3ß-hydroxysteroid dehydrogenase expression in rat spinal cord

Autores
Coirini, Hector; Gouezou, M.; Liere, P.; Delespierre, B.; Pianos, A.; Eychenne, B.; Schumacher, M.; Guennoun, R..
Año de publicación
2002
Idioma
inglés
Tipo de recurso
artículo
Estado
versión publicada
Descripción
In adult male rats, 3beta-hydroxysteroid dehydrogenase/delta5-delta4-isomerase (3beta-HSD) expressing cells were identified in the spinal cord from the cervical to the sacral segments. An in situ hybridization study, using an oligonucleotide common to the four known isoforms of rat 3beta-HSD, revealed its mRNA in gray matter. Measurements of optical densities in autoradiograms showed the following regional distribution: dorsal horn (layers I-III) > central canal (layer X) > or = ventral horn (layers VIII-IX) > ventral funiculus = lateral funiculus. At the cellular level, the number of grains was higher on the large motoneurons than on small neurons of the dorsal horn, but the grain density per cell was similar. Further evidence for the expression of 3beta-HSD in the spinal cord was obtained by western blot analysis, which revealed an immunoreactive protein of approximately 45 kDa in the dorsal and ventral parts of the spinal cord. Castration and adrenalectomy did not influence the expression of 3beta-HSD mRNA and protein. Gas chromatography/mass spectrometry measurements showed higher levels of pregnenolone and progesterone in the spinal cord than in the plasma. After castration and adrenalectomy, their levels remained elevated in the spinal cord, suggesting that these neurosteroids may be synthesized locally. The wide distribution of 3beta-HSD, and the high levels of pregnenolone and progesterone in the spinal cord even after castration and adrenalectomy, strongly suggest a potential endogenous production of progesterone and an important signalling function of this steroid in the spinal cord.
Fil: Coirini, Hector. Consejo Nacional de Investigaciones Científicas y Técnicas. Instituto de Biología y Medicina Experimental. Fundación de Instituto de Biología y Medicina Experimental. Instituto de Biología y Medicina Experimental; Argentina. Inserm; Francia
Fil: Gouezou, M.. Inserm; Francia
Fil: Liere, P.. Inserm; Francia
Fil: Delespierre, B.. Inserm; Francia
Fil: Pianos, A.. Inserm; Francia
Fil: Eychenne, B.. Inserm; Francia
Fil: Schumacher, M.. Inserm; Francia
Fil: Guennoun, R... Inserm; Francia
Materia
Neurosteroids
3b-Hydroxysteroid Dehydrogenase
Spinal Cord
In Situ Hybridization
Nivel de accesibilidad
acceso abierto
Condiciones de uso
https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
Repositorio
CONICET Digital (CONICET)
Institución
Consejo Nacional de Investigaciones Científicas y Técnicas
OAI Identificador
oai:ri.conicet.gov.ar:11336/30717
Acceder
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oai_identifier_str oai:ri.conicet.gov.ar:11336/30717
network_acronym_str CONICETDig
repository_id_str 3498
network_name_str CONICET Digital (CONICET)
spelling 3ß-hydroxysteroid dehydrogenase expression in rat spinal cordCoirini, HectorGouezou, M.Liere, P.Delespierre, B.Pianos, A.Eychenne, B.Schumacher, M.Guennoun, R..Neurosteroids3b-Hydroxysteroid DehydrogenaseSpinal CordIn Situ Hybridizationhttps://purl.org/becyt/ford/3.1https://purl.org/becyt/ford/3https://purl.org/becyt/ford/3.1https://purl.org/becyt/ford/3In adult male rats, 3beta-hydroxysteroid dehydrogenase/delta5-delta4-isomerase (3beta-HSD) expressing cells were identified in the spinal cord from the cervical to the sacral segments. An in situ hybridization study, using an oligonucleotide common to the four known isoforms of rat 3beta-HSD, revealed its mRNA in gray matter. Measurements of optical densities in autoradiograms showed the following regional distribution: dorsal horn (layers I-III) > central canal (layer X) > or = ventral horn (layers VIII-IX) > ventral funiculus = lateral funiculus. At the cellular level, the number of grains was higher on the large motoneurons than on small neurons of the dorsal horn, but the grain density per cell was similar. Further evidence for the expression of 3beta-HSD in the spinal cord was obtained by western blot analysis, which revealed an immunoreactive protein of approximately 45 kDa in the dorsal and ventral parts of the spinal cord. Castration and adrenalectomy did not influence the expression of 3beta-HSD mRNA and protein. Gas chromatography/mass spectrometry measurements showed higher levels of pregnenolone and progesterone in the spinal cord than in the plasma. After castration and adrenalectomy, their levels remained elevated in the spinal cord, suggesting that these neurosteroids may be synthesized locally. The wide distribution of 3beta-HSD, and the high levels of pregnenolone and progesterone in the spinal cord even after castration and adrenalectomy, strongly suggest a potential endogenous production of progesterone and an important signalling function of this steroid in the spinal cord.Fil: Coirini, Hector. Consejo Nacional de Investigaciones Científicas y Técnicas. Instituto de Biología y Medicina Experimental. Fundación de Instituto de Biología y Medicina Experimental. Instituto de Biología y Medicina Experimental; Argentina. Inserm; FranciaFil: Gouezou, M.. Inserm; FranciaFil: Liere, P.. Inserm; FranciaFil: Delespierre, B.. Inserm; FranciaFil: Pianos, A.. Inserm; FranciaFil: Eychenne, B.. Inserm; FranciaFil: Schumacher, M.. Inserm; FranciaFil: Guennoun, R... Inserm; FranciaIBRO2002-09info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionhttp://purl.org/coar/resource_type/c_6501info:ar-repo/semantics/articuloapplication/pdfapplication/pdfhttp://hdl.handle.net/11336/30717Coirini, Hector; Gouezou, M.; Liere, P.; Delespierre, B.; Pianos, A.; et al.; 3ß-hydroxysteroid dehydrogenase expression in rat spinal cord; IBRO; Neuroscience; 113; 4; 9-2002; 883-8910306-4522CONICET DigitalCONICETenginfo:eu-repo/semantics/altIdentifier/url/http://www.sciencedirect.com/science/article/pii/S0306452202002245?via%3Dihubinfo:eu-repo/semantics/altIdentifier/doi/10.1016/S0306-4522(02)00224-5info:eu-repo/semantics/altIdentifier/pmid/12182894info:eu-repo/semantics/openAccesshttps://creativecommons.org/licenses/by-nc-sa/2.5/ar/reponame:CONICET Digital (CONICET)instname:Consejo Nacional de Investigaciones Científicas y Técnicas2025-09-03T09:58:57Zoai:ri.conicet.gov.ar:11336/30717instacron:CONICETInstitucionalhttp://ri.conicet.gov.ar/Organismo científico-tecnológicoNo correspondehttp://ri.conicet.gov.ar/oai/requestdasensio@conicet.gov.ar; lcarlino@conicet.gov.arArgentinaNo correspondeNo correspondeNo correspondeopendoar:34982025-09-03 09:58:57.793CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicasfalse
dc.title.none.fl_str_mv 3ß-hydroxysteroid dehydrogenase expression in rat spinal cord
title 3ß-hydroxysteroid dehydrogenase expression in rat spinal cord
spellingShingle 3ß-hydroxysteroid dehydrogenase expression in rat spinal cord
Coirini, Hector
Neurosteroids
3b-Hydroxysteroid Dehydrogenase
Spinal Cord
In Situ Hybridization
title_short 3ß-hydroxysteroid dehydrogenase expression in rat spinal cord
title_full 3ß-hydroxysteroid dehydrogenase expression in rat spinal cord
title_fullStr 3ß-hydroxysteroid dehydrogenase expression in rat spinal cord
title_full_unstemmed 3ß-hydroxysteroid dehydrogenase expression in rat spinal cord
title_sort 3ß-hydroxysteroid dehydrogenase expression in rat spinal cord
dc.creator.none.fl_str_mv Coirini, Hector
Gouezou, M.
Liere, P.
Delespierre, B.
Pianos, A.
Eychenne, B.
Schumacher, M.
Guennoun, R..
author Coirini, Hector
author_facet Coirini, Hector
Gouezou, M.
Liere, P.
Delespierre, B.
Pianos, A.
Eychenne, B.
Schumacher, M.
Guennoun, R..
author_role author
author2 Gouezou, M.
Liere, P.
Delespierre, B.
Pianos, A.
Eychenne, B.
Schumacher, M.
Guennoun, R..
author2_role author
author
author
author
author
author
author
dc.subject.none.fl_str_mv Neurosteroids
3b-Hydroxysteroid Dehydrogenase
Spinal Cord
In Situ Hybridization
topic Neurosteroids
3b-Hydroxysteroid Dehydrogenase
Spinal Cord
In Situ Hybridization
purl_subject.fl_str_mv https://purl.org/becyt/ford/3.1
https://purl.org/becyt/ford/3
https://purl.org/becyt/ford/3.1
https://purl.org/becyt/ford/3
dc.description.none.fl_txt_mv In adult male rats, 3beta-hydroxysteroid dehydrogenase/delta5-delta4-isomerase (3beta-HSD) expressing cells were identified in the spinal cord from the cervical to the sacral segments. An in situ hybridization study, using an oligonucleotide common to the four known isoforms of rat 3beta-HSD, revealed its mRNA in gray matter. Measurements of optical densities in autoradiograms showed the following regional distribution: dorsal horn (layers I-III) > central canal (layer X) > or = ventral horn (layers VIII-IX) > ventral funiculus = lateral funiculus. At the cellular level, the number of grains was higher on the large motoneurons than on small neurons of the dorsal horn, but the grain density per cell was similar. Further evidence for the expression of 3beta-HSD in the spinal cord was obtained by western blot analysis, which revealed an immunoreactive protein of approximately 45 kDa in the dorsal and ventral parts of the spinal cord. Castration and adrenalectomy did not influence the expression of 3beta-HSD mRNA and protein. Gas chromatography/mass spectrometry measurements showed higher levels of pregnenolone and progesterone in the spinal cord than in the plasma. After castration and adrenalectomy, their levels remained elevated in the spinal cord, suggesting that these neurosteroids may be synthesized locally. The wide distribution of 3beta-HSD, and the high levels of pregnenolone and progesterone in the spinal cord even after castration and adrenalectomy, strongly suggest a potential endogenous production of progesterone and an important signalling function of this steroid in the spinal cord.
Fil: Coirini, Hector. Consejo Nacional de Investigaciones Científicas y Técnicas. Instituto de Biología y Medicina Experimental. Fundación de Instituto de Biología y Medicina Experimental. Instituto de Biología y Medicina Experimental; Argentina. Inserm; Francia
Fil: Gouezou, M.. Inserm; Francia
Fil: Liere, P.. Inserm; Francia
Fil: Delespierre, B.. Inserm; Francia
Fil: Pianos, A.. Inserm; Francia
Fil: Eychenne, B.. Inserm; Francia
Fil: Schumacher, M.. Inserm; Francia
Fil: Guennoun, R... Inserm; Francia
description In adult male rats, 3beta-hydroxysteroid dehydrogenase/delta5-delta4-isomerase (3beta-HSD) expressing cells were identified in the spinal cord from the cervical to the sacral segments. An in situ hybridization study, using an oligonucleotide common to the four known isoforms of rat 3beta-HSD, revealed its mRNA in gray matter. Measurements of optical densities in autoradiograms showed the following regional distribution: dorsal horn (layers I-III) > central canal (layer X) > or = ventral horn (layers VIII-IX) > ventral funiculus = lateral funiculus. At the cellular level, the number of grains was higher on the large motoneurons than on small neurons of the dorsal horn, but the grain density per cell was similar. Further evidence for the expression of 3beta-HSD in the spinal cord was obtained by western blot analysis, which revealed an immunoreactive protein of approximately 45 kDa in the dorsal and ventral parts of the spinal cord. Castration and adrenalectomy did not influence the expression of 3beta-HSD mRNA and protein. Gas chromatography/mass spectrometry measurements showed higher levels of pregnenolone and progesterone in the spinal cord than in the plasma. After castration and adrenalectomy, their levels remained elevated in the spinal cord, suggesting that these neurosteroids may be synthesized locally. The wide distribution of 3beta-HSD, and the high levels of pregnenolone and progesterone in the spinal cord even after castration and adrenalectomy, strongly suggest a potential endogenous production of progesterone and an important signalling function of this steroid in the spinal cord.
publishDate 2002
dc.date.none.fl_str_mv 2002-09
dc.type.none.fl_str_mv info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
http://purl.org/coar/resource_type/c_6501
info:ar-repo/semantics/articulo
format article
status_str publishedVersion
dc.identifier.none.fl_str_mv http://hdl.handle.net/11336/30717
Coirini, Hector; Gouezou, M.; Liere, P.; Delespierre, B.; Pianos, A.; et al.; 3ß-hydroxysteroid dehydrogenase expression in rat spinal cord; IBRO; Neuroscience; 113; 4; 9-2002; 883-891
0306-4522
CONICET Digital
CONICET
url http://hdl.handle.net/11336/30717
identifier_str_mv Coirini, Hector; Gouezou, M.; Liere, P.; Delespierre, B.; Pianos, A.; et al.; 3ß-hydroxysteroid dehydrogenase expression in rat spinal cord; IBRO; Neuroscience; 113; 4; 9-2002; 883-891
0306-4522
CONICET Digital
CONICET
dc.language.none.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv info:eu-repo/semantics/altIdentifier/url/http://www.sciencedirect.com/science/article/pii/S0306452202002245?via%3Dihub
info:eu-repo/semantics/altIdentifier/doi/10.1016/S0306-4522(02)00224-5
info:eu-repo/semantics/altIdentifier/pmid/12182894
dc.rights.none.fl_str_mv info:eu-repo/semantics/openAccess
https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
eu_rights_str_mv openAccess
rights_invalid_str_mv https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
dc.format.none.fl_str_mv application/pdf
application/pdf
dc.publisher.none.fl_str_mv IBRO
publisher.none.fl_str_mv IBRO
dc.source.none.fl_str_mv reponame:CONICET Digital (CONICET)
instname:Consejo Nacional de Investigaciones Científicas y Técnicas
reponame_str CONICET Digital (CONICET)
collection CONICET Digital (CONICET)
instname_str Consejo Nacional de Investigaciones Científicas y Técnicas
repository.name.fl_str_mv CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicas
repository.mail.fl_str_mv dasensio@conicet.gov.ar; lcarlino@conicet.gov.ar
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score 13.13397

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