Diisopropylphenyl-imidazole exerts anthelmintic activity through novel molecular mechanisms

Autores
Blanco, Maria Gabriela; Aletto, Facundo Gastón; Masson, Camila; Vela Gurovic, Maria Soledad; Silbestri, Gustavo Fabián; Garelli, Andres; Rayes, Diego Hernán; de Rosa, Maria Jose
Año de publicación
2018
Idioma
inglés
Tipo de recurso
documento de conferencia
Estado
versión publicada
Descripción
Nematode parasites cause substantial morbidity to billions of people and considerable losses in livestock and food crops. The repertoire of effective anthelmintic compounds for treating these parasitosis is very limited, as drug development has been delayed for decades [1,2]. Moreover, resistance has become a global concern in livestock parasites, and is an emerging issue for human helminthiasis. Parasitic resistance has been reported for all classes of anthelmintics [3,4,5,6]. Therefore, anthelmintics with novel mechanism of action are urgently needed. In this context, the use of non-parasitic nematodes, such as C. elegans, has emerged as a model of parasitic roundworms to test new possible anthelmintics [7,8]. C. elegans is a free-living nematode that shares phylum-specific properties with parasitic roundworms and has been extensively used as an inexpensive, safe and powerful model in biomedical research [9,10]. Therefore, we here screened the nematicidal potential of novel imidazolium and imidazole derivatives, using C. elegans as an established model system. One of these derivatives, diisopropylphenyl-imidazole (DII), is lethal to C. elegans at both mature and immature stages. This lethal effect appears to be specific as DII is harmless to bacteria, Drosophila melanogaster and human cell cultures. Our analysis of DII action on C. elegans mutant strains determined that, in the adult stage, null mutants of unc-29 are completely resistant to the drug. We did this by analyzing the survival of several null mutant worms in presence of the drug diluted in Nematode growth media after 4, 8, 24, 48 and 72 hours of exposure. Muscle expression of this gene completely restores DII sensitivity. UNC-29 has been largely reported as an essential constituent of the levamisole-sensitive muscle nicotinic receptor (L-AChR) [11]. Nevertheless, null mutants in unc-63 and lev-8 (essential and non-essential subunits of L-AChRs, respectively) are as sensitive to DII as the wild-type strain. Therefore, our results suggest that DII effects on adult nematodes rely on a previously unidentified UNC-29-containing muscle AChR, different from the classical L-AChR. Strikingly, DII targets appear to be different between larvae and adults, as unc-29 null mutant larvae are sensitive to the drug. The existence of more than one target could delay resistance development. Its lethality on C. elegans, its harmlessness in non-nematode species and its novel and dual mechanism of action converts DII in a promising candidate compound for anthelmintic therapy.
Fil: Blanco, Maria Gabriela. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Bahía Blanca. Instituto de Investigaciones Bioquímicas de Bahía Blanca. Universidad Nacional del Sur. Instituto de Investigaciones Bioquímicas de Bahía Blanca; Argentina. Universidad Nacional del Sur. Departamento de Biología, Bioquímica y Farmacia; Argentina
Fil: Aletto, Facundo Gastón. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Bahía Blanca. Instituto de Investigaciones Bioquímicas de Bahía Blanca. Universidad Nacional del Sur. Instituto de Investigaciones Bioquímicas de Bahía Blanca; Argentina
Fil: Masson, Camila. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Bahía Blanca. Instituto de Investigaciones Bioquímicas de Bahía Blanca. Universidad Nacional del Sur. Instituto de Investigaciones Bioquímicas de Bahía Blanca; Argentina
Fil: Vela Gurovic, Maria Soledad. Universidad Nacional del Sur. Departamento de Biología, Bioquímica y Farmacia; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Bahía Blanca. Centro de Recursos Naturales Renovables de la Zona Semiárida. Universidad Nacional del Sur. Centro de Recursos Naturales Renovables de la Zona Semiárida; Argentina
Fil: Silbestri, Gustavo Fabián. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Bahía Blanca. Instituto de Química del Sur. Universidad Nacional del Sur. Departamento de Química. Instituto de Química del Sur; Argentina
Fil: Garelli, Andres. Universidad Nacional del Sur. Departamento de Biología, Bioquímica y Farmacia; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Bahía Blanca. Instituto de Investigaciones Bioquímicas de Bahía Blanca. Universidad Nacional del Sur. Instituto de Investigaciones Bioquímicas de Bahía Blanca; Argentina
Fil: Rayes, Diego Hernán. Universidad Nacional del Sur. Departamento de Biología, Bioquímica y Farmacia; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Bahía Blanca. Instituto de Investigaciones Bioquímicas de Bahía Blanca. Universidad Nacional del Sur. Instituto de Investigaciones Bioquímicas de Bahía Blanca; Argentina
Fil: de Rosa, Maria Jose. Universidad Nacional del Sur. Departamento de Biología, Bioquímica y Farmacia; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Bahía Blanca. Instituto de Investigaciones Bioquímicas de Bahía Blanca. Universidad Nacional del Sur. Instituto de Investigaciones Bioquímicas de Bahía Blanca; Argentina
4th Scientific Meeting of the Research Network Natural Products against Neglected Diseases
Buenos Aires
Argentina
Universidad de Buenos Aires
Materia
IMIDAZOLE-DERIVATIVES
SCREENING
ANTHELMINTIC
ACETILCOLINE RECEPTOR
CAENORHABDITIS ELEGANS
Nivel de accesibilidad
acceso abierto
Condiciones de uso
https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
Repositorio
CONICET Digital (CONICET)
Institución
Consejo Nacional de Investigaciones Científicas y Técnicas
OAI Identificador
oai:ri.conicet.gov.ar:11336/230690
Acceder
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repository_id_str 3498
network_name_str CONICET Digital (CONICET)
spelling Diisopropylphenyl-imidazole exerts anthelmintic activity through novel molecular mechanismsBlanco, Maria GabrielaAletto, Facundo GastónMasson, CamilaVela Gurovic, Maria SoledadSilbestri, Gustavo FabiánGarelli, AndresRayes, Diego Hernánde Rosa, Maria JoseIMIDAZOLE-DERIVATIVESSCREENINGANTHELMINTICACETILCOLINE RECEPTORCAENORHABDITIS ELEGANShttps://purl.org/becyt/ford/3.1https://purl.org/becyt/ford/3Nematode parasites cause substantial morbidity to billions of people and considerable losses in livestock and food crops. The repertoire of effective anthelmintic compounds for treating these parasitosis is very limited, as drug development has been delayed for decades [1,2]. Moreover, resistance has become a global concern in livestock parasites, and is an emerging issue for human helminthiasis. Parasitic resistance has been reported for all classes of anthelmintics [3,4,5,6]. Therefore, anthelmintics with novel mechanism of action are urgently needed. In this context, the use of non-parasitic nematodes, such as C. elegans, has emerged as a model of parasitic roundworms to test new possible anthelmintics [7,8]. C. elegans is a free-living nematode that shares phylum-specific properties with parasitic roundworms and has been extensively used as an inexpensive, safe and powerful model in biomedical research [9,10]. Therefore, we here screened the nematicidal potential of novel imidazolium and imidazole derivatives, using C. elegans as an established model system. One of these derivatives, diisopropylphenyl-imidazole (DII), is lethal to C. elegans at both mature and immature stages. This lethal effect appears to be specific as DII is harmless to bacteria, Drosophila melanogaster and human cell cultures. Our analysis of DII action on C. elegans mutant strains determined that, in the adult stage, null mutants of unc-29 are completely resistant to the drug. We did this by analyzing the survival of several null mutant worms in presence of the drug diluted in Nematode growth media after 4, 8, 24, 48 and 72 hours of exposure. Muscle expression of this gene completely restores DII sensitivity. UNC-29 has been largely reported as an essential constituent of the levamisole-sensitive muscle nicotinic receptor (L-AChR) [11]. Nevertheless, null mutants in unc-63 and lev-8 (essential and non-essential subunits of L-AChRs, respectively) are as sensitive to DII as the wild-type strain. Therefore, our results suggest that DII effects on adult nematodes rely on a previously unidentified UNC-29-containing muscle AChR, different from the classical L-AChR. Strikingly, DII targets appear to be different between larvae and adults, as unc-29 null mutant larvae are sensitive to the drug. The existence of more than one target could delay resistance development. Its lethality on C. elegans, its harmlessness in non-nematode species and its novel and dual mechanism of action converts DII in a promising candidate compound for anthelmintic therapy.Fil: Blanco, Maria Gabriela. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Bahía Blanca. Instituto de Investigaciones Bioquímicas de Bahía Blanca. Universidad Nacional del Sur. Instituto de Investigaciones Bioquímicas de Bahía Blanca; Argentina. Universidad Nacional del Sur. Departamento de Biología, Bioquímica y Farmacia; ArgentinaFil: Aletto, Facundo Gastón. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Bahía Blanca. Instituto de Investigaciones Bioquímicas de Bahía Blanca. Universidad Nacional del Sur. Instituto de Investigaciones Bioquímicas de Bahía Blanca; ArgentinaFil: Masson, Camila. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Bahía Blanca. Instituto de Investigaciones Bioquímicas de Bahía Blanca. Universidad Nacional del Sur. Instituto de Investigaciones Bioquímicas de Bahía Blanca; ArgentinaFil: Vela Gurovic, Maria Soledad. Universidad Nacional del Sur. Departamento de Biología, Bioquímica y Farmacia; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Bahía Blanca. Centro de Recursos Naturales Renovables de la Zona Semiárida. Universidad Nacional del Sur. Centro de Recursos Naturales Renovables de la Zona Semiárida; ArgentinaFil: Silbestri, Gustavo Fabián. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Bahía Blanca. Instituto de Química del Sur. Universidad Nacional del Sur. Departamento de Química. Instituto de Química del Sur; ArgentinaFil: Garelli, Andres. Universidad Nacional del Sur. Departamento de Biología, Bioquímica y Farmacia; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Bahía Blanca. Instituto de Investigaciones Bioquímicas de Bahía Blanca. Universidad Nacional del Sur. Instituto de Investigaciones Bioquímicas de Bahía Blanca; ArgentinaFil: Rayes, Diego Hernán. Universidad Nacional del Sur. Departamento de Biología, Bioquímica y Farmacia; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Bahía Blanca. Instituto de Investigaciones Bioquímicas de Bahía Blanca. Universidad Nacional del Sur. Instituto de Investigaciones Bioquímicas de Bahía Blanca; ArgentinaFil: de Rosa, Maria Jose. Universidad Nacional del Sur. Departamento de Biología, Bioquímica y Farmacia; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Bahía Blanca. Instituto de Investigaciones Bioquímicas de Bahía Blanca. Universidad Nacional del Sur. Instituto de Investigaciones Bioquímicas de Bahía Blanca; Argentina4th Scientific Meeting of the Research Network Natural Products against Neglected DiseasesBuenos AiresArgentinaUniversidad de Buenos AiresUniversidad de Buenos Aires. Facultad de Farmacia y Bioquímica. Instituto de Química y Metabolismo del FármacoSülsen, Valeria PatriciaRedko, Flavia del Carmen2018info:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/conferenceObjectCongresoBookhttp://purl.org/coar/resource_type/c_5794info:ar-repo/semantics/documentoDeConferenciaapplication/pdfapplication/pdfapplication/pdfapplication/pdfhttp://hdl.handle.net/11336/230690Diisopropylphenyl-imidazole exerts anthelmintic activity through novel molecular mechanisms; 4th Scientific Meeting of the Research Network Natural Products against Neglected Diseases; Buenos Aires; Argentina; 2018; 210-211978-987-47034-0-8CONICET DigitalCONICETenginfo:eu-repo/semantics/altIdentifier/url/https://iquimefa.conicet.gov.ar/Internacionalinfo:eu-repo/semantics/openAccesshttps://creativecommons.org/licenses/by-nc-sa/2.5/ar/reponame:CONICET Digital (CONICET)instname:Consejo Nacional de Investigaciones Científicas y Técnicas2025-09-29T09:38:52Zoai:ri.conicet.gov.ar:11336/230690instacron:CONICETInstitucionalhttp://ri.conicet.gov.ar/Organismo científico-tecnológicoNo correspondehttp://ri.conicet.gov.ar/oai/requestdasensio@conicet.gov.ar; lcarlino@conicet.gov.arArgentinaNo correspondeNo correspondeNo correspondeopendoar:34982025-09-29 09:38:52.972CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicasfalse
dc.title.none.fl_str_mv Diisopropylphenyl-imidazole exerts anthelmintic activity through novel molecular mechanisms
title Diisopropylphenyl-imidazole exerts anthelmintic activity through novel molecular mechanisms
spellingShingle Diisopropylphenyl-imidazole exerts anthelmintic activity through novel molecular mechanisms
Blanco, Maria Gabriela
IMIDAZOLE-DERIVATIVES
SCREENING
ANTHELMINTIC
ACETILCOLINE RECEPTOR
CAENORHABDITIS ELEGANS
title_short Diisopropylphenyl-imidazole exerts anthelmintic activity through novel molecular mechanisms
title_full Diisopropylphenyl-imidazole exerts anthelmintic activity through novel molecular mechanisms
title_fullStr Diisopropylphenyl-imidazole exerts anthelmintic activity through novel molecular mechanisms
title_full_unstemmed Diisopropylphenyl-imidazole exerts anthelmintic activity through novel molecular mechanisms
title_sort Diisopropylphenyl-imidazole exerts anthelmintic activity through novel molecular mechanisms
dc.creator.none.fl_str_mv Blanco, Maria Gabriela
Aletto, Facundo Gastón
Masson, Camila
Vela Gurovic, Maria Soledad
Silbestri, Gustavo Fabián
Garelli, Andres
Rayes, Diego Hernán
de Rosa, Maria Jose
author Blanco, Maria Gabriela
author_facet Blanco, Maria Gabriela
Aletto, Facundo Gastón
Masson, Camila
Vela Gurovic, Maria Soledad
Silbestri, Gustavo Fabián
Garelli, Andres
Rayes, Diego Hernán
de Rosa, Maria Jose
author_role author
author2 Aletto, Facundo Gastón
Masson, Camila
Vela Gurovic, Maria Soledad
Silbestri, Gustavo Fabián
Garelli, Andres
Rayes, Diego Hernán
de Rosa, Maria Jose
author2_role author
author
author
author
author
author
author
dc.contributor.none.fl_str_mv Sülsen, Valeria Patricia
Redko, Flavia del Carmen
dc.subject.none.fl_str_mv IMIDAZOLE-DERIVATIVES
SCREENING
ANTHELMINTIC
ACETILCOLINE RECEPTOR
CAENORHABDITIS ELEGANS
topic IMIDAZOLE-DERIVATIVES
SCREENING
ANTHELMINTIC
ACETILCOLINE RECEPTOR
CAENORHABDITIS ELEGANS
purl_subject.fl_str_mv https://purl.org/becyt/ford/3.1
https://purl.org/becyt/ford/3
dc.description.none.fl_txt_mv Nematode parasites cause substantial morbidity to billions of people and considerable losses in livestock and food crops. The repertoire of effective anthelmintic compounds for treating these parasitosis is very limited, as drug development has been delayed for decades [1,2]. Moreover, resistance has become a global concern in livestock parasites, and is an emerging issue for human helminthiasis. Parasitic resistance has been reported for all classes of anthelmintics [3,4,5,6]. Therefore, anthelmintics with novel mechanism of action are urgently needed. In this context, the use of non-parasitic nematodes, such as C. elegans, has emerged as a model of parasitic roundworms to test new possible anthelmintics [7,8]. C. elegans is a free-living nematode that shares phylum-specific properties with parasitic roundworms and has been extensively used as an inexpensive, safe and powerful model in biomedical research [9,10]. Therefore, we here screened the nematicidal potential of novel imidazolium and imidazole derivatives, using C. elegans as an established model system. One of these derivatives, diisopropylphenyl-imidazole (DII), is lethal to C. elegans at both mature and immature stages. This lethal effect appears to be specific as DII is harmless to bacteria, Drosophila melanogaster and human cell cultures. Our analysis of DII action on C. elegans mutant strains determined that, in the adult stage, null mutants of unc-29 are completely resistant to the drug. We did this by analyzing the survival of several null mutant worms in presence of the drug diluted in Nematode growth media after 4, 8, 24, 48 and 72 hours of exposure. Muscle expression of this gene completely restores DII sensitivity. UNC-29 has been largely reported as an essential constituent of the levamisole-sensitive muscle nicotinic receptor (L-AChR) [11]. Nevertheless, null mutants in unc-63 and lev-8 (essential and non-essential subunits of L-AChRs, respectively) are as sensitive to DII as the wild-type strain. Therefore, our results suggest that DII effects on adult nematodes rely on a previously unidentified UNC-29-containing muscle AChR, different from the classical L-AChR. Strikingly, DII targets appear to be different between larvae and adults, as unc-29 null mutant larvae are sensitive to the drug. The existence of more than one target could delay resistance development. Its lethality on C. elegans, its harmlessness in non-nematode species and its novel and dual mechanism of action converts DII in a promising candidate compound for anthelmintic therapy.
Fil: Blanco, Maria Gabriela. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Bahía Blanca. Instituto de Investigaciones Bioquímicas de Bahía Blanca. Universidad Nacional del Sur. Instituto de Investigaciones Bioquímicas de Bahía Blanca; Argentina. Universidad Nacional del Sur. Departamento de Biología, Bioquímica y Farmacia; Argentina
Fil: Aletto, Facundo Gastón. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Bahía Blanca. Instituto de Investigaciones Bioquímicas de Bahía Blanca. Universidad Nacional del Sur. Instituto de Investigaciones Bioquímicas de Bahía Blanca; Argentina
Fil: Masson, Camila. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Bahía Blanca. Instituto de Investigaciones Bioquímicas de Bahía Blanca. Universidad Nacional del Sur. Instituto de Investigaciones Bioquímicas de Bahía Blanca; Argentina
Fil: Vela Gurovic, Maria Soledad. Universidad Nacional del Sur. Departamento de Biología, Bioquímica y Farmacia; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Bahía Blanca. Centro de Recursos Naturales Renovables de la Zona Semiárida. Universidad Nacional del Sur. Centro de Recursos Naturales Renovables de la Zona Semiárida; Argentina
Fil: Silbestri, Gustavo Fabián. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Bahía Blanca. Instituto de Química del Sur. Universidad Nacional del Sur. Departamento de Química. Instituto de Química del Sur; Argentina
Fil: Garelli, Andres. Universidad Nacional del Sur. Departamento de Biología, Bioquímica y Farmacia; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Bahía Blanca. Instituto de Investigaciones Bioquímicas de Bahía Blanca. Universidad Nacional del Sur. Instituto de Investigaciones Bioquímicas de Bahía Blanca; Argentina
Fil: Rayes, Diego Hernán. Universidad Nacional del Sur. Departamento de Biología, Bioquímica y Farmacia; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Bahía Blanca. Instituto de Investigaciones Bioquímicas de Bahía Blanca. Universidad Nacional del Sur. Instituto de Investigaciones Bioquímicas de Bahía Blanca; Argentina
Fil: de Rosa, Maria Jose. Universidad Nacional del Sur. Departamento de Biología, Bioquímica y Farmacia; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Bahía Blanca. Instituto de Investigaciones Bioquímicas de Bahía Blanca. Universidad Nacional del Sur. Instituto de Investigaciones Bioquímicas de Bahía Blanca; Argentina
4th Scientific Meeting of the Research Network Natural Products against Neglected Diseases
Buenos Aires
Argentina
Universidad de Buenos Aires
description Nematode parasites cause substantial morbidity to billions of people and considerable losses in livestock and food crops. The repertoire of effective anthelmintic compounds for treating these parasitosis is very limited, as drug development has been delayed for decades [1,2]. Moreover, resistance has become a global concern in livestock parasites, and is an emerging issue for human helminthiasis. Parasitic resistance has been reported for all classes of anthelmintics [3,4,5,6]. Therefore, anthelmintics with novel mechanism of action are urgently needed. In this context, the use of non-parasitic nematodes, such as C. elegans, has emerged as a model of parasitic roundworms to test new possible anthelmintics [7,8]. C. elegans is a free-living nematode that shares phylum-specific properties with parasitic roundworms and has been extensively used as an inexpensive, safe and powerful model in biomedical research [9,10]. Therefore, we here screened the nematicidal potential of novel imidazolium and imidazole derivatives, using C. elegans as an established model system. One of these derivatives, diisopropylphenyl-imidazole (DII), is lethal to C. elegans at both mature and immature stages. This lethal effect appears to be specific as DII is harmless to bacteria, Drosophila melanogaster and human cell cultures. Our analysis of DII action on C. elegans mutant strains determined that, in the adult stage, null mutants of unc-29 are completely resistant to the drug. We did this by analyzing the survival of several null mutant worms in presence of the drug diluted in Nematode growth media after 4, 8, 24, 48 and 72 hours of exposure. Muscle expression of this gene completely restores DII sensitivity. UNC-29 has been largely reported as an essential constituent of the levamisole-sensitive muscle nicotinic receptor (L-AChR) [11]. Nevertheless, null mutants in unc-63 and lev-8 (essential and non-essential subunits of L-AChRs, respectively) are as sensitive to DII as the wild-type strain. Therefore, our results suggest that DII effects on adult nematodes rely on a previously unidentified UNC-29-containing muscle AChR, different from the classical L-AChR. Strikingly, DII targets appear to be different between larvae and adults, as unc-29 null mutant larvae are sensitive to the drug. The existence of more than one target could delay resistance development. Its lethality on C. elegans, its harmlessness in non-nematode species and its novel and dual mechanism of action converts DII in a promising candidate compound for anthelmintic therapy.
publishDate 2018
dc.date.none.fl_str_mv 2018
dc.type.none.fl_str_mv info:eu-repo/semantics/publishedVersion
info:eu-repo/semantics/conferenceObject
Congreso
Book
http://purl.org/coar/resource_type/c_5794
info:ar-repo/semantics/documentoDeConferencia
status_str publishedVersion
format conferenceObject
dc.identifier.none.fl_str_mv http://hdl.handle.net/11336/230690
Diisopropylphenyl-imidazole exerts anthelmintic activity through novel molecular mechanisms; 4th Scientific Meeting of the Research Network Natural Products against Neglected Diseases; Buenos Aires; Argentina; 2018; 210-211
978-987-47034-0-8
CONICET Digital
CONICET
url http://hdl.handle.net/11336/230690
identifier_str_mv Diisopropylphenyl-imidazole exerts anthelmintic activity through novel molecular mechanisms; 4th Scientific Meeting of the Research Network Natural Products against Neglected Diseases; Buenos Aires; Argentina; 2018; 210-211
978-987-47034-0-8
CONICET Digital
CONICET
dc.language.none.fl_str_mv eng
language eng
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dc.coverage.none.fl_str_mv Internacional
dc.publisher.none.fl_str_mv Universidad de Buenos Aires. Facultad de Farmacia y Bioquímica. Instituto de Química y Metabolismo del Fármaco
publisher.none.fl_str_mv Universidad de Buenos Aires. Facultad de Farmacia y Bioquímica. Instituto de Química y Metabolismo del Fármaco
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repository.mail.fl_str_mv dasensio@conicet.gov.ar; lcarlino@conicet.gov.ar
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