The influence of desiccation, cryopreservation and rehydration on the survival of polyembryonic Citrus seeds

Autores
Graiver, Natalia Gisel; Califano, Alicia Noemi; Zaritzky, Noemi Elisabet
Año de publicación
2012
Idioma
inglés
Tipo de recurso
artículo
Estado
versión publicada
Descripción
Three categories of seed storage behavior are generally recognized among plant species: orthodox, intermediate and recalcitrant. Intermediate seeds, such as Citrus, can withstand partial dehydration, but they cannot be stored under conventional genebank conditions because they are cold-sensitive and desiccation does not increase their longevity. Citrus seeds that are immersed in liquid nitrogen (LN) without a previous dehydration process show a very low viability; therefore the water content when they are immersed in LN is considered as the most critical factor in cryopreservation process. The purpose of this study was to investigate the basis of the optimal hydration status for cryopreservation of intermediate oily Citrus seeds: Citrus sinensis (sweet orange) and Citrus paradise (grapefruit). In the present work two conditions were tested: a) seed desiccation, b) seed desiccation followed by LN treatment with thawing under controlled conditions. In addition two pre-sowing (preheating and prehumidification procedures previous to the germinability test) were applied and their influences on the viability of the seeds were analyzed. In the cases of seeds that were only dried and in seeds that were dried and treated with LN, the tested pre-sowing treatments (pre-humidification or heating) did not significantly improve the viability of the seeds with respect to the control samples (P <0.05); in fact preheating deteriorated significantly the viability of the LN treated seeds. The survival of Citrus sinenis and Citrus paradise seeds, submitted to cryopreservation in LN was examined and seed desiccation sensitivity following rehydration procedures was quantified. Results showed that in order to reach the maximum viability, the seeds exposed to LN , must be first dehydrated to a range of ERH 69-81% (0.16-0.31 gH2O g -1dry basis) for Citrus sinensis and 69-75% (0.09-0.11 gH2Og -1dry basis) for Citrus paradise. The limit of hydration for LN Citrus seeds treatment corresponded to the unfrozen water content in the tissue, confirming that seed moisture should be reduced to such an extent to avoid the formation of intra-cellular ice crystals during ultra-rapid freezing, thus preserving the integrity of seed tissues.
Fil: Graiver, Natalia Gisel. Provincia de Buenos Aires. Gobernación. Comisión de Investigaciones Científicas. Centro de Investigación y Desarrollo en Criotecnología de Alimentos. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - La Plata. Centro de Investigación y Desarrollo en Criotecnología de Alimentos. Universidad Nacional de la Plata. Facultad de Ciencias Exactas. Centro de Investigación y Desarrollo en Criotecnología de Alimentos; Argentina
Fil: Califano, Alicia Noemi. Provincia de Buenos Aires. Gobernación. Comisión de Investigaciones Científicas. Centro de Investigación y Desarrollo en Criotecnología de Alimentos. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - La Plata. Centro de Investigación y Desarrollo en Criotecnología de Alimentos. Universidad Nacional de la Plata. Facultad de Ciencias Exactas. Centro de Investigación y Desarrollo en Criotecnología de Alimentos; Argentina
Fil: Zaritzky, Noemi Elisabet. Provincia de Buenos Aires. Gobernación. Comisión de Investigaciones Científicas. Centro de Investigación y Desarrollo en Criotecnología de Alimentos. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - La Plata. Centro de Investigación y Desarrollo en Criotecnología de Alimentos. Universidad Nacional de la Plata. Facultad de Ciencias Exactas. Centro de Investigación y Desarrollo en Criotecnología de Alimentos; Argentina. Universidad Nacional de La Plata. Facultad de Ingenierí­a. Departamento de Ingeniería Química; Argentina
Materia
Citrus Seed
Desiccation
Cryopreservation
Rehydration
Nivel de accesibilidad
acceso abierto
Condiciones de uso
https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
Repositorio
CONICET Digital (CONICET)
Institución
Consejo Nacional de Investigaciones Científicas y Técnicas
OAI Identificador
oai:ri.conicet.gov.ar:11336/72414
Acceder
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oai_identifier_str oai:ri.conicet.gov.ar:11336/72414
network_acronym_str CONICETDig
repository_id_str 3498
network_name_str CONICET Digital (CONICET)
spelling The influence of desiccation, cryopreservation and rehydration on the survival of polyembryonic Citrus seedsGraiver, Natalia GiselCalifano, Alicia NoemiZaritzky, Noemi ElisabetCitrus SeedDesiccationCryopreservationRehydrationhttps://purl.org/becyt/ford/1.4https://purl.org/becyt/ford/1Three categories of seed storage behavior are generally recognized among plant species: orthodox, intermediate and recalcitrant. Intermediate seeds, such as Citrus, can withstand partial dehydration, but they cannot be stored under conventional genebank conditions because they are cold-sensitive and desiccation does not increase their longevity. Citrus seeds that are immersed in liquid nitrogen (LN) without a previous dehydration process show a very low viability; therefore the water content when they are immersed in LN is considered as the most critical factor in cryopreservation process. The purpose of this study was to investigate the basis of the optimal hydration status for cryopreservation of intermediate oily Citrus seeds: Citrus sinensis (sweet orange) and Citrus paradise (grapefruit). In the present work two conditions were tested: a) seed desiccation, b) seed desiccation followed by LN treatment with thawing under controlled conditions. In addition two pre-sowing (preheating and prehumidification procedures previous to the germinability test) were applied and their influences on the viability of the seeds were analyzed. In the cases of seeds that were only dried and in seeds that were dried and treated with LN, the tested pre-sowing treatments (pre-humidification or heating) did not significantly improve the viability of the seeds with respect to the control samples (P <0.05); in fact preheating deteriorated significantly the viability of the LN treated seeds. The survival of Citrus sinenis and Citrus paradise seeds, submitted to cryopreservation in LN was examined and seed desiccation sensitivity following rehydration procedures was quantified. Results showed that in order to reach the maximum viability, the seeds exposed to LN , must be first dehydrated to a range of ERH 69-81% (0.16-0.31 gH2O g -1dry basis) for Citrus sinensis and 69-75% (0.09-0.11 gH2Og -1dry basis) for Citrus paradise. The limit of hydration for LN Citrus seeds treatment corresponded to the unfrozen water content in the tissue, confirming that seed moisture should be reduced to such an extent to avoid the formation of intra-cellular ice crystals during ultra-rapid freezing, thus preserving the integrity of seed tissues.Fil: Graiver, Natalia Gisel. Provincia de Buenos Aires. Gobernación. Comisión de Investigaciones Científicas. Centro de Investigación y Desarrollo en Criotecnología de Alimentos. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - La Plata. Centro de Investigación y Desarrollo en Criotecnología de Alimentos. Universidad Nacional de la Plata. Facultad de Ciencias Exactas. Centro de Investigación y Desarrollo en Criotecnología de Alimentos; ArgentinaFil: Califano, Alicia Noemi. Provincia de Buenos Aires. Gobernación. Comisión de Investigaciones Científicas. Centro de Investigación y Desarrollo en Criotecnología de Alimentos. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - La Plata. Centro de Investigación y Desarrollo en Criotecnología de Alimentos. Universidad Nacional de la Plata. Facultad de Ciencias Exactas. Centro de Investigación y Desarrollo en Criotecnología de Alimentos; ArgentinaFil: Zaritzky, Noemi Elisabet. Provincia de Buenos Aires. Gobernación. Comisión de Investigaciones Científicas. Centro de Investigación y Desarrollo en Criotecnología de Alimentos. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - La Plata. Centro de Investigación y Desarrollo en Criotecnología de Alimentos. Universidad Nacional de la Plata. Facultad de Ciencias Exactas. Centro de Investigación y Desarrollo en Criotecnología de Alimentos; Argentina. Universidad Nacional de La Plata. Facultad de Ingenierí­a. Departamento de Ingeniería Química; ArgentinaAcademic Press Inc Elsevier Science2012-12info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionhttp://purl.org/coar/resource_type/c_6501info:ar-repo/semantics/articuloapplication/pdfapplication/pdfhttp://hdl.handle.net/11336/72414Graiver, Natalia Gisel; Califano, Alicia Noemi; Zaritzky, Noemi Elisabet; The influence of desiccation, cryopreservation and rehydration on the survival of polyembryonic Citrus seeds; Academic Press Inc Elsevier Science; Cryobiology; 65; 3; 12-2012; 346-3470011-2240CONICET DigitalCONICETenginfo:eu-repo/semantics/altIdentifier/url/https://www.sciencedirect.com/science/article/pii/S0011224012001502info:eu-repo/semantics/altIdentifier/doi/10.1016/j.cryobiol.2012.07.026info:eu-repo/semantics/openAccesshttps://creativecommons.org/licenses/by-nc-sa/2.5/ar/reponame:CONICET Digital (CONICET)instname:Consejo Nacional de Investigaciones Científicas y Técnicas2025-09-10T13:20:02Zoai:ri.conicet.gov.ar:11336/72414instacron:CONICETInstitucionalhttp://ri.conicet.gov.ar/Organismo científico-tecnológicoNo correspondehttp://ri.conicet.gov.ar/oai/requestdasensio@conicet.gov.ar; lcarlino@conicet.gov.arArgentinaNo correspondeNo correspondeNo correspondeopendoar:34982025-09-10 13:20:02.701CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicasfalse
dc.title.none.fl_str_mv The influence of desiccation, cryopreservation and rehydration on the survival of polyembryonic Citrus seeds
title The influence of desiccation, cryopreservation and rehydration on the survival of polyembryonic Citrus seeds
spellingShingle The influence of desiccation, cryopreservation and rehydration on the survival of polyembryonic Citrus seeds
Graiver, Natalia Gisel
Citrus Seed
Desiccation
Cryopreservation
Rehydration
title_short The influence of desiccation, cryopreservation and rehydration on the survival of polyembryonic Citrus seeds
title_full The influence of desiccation, cryopreservation and rehydration on the survival of polyembryonic Citrus seeds
title_fullStr The influence of desiccation, cryopreservation and rehydration on the survival of polyembryonic Citrus seeds
title_full_unstemmed The influence of desiccation, cryopreservation and rehydration on the survival of polyembryonic Citrus seeds
title_sort The influence of desiccation, cryopreservation and rehydration on the survival of polyembryonic Citrus seeds
dc.creator.none.fl_str_mv Graiver, Natalia Gisel
Califano, Alicia Noemi
Zaritzky, Noemi Elisabet
author Graiver, Natalia Gisel
author_facet Graiver, Natalia Gisel
Califano, Alicia Noemi
Zaritzky, Noemi Elisabet
author_role author
author2 Califano, Alicia Noemi
Zaritzky, Noemi Elisabet
author2_role author
author
dc.subject.none.fl_str_mv Citrus Seed
Desiccation
Cryopreservation
Rehydration
topic Citrus Seed
Desiccation
Cryopreservation
Rehydration
purl_subject.fl_str_mv https://purl.org/becyt/ford/1.4
https://purl.org/becyt/ford/1
dc.description.none.fl_txt_mv Three categories of seed storage behavior are generally recognized among plant species: orthodox, intermediate and recalcitrant. Intermediate seeds, such as Citrus, can withstand partial dehydration, but they cannot be stored under conventional genebank conditions because they are cold-sensitive and desiccation does not increase their longevity. Citrus seeds that are immersed in liquid nitrogen (LN) without a previous dehydration process show a very low viability; therefore the water content when they are immersed in LN is considered as the most critical factor in cryopreservation process. The purpose of this study was to investigate the basis of the optimal hydration status for cryopreservation of intermediate oily Citrus seeds: Citrus sinensis (sweet orange) and Citrus paradise (grapefruit). In the present work two conditions were tested: a) seed desiccation, b) seed desiccation followed by LN treatment with thawing under controlled conditions. In addition two pre-sowing (preheating and prehumidification procedures previous to the germinability test) were applied and their influences on the viability of the seeds were analyzed. In the cases of seeds that were only dried and in seeds that were dried and treated with LN, the tested pre-sowing treatments (pre-humidification or heating) did not significantly improve the viability of the seeds with respect to the control samples (P <0.05); in fact preheating deteriorated significantly the viability of the LN treated seeds. The survival of Citrus sinenis and Citrus paradise seeds, submitted to cryopreservation in LN was examined and seed desiccation sensitivity following rehydration procedures was quantified. Results showed that in order to reach the maximum viability, the seeds exposed to LN , must be first dehydrated to a range of ERH 69-81% (0.16-0.31 gH2O g -1dry basis) for Citrus sinensis and 69-75% (0.09-0.11 gH2Og -1dry basis) for Citrus paradise. The limit of hydration for LN Citrus seeds treatment corresponded to the unfrozen water content in the tissue, confirming that seed moisture should be reduced to such an extent to avoid the formation of intra-cellular ice crystals during ultra-rapid freezing, thus preserving the integrity of seed tissues.
Fil: Graiver, Natalia Gisel. Provincia de Buenos Aires. Gobernación. Comisión de Investigaciones Científicas. Centro de Investigación y Desarrollo en Criotecnología de Alimentos. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - La Plata. Centro de Investigación y Desarrollo en Criotecnología de Alimentos. Universidad Nacional de la Plata. Facultad de Ciencias Exactas. Centro de Investigación y Desarrollo en Criotecnología de Alimentos; Argentina
Fil: Califano, Alicia Noemi. Provincia de Buenos Aires. Gobernación. Comisión de Investigaciones Científicas. Centro de Investigación y Desarrollo en Criotecnología de Alimentos. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - La Plata. Centro de Investigación y Desarrollo en Criotecnología de Alimentos. Universidad Nacional de la Plata. Facultad de Ciencias Exactas. Centro de Investigación y Desarrollo en Criotecnología de Alimentos; Argentina
Fil: Zaritzky, Noemi Elisabet. Provincia de Buenos Aires. Gobernación. Comisión de Investigaciones Científicas. Centro de Investigación y Desarrollo en Criotecnología de Alimentos. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - La Plata. Centro de Investigación y Desarrollo en Criotecnología de Alimentos. Universidad Nacional de la Plata. Facultad de Ciencias Exactas. Centro de Investigación y Desarrollo en Criotecnología de Alimentos; Argentina. Universidad Nacional de La Plata. Facultad de Ingenierí­a. Departamento de Ingeniería Química; Argentina
description Three categories of seed storage behavior are generally recognized among plant species: orthodox, intermediate and recalcitrant. Intermediate seeds, such as Citrus, can withstand partial dehydration, but they cannot be stored under conventional genebank conditions because they are cold-sensitive and desiccation does not increase their longevity. Citrus seeds that are immersed in liquid nitrogen (LN) without a previous dehydration process show a very low viability; therefore the water content when they are immersed in LN is considered as the most critical factor in cryopreservation process. The purpose of this study was to investigate the basis of the optimal hydration status for cryopreservation of intermediate oily Citrus seeds: Citrus sinensis (sweet orange) and Citrus paradise (grapefruit). In the present work two conditions were tested: a) seed desiccation, b) seed desiccation followed by LN treatment with thawing under controlled conditions. In addition two pre-sowing (preheating and prehumidification procedures previous to the germinability test) were applied and their influences on the viability of the seeds were analyzed. In the cases of seeds that were only dried and in seeds that were dried and treated with LN, the tested pre-sowing treatments (pre-humidification or heating) did not significantly improve the viability of the seeds with respect to the control samples (P <0.05); in fact preheating deteriorated significantly the viability of the LN treated seeds. The survival of Citrus sinenis and Citrus paradise seeds, submitted to cryopreservation in LN was examined and seed desiccation sensitivity following rehydration procedures was quantified. Results showed that in order to reach the maximum viability, the seeds exposed to LN , must be first dehydrated to a range of ERH 69-81% (0.16-0.31 gH2O g -1dry basis) for Citrus sinensis and 69-75% (0.09-0.11 gH2Og -1dry basis) for Citrus paradise. The limit of hydration for LN Citrus seeds treatment corresponded to the unfrozen water content in the tissue, confirming that seed moisture should be reduced to such an extent to avoid the formation of intra-cellular ice crystals during ultra-rapid freezing, thus preserving the integrity of seed tissues.
publishDate 2012
dc.date.none.fl_str_mv 2012-12
dc.type.none.fl_str_mv info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
http://purl.org/coar/resource_type/c_6501
info:ar-repo/semantics/articulo
format article
status_str publishedVersion
dc.identifier.none.fl_str_mv http://hdl.handle.net/11336/72414
Graiver, Natalia Gisel; Califano, Alicia Noemi; Zaritzky, Noemi Elisabet; The influence of desiccation, cryopreservation and rehydration on the survival of polyembryonic Citrus seeds; Academic Press Inc Elsevier Science; Cryobiology; 65; 3; 12-2012; 346-347
0011-2240
CONICET Digital
CONICET
url http://hdl.handle.net/11336/72414
identifier_str_mv Graiver, Natalia Gisel; Califano, Alicia Noemi; Zaritzky, Noemi Elisabet; The influence of desiccation, cryopreservation and rehydration on the survival of polyembryonic Citrus seeds; Academic Press Inc Elsevier Science; Cryobiology; 65; 3; 12-2012; 346-347
0011-2240
CONICET Digital
CONICET
dc.language.none.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv info:eu-repo/semantics/altIdentifier/url/https://www.sciencedirect.com/science/article/pii/S0011224012001502
info:eu-repo/semantics/altIdentifier/doi/10.1016/j.cryobiol.2012.07.026
dc.rights.none.fl_str_mv info:eu-repo/semantics/openAccess
https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
eu_rights_str_mv openAccess
rights_invalid_str_mv https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
dc.format.none.fl_str_mv application/pdf
application/pdf
dc.publisher.none.fl_str_mv Academic Press Inc Elsevier Science
publisher.none.fl_str_mv Academic Press Inc Elsevier Science
dc.source.none.fl_str_mv reponame:CONICET Digital (CONICET)
instname:Consejo Nacional de Investigaciones Científicas y Técnicas
reponame_str CONICET Digital (CONICET)
collection CONICET Digital (CONICET)
instname_str Consejo Nacional de Investigaciones Científicas y Técnicas
repository.name.fl_str_mv CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicas
repository.mail.fl_str_mv dasensio@conicet.gov.ar; lcarlino@conicet.gov.ar
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score 12.48226

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