Histopathology of the resistance to Colletotrichum gloeosporioides of wild strawberries and species related to commercial strawberry

Autores
Pardo, Esteban Mariano; Grellet Bournonville, Carlos Froilan; Salazar, Sergio Miguel; Castagnaro, Atilio Pedro; Diaz Ricci, Juan Carlos; Arias, Marta Eugenia
Año de publicación
2012
Idioma
inglés
Tipo de recurso
artículo
Estado
versión publicada
Descripción
Three species of wild strawberries, as well as three related species, were tested against virulent isolate of Colletotrichum gloeosporioides. The purpose of this study was to assess genetic resources exhibiting resistance to anthracnose. The results of inoculation experiments showed that the species Fragaria vesca, Fragaria virginiana and Fragaria chiloensis were susceptible to fungi; whereas species Duchesnea indica, Duchesnea chrysantha and Potentilla tucumanensis showed higher levels of resistance. A histopathological study revealed a different response between susceptible and tolerant species. A significant thickening of the cell wall in epidermal cells, changes of stomata and mesophyll cells, the presence of idioblastic cells with unknown content and the accumulation of large starch grains in atypical tissues, namely, stomata and parenchyma cells, were observed in species of the genus Duchesnea. However, no morphological alteration was observed in Fragaria species or in P. tucumanensis, which did not show any apparent interaction with the isolate of C. gloeosporioides. Oxidative burst was evaluated in leaf tissues with the fluorescent probe DCFH-DA (2,7-Dichlorofluorescein diacetate) in susceptible and tolerant genotypes. Results showed that tolerant species present a noticeable accumulation of H2O2 whereas susceptible species did not. These results have led us to the conclusion that the changes observed were correlated with the resistance to disease.
Fil: Pardo, Esteban Mariano. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Tucumán. Instituto Superior de Investigaciones Biológicas. Universidad Nacional de Tucumán. Instituto Superior de Investigaciones Biológicas; Argentina. Universidad Nacional de Tucumán. Facultad de Ciencias Naturales e Instituto Miguel Lillo. Departamento de Biología. Cátedra de Anatomía Vegetal; Argentina
Fil: Grellet Bournonville, Carlos Froilan. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Tucumán. Instituto Superior de Investigaciones Biológicas. Universidad Nacional de Tucumán. Instituto Superior de Investigaciones Biológicas; Argentina
Fil: Salazar, Sergio Miguel. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Tucumán. Instituto Superior de Investigaciones Biológicas. Universidad Nacional de Tucumán. Instituto Superior de Investigaciones Biológicas; Argentina. Universidad Nacional de Tucumán. Facultad de Agronomía y Zootecnia; Argentina
Fil: Castagnaro, Atilio Pedro. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Tucumán. Instituto Superior de Investigaciones Biológicas. Universidad Nacional de Tucumán. Instituto Superior de Investigaciones Biológicas; Argentina
Fil: Diaz Ricci, Juan Carlos. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Tucumán. Instituto Superior de Investigaciones Biológicas. Universidad Nacional de Tucumán. Instituto Superior de Investigaciones Biológicas; Argentina
Fil: Arias, Marta Eugenia. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Tucumán. Instituto Superior de Investigaciones Biológicas. Universidad Nacional de Tucumán. Instituto Superior de Investigaciones Biológicas; Argentina. Universidad Nacional de Tucumán. Facultad de Ciencias Naturales e Instituto Miguel Lillo. Departamento de Biología. Cátedra de Anatomía Vegetal; Argentina
Materia
Duchesnea indica
Potentilla tucumanensis
Fragaria vesca
Colletotrichum
Nivel de accesibilidad
acceso abierto
Condiciones de uso
https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
Repositorio
CONICET Digital (CONICET)
Institución
Consejo Nacional de Investigaciones Científicas y Técnicas
OAI Identificador
oai:ri.conicet.gov.ar:11336/272299
Acceder
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network_name_str CONICET Digital (CONICET)
spelling Histopathology of the resistance to Colletotrichum gloeosporioides of wild strawberries and species related to commercial strawberryPardo, Esteban MarianoGrellet Bournonville, Carlos FroilanSalazar, Sergio MiguelCastagnaro, Atilio PedroDiaz Ricci, Juan CarlosArias, Marta EugeniaDuchesnea indicaPotentilla tucumanensisFragaria vescaColletotrichumhttps://purl.org/becyt/ford/1.6https://purl.org/becyt/ford/1Three species of wild strawberries, as well as three related species, were tested against virulent isolate of Colletotrichum gloeosporioides. The purpose of this study was to assess genetic resources exhibiting resistance to anthracnose. The results of inoculation experiments showed that the species Fragaria vesca, Fragaria virginiana and Fragaria chiloensis were susceptible to fungi; whereas species Duchesnea indica, Duchesnea chrysantha and Potentilla tucumanensis showed higher levels of resistance. A histopathological study revealed a different response between susceptible and tolerant species. A significant thickening of the cell wall in epidermal cells, changes of stomata and mesophyll cells, the presence of idioblastic cells with unknown content and the accumulation of large starch grains in atypical tissues, namely, stomata and parenchyma cells, were observed in species of the genus Duchesnea. However, no morphological alteration was observed in Fragaria species or in P. tucumanensis, which did not show any apparent interaction with the isolate of C. gloeosporioides. Oxidative burst was evaluated in leaf tissues with the fluorescent probe DCFH-DA (2,7-Dichlorofluorescein diacetate) in susceptible and tolerant genotypes. Results showed that tolerant species present a noticeable accumulation of H2O2 whereas susceptible species did not. These results have led us to the conclusion that the changes observed were correlated with the resistance to disease.Fil: Pardo, Esteban Mariano. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Tucumán. Instituto Superior de Investigaciones Biológicas. Universidad Nacional de Tucumán. Instituto Superior de Investigaciones Biológicas; Argentina. Universidad Nacional de Tucumán. Facultad de Ciencias Naturales e Instituto Miguel Lillo. Departamento de Biología. Cátedra de Anatomía Vegetal; ArgentinaFil: Grellet Bournonville, Carlos Froilan. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Tucumán. Instituto Superior de Investigaciones Biológicas. Universidad Nacional de Tucumán. Instituto Superior de Investigaciones Biológicas; ArgentinaFil: Salazar, Sergio Miguel. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Tucumán. Instituto Superior de Investigaciones Biológicas. Universidad Nacional de Tucumán. Instituto Superior de Investigaciones Biológicas; Argentina. Universidad Nacional de Tucumán. Facultad de Agronomía y Zootecnia; ArgentinaFil: Castagnaro, Atilio Pedro. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Tucumán. Instituto Superior de Investigaciones Biológicas. Universidad Nacional de Tucumán. Instituto Superior de Investigaciones Biológicas; ArgentinaFil: Diaz Ricci, Juan Carlos. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Tucumán. Instituto Superior de Investigaciones Biológicas. Universidad Nacional de Tucumán. Instituto Superior de Investigaciones Biológicas; ArgentinaFil: Arias, Marta Eugenia. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Tucumán. Instituto Superior de Investigaciones Biológicas. Universidad Nacional de Tucumán. Instituto Superior de Investigaciones Biológicas; Argentina. Universidad Nacional de Tucumán. Facultad de Ciencias Naturales e Instituto Miguel Lillo. Departamento de Biología. Cátedra de Anatomía Vegetal; ArgentinaSouthern Cross Publishing2012-07info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionhttp://purl.org/coar/resource_type/c_6501info:ar-repo/semantics/articuloapplication/pdfapplication/pdfapplication/pdfapplication/pdfapplication/pdfapplication/pdfapplication/pdfhttp://hdl.handle.net/11336/272299Pardo, Esteban Mariano; Grellet Bournonville, Carlos Froilan; Salazar, Sergio Miguel; Castagnaro, Atilio Pedro; Diaz Ricci, Juan Carlos; et al.; Histopathology of the resistance to Colletotrichum gloeosporioides of wild strawberries and species related to commercial strawberry; Southern Cross Publishing; Australian Journal of Crop Science; 6; 7; 7-2012; 1147-11531835-26931835-2707CONICET DigitalCONICETenginfo:eu-repo/semantics/altIdentifier/url/https://www.cropj.com/pardo_6_7_2012_1147_1153.pdfinfo:eu-repo/semantics/openAccesshttps://creativecommons.org/licenses/by-nc-sa/2.5/ar/reponame:CONICET Digital (CONICET)instname:Consejo Nacional de Investigaciones Científicas y Técnicas2025-10-15T15:37:07Zoai:ri.conicet.gov.ar:11336/272299instacron:CONICETInstitucionalhttp://ri.conicet.gov.ar/Organismo científico-tecnológicoNo correspondehttp://ri.conicet.gov.ar/oai/requestdasensio@conicet.gov.ar; lcarlino@conicet.gov.arArgentinaNo correspondeNo correspondeNo correspondeopendoar:34982025-10-15 15:37:07.497CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicasfalse
dc.title.none.fl_str_mv Histopathology of the resistance to Colletotrichum gloeosporioides of wild strawberries and species related to commercial strawberry
title Histopathology of the resistance to Colletotrichum gloeosporioides of wild strawberries and species related to commercial strawberry
spellingShingle Histopathology of the resistance to Colletotrichum gloeosporioides of wild strawberries and species related to commercial strawberry
Pardo, Esteban Mariano
Duchesnea indica
Potentilla tucumanensis
Fragaria vesca
Colletotrichum
title_short Histopathology of the resistance to Colletotrichum gloeosporioides of wild strawberries and species related to commercial strawberry
title_full Histopathology of the resistance to Colletotrichum gloeosporioides of wild strawberries and species related to commercial strawberry
title_fullStr Histopathology of the resistance to Colletotrichum gloeosporioides of wild strawberries and species related to commercial strawberry
title_full_unstemmed Histopathology of the resistance to Colletotrichum gloeosporioides of wild strawberries and species related to commercial strawberry
title_sort Histopathology of the resistance to Colletotrichum gloeosporioides of wild strawberries and species related to commercial strawberry
dc.creator.none.fl_str_mv Pardo, Esteban Mariano
Grellet Bournonville, Carlos Froilan
Salazar, Sergio Miguel
Castagnaro, Atilio Pedro
Diaz Ricci, Juan Carlos
Arias, Marta Eugenia
author Pardo, Esteban Mariano
author_facet Pardo, Esteban Mariano
Grellet Bournonville, Carlos Froilan
Salazar, Sergio Miguel
Castagnaro, Atilio Pedro
Diaz Ricci, Juan Carlos
Arias, Marta Eugenia
author_role author
author2 Grellet Bournonville, Carlos Froilan
Salazar, Sergio Miguel
Castagnaro, Atilio Pedro
Diaz Ricci, Juan Carlos
Arias, Marta Eugenia
author2_role author
author
author
author
author
dc.subject.none.fl_str_mv Duchesnea indica
Potentilla tucumanensis
Fragaria vesca
Colletotrichum
topic Duchesnea indica
Potentilla tucumanensis
Fragaria vesca
Colletotrichum
purl_subject.fl_str_mv https://purl.org/becyt/ford/1.6
https://purl.org/becyt/ford/1
dc.description.none.fl_txt_mv Three species of wild strawberries, as well as three related species, were tested against virulent isolate of Colletotrichum gloeosporioides. The purpose of this study was to assess genetic resources exhibiting resistance to anthracnose. The results of inoculation experiments showed that the species Fragaria vesca, Fragaria virginiana and Fragaria chiloensis were susceptible to fungi; whereas species Duchesnea indica, Duchesnea chrysantha and Potentilla tucumanensis showed higher levels of resistance. A histopathological study revealed a different response between susceptible and tolerant species. A significant thickening of the cell wall in epidermal cells, changes of stomata and mesophyll cells, the presence of idioblastic cells with unknown content and the accumulation of large starch grains in atypical tissues, namely, stomata and parenchyma cells, were observed in species of the genus Duchesnea. However, no morphological alteration was observed in Fragaria species or in P. tucumanensis, which did not show any apparent interaction with the isolate of C. gloeosporioides. Oxidative burst was evaluated in leaf tissues with the fluorescent probe DCFH-DA (2,7-Dichlorofluorescein diacetate) in susceptible and tolerant genotypes. Results showed that tolerant species present a noticeable accumulation of H2O2 whereas susceptible species did not. These results have led us to the conclusion that the changes observed were correlated with the resistance to disease.
Fil: Pardo, Esteban Mariano. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Tucumán. Instituto Superior de Investigaciones Biológicas. Universidad Nacional de Tucumán. Instituto Superior de Investigaciones Biológicas; Argentina. Universidad Nacional de Tucumán. Facultad de Ciencias Naturales e Instituto Miguel Lillo. Departamento de Biología. Cátedra de Anatomía Vegetal; Argentina
Fil: Grellet Bournonville, Carlos Froilan. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Tucumán. Instituto Superior de Investigaciones Biológicas. Universidad Nacional de Tucumán. Instituto Superior de Investigaciones Biológicas; Argentina
Fil: Salazar, Sergio Miguel. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Tucumán. Instituto Superior de Investigaciones Biológicas. Universidad Nacional de Tucumán. Instituto Superior de Investigaciones Biológicas; Argentina. Universidad Nacional de Tucumán. Facultad de Agronomía y Zootecnia; Argentina
Fil: Castagnaro, Atilio Pedro. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Tucumán. Instituto Superior de Investigaciones Biológicas. Universidad Nacional de Tucumán. Instituto Superior de Investigaciones Biológicas; Argentina
Fil: Diaz Ricci, Juan Carlos. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Tucumán. Instituto Superior de Investigaciones Biológicas. Universidad Nacional de Tucumán. Instituto Superior de Investigaciones Biológicas; Argentina
Fil: Arias, Marta Eugenia. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Tucumán. Instituto Superior de Investigaciones Biológicas. Universidad Nacional de Tucumán. Instituto Superior de Investigaciones Biológicas; Argentina. Universidad Nacional de Tucumán. Facultad de Ciencias Naturales e Instituto Miguel Lillo. Departamento de Biología. Cátedra de Anatomía Vegetal; Argentina
description Three species of wild strawberries, as well as three related species, were tested against virulent isolate of Colletotrichum gloeosporioides. The purpose of this study was to assess genetic resources exhibiting resistance to anthracnose. The results of inoculation experiments showed that the species Fragaria vesca, Fragaria virginiana and Fragaria chiloensis were susceptible to fungi; whereas species Duchesnea indica, Duchesnea chrysantha and Potentilla tucumanensis showed higher levels of resistance. A histopathological study revealed a different response between susceptible and tolerant species. A significant thickening of the cell wall in epidermal cells, changes of stomata and mesophyll cells, the presence of idioblastic cells with unknown content and the accumulation of large starch grains in atypical tissues, namely, stomata and parenchyma cells, were observed in species of the genus Duchesnea. However, no morphological alteration was observed in Fragaria species or in P. tucumanensis, which did not show any apparent interaction with the isolate of C. gloeosporioides. Oxidative burst was evaluated in leaf tissues with the fluorescent probe DCFH-DA (2,7-Dichlorofluorescein diacetate) in susceptible and tolerant genotypes. Results showed that tolerant species present a noticeable accumulation of H2O2 whereas susceptible species did not. These results have led us to the conclusion that the changes observed were correlated with the resistance to disease.
publishDate 2012
dc.date.none.fl_str_mv 2012-07
dc.type.none.fl_str_mv info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
http://purl.org/coar/resource_type/c_6501
info:ar-repo/semantics/articulo
format article
status_str publishedVersion
dc.identifier.none.fl_str_mv http://hdl.handle.net/11336/272299
Pardo, Esteban Mariano; Grellet Bournonville, Carlos Froilan; Salazar, Sergio Miguel; Castagnaro, Atilio Pedro; Diaz Ricci, Juan Carlos; et al.; Histopathology of the resistance to Colletotrichum gloeosporioides of wild strawberries and species related to commercial strawberry; Southern Cross Publishing; Australian Journal of Crop Science; 6; 7; 7-2012; 1147-1153
1835-2693
1835-2707
CONICET Digital
CONICET
url http://hdl.handle.net/11336/272299
identifier_str_mv Pardo, Esteban Mariano; Grellet Bournonville, Carlos Froilan; Salazar, Sergio Miguel; Castagnaro, Atilio Pedro; Diaz Ricci, Juan Carlos; et al.; Histopathology of the resistance to Colletotrichum gloeosporioides of wild strawberries and species related to commercial strawberry; Southern Cross Publishing; Australian Journal of Crop Science; 6; 7; 7-2012; 1147-1153
1835-2693
1835-2707
CONICET Digital
CONICET
dc.language.none.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv info:eu-repo/semantics/altIdentifier/url/https://www.cropj.com/pardo_6_7_2012_1147_1153.pdf
dc.rights.none.fl_str_mv info:eu-repo/semantics/openAccess
https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
eu_rights_str_mv openAccess
rights_invalid_str_mv https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
dc.format.none.fl_str_mv application/pdf
application/pdf
application/pdf
application/pdf
application/pdf
application/pdf
application/pdf
dc.publisher.none.fl_str_mv Southern Cross Publishing
publisher.none.fl_str_mv Southern Cross Publishing
dc.source.none.fl_str_mv reponame:CONICET Digital (CONICET)
instname:Consejo Nacional de Investigaciones Científicas y Técnicas
reponame_str CONICET Digital (CONICET)
collection CONICET Digital (CONICET)
instname_str Consejo Nacional de Investigaciones Científicas y Técnicas
repository.name.fl_str_mv CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicas
repository.mail.fl_str_mv dasensio@conicet.gov.ar; lcarlino@conicet.gov.ar
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score 13.22299

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