Troponin T3 regulates nuclear localization of the calcium channel Cavβ1a subunit in skeletal muscle

Autores
Zhang, Tan; Taylor, Jackson; Jiang, Yang; Pereyra, Andrea Soledad; Messi, Maria Laura; Wang, Zhong Min; Hereñú, Claudia Beatriz; Delbono, Osvaldo
Año de publicación
2015
Idioma
inglés
Tipo de recurso
artículo
Estado
versión publicada
Descripción
The voltage-gated calcium channel (Cav) β1a subunit (Cavβ1a) plays an important role in excitation-contraction coupling (ECC), a process in the myoplasm that leads to muscle-force generation. Recently, we discovered that the Cavβ1a subunit travels to the nucleus of skeletal muscle cells where it helps to regulate gene transcription. To determine how it travels to the nucleus, we performed a yeast two-hybrid screening of the mouse fast skeletal muscle cDNA library and identified an interaction with troponin T3 (TnT3), which we subsequently confirmed by co-immunoprecipitation and co-localization assays in mouse skeletal muscle in vivo and in cultured C2C12 muscle cells. Interacting domains were mapped to the leucine zipper domain in TnT3 COOH-terminus (160-244 aa) and Cavβ1a NH2-terminus (1-99 aa), respectively. The double fluorescence assay in C2C12 cells co-expressing TnT3/DsRed and Cavβ1a/YFP shows that TnT3 facilitates Cavβ1a nuclear recruitment, suggesting that the two proteins play a heretofore unknown role during early muscle differentiation in addition to their classical role in ECC regulation.
Fil: Zhang, Tan. Wake Forest School of Medicine; Estados Unidos
Fil: Taylor, Jackson. Wake Forest School of Medicine; Estados Unidos
Fil: Jiang, Yang. Wake Forest School of Medicine; Estados Unidos
Fil: Pereyra, Andrea Soledad. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - La Plata. Instituto de Investigaciones Bioquímicas de La Plata "Prof. Dr. Rodolfo R. Brenner". Universidad Nacional de la Plata. Facultad de Ciencias Médicas. Instituto de Investigaciones Bioquímicas de La Plata ; Argentina
Fil: Messi, Maria Laura. Wake Forest School of Medicine; Estados Unidos
Fil: Wang, Zhong Min. Wake Forest School of Medicine; Estados Unidos
Fil: Hereñú, Claudia Beatriz. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - La Plata. Instituto de Investigaciones Bioquímicas de La Plata "Prof. Dr. Rodolfo R. Brenner". Universidad Nacional de la Plata. Facultad de Ciencias Médicas. Instituto de Investigaciones Bioquímicas de La Plata ; Argentina
Fil: Delbono, Osvaldo. Wake Forest School of Medicine; Estados Unidos
Materia
Cavβ1a
Nuclear Localization
Skeletal Muscle
Troponin T3
Nivel de accesibilidad
acceso abierto
Condiciones de uso
https://creativecommons.org/licenses/by-nc-nd/2.5/ar/
Repositorio
CONICET Digital (CONICET)
Institución
Consejo Nacional de Investigaciones Científicas y Técnicas
OAI Identificador
oai:ri.conicet.gov.ar:11336/48902
Acceder
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oai_identifier_str oai:ri.conicet.gov.ar:11336/48902
network_acronym_str CONICETDig
repository_id_str 3498
network_name_str CONICET Digital (CONICET)
spelling Troponin T3 regulates nuclear localization of the calcium channel Cavβ1a subunit in skeletal muscleZhang, TanTaylor, JacksonJiang, YangPereyra, Andrea SoledadMessi, Maria LauraWang, Zhong MinHereñú, Claudia BeatrizDelbono, OsvaldoCavβ1aNuclear LocalizationSkeletal MuscleTroponin T3https://purl.org/becyt/ford/3.1https://purl.org/becyt/ford/3The voltage-gated calcium channel (Cav) β1a subunit (Cavβ1a) plays an important role in excitation-contraction coupling (ECC), a process in the myoplasm that leads to muscle-force generation. Recently, we discovered that the Cavβ1a subunit travels to the nucleus of skeletal muscle cells where it helps to regulate gene transcription. To determine how it travels to the nucleus, we performed a yeast two-hybrid screening of the mouse fast skeletal muscle cDNA library and identified an interaction with troponin T3 (TnT3), which we subsequently confirmed by co-immunoprecipitation and co-localization assays in mouse skeletal muscle in vivo and in cultured C2C12 muscle cells. Interacting domains were mapped to the leucine zipper domain in TnT3 COOH-terminus (160-244 aa) and Cavβ1a NH2-terminus (1-99 aa), respectively. The double fluorescence assay in C2C12 cells co-expressing TnT3/DsRed and Cavβ1a/YFP shows that TnT3 facilitates Cavβ1a nuclear recruitment, suggesting that the two proteins play a heretofore unknown role during early muscle differentiation in addition to their classical role in ECC regulation.Fil: Zhang, Tan. Wake Forest School of Medicine; Estados UnidosFil: Taylor, Jackson. Wake Forest School of Medicine; Estados UnidosFil: Jiang, Yang. Wake Forest School of Medicine; Estados UnidosFil: Pereyra, Andrea Soledad. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - La Plata. Instituto de Investigaciones Bioquímicas de La Plata "Prof. Dr. Rodolfo R. Brenner". Universidad Nacional de la Plata. Facultad de Ciencias Médicas. Instituto de Investigaciones Bioquímicas de La Plata ; ArgentinaFil: Messi, Maria Laura. Wake Forest School of Medicine; Estados UnidosFil: Wang, Zhong Min. Wake Forest School of Medicine; Estados UnidosFil: Hereñú, Claudia Beatriz. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - La Plata. Instituto de Investigaciones Bioquímicas de La Plata "Prof. Dr. Rodolfo R. Brenner". Universidad Nacional de la Plata. Facultad de Ciencias Médicas. Instituto de Investigaciones Bioquímicas de La Plata ; ArgentinaFil: Delbono, Osvaldo. Wake Forest School of Medicine; Estados UnidosElsevier Inc2015-08info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionhttp://purl.org/coar/resource_type/c_6501info:ar-repo/semantics/articuloapplication/pdfapplication/pdfapplication/pdfapplication/pdfhttp://hdl.handle.net/11336/48902Zhang, Tan; Taylor, Jackson; Jiang, Yang; Pereyra, Andrea Soledad; Messi, Maria Laura; et al.; Troponin T3 regulates nuclear localization of the calcium channel Cavβ1a subunit in skeletal muscle; Elsevier Inc; Experimental Cell Research; 336; 2; 8-2015; 276-2860014-4827CONICET DigitalCONICETenginfo:eu-repo/semantics/altIdentifier/doi/10.1016/j.yexcr.2015.05.005info:eu-repo/semantics/altIdentifier/url/https://www.sciencedirect.com/science/article/pii/S0014482715001743info:eu-repo/semantics/altIdentifier/url/https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4522384/info:eu-repo/semantics/openAccesshttps://creativecommons.org/licenses/by-nc-nd/2.5/ar/reponame:CONICET Digital (CONICET)instname:Consejo Nacional de Investigaciones Científicas y Técnicas2025-09-03T09:56:48Zoai:ri.conicet.gov.ar:11336/48902instacron:CONICETInstitucionalhttp://ri.conicet.gov.ar/Organismo científico-tecnológicoNo correspondehttp://ri.conicet.gov.ar/oai/requestdasensio@conicet.gov.ar; lcarlino@conicet.gov.arArgentinaNo correspondeNo correspondeNo correspondeopendoar:34982025-09-03 09:56:48.501CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicasfalse
dc.title.none.fl_str_mv Troponin T3 regulates nuclear localization of the calcium channel Cavβ1a subunit in skeletal muscle
title Troponin T3 regulates nuclear localization of the calcium channel Cavβ1a subunit in skeletal muscle
spellingShingle Troponin T3 regulates nuclear localization of the calcium channel Cavβ1a subunit in skeletal muscle
Zhang, Tan
Cavβ1a
Nuclear Localization
Skeletal Muscle
Troponin T3
title_short Troponin T3 regulates nuclear localization of the calcium channel Cavβ1a subunit in skeletal muscle
title_full Troponin T3 regulates nuclear localization of the calcium channel Cavβ1a subunit in skeletal muscle
title_fullStr Troponin T3 regulates nuclear localization of the calcium channel Cavβ1a subunit in skeletal muscle
title_full_unstemmed Troponin T3 regulates nuclear localization of the calcium channel Cavβ1a subunit in skeletal muscle
title_sort Troponin T3 regulates nuclear localization of the calcium channel Cavβ1a subunit in skeletal muscle
dc.creator.none.fl_str_mv Zhang, Tan
Taylor, Jackson
Jiang, Yang
Pereyra, Andrea Soledad
Messi, Maria Laura
Wang, Zhong Min
Hereñú, Claudia Beatriz
Delbono, Osvaldo
author Zhang, Tan
author_facet Zhang, Tan
Taylor, Jackson
Jiang, Yang
Pereyra, Andrea Soledad
Messi, Maria Laura
Wang, Zhong Min
Hereñú, Claudia Beatriz
Delbono, Osvaldo
author_role author
author2 Taylor, Jackson
Jiang, Yang
Pereyra, Andrea Soledad
Messi, Maria Laura
Wang, Zhong Min
Hereñú, Claudia Beatriz
Delbono, Osvaldo
author2_role author
author
author
author
author
author
author
dc.subject.none.fl_str_mv Cavβ1a
Nuclear Localization
Skeletal Muscle
Troponin T3
topic Cavβ1a
Nuclear Localization
Skeletal Muscle
Troponin T3
purl_subject.fl_str_mv https://purl.org/becyt/ford/3.1
https://purl.org/becyt/ford/3
dc.description.none.fl_txt_mv The voltage-gated calcium channel (Cav) β1a subunit (Cavβ1a) plays an important role in excitation-contraction coupling (ECC), a process in the myoplasm that leads to muscle-force generation. Recently, we discovered that the Cavβ1a subunit travels to the nucleus of skeletal muscle cells where it helps to regulate gene transcription. To determine how it travels to the nucleus, we performed a yeast two-hybrid screening of the mouse fast skeletal muscle cDNA library and identified an interaction with troponin T3 (TnT3), which we subsequently confirmed by co-immunoprecipitation and co-localization assays in mouse skeletal muscle in vivo and in cultured C2C12 muscle cells. Interacting domains were mapped to the leucine zipper domain in TnT3 COOH-terminus (160-244 aa) and Cavβ1a NH2-terminus (1-99 aa), respectively. The double fluorescence assay in C2C12 cells co-expressing TnT3/DsRed and Cavβ1a/YFP shows that TnT3 facilitates Cavβ1a nuclear recruitment, suggesting that the two proteins play a heretofore unknown role during early muscle differentiation in addition to their classical role in ECC regulation.
Fil: Zhang, Tan. Wake Forest School of Medicine; Estados Unidos
Fil: Taylor, Jackson. Wake Forest School of Medicine; Estados Unidos
Fil: Jiang, Yang. Wake Forest School of Medicine; Estados Unidos
Fil: Pereyra, Andrea Soledad. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - La Plata. Instituto de Investigaciones Bioquímicas de La Plata "Prof. Dr. Rodolfo R. Brenner". Universidad Nacional de la Plata. Facultad de Ciencias Médicas. Instituto de Investigaciones Bioquímicas de La Plata ; Argentina
Fil: Messi, Maria Laura. Wake Forest School of Medicine; Estados Unidos
Fil: Wang, Zhong Min. Wake Forest School of Medicine; Estados Unidos
Fil: Hereñú, Claudia Beatriz. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - La Plata. Instituto de Investigaciones Bioquímicas de La Plata "Prof. Dr. Rodolfo R. Brenner". Universidad Nacional de la Plata. Facultad de Ciencias Médicas. Instituto de Investigaciones Bioquímicas de La Plata ; Argentina
Fil: Delbono, Osvaldo. Wake Forest School of Medicine; Estados Unidos
description The voltage-gated calcium channel (Cav) β1a subunit (Cavβ1a) plays an important role in excitation-contraction coupling (ECC), a process in the myoplasm that leads to muscle-force generation. Recently, we discovered that the Cavβ1a subunit travels to the nucleus of skeletal muscle cells where it helps to regulate gene transcription. To determine how it travels to the nucleus, we performed a yeast two-hybrid screening of the mouse fast skeletal muscle cDNA library and identified an interaction with troponin T3 (TnT3), which we subsequently confirmed by co-immunoprecipitation and co-localization assays in mouse skeletal muscle in vivo and in cultured C2C12 muscle cells. Interacting domains were mapped to the leucine zipper domain in TnT3 COOH-terminus (160-244 aa) and Cavβ1a NH2-terminus (1-99 aa), respectively. The double fluorescence assay in C2C12 cells co-expressing TnT3/DsRed and Cavβ1a/YFP shows that TnT3 facilitates Cavβ1a nuclear recruitment, suggesting that the two proteins play a heretofore unknown role during early muscle differentiation in addition to their classical role in ECC regulation.
publishDate 2015
dc.date.none.fl_str_mv 2015-08
dc.type.none.fl_str_mv info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
http://purl.org/coar/resource_type/c_6501
info:ar-repo/semantics/articulo
format article
status_str publishedVersion
dc.identifier.none.fl_str_mv http://hdl.handle.net/11336/48902
Zhang, Tan; Taylor, Jackson; Jiang, Yang; Pereyra, Andrea Soledad; Messi, Maria Laura; et al.; Troponin T3 regulates nuclear localization of the calcium channel Cavβ1a subunit in skeletal muscle; Elsevier Inc; Experimental Cell Research; 336; 2; 8-2015; 276-286
0014-4827
CONICET Digital
CONICET
url http://hdl.handle.net/11336/48902
identifier_str_mv Zhang, Tan; Taylor, Jackson; Jiang, Yang; Pereyra, Andrea Soledad; Messi, Maria Laura; et al.; Troponin T3 regulates nuclear localization of the calcium channel Cavβ1a subunit in skeletal muscle; Elsevier Inc; Experimental Cell Research; 336; 2; 8-2015; 276-286
0014-4827
CONICET Digital
CONICET
dc.language.none.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv info:eu-repo/semantics/altIdentifier/doi/10.1016/j.yexcr.2015.05.005
info:eu-repo/semantics/altIdentifier/url/https://www.sciencedirect.com/science/article/pii/S0014482715001743
info:eu-repo/semantics/altIdentifier/url/https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4522384/
dc.rights.none.fl_str_mv info:eu-repo/semantics/openAccess
https://creativecommons.org/licenses/by-nc-nd/2.5/ar/
eu_rights_str_mv openAccess
rights_invalid_str_mv https://creativecommons.org/licenses/by-nc-nd/2.5/ar/
dc.format.none.fl_str_mv application/pdf
application/pdf
application/pdf
application/pdf
dc.publisher.none.fl_str_mv Elsevier Inc
publisher.none.fl_str_mv Elsevier Inc
dc.source.none.fl_str_mv reponame:CONICET Digital (CONICET)
instname:Consejo Nacional de Investigaciones Científicas y Técnicas
reponame_str CONICET Digital (CONICET)
collection CONICET Digital (CONICET)
instname_str Consejo Nacional de Investigaciones Científicas y Técnicas
repository.name.fl_str_mv CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicas
repository.mail.fl_str_mv dasensio@conicet.gov.ar; lcarlino@conicet.gov.ar
_version_ 1842269424796565504
score 13.13397

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