Effect of ketotifen fumarate on experimental autoimmune orchitis and torsion of the spermatic cord

Autores
Moreno, Diego Javier; Sobarzo, Cristian Marcelo; Lustig, Livia; Rodriguez Peña, Marcelo Javier; Guazzone, Vanesa Anabella
Año de publicación
2020
Idioma
inglés
Tipo de recurso
artículo
Estado
versión publicada
Descripción
The aim of this work was to study effects of ketotifen fumarate (KF) on prevention of tissue damage in testes of rats with experimental autoimmune orchitis (EAO) and on the contralateral testis in a model of prolonged testicular cord torsion (TCT). Rats with EAO or TCT were injected intraperitoneally once daily with KF or saline solution (vehicle group). Incidence and severity of testicular damage were evaluated by histopathology using an EAO score or a Johnsen score. Mast cells (MC) were identified by histochemistry and quantified. In EAO model, KF significantly reduced severity of histopathological testicular damage compared to rats in the vehicle group. KF also reduced the number of testicular MC compared to vehicle group. Similarly, in TCT model, multifocal damage of the contralateral testis was observed 30 days after testicular torsion characterized by sloughing of the germinal epithelium, seminiferous tubule atrophy, and interstitial edema. Focal signs of inflammation and fibrosis of seminiferous tubular walls were also observed. In contrast, sections of contralateral testis of rats injected with KF and killed 30 days after surgery showed normal histological features. A significant decrease in the number of MC was observed in rats treated with KF compared to untreated animals. In conclusion, we demonstrated that treatment with KF reduced testicular inflammatory process and MC infiltrates in both EAO and TCT models. The results suggest a promising treatment for infertile male patients with testicular pathologies associated with inflammation and germ cell loss.
Fil: Moreno, Diego Javier. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Houssay. Instituto de Investigaciones Biomédicas. Universidad de Buenos Aires. Facultad de Medicina. Instituto de Investigaciones Biomédicas; Argentina
Fil: Sobarzo, Cristian Marcelo. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Houssay. Instituto de Investigaciones Biomédicas. Universidad de Buenos Aires. Facultad de Medicina. Instituto de Investigaciones Biomédicas; Argentina
Fil: Lustig, Livia. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Houssay. Instituto de Investigaciones Biomédicas. Universidad de Buenos Aires. Facultad de Medicina. Instituto de Investigaciones Biomédicas; Argentina
Fil: Rodriguez Peña, Marcelo Javier. Ministerio de Defensa. Ejercito Argentino. Hospital Militar Campo de Mayo Cirujano Primero Doctor Juan Madera; Argentina
Fil: Guazzone, Vanesa Anabella. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Houssay. Instituto de Investigaciones Biomédicas. Universidad de Buenos Aires. Facultad de Medicina. Instituto de Investigaciones Biomédicas; Argentina
Materia
KETOTIFEN FUMARATE
MALE INFERTILITY
MAST CELLS
ORCHITIS
TESTICULAR CORD TORSION
TESTIS
Nivel de accesibilidad
acceso abierto
Condiciones de uso
https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
Repositorio
CONICET Digital (CONICET)
Institución
Consejo Nacional de Investigaciones Científicas y Técnicas
OAI Identificador
oai:ri.conicet.gov.ar:11336/113442
Acceder
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oai_identifier_str oai:ri.conicet.gov.ar:11336/113442
network_acronym_str CONICETDig
repository_id_str 3498
network_name_str CONICET Digital (CONICET)
spelling Effect of ketotifen fumarate on experimental autoimmune orchitis and torsion of the spermatic cordMoreno, Diego JavierSobarzo, Cristian MarceloLustig, LiviaRodriguez Peña, Marcelo JavierGuazzone, Vanesa AnabellaKETOTIFEN FUMARATEMALE INFERTILITYMAST CELLSORCHITISTESTICULAR CORD TORSIONTESTIShttps://purl.org/becyt/ford/3.1https://purl.org/becyt/ford/3The aim of this work was to study effects of ketotifen fumarate (KF) on prevention of tissue damage in testes of rats with experimental autoimmune orchitis (EAO) and on the contralateral testis in a model of prolonged testicular cord torsion (TCT). Rats with EAO or TCT were injected intraperitoneally once daily with KF or saline solution (vehicle group). Incidence and severity of testicular damage were evaluated by histopathology using an EAO score or a Johnsen score. Mast cells (MC) were identified by histochemistry and quantified. In EAO model, KF significantly reduced severity of histopathological testicular damage compared to rats in the vehicle group. KF also reduced the number of testicular MC compared to vehicle group. Similarly, in TCT model, multifocal damage of the contralateral testis was observed 30 days after testicular torsion characterized by sloughing of the germinal epithelium, seminiferous tubule atrophy, and interstitial edema. Focal signs of inflammation and fibrosis of seminiferous tubular walls were also observed. In contrast, sections of contralateral testis of rats injected with KF and killed 30 days after surgery showed normal histological features. A significant decrease in the number of MC was observed in rats treated with KF compared to untreated animals. In conclusion, we demonstrated that treatment with KF reduced testicular inflammatory process and MC infiltrates in both EAO and TCT models. The results suggest a promising treatment for infertile male patients with testicular pathologies associated with inflammation and germ cell loss.Fil: Moreno, Diego Javier. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Houssay. Instituto de Investigaciones Biomédicas. Universidad de Buenos Aires. Facultad de Medicina. Instituto de Investigaciones Biomédicas; ArgentinaFil: Sobarzo, Cristian Marcelo. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Houssay. Instituto de Investigaciones Biomédicas. Universidad de Buenos Aires. Facultad de Medicina. Instituto de Investigaciones Biomédicas; ArgentinaFil: Lustig, Livia. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Houssay. Instituto de Investigaciones Biomédicas. Universidad de Buenos Aires. Facultad de Medicina. Instituto de Investigaciones Biomédicas; ArgentinaFil: Rodriguez Peña, Marcelo Javier. Ministerio de Defensa. Ejercito Argentino. Hospital Militar Campo de Mayo Cirujano Primero Doctor Juan Madera; ArgentinaFil: Guazzone, Vanesa Anabella. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Houssay. Instituto de Investigaciones Biomédicas. Universidad de Buenos Aires. Facultad de Medicina. Instituto de Investigaciones Biomédicas; ArgentinaNature Publishing Group2020-01info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionhttp://purl.org/coar/resource_type/c_6501info:ar-repo/semantics/articuloapplication/pdfapplication/pdfapplication/pdfapplication/pdfhttp://hdl.handle.net/11336/113442Moreno, Diego Javier; Sobarzo, Cristian Marcelo; Lustig, Livia; Rodriguez Peña, Marcelo Javier; Guazzone, Vanesa Anabella; Effect of ketotifen fumarate on experimental autoimmune orchitis and torsion of the spermatic cord; Nature Publishing Group; Asian Journal of Andrology; 22; 1; 1-2020; 112-1171008-682X1745-7262CONICET DigitalCONICETenginfo:eu-repo/semantics/altIdentifier/url/https://pubmed.ncbi.nlm.nih.gov/31115365/info:eu-repo/semantics/altIdentifier/doi/10.4103/aja.aja_30_19info:eu-repo/semantics/altIdentifier/url/http://www.ajandrology.com/article.asp?issn=1008-682X;year=2020;volume=22;issue=1;spage=112;epage=117;aulast=Morenoinfo:eu-repo/semantics/openAccesshttps://creativecommons.org/licenses/by-nc-sa/2.5/ar/reponame:CONICET Digital (CONICET)instname:Consejo Nacional de Investigaciones Científicas y Técnicas2025-09-03T09:47:52Zoai:ri.conicet.gov.ar:11336/113442instacron:CONICETInstitucionalhttp://ri.conicet.gov.ar/Organismo científico-tecnológicoNo correspondehttp://ri.conicet.gov.ar/oai/requestdasensio@conicet.gov.ar; lcarlino@conicet.gov.arArgentinaNo correspondeNo correspondeNo correspondeopendoar:34982025-09-03 09:47:52.246CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicasfalse
dc.title.none.fl_str_mv Effect of ketotifen fumarate on experimental autoimmune orchitis and torsion of the spermatic cord
title Effect of ketotifen fumarate on experimental autoimmune orchitis and torsion of the spermatic cord
spellingShingle Effect of ketotifen fumarate on experimental autoimmune orchitis and torsion of the spermatic cord
Moreno, Diego Javier
KETOTIFEN FUMARATE
MALE INFERTILITY
MAST CELLS
ORCHITIS
TESTICULAR CORD TORSION
TESTIS
title_short Effect of ketotifen fumarate on experimental autoimmune orchitis and torsion of the spermatic cord
title_full Effect of ketotifen fumarate on experimental autoimmune orchitis and torsion of the spermatic cord
title_fullStr Effect of ketotifen fumarate on experimental autoimmune orchitis and torsion of the spermatic cord
title_full_unstemmed Effect of ketotifen fumarate on experimental autoimmune orchitis and torsion of the spermatic cord
title_sort Effect of ketotifen fumarate on experimental autoimmune orchitis and torsion of the spermatic cord
dc.creator.none.fl_str_mv Moreno, Diego Javier
Sobarzo, Cristian Marcelo
Lustig, Livia
Rodriguez Peña, Marcelo Javier
Guazzone, Vanesa Anabella
author Moreno, Diego Javier
author_facet Moreno, Diego Javier
Sobarzo, Cristian Marcelo
Lustig, Livia
Rodriguez Peña, Marcelo Javier
Guazzone, Vanesa Anabella
author_role author
author2 Sobarzo, Cristian Marcelo
Lustig, Livia
Rodriguez Peña, Marcelo Javier
Guazzone, Vanesa Anabella
author2_role author
author
author
author
dc.subject.none.fl_str_mv KETOTIFEN FUMARATE
MALE INFERTILITY
MAST CELLS
ORCHITIS
TESTICULAR CORD TORSION
TESTIS
topic KETOTIFEN FUMARATE
MALE INFERTILITY
MAST CELLS
ORCHITIS
TESTICULAR CORD TORSION
TESTIS
purl_subject.fl_str_mv https://purl.org/becyt/ford/3.1
https://purl.org/becyt/ford/3
dc.description.none.fl_txt_mv The aim of this work was to study effects of ketotifen fumarate (KF) on prevention of tissue damage in testes of rats with experimental autoimmune orchitis (EAO) and on the contralateral testis in a model of prolonged testicular cord torsion (TCT). Rats with EAO or TCT were injected intraperitoneally once daily with KF or saline solution (vehicle group). Incidence and severity of testicular damage were evaluated by histopathology using an EAO score or a Johnsen score. Mast cells (MC) were identified by histochemistry and quantified. In EAO model, KF significantly reduced severity of histopathological testicular damage compared to rats in the vehicle group. KF also reduced the number of testicular MC compared to vehicle group. Similarly, in TCT model, multifocal damage of the contralateral testis was observed 30 days after testicular torsion characterized by sloughing of the germinal epithelium, seminiferous tubule atrophy, and interstitial edema. Focal signs of inflammation and fibrosis of seminiferous tubular walls were also observed. In contrast, sections of contralateral testis of rats injected with KF and killed 30 days after surgery showed normal histological features. A significant decrease in the number of MC was observed in rats treated with KF compared to untreated animals. In conclusion, we demonstrated that treatment with KF reduced testicular inflammatory process and MC infiltrates in both EAO and TCT models. The results suggest a promising treatment for infertile male patients with testicular pathologies associated with inflammation and germ cell loss.
Fil: Moreno, Diego Javier. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Houssay. Instituto de Investigaciones Biomédicas. Universidad de Buenos Aires. Facultad de Medicina. Instituto de Investigaciones Biomédicas; Argentina
Fil: Sobarzo, Cristian Marcelo. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Houssay. Instituto de Investigaciones Biomédicas. Universidad de Buenos Aires. Facultad de Medicina. Instituto de Investigaciones Biomédicas; Argentina
Fil: Lustig, Livia. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Houssay. Instituto de Investigaciones Biomédicas. Universidad de Buenos Aires. Facultad de Medicina. Instituto de Investigaciones Biomédicas; Argentina
Fil: Rodriguez Peña, Marcelo Javier. Ministerio de Defensa. Ejercito Argentino. Hospital Militar Campo de Mayo Cirujano Primero Doctor Juan Madera; Argentina
Fil: Guazzone, Vanesa Anabella. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Houssay. Instituto de Investigaciones Biomédicas. Universidad de Buenos Aires. Facultad de Medicina. Instituto de Investigaciones Biomédicas; Argentina
description The aim of this work was to study effects of ketotifen fumarate (KF) on prevention of tissue damage in testes of rats with experimental autoimmune orchitis (EAO) and on the contralateral testis in a model of prolonged testicular cord torsion (TCT). Rats with EAO or TCT were injected intraperitoneally once daily with KF or saline solution (vehicle group). Incidence and severity of testicular damage were evaluated by histopathology using an EAO score or a Johnsen score. Mast cells (MC) were identified by histochemistry and quantified. In EAO model, KF significantly reduced severity of histopathological testicular damage compared to rats in the vehicle group. KF also reduced the number of testicular MC compared to vehicle group. Similarly, in TCT model, multifocal damage of the contralateral testis was observed 30 days after testicular torsion characterized by sloughing of the germinal epithelium, seminiferous tubule atrophy, and interstitial edema. Focal signs of inflammation and fibrosis of seminiferous tubular walls were also observed. In contrast, sections of contralateral testis of rats injected with KF and killed 30 days after surgery showed normal histological features. A significant decrease in the number of MC was observed in rats treated with KF compared to untreated animals. In conclusion, we demonstrated that treatment with KF reduced testicular inflammatory process and MC infiltrates in both EAO and TCT models. The results suggest a promising treatment for infertile male patients with testicular pathologies associated with inflammation and germ cell loss.
publishDate 2020
dc.date.none.fl_str_mv 2020-01
dc.type.none.fl_str_mv info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
http://purl.org/coar/resource_type/c_6501
info:ar-repo/semantics/articulo
format article
status_str publishedVersion
dc.identifier.none.fl_str_mv http://hdl.handle.net/11336/113442
Moreno, Diego Javier; Sobarzo, Cristian Marcelo; Lustig, Livia; Rodriguez Peña, Marcelo Javier; Guazzone, Vanesa Anabella; Effect of ketotifen fumarate on experimental autoimmune orchitis and torsion of the spermatic cord; Nature Publishing Group; Asian Journal of Andrology; 22; 1; 1-2020; 112-117
1008-682X
1745-7262
CONICET Digital
CONICET
url http://hdl.handle.net/11336/113442
identifier_str_mv Moreno, Diego Javier; Sobarzo, Cristian Marcelo; Lustig, Livia; Rodriguez Peña, Marcelo Javier; Guazzone, Vanesa Anabella; Effect of ketotifen fumarate on experimental autoimmune orchitis and torsion of the spermatic cord; Nature Publishing Group; Asian Journal of Andrology; 22; 1; 1-2020; 112-117
1008-682X
1745-7262
CONICET Digital
CONICET
dc.language.none.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv info:eu-repo/semantics/altIdentifier/url/https://pubmed.ncbi.nlm.nih.gov/31115365/
info:eu-repo/semantics/altIdentifier/doi/10.4103/aja.aja_30_19
info:eu-repo/semantics/altIdentifier/url/http://www.ajandrology.com/article.asp?issn=1008-682X;year=2020;volume=22;issue=1;spage=112;epage=117;aulast=Moreno
dc.rights.none.fl_str_mv info:eu-repo/semantics/openAccess
https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
eu_rights_str_mv openAccess
rights_invalid_str_mv https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
dc.format.none.fl_str_mv application/pdf
application/pdf
application/pdf
application/pdf
dc.publisher.none.fl_str_mv Nature Publishing Group
publisher.none.fl_str_mv Nature Publishing Group
dc.source.none.fl_str_mv reponame:CONICET Digital (CONICET)
instname:Consejo Nacional de Investigaciones Científicas y Técnicas
reponame_str CONICET Digital (CONICET)
collection CONICET Digital (CONICET)
instname_str Consejo Nacional de Investigaciones Científicas y Técnicas
repository.name.fl_str_mv CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicas
repository.mail.fl_str_mv dasensio@conicet.gov.ar; lcarlino@conicet.gov.ar
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