Magnetic Nanoparticles of La0.78Sr0.22MnO3, Coated with SiO2: Preparation and Cytotoxicity in Human Cell Cultures

Autores
Lassa, Maria Silvina; Gamarra Luques, Carlos Diego; Albornoz, Cecilia Andrea; Leyva de Guglielmino, Ana Gabriela; Vargas Roig, Laura Maria; Vazquez, Patricia Graciela
Año de publicación
2015
Idioma
inglés
Tipo de recurso
artículo
Estado
versión publicada
Descripción
Nanomedicine is a great interesting area for use of magnetic nanoparticles. Among their main applications we can highlight, for example, its use as contrast agents for magnetic resonance imaging, or intracellular delivery, or cellular hyperthermia, which is an employed method in oncology therapy. This study aimed to determine whether the magnetic nanoparticles with La0.78Sr0.22MnO3 (LSMO), coated with SiO2 are cytotoxic to the cancer cell line MCF-7 (human breast adenocarcinoma). For perovskite LSMO synthesis the liquid-mix method was used and sol-gel method was used for SiO2 coating. Cytotoxicity was determined by cell number and viability assessment as well as clonogenic assay to study chronic effect induced by nanoparticles. Concentrations of 74.88 ± 1.1 LSMO μg/ml were able to reduce 50% of cell number compared with control untreated cells and after 72 h of treatment, cells lead a slight chronic effect. We also observed morphological changes after incubation of particles.
Fil: Lassa, Maria Silvina. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Regional de Investigaciones Científicas y Tecnológicas. Laboratorio de Investigaciones y Servicios Ambientales Mendoza; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Mendoza; Argentina
Fil: Gamarra Luques, Carlos Diego. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Mendoza. Instituto de Medicina y Biología Experimental de Cuyo; Argentina. Universidad Nacional de Cuyo. Facultad de Ciencias Médicas; Argentina
Fil: Albornoz, Cecilia Andrea. Comisión Nacional de Energía Atómica; Argentina
Fil: Leyva de Guglielmino, Ana Gabriela. Comisión Nacional de Energía Atómica; Argentina
Fil: Vargas Roig, Laura Maria. Universidad Nacional de Cuyo. Facultad de Ciencias Médicas; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Mendoza. Instituto de Medicina y Biología Experimental de Cuyo; Argentina
Fil: Vazquez, Patricia Graciela. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - La Plata. Centro de Investigación y Desarrollo en Ciencias Aplicadas "Dr. Jorge J. Ronco". Universidad Nacional de La Plata. Facultad de Ciencias Exactas. Centro de Investigación y Desarrollo en Ciencias Aplicadas; Argentina
Materia
Perovskites
Nanomedicine
Breast Cancer
Nivel de accesibilidad
acceso abierto
Condiciones de uso
https://creativecommons.org/licenses/by-nc-nd/2.5/ar/
Repositorio
CONICET Digital (CONICET)
Institución
Consejo Nacional de Investigaciones Científicas y Técnicas
OAI Identificador
oai:ri.conicet.gov.ar:11336/40271
Acceder
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oai_identifier_str oai:ri.conicet.gov.ar:11336/40271
network_acronym_str CONICETDig
repository_id_str 3498
network_name_str CONICET Digital (CONICET)
spelling Magnetic Nanoparticles of La0.78Sr0.22MnO3, Coated with SiO2: Preparation and Cytotoxicity in Human Cell CulturesLassa, Maria SilvinaGamarra Luques, Carlos DiegoAlbornoz, Cecilia AndreaLeyva de Guglielmino, Ana GabrielaVargas Roig, Laura MariaVazquez, Patricia GracielaPerovskitesNanomedicineBreast Cancerhttps://purl.org/becyt/ford/3.1https://purl.org/becyt/ford/3Nanomedicine is a great interesting area for use of magnetic nanoparticles. Among their main applications we can highlight, for example, its use as contrast agents for magnetic resonance imaging, or intracellular delivery, or cellular hyperthermia, which is an employed method in oncology therapy. This study aimed to determine whether the magnetic nanoparticles with La0.78Sr0.22MnO3 (LSMO), coated with SiO2 are cytotoxic to the cancer cell line MCF-7 (human breast adenocarcinoma). For perovskite LSMO synthesis the liquid-mix method was used and sol-gel method was used for SiO2 coating. Cytotoxicity was determined by cell number and viability assessment as well as clonogenic assay to study chronic effect induced by nanoparticles. Concentrations of 74.88 ± 1.1 LSMO μg/ml were able to reduce 50% of cell number compared with control untreated cells and after 72 h of treatment, cells lead a slight chronic effect. We also observed morphological changes after incubation of particles.Fil: Lassa, Maria Silvina. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Regional de Investigaciones Científicas y Tecnológicas. Laboratorio de Investigaciones y Servicios Ambientales Mendoza; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Mendoza; ArgentinaFil: Gamarra Luques, Carlos Diego. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Mendoza. Instituto de Medicina y Biología Experimental de Cuyo; Argentina. Universidad Nacional de Cuyo. Facultad de Ciencias Médicas; ArgentinaFil: Albornoz, Cecilia Andrea. Comisión Nacional de Energía Atómica; ArgentinaFil: Leyva de Guglielmino, Ana Gabriela. Comisión Nacional de Energía Atómica; ArgentinaFil: Vargas Roig, Laura Maria. Universidad Nacional de Cuyo. Facultad de Ciencias Médicas; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Mendoza. Instituto de Medicina y Biología Experimental de Cuyo; ArgentinaFil: Vazquez, Patricia Graciela. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - La Plata. Centro de Investigación y Desarrollo en Ciencias Aplicadas "Dr. Jorge J. Ronco". Universidad Nacional de La Plata. Facultad de Ciencias Exactas. Centro de Investigación y Desarrollo en Ciencias Aplicadas; ArgentinaElsevier2015-06info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionhttp://purl.org/coar/resource_type/c_6501info:ar-repo/semantics/articuloapplication/pdfapplication/pdfhttp://hdl.handle.net/11336/40271Lassa, Maria Silvina; Gamarra Luques, Carlos Diego; Albornoz, Cecilia Andrea; Leyva de Guglielmino, Ana Gabriela; Vargas Roig, Laura Maria; et al.; Magnetic Nanoparticles of La0.78Sr0.22MnO3, Coated with SiO2: Preparation and Cytotoxicity in Human Cell Cultures; Elsevier; Procedia Materials Science; 8; 6-2015; 358-3652211-8128CONICET DigitalCONICETenginfo:eu-repo/semantics/altIdentifier/url/https://www.sciencedirect.com/science/article/pii/S2211812815000863info:eu-repo/semantics/altIdentifier/doi/10.1016/j.mspro.2015.04.085info:eu-repo/semantics/openAccesshttps://creativecommons.org/licenses/by-nc-nd/2.5/ar/reponame:CONICET Digital (CONICET)instname:Consejo Nacional de Investigaciones Científicas y Técnicas2025-10-15T15:30:57Zoai:ri.conicet.gov.ar:11336/40271instacron:CONICETInstitucionalhttp://ri.conicet.gov.ar/Organismo científico-tecnológicoNo correspondehttp://ri.conicet.gov.ar/oai/requestdasensio@conicet.gov.ar; lcarlino@conicet.gov.arArgentinaNo correspondeNo correspondeNo correspondeopendoar:34982025-10-15 15:30:58.147CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicasfalse
dc.title.none.fl_str_mv Magnetic Nanoparticles of La0.78Sr0.22MnO3, Coated with SiO2: Preparation and Cytotoxicity in Human Cell Cultures
title Magnetic Nanoparticles of La0.78Sr0.22MnO3, Coated with SiO2: Preparation and Cytotoxicity in Human Cell Cultures
spellingShingle Magnetic Nanoparticles of La0.78Sr0.22MnO3, Coated with SiO2: Preparation and Cytotoxicity in Human Cell Cultures
Lassa, Maria Silvina
Perovskites
Nanomedicine
Breast Cancer
title_short Magnetic Nanoparticles of La0.78Sr0.22MnO3, Coated with SiO2: Preparation and Cytotoxicity in Human Cell Cultures
title_full Magnetic Nanoparticles of La0.78Sr0.22MnO3, Coated with SiO2: Preparation and Cytotoxicity in Human Cell Cultures
title_fullStr Magnetic Nanoparticles of La0.78Sr0.22MnO3, Coated with SiO2: Preparation and Cytotoxicity in Human Cell Cultures
title_full_unstemmed Magnetic Nanoparticles of La0.78Sr0.22MnO3, Coated with SiO2: Preparation and Cytotoxicity in Human Cell Cultures
title_sort Magnetic Nanoparticles of La0.78Sr0.22MnO3, Coated with SiO2: Preparation and Cytotoxicity in Human Cell Cultures
dc.creator.none.fl_str_mv Lassa, Maria Silvina
Gamarra Luques, Carlos Diego
Albornoz, Cecilia Andrea
Leyva de Guglielmino, Ana Gabriela
Vargas Roig, Laura Maria
Vazquez, Patricia Graciela
author Lassa, Maria Silvina
author_facet Lassa, Maria Silvina
Gamarra Luques, Carlos Diego
Albornoz, Cecilia Andrea
Leyva de Guglielmino, Ana Gabriela
Vargas Roig, Laura Maria
Vazquez, Patricia Graciela
author_role author
author2 Gamarra Luques, Carlos Diego
Albornoz, Cecilia Andrea
Leyva de Guglielmino, Ana Gabriela
Vargas Roig, Laura Maria
Vazquez, Patricia Graciela
author2_role author
author
author
author
author
dc.subject.none.fl_str_mv Perovskites
Nanomedicine
Breast Cancer
topic Perovskites
Nanomedicine
Breast Cancer
purl_subject.fl_str_mv https://purl.org/becyt/ford/3.1
https://purl.org/becyt/ford/3
dc.description.none.fl_txt_mv Nanomedicine is a great interesting area for use of magnetic nanoparticles. Among their main applications we can highlight, for example, its use as contrast agents for magnetic resonance imaging, or intracellular delivery, or cellular hyperthermia, which is an employed method in oncology therapy. This study aimed to determine whether the magnetic nanoparticles with La0.78Sr0.22MnO3 (LSMO), coated with SiO2 are cytotoxic to the cancer cell line MCF-7 (human breast adenocarcinoma). For perovskite LSMO synthesis the liquid-mix method was used and sol-gel method was used for SiO2 coating. Cytotoxicity was determined by cell number and viability assessment as well as clonogenic assay to study chronic effect induced by nanoparticles. Concentrations of 74.88 ± 1.1 LSMO μg/ml were able to reduce 50% of cell number compared with control untreated cells and after 72 h of treatment, cells lead a slight chronic effect. We also observed morphological changes after incubation of particles.
Fil: Lassa, Maria Silvina. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Regional de Investigaciones Científicas y Tecnológicas. Laboratorio de Investigaciones y Servicios Ambientales Mendoza; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Mendoza; Argentina
Fil: Gamarra Luques, Carlos Diego. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Mendoza. Instituto de Medicina y Biología Experimental de Cuyo; Argentina. Universidad Nacional de Cuyo. Facultad de Ciencias Médicas; Argentina
Fil: Albornoz, Cecilia Andrea. Comisión Nacional de Energía Atómica; Argentina
Fil: Leyva de Guglielmino, Ana Gabriela. Comisión Nacional de Energía Atómica; Argentina
Fil: Vargas Roig, Laura Maria. Universidad Nacional de Cuyo. Facultad de Ciencias Médicas; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Mendoza. Instituto de Medicina y Biología Experimental de Cuyo; Argentina
Fil: Vazquez, Patricia Graciela. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - La Plata. Centro de Investigación y Desarrollo en Ciencias Aplicadas "Dr. Jorge J. Ronco". Universidad Nacional de La Plata. Facultad de Ciencias Exactas. Centro de Investigación y Desarrollo en Ciencias Aplicadas; Argentina
description Nanomedicine is a great interesting area for use of magnetic nanoparticles. Among their main applications we can highlight, for example, its use as contrast agents for magnetic resonance imaging, or intracellular delivery, or cellular hyperthermia, which is an employed method in oncology therapy. This study aimed to determine whether the magnetic nanoparticles with La0.78Sr0.22MnO3 (LSMO), coated with SiO2 are cytotoxic to the cancer cell line MCF-7 (human breast adenocarcinoma). For perovskite LSMO synthesis the liquid-mix method was used and sol-gel method was used for SiO2 coating. Cytotoxicity was determined by cell number and viability assessment as well as clonogenic assay to study chronic effect induced by nanoparticles. Concentrations of 74.88 ± 1.1 LSMO μg/ml were able to reduce 50% of cell number compared with control untreated cells and after 72 h of treatment, cells lead a slight chronic effect. We also observed morphological changes after incubation of particles.
publishDate 2015
dc.date.none.fl_str_mv 2015-06
dc.type.none.fl_str_mv info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
http://purl.org/coar/resource_type/c_6501
info:ar-repo/semantics/articulo
format article
status_str publishedVersion
dc.identifier.none.fl_str_mv http://hdl.handle.net/11336/40271
Lassa, Maria Silvina; Gamarra Luques, Carlos Diego; Albornoz, Cecilia Andrea; Leyva de Guglielmino, Ana Gabriela; Vargas Roig, Laura Maria; et al.; Magnetic Nanoparticles of La0.78Sr0.22MnO3, Coated with SiO2: Preparation and Cytotoxicity in Human Cell Cultures; Elsevier; Procedia Materials Science; 8; 6-2015; 358-365
2211-8128
CONICET Digital
CONICET
url http://hdl.handle.net/11336/40271
identifier_str_mv Lassa, Maria Silvina; Gamarra Luques, Carlos Diego; Albornoz, Cecilia Andrea; Leyva de Guglielmino, Ana Gabriela; Vargas Roig, Laura Maria; et al.; Magnetic Nanoparticles of La0.78Sr0.22MnO3, Coated with SiO2: Preparation and Cytotoxicity in Human Cell Cultures; Elsevier; Procedia Materials Science; 8; 6-2015; 358-365
2211-8128
CONICET Digital
CONICET
dc.language.none.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv info:eu-repo/semantics/altIdentifier/url/https://www.sciencedirect.com/science/article/pii/S2211812815000863
info:eu-repo/semantics/altIdentifier/doi/10.1016/j.mspro.2015.04.085
dc.rights.none.fl_str_mv info:eu-repo/semantics/openAccess
https://creativecommons.org/licenses/by-nc-nd/2.5/ar/
eu_rights_str_mv openAccess
rights_invalid_str_mv https://creativecommons.org/licenses/by-nc-nd/2.5/ar/
dc.format.none.fl_str_mv application/pdf
application/pdf
dc.publisher.none.fl_str_mv Elsevier
publisher.none.fl_str_mv Elsevier
dc.source.none.fl_str_mv reponame:CONICET Digital (CONICET)
instname:Consejo Nacional de Investigaciones Científicas y Técnicas
reponame_str CONICET Digital (CONICET)
collection CONICET Digital (CONICET)
instname_str Consejo Nacional de Investigaciones Científicas y Técnicas
repository.name.fl_str_mv CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicas
repository.mail.fl_str_mv dasensio@conicet.gov.ar; lcarlino@conicet.gov.ar
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score 13.22299

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