Investigaciones Médicas; Argentina. Hospital Alemán; Argentina
Fil: Gerlach, Roman G.. Friedrich-Alexander-University; Alemania. Universitat Erlangen Nuremberg; Alemania
Fil: Harb, Hani. Inst Of Microbiology, Infectious Diseases; Alemania
Fil: Fahlbusch, Fabian B.. Technische Universität Dresden; Alemania
Fil: Conrad, Melanie L.. Universitat Augsburg; . Humboldt-Universität zu Berlin; Alemania
Fil: Barrientos, Gabriela Laura. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Houssay. Instituto de Investigaciones Médicas. Universidad de Buenos Aires. Facultad de Medicina. Instituto de Investigaciones Médicas; Argentina. Hospital Alemán; Argentina
Materia
Preeclampsia
Microbiota
SHR
Rata
Nivel de accesibilidad
acceso abierto
Condiciones de uso
https://creativecommons.org/licenses/by-nc-nd/2.5/ar/
Repositorio
CONICET Digital (CONICET)
Institución
Consejo Nacional de Investigaciones Científicas y Técnicas
OAI Identificador
oai:ri.conicet.gov.ar:11336/262163
Acceder
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network_acronym_str CONICETDig
repository_id_str 3498
network_name_str CONICET Digital (CONICET)
spelling Distinct gut microbial signature and altered short chain fatty acid metabolism at disease onset in a rat preclinical model of superimposed preeclampsiaAlhasan, Moumen M.Landa, Maria SilvinaGarcia, Silvia InesGerlach, Roman G.Harb, HaniFahlbusch, Fabian B.Conrad, Melanie L.Barrientos, Gabriela LauraPreeclampsiaMicrobiotaSHRRatahttps://purl.org/becyt/ford/3.1https://purl.org/becyt/ford/3Chronic hypertension is an increasingly prevalent condition that constitutes a risk factor for superimposed preeclampsia during pregnancy. In this study, we assessed the gut microbiome in a rat model of superimposed preeclampsia to characterize the microbial signature associated with defective placentation processes identified at the preclinical disease stage. The blood pressure profile, renal function parameters and fetal phenotype were evaluated in pregnant Stroke-prone Spontaneously Hypertensive Rats (SHRSP) and their normotensive controls. On gestation day (GD)14, feces were collected and gut microbiome composition and short-chain fatty acid concentrations were determined by 16S rRNA sequencing and gas chromatography respectively. At disease onset on GD14, the fecal gut microbiome of SHRSP showed a lower alpha diversity and significant differences in beta diversity when compared with control animals. In the feces, Prevotella, Bifidobacterium, Parasutterella and Roseburia were enriched in SHRSP pregnancies compared to controls, showing a strong correlation with clinical parameters. Bacteria from the families Ruminococcaceae, Oscillospiraceae and the genera Blautia and Faecalibacterium were depleted. Considering short-chain fatty acids, acetate, propionate and valerate were increased in the SHRSP model, showing a strong positive correlation with the relative abundance of enriched taxa. We show that on GD14, at the asymptomatic SPE onset stage, pregnant SHRSP display a distinct gut microbiome signature and altered short chain fatty acid metabolism compared to control animals.Fil: Alhasan, Moumen M.. Humboldt-Universität zu Berlin; AlemaniaFil: Landa, Maria Silvina. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Houssay. Instituto de Investigaciones Médicas. Universidad de Buenos Aires. Facultad de Medicina. Instituto de Investigaciones Médicas; ArgentinaFil: Garcia, Silvia Ines. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Houssay. Instituto de Investigaciones Médicas. Universidad de Buenos Aires. Facultad de Medicina. Instituto de Investigaciones Médicas; Argentina. Hospital Alemán; ArgentinaFil: Gerlach, Roman G.. Friedrich-Alexander-University; Alemania. Universitat Erlangen Nuremberg; AlemaniaFil: Harb, Hani. Inst Of Microbiology, Infectious Diseases; AlemaniaFil: Fahlbusch, Fabian B.. Technische Universität Dresden; AlemaniaFil: Conrad, Melanie L.. Universitat Augsburg; . Humboldt-Universität zu Berlin; AlemaniaFil: Barrientos, Gabriela Laura. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Houssay. Instituto de Investigaciones Médicas. Universidad de Buenos Aires. Facultad de Medicina. Instituto de Investigaciones Médicas; Argentina. Hospital Alemán; ArgentinaNature Publishing Group2024-12info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionhttp://purl.org/coar/resource_type/c_6501info:ar-repo/semantics/articuloapplication/pdfapplication/pdfhttp://hdl.handle.net/11336/262163Alhasan, Moumen M.; Landa, Maria Silvina; Garcia, Silvia Ines; Gerlach, Roman G.; Harb, Hani; et al.; Distinct gut microbial signature and altered short chain fatty acid metabolism at disease onset in a rat preclinical model of superimposed preeclampsia; Nature Publishing Group; Scientific Reports; 14; 1; 12-2024; 1-122045-2322CONICET DigitalCONICETenginfo:eu-repo/semantics/altIdentifier/url/https://www.nature.com/articles/s41598-024-83981-5info:eu-repo/semantics/altIdentifier/doi/10.1038/s41598-024-83981-5info:eu-repo/semantics/openAccesshttps://creativecommons.org/licenses/by-nc-nd/2.5/ar/reponame:CONICET Digital (CONICET)instname:Consejo Nacional de Investigaciones Científicas y Técnicas2025-09-03T09:58:40Zoai:ri.conicet.gov.ar:11336/262163instacron:CONICETInstitucionalhttp://ri.conicet.gov.ar/Organismo científico-tecnológicoNo correspondehttp://ri.conicet.gov.ar/oai/requestdasensio@conicet.gov.ar; lcarlino@conicet.gov.arArgentinaNo correspondeNo correspondeNo correspondeopendoar:34982025-09-03 09:58:40.647CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicasfalse
dc.title.none.fl_str_mv Distinct gut microbial signature and altered short chain fatty acid metabolism at disease onset in a rat preclinical model of superimposed preeclampsia
title Distinct gut microbial signature and altered short chain fatty acid metabolism at disease onset in a rat preclinical model of superimposed preeclampsia
spellingShingle Distinct gut microbial signature and altered short chain fatty acid metabolism at disease onset in a rat preclinical model of superimposed preeclampsia
Alhasan, Moumen M.
Preeclampsia
Microbiota
SHR
Rata
title_short Distinct gut microbial signature and altered short chain fatty acid metabolism at disease onset in a rat preclinical model of superimposed preeclampsia
title_full Distinct gut microbial signature and altered short chain fatty acid metabolism at disease onset in a rat preclinical model of superimposed preeclampsia
title_fullStr Distinct gut microbial signature and altered short chain fatty acid metabolism at disease onset in a rat preclinical model of superimposed preeclampsia
title_full_unstemmed Distinct gut microbial signature and altered short chain fatty acid metabolism at disease onset in a rat preclinical model of superimposed preeclampsia
title_sort Distinct gut microbial signature and altered short chain fatty acid metabolism at disease onset in a rat preclinical model of superimposed preeclampsia
dc.creator.none.fl_str_mv Alhasan, Moumen M.
Landa, Maria Silvina
Garcia, Silvia Ines
Gerlach, Roman G.
Harb, Hani
Fahlbusch, Fabian B.
Conrad, Melanie L.
Barrientos, Gabriela Laura
author Alhasan, Moumen M.
author_facet Alhasan, Moumen M.
Landa, Maria Silvina
Garcia, Silvia Ines
Gerlach, Roman G.
Harb, Hani
Fahlbusch, Fabian B.
Conrad, Melanie L.
Barrientos, Gabriela Laura
author_role author
author2 Landa, Maria Silvina
Garcia, Silvia Ines
Gerlach, Roman G.
Harb, Hani
Fahlbusch, Fabian B.
Conrad, Melanie L.
Barrientos, Gabriela Laura
author2_role author
author
author
author
author
author
author
dc.subject.none.fl_str_mv Preeclampsia
Microbiota
SHR
Rata
topic Preeclampsia
Microbiota
SHR
Rata
purl_subject.fl_str_mv https://purl.org/becyt/ford/3.1
https://purl.org/becyt/ford/3
dc.description.none.fl_txt_mv Chronic hypertension is an increasingly prevalent condition that constitutes a risk factor for superimposed preeclampsia during pregnancy. In this study, we assessed the gut microbiome in a rat model of superimposed preeclampsia to characterize the microbial signature associated with defective placentation processes identified at the preclinical disease stage. The blood pressure profile, renal function parameters and fetal phenotype were evaluated in pregnant Stroke-prone Spontaneously Hypertensive Rats (SHRSP) and their normotensive controls. On gestation day (GD)14, feces were collected and gut microbiome composition and short-chain fatty acid concentrations were determined by 16S rRNA sequencing and gas chromatography respectively. At disease onset on GD14, the fecal gut microbiome of SHRSP showed a lower alpha diversity and significant differences in beta diversity when compared with control animals. In the feces, Prevotella, Bifidobacterium, Parasutterella and Roseburia were enriched in SHRSP pregnancies compared to controls, showing a strong correlation with clinical parameters. Bacteria from the families Ruminococcaceae, Oscillospiraceae and the genera Blautia and Faecalibacterium were depleted. Considering short-chain fatty acids, acetate, propionate and valerate were increased in the SHRSP model, showing a strong positive correlation with the relative abundance of enriched taxa. We show that on GD14, at the asymptomatic SPE onset stage, pregnant SHRSP display a distinct gut microbiome signature and altered short chain fatty acid metabolism compared to control animals.
Fil: Alhasan, Moumen M.. Humboldt-Universität zu Berlin; Alemania
Fil: Landa, Maria Silvina. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Houssay. Instituto de Investigaciones Médicas. Universidad de Buenos Aires. Facultad de Medicina. Instituto de Investigaciones Médicas; Argentina
Fil: Garcia, Silvia Ines. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Houssay. Instituto de Investigaciones Médicas. Universidad de Buenos Aires. Facultad de Medicina. Instituto de Investigaciones Médicas; Argentina. Hospital Alemán; Argentina
Fil: Gerlach, Roman G.. Friedrich-Alexander-University; Alemania. Universitat Erlangen Nuremberg; Alemania
Fil: Harb, Hani. Inst Of Microbiology, Infectious Diseases; Alemania
Fil: Fahlbusch, Fabian B.. Technische Universität Dresden; Alemania
Fil: Conrad, Melanie L.. Universitat Augsburg; . Humboldt-Universität zu Berlin; Alemania
Fil: Barrientos, Gabriela Laura. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Houssay. Instituto de Investigaciones Médicas. Universidad de Buenos Aires. Facultad de Medicina. Instituto de Investigaciones Médicas; Argentina. Hospital Alemán; Argentina
description Chronic hypertension is an increasingly prevalent condition that constitutes a risk factor for superimposed preeclampsia during pregnancy. In this study, we assessed the gut microbiome in a rat model of superimposed preeclampsia to characterize the microbial signature associated with defective placentation processes identified at the preclinical disease stage. The blood pressure profile, renal function parameters and fetal phenotype were evaluated in pregnant Stroke-prone Spontaneously Hypertensive Rats (SHRSP) and their normotensive controls. On gestation day (GD)14, feces were collected and gut microbiome composition and short-chain fatty acid concentrations were determined by 16S rRNA sequencing and gas chromatography respectively. At disease onset on GD14, the fecal gut microbiome of SHRSP showed a lower alpha diversity and significant differences in beta diversity when compared with control animals. In the feces, Prevotella, Bifidobacterium, Parasutterella and Roseburia were enriched in SHRSP pregnancies compared to controls, showing a strong correlation with clinical parameters. Bacteria from the families Ruminococcaceae, Oscillospiraceae and the genera Blautia and Faecalibacterium were depleted. Considering short-chain fatty acids, acetate, propionate and valerate were increased in the SHRSP model, showing a strong positive correlation with the relative abundance of enriched taxa. We show that on GD14, at the asymptomatic SPE onset stage, pregnant SHRSP display a distinct gut microbiome signature and altered short chain fatty acid metabolism compared to control animals.
publishDate 2024
dc.date.none.fl_str_mv 2024-12
dc.type.none.fl_str_mv info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
http://purl.org/coar/resource_type/c_6501
info:ar-repo/semantics/articulo
format article
status_str publishedVersion
dc.identifier.none.fl_str_mv http://hdl.handle.net/11336/262163
Alhasan, Moumen M.; Landa, Maria Silvina; Garcia, Silvia Ines; Gerlach, Roman G.; Harb, Hani; et al.; Distinct gut microbial signature and altered short chain fatty acid metabolism at disease onset in a rat preclinical model of superimposed preeclampsia; Nature Publishing Group; Scientific Reports; 14; 1; 12-2024; 1-12
2045-2322
CONICET Digital
CONICET
url http://hdl.handle.net/11336/262163
identifier_str_mv Alhasan, Moumen M.; Landa, Maria Silvina; Garcia, Silvia Ines; Gerlach, Roman G.; Harb, Hani; et al.; Distinct gut microbial signature and altered short chain fatty acid metabolism at disease onset in a rat preclinical model of superimposed preeclampsia; Nature Publishing Group; Scientific Reports; 14; 1; 12-2024; 1-12
2045-2322
CONICET Digital
CONICET
dc.language.none.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv info:eu-repo/semantics/altIdentifier/url/https://www.nature.com/articles/s41598-024-83981-5
info:eu-repo/semantics/altIdentifier/doi/10.1038/s41598-024-83981-5
dc.rights.none.fl_str_mv info:eu-repo/semantics/openAccess
https://creativecommons.org/licenses/by-nc-nd/2.5/ar/
eu_rights_str_mv openAccess
rights_invalid_str_mv https://creativecommons.org/licenses/by-nc-nd/2.5/ar/
dc.format.none.fl_str_mv application/pdf
application/pdf
dc.publisher.none.fl_str_mv Nature Publishing Group
publisher.none.fl_str_mv Nature Publishing Group
dc.source.none.fl_str_mv reponame:CONICET Digital (CONICET)
instname:Consejo Nacional de Investigaciones Científicas y Técnicas
reponame_str CONICET Digital (CONICET)
collection CONICET Digital (CONICET)
instname_str Consejo Nacional de Investigaciones Científicas y Técnicas
repository.name.fl_str_mv CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicas
repository.mail.fl_str_mv dasensio@conicet.gov.ar; lcarlino@conicet.gov.ar
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score 12.885934

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