Short-Term Estivation and Hibernation Induce Changes in the Blood and Circulating Hemocytes of the Apple Snail Pomacea canaliculata

Autores
Rodríguez, Cristian; Campoy Díaz, Alejandra Daniela; Giraud Billoud, Maximiliano German
Año de publicación
2023
Idioma
inglés
Tipo de recurso
artículo
Estado
versión publicada
Descripción
States of natural dormancy include estivation and hibernation. Ampullariids are exemplarybecause they undergo estivation when deprived of water or hibernation when exposed to very lowtemperatures. Regardless of the condition, ampullariids show increased endogenous antioxidantdefenses, anticipating the expected respiratory burst during reoxygenation after reactivation, knownas ?Preparation for Oxidative Stress (POS)?. In this work, we tested the POS hypothesis for changesin the blood and hemocytes of the bimodal breather Pomacea canaliculata (Ampullariidae) induced atexperimental estivation and hibernation. We described respiratory (hemocyanin, proteins, lactate),antioxidant (GSH, uric acid, SOD, CAT, GST), and immunological (hemocyte levels, ROS production)parameters. We showed that, although the protein level remains unchanged in all experimentalgroups, hemocyanin increases in response to estivation. Furthermore, lactate remains unchangedin challenged snails, suggesting an aerobic metabolism during short-term challenges. Blood uricacid increases during estivation and arousal from estivation or hibernation, supporting the previouslyproposed antioxidant role. Regarding hemocytes, we showed that the total populationincreases with all challenges, and granulocytes increase during hibernation. We further showed thathibernation affects ROS production by hemocytes, possibly through mitochondrial inhibition. Thisstudy contributed to the knowledge of the adaptive strategies of ampullariids to tolerate adverseenvironmental conditions.
Fil: Rodríguez, Cristian. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Mendoza. Instituto de Histología y Embriología de Mendoza Dr. Mario H. Burgos. Universidad Nacional de Cuyo. Facultad de Ciencias Médicas. Instituto de Histología y Embriología de Mendoza Dr. Mario H. Burgos; Argentina
Fil: Campoy Díaz, Alejandra Daniela. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Mendoza. Instituto de Histología y Embriología de Mendoza Dr. Mario H. Burgos. Universidad Nacional de Cuyo. Facultad de Ciencias Médicas. Instituto de Histología y Embriología de Mendoza Dr. Mario H. Burgos; Argentina. Universidad Nacional de Cuyo. Facultad de Ciencias Exactas y Naturales. Departamento de Biología; Argentina
Fil: Giraud Billoud, Maximiliano German. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Mendoza. Instituto de Histología y Embriología de Mendoza Dr. Mario H. Burgos. Universidad Nacional de Cuyo. Facultad de Ciencias Médicas. Instituto de Histología y Embriología de Mendoza Dr. Mario H. Burgos; Argentina
Materia
Oxidative stress defenses
hypometabolism
immune system
hemocyanin
Nivel de accesibilidad
acceso abierto
Condiciones de uso
https://creativecommons.org/licenses/by/2.5/ar/
Repositorio
CONICET Digital (CONICET)
Institución
Consejo Nacional de Investigaciones Científicas y Técnicas
OAI Identificador
oai:ri.conicet.gov.ar:11336/230476
Acceder
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oai_identifier_str oai:ri.conicet.gov.ar:11336/230476
network_acronym_str CONICETDig
repository_id_str 3498
network_name_str CONICET Digital (CONICET)
spelling Short-Term Estivation and Hibernation Induce Changes in the Blood and Circulating Hemocytes of the Apple Snail Pomacea canaliculataRodríguez, CristianCampoy Díaz, Alejandra DanielaGiraud Billoud, Maximiliano GermanOxidative stress defenseshypometabolismimmune systemhemocyaninhttps://purl.org/becyt/ford/1.6https://purl.org/becyt/ford/1States of natural dormancy include estivation and hibernation. Ampullariids are exemplarybecause they undergo estivation when deprived of water or hibernation when exposed to very lowtemperatures. Regardless of the condition, ampullariids show increased endogenous antioxidantdefenses, anticipating the expected respiratory burst during reoxygenation after reactivation, knownas ?Preparation for Oxidative Stress (POS)?. In this work, we tested the POS hypothesis for changesin the blood and hemocytes of the bimodal breather Pomacea canaliculata (Ampullariidae) induced atexperimental estivation and hibernation. We described respiratory (hemocyanin, proteins, lactate),antioxidant (GSH, uric acid, SOD, CAT, GST), and immunological (hemocyte levels, ROS production)parameters. We showed that, although the protein level remains unchanged in all experimentalgroups, hemocyanin increases in response to estivation. Furthermore, lactate remains unchangedin challenged snails, suggesting an aerobic metabolism during short-term challenges. Blood uricacid increases during estivation and arousal from estivation or hibernation, supporting the previouslyproposed antioxidant role. Regarding hemocytes, we showed that the total populationincreases with all challenges, and granulocytes increase during hibernation. We further showed thathibernation affects ROS production by hemocytes, possibly through mitochondrial inhibition. Thisstudy contributed to the knowledge of the adaptive strategies of ampullariids to tolerate adverseenvironmental conditions.Fil: Rodríguez, Cristian. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Mendoza. Instituto de Histología y Embriología de Mendoza Dr. Mario H. Burgos. Universidad Nacional de Cuyo. Facultad de Ciencias Médicas. Instituto de Histología y Embriología de Mendoza Dr. Mario H. Burgos; ArgentinaFil: Campoy Díaz, Alejandra Daniela. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Mendoza. Instituto de Histología y Embriología de Mendoza Dr. Mario H. Burgos. Universidad Nacional de Cuyo. Facultad de Ciencias Médicas. Instituto de Histología y Embriología de Mendoza Dr. Mario H. Burgos; Argentina. Universidad Nacional de Cuyo. Facultad de Ciencias Exactas y Naturales. Departamento de Biología; ArgentinaFil: Giraud Billoud, Maximiliano German. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Mendoza. Instituto de Histología y Embriología de Mendoza Dr. Mario H. Burgos. Universidad Nacional de Cuyo. Facultad de Ciencias Médicas. Instituto de Histología y Embriología de Mendoza Dr. Mario H. Burgos; ArgentinaMDPI2023-02info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionhttp://purl.org/coar/resource_type/c_6501info:ar-repo/semantics/articuloapplication/pdfapplication/pdfapplication/pdfhttp://hdl.handle.net/11336/230476Rodríguez, Cristian; Campoy Díaz, Alejandra Daniela; Giraud Billoud, Maximiliano German; Short-Term Estivation and Hibernation Induce Changes in the Blood and Circulating Hemocytes of the Apple Snail Pomacea canaliculata; MDPI; Metabolites; 13; 2; 2-2023; 289-3022218-1989CONICET DigitalCONICETenginfo:eu-repo/semantics/altIdentifier/url/https://www.mdpi.com/2218-1989/13/2/289info:eu-repo/semantics/altIdentifier/doi/10.3390/metabo13020289info:eu-repo/semantics/openAccesshttps://creativecommons.org/licenses/by/2.5/ar/reponame:CONICET Digital (CONICET)instname:Consejo Nacional de Investigaciones Científicas y Técnicas2025-10-22T11:12:08Zoai:ri.conicet.gov.ar:11336/230476instacron:CONICETInstitucionalhttp://ri.conicet.gov.ar/Organismo científico-tecnológicoNo correspondehttp://ri.conicet.gov.ar/oai/requestdasensio@conicet.gov.ar; lcarlino@conicet.gov.arArgentinaNo correspondeNo correspondeNo correspondeopendoar:34982025-10-22 11:12:09.022CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicasfalse
dc.title.none.fl_str_mv Short-Term Estivation and Hibernation Induce Changes in the Blood and Circulating Hemocytes of the Apple Snail Pomacea canaliculata
title Short-Term Estivation and Hibernation Induce Changes in the Blood and Circulating Hemocytes of the Apple Snail Pomacea canaliculata
spellingShingle Short-Term Estivation and Hibernation Induce Changes in the Blood and Circulating Hemocytes of the Apple Snail Pomacea canaliculata
Rodríguez, Cristian
Oxidative stress defenses
hypometabolism
immune system
hemocyanin
title_short Short-Term Estivation and Hibernation Induce Changes in the Blood and Circulating Hemocytes of the Apple Snail Pomacea canaliculata
title_full Short-Term Estivation and Hibernation Induce Changes in the Blood and Circulating Hemocytes of the Apple Snail Pomacea canaliculata
title_fullStr Short-Term Estivation and Hibernation Induce Changes in the Blood and Circulating Hemocytes of the Apple Snail Pomacea canaliculata
title_full_unstemmed Short-Term Estivation and Hibernation Induce Changes in the Blood and Circulating Hemocytes of the Apple Snail Pomacea canaliculata
title_sort Short-Term Estivation and Hibernation Induce Changes in the Blood and Circulating Hemocytes of the Apple Snail Pomacea canaliculata
dc.creator.none.fl_str_mv Rodríguez, Cristian
Campoy Díaz, Alejandra Daniela
Giraud Billoud, Maximiliano German
author Rodríguez, Cristian
author_facet Rodríguez, Cristian
Campoy Díaz, Alejandra Daniela
Giraud Billoud, Maximiliano German
author_role author
author2 Campoy Díaz, Alejandra Daniela
Giraud Billoud, Maximiliano German
author2_role author
author
dc.subject.none.fl_str_mv Oxidative stress defenses
hypometabolism
immune system
hemocyanin
topic Oxidative stress defenses
hypometabolism
immune system
hemocyanin
purl_subject.fl_str_mv https://purl.org/becyt/ford/1.6
https://purl.org/becyt/ford/1
dc.description.none.fl_txt_mv States of natural dormancy include estivation and hibernation. Ampullariids are exemplarybecause they undergo estivation when deprived of water or hibernation when exposed to very lowtemperatures. Regardless of the condition, ampullariids show increased endogenous antioxidantdefenses, anticipating the expected respiratory burst during reoxygenation after reactivation, knownas ?Preparation for Oxidative Stress (POS)?. In this work, we tested the POS hypothesis for changesin the blood and hemocytes of the bimodal breather Pomacea canaliculata (Ampullariidae) induced atexperimental estivation and hibernation. We described respiratory (hemocyanin, proteins, lactate),antioxidant (GSH, uric acid, SOD, CAT, GST), and immunological (hemocyte levels, ROS production)parameters. We showed that, although the protein level remains unchanged in all experimentalgroups, hemocyanin increases in response to estivation. Furthermore, lactate remains unchangedin challenged snails, suggesting an aerobic metabolism during short-term challenges. Blood uricacid increases during estivation and arousal from estivation or hibernation, supporting the previouslyproposed antioxidant role. Regarding hemocytes, we showed that the total populationincreases with all challenges, and granulocytes increase during hibernation. We further showed thathibernation affects ROS production by hemocytes, possibly through mitochondrial inhibition. Thisstudy contributed to the knowledge of the adaptive strategies of ampullariids to tolerate adverseenvironmental conditions.
Fil: Rodríguez, Cristian. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Mendoza. Instituto de Histología y Embriología de Mendoza Dr. Mario H. Burgos. Universidad Nacional de Cuyo. Facultad de Ciencias Médicas. Instituto de Histología y Embriología de Mendoza Dr. Mario H. Burgos; Argentina
Fil: Campoy Díaz, Alejandra Daniela. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Mendoza. Instituto de Histología y Embriología de Mendoza Dr. Mario H. Burgos. Universidad Nacional de Cuyo. Facultad de Ciencias Médicas. Instituto de Histología y Embriología de Mendoza Dr. Mario H. Burgos; Argentina. Universidad Nacional de Cuyo. Facultad de Ciencias Exactas y Naturales. Departamento de Biología; Argentina
Fil: Giraud Billoud, Maximiliano German. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Mendoza. Instituto de Histología y Embriología de Mendoza Dr. Mario H. Burgos. Universidad Nacional de Cuyo. Facultad de Ciencias Médicas. Instituto de Histología y Embriología de Mendoza Dr. Mario H. Burgos; Argentina
description States of natural dormancy include estivation and hibernation. Ampullariids are exemplarybecause they undergo estivation when deprived of water or hibernation when exposed to very lowtemperatures. Regardless of the condition, ampullariids show increased endogenous antioxidantdefenses, anticipating the expected respiratory burst during reoxygenation after reactivation, knownas ?Preparation for Oxidative Stress (POS)?. In this work, we tested the POS hypothesis for changesin the blood and hemocytes of the bimodal breather Pomacea canaliculata (Ampullariidae) induced atexperimental estivation and hibernation. We described respiratory (hemocyanin, proteins, lactate),antioxidant (GSH, uric acid, SOD, CAT, GST), and immunological (hemocyte levels, ROS production)parameters. We showed that, although the protein level remains unchanged in all experimentalgroups, hemocyanin increases in response to estivation. Furthermore, lactate remains unchangedin challenged snails, suggesting an aerobic metabolism during short-term challenges. Blood uricacid increases during estivation and arousal from estivation or hibernation, supporting the previouslyproposed antioxidant role. Regarding hemocytes, we showed that the total populationincreases with all challenges, and granulocytes increase during hibernation. We further showed thathibernation affects ROS production by hemocytes, possibly through mitochondrial inhibition. Thisstudy contributed to the knowledge of the adaptive strategies of ampullariids to tolerate adverseenvironmental conditions.
publishDate 2023
dc.date.none.fl_str_mv 2023-02
dc.type.none.fl_str_mv info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
http://purl.org/coar/resource_type/c_6501
info:ar-repo/semantics/articulo
format article
status_str publishedVersion
dc.identifier.none.fl_str_mv http://hdl.handle.net/11336/230476
Rodríguez, Cristian; Campoy Díaz, Alejandra Daniela; Giraud Billoud, Maximiliano German; Short-Term Estivation and Hibernation Induce Changes in the Blood and Circulating Hemocytes of the Apple Snail Pomacea canaliculata; MDPI; Metabolites; 13; 2; 2-2023; 289-302
2218-1989
CONICET Digital
CONICET
url http://hdl.handle.net/11336/230476
identifier_str_mv Rodríguez, Cristian; Campoy Díaz, Alejandra Daniela; Giraud Billoud, Maximiliano German; Short-Term Estivation and Hibernation Induce Changes in the Blood and Circulating Hemocytes of the Apple Snail Pomacea canaliculata; MDPI; Metabolites; 13; 2; 2-2023; 289-302
2218-1989
CONICET Digital
CONICET
dc.language.none.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv info:eu-repo/semantics/altIdentifier/url/https://www.mdpi.com/2218-1989/13/2/289
info:eu-repo/semantics/altIdentifier/doi/10.3390/metabo13020289
dc.rights.none.fl_str_mv info:eu-repo/semantics/openAccess
https://creativecommons.org/licenses/by/2.5/ar/
eu_rights_str_mv openAccess
rights_invalid_str_mv https://creativecommons.org/licenses/by/2.5/ar/
dc.format.none.fl_str_mv application/pdf
application/pdf
application/pdf
dc.publisher.none.fl_str_mv MDPI
publisher.none.fl_str_mv MDPI
dc.source.none.fl_str_mv reponame:CONICET Digital (CONICET)
instname:Consejo Nacional de Investigaciones Científicas y Técnicas
reponame_str CONICET Digital (CONICET)
collection CONICET Digital (CONICET)
instname_str Consejo Nacional de Investigaciones Científicas y Técnicas
repository.name.fl_str_mv CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicas
repository.mail.fl_str_mv dasensio@conicet.gov.ar; lcarlino@conicet.gov.ar
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score 12.982451

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