No Influence of bcl-2, p53, and p21 waf1 Protein Expression on the Outcome of Pediatric Hodgkin Lymphomas

Autores
Chabay, Paola Andrea; Pesce, Pablo Adrian; de Matteo, Elena Noemí; García Lombardi, Mercedes; Rey, Guadalupe; Preciado, María Victoria
Año de publicación
2006
Idioma
inglés
Tipo de recurso
artículo
Estado
versión publicada
Descripción
In Argentina, lymphomas account for 13.6% of all pediatric tumors and 47% of them are Hodgkin lymphoma. Previous studies of lymphoma series have reported the expression of apoptotic and cell cycle proteins. Our aim was to study these markers in our pediatric patients and correlate them with their outcome. Immunohistochemical staining with monoclonal antibodies anti-p53, bcl-2, p21waf1, and mdm2 were performed on formalin fixed paraffin-embedded Hodgkin lymphoma lymph node biopsies from 54 pediatric patients. The analyzed oncogenes p53, bcl-2, p21waf1, and mdm2 exhibited 81%, 44%, 76%, and 90% positive staining, respectively. The most prevalent p53/p21waf1 expression pattern was p53+/p21waf1+, in 57% of cases, whereas concerning p53/mdm2 expression pattern p53+/mdm2+ was observed in 61% of cases. We failed to .nd any statistically signi.cant correlation between oncogene expression and patient’s survival. It seems that p53 plays an important role in lymphomagenesis in our studied population, because it is overexpressed in 81% of Hodgkin lymphoma cases and in more than 50% of cases, it might be able to activate its cellular e.ectors. Bcl-2 staining observed in 44% of our cases could represent a failure in bcl-2 down-regulation that leads to a rescue event in defective germinal center B-cells, that allows them to develop into Reed-Sternberg and Hodgkin cells.
Fil: Chabay, Paola Andrea. Gobierno de la Ciudad de Buenos Aires. Hospital General de Niños "Ricardo Gutiérrez"; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina
Fil: Pesce, Pablo Adrian. No especifíca;
Fil: de Matteo, Elena Noemí. Gobierno de la Ciudad de Buenos Aires. Hospital General de Niños "Ricardo Gutiérrez"; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina
Fil: García Lombardi, Mercedes. No especifíca;
Fil: Rey, Guadalupe. No especifíca;
Fil: Preciado, María Victoria. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina. Gobierno de la Ciudad de Buenos Aires. Hospital General de Niños "Ricardo Gutiérrez"; Argentina
Materia
HODGKIN LYMPHOMA
CHILDREN
OUTCOME
Nivel de accesibilidad
acceso abierto
Condiciones de uso
https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
Repositorio
CONICET Digital (CONICET)
Institución
Consejo Nacional de Investigaciones Científicas y Técnicas
OAI Identificador
oai:ri.conicet.gov.ar:11336/245205
Acceder
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oai_identifier_str oai:ri.conicet.gov.ar:11336/245205
network_acronym_str CONICETDig
repository_id_str 3498
network_name_str CONICET Digital (CONICET)
spelling No Influence of bcl-2, p53, and p21 waf1 Protein Expression on the Outcome of Pediatric Hodgkin LymphomasChabay, Paola AndreaPesce, Pablo Adriande Matteo, Elena NoemíGarcía Lombardi, MercedesRey, GuadalupePreciado, María VictoriaHODGKIN LYMPHOMACHILDRENOUTCOMEhttps://purl.org/becyt/ford/3.5https://purl.org/becyt/ford/3In Argentina, lymphomas account for 13.6% of all pediatric tumors and 47% of them are Hodgkin lymphoma. Previous studies of lymphoma series have reported the expression of apoptotic and cell cycle proteins. Our aim was to study these markers in our pediatric patients and correlate them with their outcome. Immunohistochemical staining with monoclonal antibodies anti-p53, bcl-2, p21waf1, and mdm2 were performed on formalin fixed paraffin-embedded Hodgkin lymphoma lymph node biopsies from 54 pediatric patients. The analyzed oncogenes p53, bcl-2, p21waf1, and mdm2 exhibited 81%, 44%, 76%, and 90% positive staining, respectively. The most prevalent p53/p21waf1 expression pattern was p53+/p21waf1+, in 57% of cases, whereas concerning p53/mdm2 expression pattern p53+/mdm2+ was observed in 61% of cases. We failed to .nd any statistically signi.cant correlation between oncogene expression and patient’s survival. It seems that p53 plays an important role in lymphomagenesis in our studied population, because it is overexpressed in 81% of Hodgkin lymphoma cases and in more than 50% of cases, it might be able to activate its cellular e.ectors. Bcl-2 staining observed in 44% of our cases could represent a failure in bcl-2 down-regulation that leads to a rescue event in defective germinal center B-cells, that allows them to develop into Reed-Sternberg and Hodgkin cells.Fil: Chabay, Paola Andrea. Gobierno de la Ciudad de Buenos Aires. Hospital General de Niños "Ricardo Gutiérrez"; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; ArgentinaFil: Pesce, Pablo Adrian. No especifíca;Fil: de Matteo, Elena Noemí. Gobierno de la Ciudad de Buenos Aires. Hospital General de Niños "Ricardo Gutiérrez"; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; ArgentinaFil: García Lombardi, Mercedes. No especifíca;Fil: Rey, Guadalupe. No especifíca;Fil: Preciado, María Victoria. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina. Gobierno de la Ciudad de Buenos Aires. Hospital General de Niños "Ricardo Gutiérrez"; ArgentinaLippincott Williams2006-09info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionhttp://purl.org/coar/resource_type/c_6501info:ar-repo/semantics/articuloapplication/pdfapplication/pdfapplication/pdfapplication/pdfhttp://hdl.handle.net/11336/245205Chabay, Paola Andrea; Pesce, Pablo Adrian; de Matteo, Elena Noemí; García Lombardi, Mercedes; Rey, Guadalupe; et al.; No Influence of bcl-2, p53, and p21 waf1 Protein Expression on the Outcome of Pediatric Hodgkin Lymphomas; Lippincott Williams; Journal Of Pediatric Hematology Oncology; 28; 9; 9-2006; 552-5581077-4114CONICET DigitalCONICETenginfo:eu-repo/semantics/altIdentifier/url/https://journals.lww.com/jpho-online/abstract/2006/09000/no_influence_of_bcl_2,_p53,_and_p21waf1_protein.2.aspxinfo:eu-repo/semantics/altIdentifier/doi/10.1097/01.mph.0000212955.43350.bbinfo:eu-repo/semantics/openAccesshttps://creativecommons.org/licenses/by-nc-sa/2.5/ar/reponame:CONICET Digital (CONICET)instname:Consejo Nacional de Investigaciones Científicas y Técnicas2025-09-29T10:18:06Zoai:ri.conicet.gov.ar:11336/245205instacron:CONICETInstitucionalhttp://ri.conicet.gov.ar/Organismo científico-tecnológicoNo correspondehttp://ri.conicet.gov.ar/oai/requestdasensio@conicet.gov.ar; lcarlino@conicet.gov.arArgentinaNo correspondeNo correspondeNo correspondeopendoar:34982025-09-29 10:18:07.183CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicasfalse
dc.title.none.fl_str_mv No Influence of bcl-2, p53, and p21 waf1 Protein Expression on the Outcome of Pediatric Hodgkin Lymphomas
title No Influence of bcl-2, p53, and p21 waf1 Protein Expression on the Outcome of Pediatric Hodgkin Lymphomas
spellingShingle No Influence of bcl-2, p53, and p21 waf1 Protein Expression on the Outcome of Pediatric Hodgkin Lymphomas
Chabay, Paola Andrea
HODGKIN LYMPHOMA
CHILDREN
OUTCOME
title_short No Influence of bcl-2, p53, and p21 waf1 Protein Expression on the Outcome of Pediatric Hodgkin Lymphomas
title_full No Influence of bcl-2, p53, and p21 waf1 Protein Expression on the Outcome of Pediatric Hodgkin Lymphomas
title_fullStr No Influence of bcl-2, p53, and p21 waf1 Protein Expression on the Outcome of Pediatric Hodgkin Lymphomas
title_full_unstemmed No Influence of bcl-2, p53, and p21 waf1 Protein Expression on the Outcome of Pediatric Hodgkin Lymphomas
title_sort No Influence of bcl-2, p53, and p21 waf1 Protein Expression on the Outcome of Pediatric Hodgkin Lymphomas
dc.creator.none.fl_str_mv Chabay, Paola Andrea
Pesce, Pablo Adrian
de Matteo, Elena Noemí
García Lombardi, Mercedes
Rey, Guadalupe
Preciado, María Victoria
author Chabay, Paola Andrea
author_facet Chabay, Paola Andrea
Pesce, Pablo Adrian
de Matteo, Elena Noemí
García Lombardi, Mercedes
Rey, Guadalupe
Preciado, María Victoria
author_role author
author2 Pesce, Pablo Adrian
de Matteo, Elena Noemí
García Lombardi, Mercedes
Rey, Guadalupe
Preciado, María Victoria
author2_role author
author
author
author
author
dc.subject.none.fl_str_mv HODGKIN LYMPHOMA
CHILDREN
OUTCOME
topic HODGKIN LYMPHOMA
CHILDREN
OUTCOME
purl_subject.fl_str_mv https://purl.org/becyt/ford/3.5
https://purl.org/becyt/ford/3
dc.description.none.fl_txt_mv In Argentina, lymphomas account for 13.6% of all pediatric tumors and 47% of them are Hodgkin lymphoma. Previous studies of lymphoma series have reported the expression of apoptotic and cell cycle proteins. Our aim was to study these markers in our pediatric patients and correlate them with their outcome. Immunohistochemical staining with monoclonal antibodies anti-p53, bcl-2, p21waf1, and mdm2 were performed on formalin fixed paraffin-embedded Hodgkin lymphoma lymph node biopsies from 54 pediatric patients. The analyzed oncogenes p53, bcl-2, p21waf1, and mdm2 exhibited 81%, 44%, 76%, and 90% positive staining, respectively. The most prevalent p53/p21waf1 expression pattern was p53+/p21waf1+, in 57% of cases, whereas concerning p53/mdm2 expression pattern p53+/mdm2+ was observed in 61% of cases. We failed to .nd any statistically signi.cant correlation between oncogene expression and patient’s survival. It seems that p53 plays an important role in lymphomagenesis in our studied population, because it is overexpressed in 81% of Hodgkin lymphoma cases and in more than 50% of cases, it might be able to activate its cellular e.ectors. Bcl-2 staining observed in 44% of our cases could represent a failure in bcl-2 down-regulation that leads to a rescue event in defective germinal center B-cells, that allows them to develop into Reed-Sternberg and Hodgkin cells.
Fil: Chabay, Paola Andrea. Gobierno de la Ciudad de Buenos Aires. Hospital General de Niños "Ricardo Gutiérrez"; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina
Fil: Pesce, Pablo Adrian. No especifíca;
Fil: de Matteo, Elena Noemí. Gobierno de la Ciudad de Buenos Aires. Hospital General de Niños "Ricardo Gutiérrez"; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina
Fil: García Lombardi, Mercedes. No especifíca;
Fil: Rey, Guadalupe. No especifíca;
Fil: Preciado, María Victoria. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina. Gobierno de la Ciudad de Buenos Aires. Hospital General de Niños "Ricardo Gutiérrez"; Argentina
description In Argentina, lymphomas account for 13.6% of all pediatric tumors and 47% of them are Hodgkin lymphoma. Previous studies of lymphoma series have reported the expression of apoptotic and cell cycle proteins. Our aim was to study these markers in our pediatric patients and correlate them with their outcome. Immunohistochemical staining with monoclonal antibodies anti-p53, bcl-2, p21waf1, and mdm2 were performed on formalin fixed paraffin-embedded Hodgkin lymphoma lymph node biopsies from 54 pediatric patients. The analyzed oncogenes p53, bcl-2, p21waf1, and mdm2 exhibited 81%, 44%, 76%, and 90% positive staining, respectively. The most prevalent p53/p21waf1 expression pattern was p53+/p21waf1+, in 57% of cases, whereas concerning p53/mdm2 expression pattern p53+/mdm2+ was observed in 61% of cases. We failed to .nd any statistically signi.cant correlation between oncogene expression and patient’s survival. It seems that p53 plays an important role in lymphomagenesis in our studied population, because it is overexpressed in 81% of Hodgkin lymphoma cases and in more than 50% of cases, it might be able to activate its cellular e.ectors. Bcl-2 staining observed in 44% of our cases could represent a failure in bcl-2 down-regulation that leads to a rescue event in defective germinal center B-cells, that allows them to develop into Reed-Sternberg and Hodgkin cells.
publishDate 2006
dc.date.none.fl_str_mv 2006-09
dc.type.none.fl_str_mv info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
http://purl.org/coar/resource_type/c_6501
info:ar-repo/semantics/articulo
format article
status_str publishedVersion
dc.identifier.none.fl_str_mv http://hdl.handle.net/11336/245205
Chabay, Paola Andrea; Pesce, Pablo Adrian; de Matteo, Elena Noemí; García Lombardi, Mercedes; Rey, Guadalupe; et al.; No Influence of bcl-2, p53, and p21 waf1 Protein Expression on the Outcome of Pediatric Hodgkin Lymphomas; Lippincott Williams; Journal Of Pediatric Hematology Oncology; 28; 9; 9-2006; 552-558
1077-4114
CONICET Digital
CONICET
url http://hdl.handle.net/11336/245205
identifier_str_mv Chabay, Paola Andrea; Pesce, Pablo Adrian; de Matteo, Elena Noemí; García Lombardi, Mercedes; Rey, Guadalupe; et al.; No Influence of bcl-2, p53, and p21 waf1 Protein Expression on the Outcome of Pediatric Hodgkin Lymphomas; Lippincott Williams; Journal Of Pediatric Hematology Oncology; 28; 9; 9-2006; 552-558
1077-4114
CONICET Digital
CONICET
dc.language.none.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv info:eu-repo/semantics/altIdentifier/url/https://journals.lww.com/jpho-online/abstract/2006/09000/no_influence_of_bcl_2,_p53,_and_p21waf1_protein.2.aspx
info:eu-repo/semantics/altIdentifier/doi/10.1097/01.mph.0000212955.43350.bb
dc.rights.none.fl_str_mv info:eu-repo/semantics/openAccess
https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
eu_rights_str_mv openAccess
rights_invalid_str_mv https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
dc.format.none.fl_str_mv application/pdf
application/pdf
application/pdf
application/pdf
dc.publisher.none.fl_str_mv Lippincott Williams
publisher.none.fl_str_mv Lippincott Williams
dc.source.none.fl_str_mv reponame:CONICET Digital (CONICET)
instname:Consejo Nacional de Investigaciones Científicas y Técnicas
reponame_str CONICET Digital (CONICET)
collection CONICET Digital (CONICET)
instname_str Consejo Nacional de Investigaciones Científicas y Técnicas
repository.name.fl_str_mv CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicas
repository.mail.fl_str_mv dasensio@conicet.gov.ar; lcarlino@conicet.gov.ar
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score 13.070432

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