Cryptic speciation in the Triatoma sordida subcomplex (Hemiptera, Reduviidae) revealed by chromosomal markers

Autores
Panzera, Francisco; Pita, Sebastián; Nattero, Julieta; Panzera, Yanina; Galvão, Cleber; Chavez, Tamara; Rojas De Arias, Antonieta; Cardozo Téllez, Lourdes; Noireau, François
Año de publicación
2015
Idioma
inglés
Tipo de recurso
artículo
Estado
versión publicada
Descripción
Background: Chagas disease vectors (Hemiptera-Reduviidae) comprise more than 140 blood-sucking insect species of the Triatominae subfamily. The largest genus is Triatoma, subdivided in several complexes and subcomplexes according to morphology, ecology and genetic features. One of them is the sordida subcomplex, involving four species: Triatoma sordida, T. guasayana, T. garciabesi and T. patagonica. Given the great morphological similarity of these species, their taxonomic identification, evolutionary relationships and population differentiation have been controversial for many years and even today remain under discussion. Methods: We simultaneously analyzed two chromosomal markers, C-heterochromatin distribution and 45S ribosomal genes chromosomal position, of 139 specimens from several sordida subcomplex populations from Argentina, Bolivia, Brazil and Paraguay, collected both in nature and from several established insectaries. Our results were compared with COI sequences deposited in GenBank. Results: We recognized five chromosomal taxa with putative hybrids, which each differ in at least one chromosome marker. Most of them present significant differences in their mtDNA sequences. Conclusion: The chromosomal taxa here show a significant chromosome differentiation involving changes in the C-heterochromatin content and in the ribosomal clusters position. This paper identifies several erroneously classified populations by morphological methods, delimits the geographical distribution of each taxon and proposes the existence of a new cryptic species, widely distributed in Argentina. We also suggest that sordida sibling species involve closely related as well as evolutionary distant species. Taxonomic status of each chromosomal taxon is discussed considering phenotypic and genetic results previously published.
Fil: Panzera, Francisco. Universidad de la República; Uruguay
Fil: Pita, Sebastián. Universidad de la República; Uruguay
Fil: Nattero, Julieta. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Córdoba. Instituto de Investigaciones Biológicas y Tecnológicas. Universidad Nacional de Córdoba. Facultad de Ciencias Exactas, Físicas y Naturales. Instituto de Investigaciones Biológicas y Tecnológicas; Argentina
Fil: Panzera, Yanina. Universidad de la República; Uruguay
Fil: Galvão, Cleber. Instituto Oswaldo Cruz; Brasil
Fil: Chavez, Tamara. Instituto Nacional de Laboratorios de Salud; Bolivia
Fil: Rojas De Arias, Antonieta. Centro para el Desarrollo de la Investigación Científica; Paraguay
Fil: Cardozo Téllez, Lourdes. Instituto Paraguayo de Tecnología Agraria; Paraguay
Fil: Noireau, François. Centre National de la Recherche Scientifique. Institut de Recherche pour le Développement; Francia
Materia
CHAGAS DISEASE VECTORS
CRYPTIC SPECIES
FISH
HOLOCENTRIC CHROMOSOMES
SORDIDA SUBCOMPLEX SPECIES
TRIATOMINAE
Nivel de accesibilidad
acceso abierto
Condiciones de uso
https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
Repositorio
CONICET Digital (CONICET)
Institución
Consejo Nacional de Investigaciones Científicas y Técnicas
OAI Identificador
oai:ri.conicet.gov.ar:11336/85274
Acceder
id CONICETDig_1956b48609f51c4e920ea014432ce227
oai_identifier_str oai:ri.conicet.gov.ar:11336/85274
network_acronym_str CONICETDig
repository_id_str 3498
network_name_str CONICET Digital (CONICET)
spelling Cryptic speciation in the Triatoma sordida subcomplex (Hemiptera, Reduviidae) revealed by chromosomal markersPanzera, FranciscoPita, SebastiánNattero, JulietaPanzera, YaninaGalvão, CleberChavez, TamaraRojas De Arias, AntonietaCardozo Téllez, LourdesNoireau, FrançoisCHAGAS DISEASE VECTORSCRYPTIC SPECIESFISHHOLOCENTRIC CHROMOSOMESSORDIDA SUBCOMPLEX SPECIESTRIATOMINAEhttps://purl.org/becyt/ford/1.6https://purl.org/becyt/ford/1Background: Chagas disease vectors (Hemiptera-Reduviidae) comprise more than 140 blood-sucking insect species of the Triatominae subfamily. The largest genus is Triatoma, subdivided in several complexes and subcomplexes according to morphology, ecology and genetic features. One of them is the sordida subcomplex, involving four species: Triatoma sordida, T. guasayana, T. garciabesi and T. patagonica. Given the great morphological similarity of these species, their taxonomic identification, evolutionary relationships and population differentiation have been controversial for many years and even today remain under discussion. Methods: We simultaneously analyzed two chromosomal markers, C-heterochromatin distribution and 45S ribosomal genes chromosomal position, of 139 specimens from several sordida subcomplex populations from Argentina, Bolivia, Brazil and Paraguay, collected both in nature and from several established insectaries. Our results were compared with COI sequences deposited in GenBank. Results: We recognized five chromosomal taxa with putative hybrids, which each differ in at least one chromosome marker. Most of them present significant differences in their mtDNA sequences. Conclusion: The chromosomal taxa here show a significant chromosome differentiation involving changes in the C-heterochromatin content and in the ribosomal clusters position. This paper identifies several erroneously classified populations by morphological methods, delimits the geographical distribution of each taxon and proposes the existence of a new cryptic species, widely distributed in Argentina. We also suggest that sordida sibling species involve closely related as well as evolutionary distant species. Taxonomic status of each chromosomal taxon is discussed considering phenotypic and genetic results previously published.Fil: Panzera, Francisco. Universidad de la República; UruguayFil: Pita, Sebastián. Universidad de la República; UruguayFil: Nattero, Julieta. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Córdoba. Instituto de Investigaciones Biológicas y Tecnológicas. Universidad Nacional de Córdoba. Facultad de Ciencias Exactas, Físicas y Naturales. Instituto de Investigaciones Biológicas y Tecnológicas; ArgentinaFil: Panzera, Yanina. Universidad de la República; UruguayFil: Galvão, Cleber. Instituto Oswaldo Cruz; BrasilFil: Chavez, Tamara. Instituto Nacional de Laboratorios de Salud; BoliviaFil: Rojas De Arias, Antonieta. Centro para el Desarrollo de la Investigación Científica; ParaguayFil: Cardozo Téllez, Lourdes. Instituto Paraguayo de Tecnología Agraria; ParaguayFil: Noireau, François. Centre National de la Recherche Scientifique. Institut de Recherche pour le Développement; FranciaBioMed Central2015-09info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionhttp://purl.org/coar/resource_type/c_6501info:ar-repo/semantics/articuloapplication/pdfapplication/pdfhttp://hdl.handle.net/11336/85274Panzera, Francisco; Pita, Sebastián; Nattero, Julieta; Panzera, Yanina; Galvão, Cleber; et al.; Cryptic speciation in the Triatoma sordida subcomplex (Hemiptera, Reduviidae) revealed by chromosomal markers; BioMed Central; Parasites and Vectors; 8; 1; 9-2015; 1-101756-3305CONICET DigitalCONICETenginfo:eu-repo/semantics/altIdentifier/doi/10.1186/s13071-015-1109-6info:eu-repo/semantics/altIdentifier/url/https://parasitesandvectors.biomedcentral.com/articles/10.1186/s13071-015-1109-6info:eu-repo/semantics/openAccesshttps://creativecommons.org/licenses/by-nc-sa/2.5/ar/reponame:CONICET Digital (CONICET)instname:Consejo Nacional de Investigaciones Científicas y Técnicas2025-09-29T10:37:34Zoai:ri.conicet.gov.ar:11336/85274instacron:CONICETInstitucionalhttp://ri.conicet.gov.ar/Organismo científico-tecnológicoNo correspondehttp://ri.conicet.gov.ar/oai/requestdasensio@conicet.gov.ar; lcarlino@conicet.gov.arArgentinaNo correspondeNo correspondeNo correspondeopendoar:34982025-09-29 10:37:35.023CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicasfalse
dc.title.none.fl_str_mv Cryptic speciation in the Triatoma sordida subcomplex (Hemiptera, Reduviidae) revealed by chromosomal markers
title Cryptic speciation in the Triatoma sordida subcomplex (Hemiptera, Reduviidae) revealed by chromosomal markers
spellingShingle Cryptic speciation in the Triatoma sordida subcomplex (Hemiptera, Reduviidae) revealed by chromosomal markers
Panzera, Francisco
CHAGAS DISEASE VECTORS
CRYPTIC SPECIES
FISH
HOLOCENTRIC CHROMOSOMES
SORDIDA SUBCOMPLEX SPECIES
TRIATOMINAE
title_short Cryptic speciation in the Triatoma sordida subcomplex (Hemiptera, Reduviidae) revealed by chromosomal markers
title_full Cryptic speciation in the Triatoma sordida subcomplex (Hemiptera, Reduviidae) revealed by chromosomal markers
title_fullStr Cryptic speciation in the Triatoma sordida subcomplex (Hemiptera, Reduviidae) revealed by chromosomal markers
title_full_unstemmed Cryptic speciation in the Triatoma sordida subcomplex (Hemiptera, Reduviidae) revealed by chromosomal markers
title_sort Cryptic speciation in the Triatoma sordida subcomplex (Hemiptera, Reduviidae) revealed by chromosomal markers
dc.creator.none.fl_str_mv Panzera, Francisco
Pita, Sebastián
Nattero, Julieta
Panzera, Yanina
Galvão, Cleber
Chavez, Tamara
Rojas De Arias, Antonieta
Cardozo Téllez, Lourdes
Noireau, François
author Panzera, Francisco
author_facet Panzera, Francisco
Pita, Sebastián
Nattero, Julieta
Panzera, Yanina
Galvão, Cleber
Chavez, Tamara
Rojas De Arias, Antonieta
Cardozo Téllez, Lourdes
Noireau, François
author_role author
author2 Pita, Sebastián
Nattero, Julieta
Panzera, Yanina
Galvão, Cleber
Chavez, Tamara
Rojas De Arias, Antonieta
Cardozo Téllez, Lourdes
Noireau, François
author2_role author
author
author
author
author
author
author
author
dc.subject.none.fl_str_mv CHAGAS DISEASE VECTORS
CRYPTIC SPECIES
FISH
HOLOCENTRIC CHROMOSOMES
SORDIDA SUBCOMPLEX SPECIES
TRIATOMINAE
topic CHAGAS DISEASE VECTORS
CRYPTIC SPECIES
FISH
HOLOCENTRIC CHROMOSOMES
SORDIDA SUBCOMPLEX SPECIES
TRIATOMINAE
purl_subject.fl_str_mv https://purl.org/becyt/ford/1.6
https://purl.org/becyt/ford/1
dc.description.none.fl_txt_mv Background: Chagas disease vectors (Hemiptera-Reduviidae) comprise more than 140 blood-sucking insect species of the Triatominae subfamily. The largest genus is Triatoma, subdivided in several complexes and subcomplexes according to morphology, ecology and genetic features. One of them is the sordida subcomplex, involving four species: Triatoma sordida, T. guasayana, T. garciabesi and T. patagonica. Given the great morphological similarity of these species, their taxonomic identification, evolutionary relationships and population differentiation have been controversial for many years and even today remain under discussion. Methods: We simultaneously analyzed two chromosomal markers, C-heterochromatin distribution and 45S ribosomal genes chromosomal position, of 139 specimens from several sordida subcomplex populations from Argentina, Bolivia, Brazil and Paraguay, collected both in nature and from several established insectaries. Our results were compared with COI sequences deposited in GenBank. Results: We recognized five chromosomal taxa with putative hybrids, which each differ in at least one chromosome marker. Most of them present significant differences in their mtDNA sequences. Conclusion: The chromosomal taxa here show a significant chromosome differentiation involving changes in the C-heterochromatin content and in the ribosomal clusters position. This paper identifies several erroneously classified populations by morphological methods, delimits the geographical distribution of each taxon and proposes the existence of a new cryptic species, widely distributed in Argentina. We also suggest that sordida sibling species involve closely related as well as evolutionary distant species. Taxonomic status of each chromosomal taxon is discussed considering phenotypic and genetic results previously published.
Fil: Panzera, Francisco. Universidad de la República; Uruguay
Fil: Pita, Sebastián. Universidad de la República; Uruguay
Fil: Nattero, Julieta. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Córdoba. Instituto de Investigaciones Biológicas y Tecnológicas. Universidad Nacional de Córdoba. Facultad de Ciencias Exactas, Físicas y Naturales. Instituto de Investigaciones Biológicas y Tecnológicas; Argentina
Fil: Panzera, Yanina. Universidad de la República; Uruguay
Fil: Galvão, Cleber. Instituto Oswaldo Cruz; Brasil
Fil: Chavez, Tamara. Instituto Nacional de Laboratorios de Salud; Bolivia
Fil: Rojas De Arias, Antonieta. Centro para el Desarrollo de la Investigación Científica; Paraguay
Fil: Cardozo Téllez, Lourdes. Instituto Paraguayo de Tecnología Agraria; Paraguay
Fil: Noireau, François. Centre National de la Recherche Scientifique. Institut de Recherche pour le Développement; Francia
description Background: Chagas disease vectors (Hemiptera-Reduviidae) comprise more than 140 blood-sucking insect species of the Triatominae subfamily. The largest genus is Triatoma, subdivided in several complexes and subcomplexes according to morphology, ecology and genetic features. One of them is the sordida subcomplex, involving four species: Triatoma sordida, T. guasayana, T. garciabesi and T. patagonica. Given the great morphological similarity of these species, their taxonomic identification, evolutionary relationships and population differentiation have been controversial for many years and even today remain under discussion. Methods: We simultaneously analyzed two chromosomal markers, C-heterochromatin distribution and 45S ribosomal genes chromosomal position, of 139 specimens from several sordida subcomplex populations from Argentina, Bolivia, Brazil and Paraguay, collected both in nature and from several established insectaries. Our results were compared with COI sequences deposited in GenBank. Results: We recognized five chromosomal taxa with putative hybrids, which each differ in at least one chromosome marker. Most of them present significant differences in their mtDNA sequences. Conclusion: The chromosomal taxa here show a significant chromosome differentiation involving changes in the C-heterochromatin content and in the ribosomal clusters position. This paper identifies several erroneously classified populations by morphological methods, delimits the geographical distribution of each taxon and proposes the existence of a new cryptic species, widely distributed in Argentina. We also suggest that sordida sibling species involve closely related as well as evolutionary distant species. Taxonomic status of each chromosomal taxon is discussed considering phenotypic and genetic results previously published.
publishDate 2015
dc.date.none.fl_str_mv 2015-09
dc.type.none.fl_str_mv info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
http://purl.org/coar/resource_type/c_6501
info:ar-repo/semantics/articulo
format article
status_str publishedVersion
dc.identifier.none.fl_str_mv http://hdl.handle.net/11336/85274
Panzera, Francisco; Pita, Sebastián; Nattero, Julieta; Panzera, Yanina; Galvão, Cleber; et al.; Cryptic speciation in the Triatoma sordida subcomplex (Hemiptera, Reduviidae) revealed by chromosomal markers; BioMed Central; Parasites and Vectors; 8; 1; 9-2015; 1-10
1756-3305
CONICET Digital
CONICET
url http://hdl.handle.net/11336/85274
identifier_str_mv Panzera, Francisco; Pita, Sebastián; Nattero, Julieta; Panzera, Yanina; Galvão, Cleber; et al.; Cryptic speciation in the Triatoma sordida subcomplex (Hemiptera, Reduviidae) revealed by chromosomal markers; BioMed Central; Parasites and Vectors; 8; 1; 9-2015; 1-10
1756-3305
CONICET Digital
CONICET
dc.language.none.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv info:eu-repo/semantics/altIdentifier/doi/10.1186/s13071-015-1109-6
info:eu-repo/semantics/altIdentifier/url/https://parasitesandvectors.biomedcentral.com/articles/10.1186/s13071-015-1109-6
dc.rights.none.fl_str_mv info:eu-repo/semantics/openAccess
https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
eu_rights_str_mv openAccess
rights_invalid_str_mv https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
dc.format.none.fl_str_mv application/pdf
application/pdf
dc.publisher.none.fl_str_mv BioMed Central
publisher.none.fl_str_mv BioMed Central
dc.source.none.fl_str_mv reponame:CONICET Digital (CONICET)
instname:Consejo Nacional de Investigaciones Científicas y Técnicas
reponame_str CONICET Digital (CONICET)
collection CONICET Digital (CONICET)
instname_str Consejo Nacional de Investigaciones Científicas y Técnicas
repository.name.fl_str_mv CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicas
repository.mail.fl_str_mv dasensio@conicet.gov.ar; lcarlino@conicet.gov.ar
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score 13.070432

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