Proteomic analysis of the parasitic Cnidarian Ceratonova shasta (Cnidaria: Myxozoa) reveals diverse roles of actin in motility and spore formation

Autores
Brekhman, Vera; Ofek Lalzar, Maya; Atkinson, Stephen D.; Alama Bermejo, Gema; Maor Landaw, Keren; Malik, Assaf; Bartholomew, Jerri; Lotan, Tamar
Año de publicación
2021
Idioma
inglés
Tipo de recurso
artículo
Estado
versión publicada
Descripción
Myxozoans are widely distributed aquatic obligate endoparasites that were recently recognized as belonging within the phylum Cnidaria. They have complex life cycles with waterborne transmission stages: resistant, infectious spores that are unique to myxozoans. However, little is known about the processes that give rise to these transmission stages. To understand the molecular underpinnings of spore formation, we conducted proteomics on Ceratonova shasta, a highly pathogenic myxozoan that causes severe mortalities in wild and hatchery-reared salmonid fishes. We compared proteomic profiles between developmental stages from inside the fish host, and the mature myxospore, which is released into the water where it drifts passively, ready to infect the next host. We found that C. shasta contains 2,123 proteins; representing the first proteomic catalog of a myxozoan myxospore. Analysis of proteins differentially expressed between developing and mature spore stages uncovered processes that are active during spore formation. Our data highlight dynamic changes in the actin cytoskeleton, which provides myxozoan developmental stages with mobility through lamellipodia and filopodia, whereas in the mature myxospore the actin network supports F-actin stabilization that reinforces the transmission stage. These findings provide molecular insight into the myxozoan life cycle stages and, particularly, into the process of sporogenesis.
Fil: Brekhman, Vera. University Of Haifa; Israel
Fil: Ofek Lalzar, Maya. University Of Haifa; Israel
Fil: Atkinson, Stephen D.. State University of Oregon; Estados Unidos
Fil: Alama Bermejo, Gema. Universidad Nacional del Comahue. Centro de Investigación Aplicada y Transferencia Tecnológica en Recursos Marinos "Almirante Storni". - Provincia de Río Negro. Ministerio de Agricultura, Ganadería y Pesca. Centro de Investigación Aplicada y Transferencia Tecnológica en Recursos Marinos "Almirante Storni". Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet Centro Nacional Patagónico. Centro de Investigación Aplicada y Transferencia Tecnológica en Recursos Marinos "Almirante Storni"; Argentina. State University of Oregon; Estados Unidos. Institute of Parasitology. Biology Centre of the Czech Academy of Sciences; República Checa
Fil: Maor Landaw, Keren. University Of Haifa; Israel
Fil: Malik, Assaf. University Of Haifa; Israel
Fil: Bartholomew, Jerri. State University of Oregon; Estados Unidos
Fil: Lotan, Tamar. University Of Haifa; Israel
Materia
ACTIN
CNIDARIA
CYTOSKELETON
MYXOZOA
PARASITE
SPOROGENESIS
Nivel de accesibilidad
acceso abierto
Condiciones de uso
https://creativecommons.org/licenses/by/2.5/ar/
Repositorio
CONICET Digital (CONICET)
Institución
Consejo Nacional de Investigaciones Científicas y Técnicas
OAI Identificador
oai:ri.conicet.gov.ar:11336/163531
Acceder
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oai_identifier_str oai:ri.conicet.gov.ar:11336/163531
network_acronym_str CONICETDig
repository_id_str 3498
network_name_str CONICET Digital (CONICET)
spelling Proteomic analysis of the parasitic Cnidarian Ceratonova shasta (Cnidaria: Myxozoa) reveals diverse roles of actin in motility and spore formationBrekhman, VeraOfek Lalzar, MayaAtkinson, Stephen D.Alama Bermejo, GemaMaor Landaw, KerenMalik, AssafBartholomew, JerriLotan, TamarACTINCNIDARIACYTOSKELETONMYXOZOAPARASITESPOROGENESIShttps://purl.org/becyt/ford/1.6https://purl.org/becyt/ford/1Myxozoans are widely distributed aquatic obligate endoparasites that were recently recognized as belonging within the phylum Cnidaria. They have complex life cycles with waterborne transmission stages: resistant, infectious spores that are unique to myxozoans. However, little is known about the processes that give rise to these transmission stages. To understand the molecular underpinnings of spore formation, we conducted proteomics on Ceratonova shasta, a highly pathogenic myxozoan that causes severe mortalities in wild and hatchery-reared salmonid fishes. We compared proteomic profiles between developmental stages from inside the fish host, and the mature myxospore, which is released into the water where it drifts passively, ready to infect the next host. We found that C. shasta contains 2,123 proteins; representing the first proteomic catalog of a myxozoan myxospore. Analysis of proteins differentially expressed between developing and mature spore stages uncovered processes that are active during spore formation. Our data highlight dynamic changes in the actin cytoskeleton, which provides myxozoan developmental stages with mobility through lamellipodia and filopodia, whereas in the mature myxospore the actin network supports F-actin stabilization that reinforces the transmission stage. These findings provide molecular insight into the myxozoan life cycle stages and, particularly, into the process of sporogenesis.Fil: Brekhman, Vera. University Of Haifa; IsraelFil: Ofek Lalzar, Maya. University Of Haifa; IsraelFil: Atkinson, Stephen D.. State University of Oregon; Estados UnidosFil: Alama Bermejo, Gema. Universidad Nacional del Comahue. Centro de Investigación Aplicada y Transferencia Tecnológica en Recursos Marinos "Almirante Storni". - Provincia de Río Negro. Ministerio de Agricultura, Ganadería y Pesca. Centro de Investigación Aplicada y Transferencia Tecnológica en Recursos Marinos "Almirante Storni". Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet Centro Nacional Patagónico. Centro de Investigación Aplicada y Transferencia Tecnológica en Recursos Marinos "Almirante Storni"; Argentina. State University of Oregon; Estados Unidos. Institute of Parasitology. Biology Centre of the Czech Academy of Sciences; República ChecaFil: Maor Landaw, Keren. University Of Haifa; IsraelFil: Malik, Assaf. University Of Haifa; IsraelFil: Bartholomew, Jerri. State University of Oregon; Estados UnidosFil: Lotan, Tamar. University Of Haifa; IsraelFrontiers Media2021-04info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionhttp://purl.org/coar/resource_type/c_6501info:ar-repo/semantics/articuloapplication/pdfapplication/pdfhttp://hdl.handle.net/11336/163531Brekhman, Vera; Ofek Lalzar, Maya; Atkinson, Stephen D.; Alama Bermejo, Gema; Maor Landaw, Keren; et al.; Proteomic analysis of the parasitic Cnidarian Ceratonova shasta (Cnidaria: Myxozoa) reveals diverse roles of actin in motility and spore formation; Frontiers Media; Frontiers In Marine Science; 8; 4-2021; 1-82296-7745CONICET DigitalCONICETenginfo:eu-repo/semantics/altIdentifier/url/https://www.frontiersin.org/articles/10.3389/fmars.2021.632700/fullinfo:eu-repo/semantics/altIdentifier/doi/10.3389/fmars.2021.632700info:eu-repo/semantics/openAccesshttps://creativecommons.org/licenses/by/2.5/ar/reponame:CONICET Digital (CONICET)instname:Consejo Nacional de Investigaciones Científicas y Técnicas2025-09-29T09:47:44Zoai:ri.conicet.gov.ar:11336/163531instacron:CONICETInstitucionalhttp://ri.conicet.gov.ar/Organismo científico-tecnológicoNo correspondehttp://ri.conicet.gov.ar/oai/requestdasensio@conicet.gov.ar; lcarlino@conicet.gov.arArgentinaNo correspondeNo correspondeNo correspondeopendoar:34982025-09-29 09:47:44.614CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicasfalse
dc.title.none.fl_str_mv Proteomic analysis of the parasitic Cnidarian Ceratonova shasta (Cnidaria: Myxozoa) reveals diverse roles of actin in motility and spore formation
title Proteomic analysis of the parasitic Cnidarian Ceratonova shasta (Cnidaria: Myxozoa) reveals diverse roles of actin in motility and spore formation
spellingShingle Proteomic analysis of the parasitic Cnidarian Ceratonova shasta (Cnidaria: Myxozoa) reveals diverse roles of actin in motility and spore formation
Brekhman, Vera
ACTIN
CNIDARIA
CYTOSKELETON
MYXOZOA
PARASITE
SPOROGENESIS
title_short Proteomic analysis of the parasitic Cnidarian Ceratonova shasta (Cnidaria: Myxozoa) reveals diverse roles of actin in motility and spore formation
title_full Proteomic analysis of the parasitic Cnidarian Ceratonova shasta (Cnidaria: Myxozoa) reveals diverse roles of actin in motility and spore formation
title_fullStr Proteomic analysis of the parasitic Cnidarian Ceratonova shasta (Cnidaria: Myxozoa) reveals diverse roles of actin in motility and spore formation
title_full_unstemmed Proteomic analysis of the parasitic Cnidarian Ceratonova shasta (Cnidaria: Myxozoa) reveals diverse roles of actin in motility and spore formation
title_sort Proteomic analysis of the parasitic Cnidarian Ceratonova shasta (Cnidaria: Myxozoa) reveals diverse roles of actin in motility and spore formation
dc.creator.none.fl_str_mv Brekhman, Vera
Ofek Lalzar, Maya
Atkinson, Stephen D.
Alama Bermejo, Gema
Maor Landaw, Keren
Malik, Assaf
Bartholomew, Jerri
Lotan, Tamar
author Brekhman, Vera
author_facet Brekhman, Vera
Ofek Lalzar, Maya
Atkinson, Stephen D.
Alama Bermejo, Gema
Maor Landaw, Keren
Malik, Assaf
Bartholomew, Jerri
Lotan, Tamar
author_role author
author2 Ofek Lalzar, Maya
Atkinson, Stephen D.
Alama Bermejo, Gema
Maor Landaw, Keren
Malik, Assaf
Bartholomew, Jerri
Lotan, Tamar
author2_role author
author
author
author
author
author
author
dc.subject.none.fl_str_mv ACTIN
CNIDARIA
CYTOSKELETON
MYXOZOA
PARASITE
SPOROGENESIS
topic ACTIN
CNIDARIA
CYTOSKELETON
MYXOZOA
PARASITE
SPOROGENESIS
purl_subject.fl_str_mv https://purl.org/becyt/ford/1.6
https://purl.org/becyt/ford/1
dc.description.none.fl_txt_mv Myxozoans are widely distributed aquatic obligate endoparasites that were recently recognized as belonging within the phylum Cnidaria. They have complex life cycles with waterborne transmission stages: resistant, infectious spores that are unique to myxozoans. However, little is known about the processes that give rise to these transmission stages. To understand the molecular underpinnings of spore formation, we conducted proteomics on Ceratonova shasta, a highly pathogenic myxozoan that causes severe mortalities in wild and hatchery-reared salmonid fishes. We compared proteomic profiles between developmental stages from inside the fish host, and the mature myxospore, which is released into the water where it drifts passively, ready to infect the next host. We found that C. shasta contains 2,123 proteins; representing the first proteomic catalog of a myxozoan myxospore. Analysis of proteins differentially expressed between developing and mature spore stages uncovered processes that are active during spore formation. Our data highlight dynamic changes in the actin cytoskeleton, which provides myxozoan developmental stages with mobility through lamellipodia and filopodia, whereas in the mature myxospore the actin network supports F-actin stabilization that reinforces the transmission stage. These findings provide molecular insight into the myxozoan life cycle stages and, particularly, into the process of sporogenesis.
Fil: Brekhman, Vera. University Of Haifa; Israel
Fil: Ofek Lalzar, Maya. University Of Haifa; Israel
Fil: Atkinson, Stephen D.. State University of Oregon; Estados Unidos
Fil: Alama Bermejo, Gema. Universidad Nacional del Comahue. Centro de Investigación Aplicada y Transferencia Tecnológica en Recursos Marinos "Almirante Storni". - Provincia de Río Negro. Ministerio de Agricultura, Ganadería y Pesca. Centro de Investigación Aplicada y Transferencia Tecnológica en Recursos Marinos "Almirante Storni". Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet Centro Nacional Patagónico. Centro de Investigación Aplicada y Transferencia Tecnológica en Recursos Marinos "Almirante Storni"; Argentina. State University of Oregon; Estados Unidos. Institute of Parasitology. Biology Centre of the Czech Academy of Sciences; República Checa
Fil: Maor Landaw, Keren. University Of Haifa; Israel
Fil: Malik, Assaf. University Of Haifa; Israel
Fil: Bartholomew, Jerri. State University of Oregon; Estados Unidos
Fil: Lotan, Tamar. University Of Haifa; Israel
description Myxozoans are widely distributed aquatic obligate endoparasites that were recently recognized as belonging within the phylum Cnidaria. They have complex life cycles with waterborne transmission stages: resistant, infectious spores that are unique to myxozoans. However, little is known about the processes that give rise to these transmission stages. To understand the molecular underpinnings of spore formation, we conducted proteomics on Ceratonova shasta, a highly pathogenic myxozoan that causes severe mortalities in wild and hatchery-reared salmonid fishes. We compared proteomic profiles between developmental stages from inside the fish host, and the mature myxospore, which is released into the water where it drifts passively, ready to infect the next host. We found that C. shasta contains 2,123 proteins; representing the first proteomic catalog of a myxozoan myxospore. Analysis of proteins differentially expressed between developing and mature spore stages uncovered processes that are active during spore formation. Our data highlight dynamic changes in the actin cytoskeleton, which provides myxozoan developmental stages with mobility through lamellipodia and filopodia, whereas in the mature myxospore the actin network supports F-actin stabilization that reinforces the transmission stage. These findings provide molecular insight into the myxozoan life cycle stages and, particularly, into the process of sporogenesis.
publishDate 2021
dc.date.none.fl_str_mv 2021-04
dc.type.none.fl_str_mv info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
http://purl.org/coar/resource_type/c_6501
info:ar-repo/semantics/articulo
format article
status_str publishedVersion
dc.identifier.none.fl_str_mv http://hdl.handle.net/11336/163531
Brekhman, Vera; Ofek Lalzar, Maya; Atkinson, Stephen D.; Alama Bermejo, Gema; Maor Landaw, Keren; et al.; Proteomic analysis of the parasitic Cnidarian Ceratonova shasta (Cnidaria: Myxozoa) reveals diverse roles of actin in motility and spore formation; Frontiers Media; Frontiers In Marine Science; 8; 4-2021; 1-8
2296-7745
CONICET Digital
CONICET
url http://hdl.handle.net/11336/163531
identifier_str_mv Brekhman, Vera; Ofek Lalzar, Maya; Atkinson, Stephen D.; Alama Bermejo, Gema; Maor Landaw, Keren; et al.; Proteomic analysis of the parasitic Cnidarian Ceratonova shasta (Cnidaria: Myxozoa) reveals diverse roles of actin in motility and spore formation; Frontiers Media; Frontiers In Marine Science; 8; 4-2021; 1-8
2296-7745
CONICET Digital
CONICET
dc.language.none.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv info:eu-repo/semantics/altIdentifier/url/https://www.frontiersin.org/articles/10.3389/fmars.2021.632700/full
info:eu-repo/semantics/altIdentifier/doi/10.3389/fmars.2021.632700
dc.rights.none.fl_str_mv info:eu-repo/semantics/openAccess
https://creativecommons.org/licenses/by/2.5/ar/
eu_rights_str_mv openAccess
rights_invalid_str_mv https://creativecommons.org/licenses/by/2.5/ar/
dc.format.none.fl_str_mv application/pdf
application/pdf
dc.publisher.none.fl_str_mv Frontiers Media
publisher.none.fl_str_mv Frontiers Media
dc.source.none.fl_str_mv reponame:CONICET Digital (CONICET)
instname:Consejo Nacional de Investigaciones Científicas y Técnicas
reponame_str CONICET Digital (CONICET)
collection CONICET Digital (CONICET)
instname_str Consejo Nacional de Investigaciones Científicas y Técnicas
repository.name.fl_str_mv CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicas
repository.mail.fl_str_mv dasensio@conicet.gov.ar; lcarlino@conicet.gov.ar
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score 13.070432

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