Effect of Sanitizers and Glucose on Clostridium perfringens Biofilm Formation and Growth

Autores
Castro, Melina Gabriela; Icazatti Zuñiga, Alejandro Ariel; Divizia, Maria Julia; Vega, Alba Edith; Cortiñas, Teresa Inés; Stagnitta, Patricia Virginia
Año de publicación
2016
Idioma
inglés
Tipo de recurso
artículo
Estado
versión publicada
Descripción
Clostridium perfringens enterotoxigenic strain is one of the responsible of food poisoning; this is caused by cpe enterotoxin. Cpe is produced during sporulation, a resistance mechanism. It is described that C. perfringens can form biofilm under stressed environments and this depends of carbohydrates availability. The aim of this work was to evaluate the ability of enteroxigenic (E+) and no enterotoxigenic (E-) C. perfringens strains to form biofilm at different concentrations of carbon source and the inhibitory effect of various Poli P over biofilm formation and the expression of cpe and ccpa genes. Our results indicate that E+ C. perfringens biofilm reach the more complex structure with high glucose concentrations. Even more current sanitizers used in food industry like SAPP and TSPP, showed to stimulate biofilm formation and to inhibit the growth of planktonic cells. On the other hand, morphological changes were observed during biofilm formation under low glucose concentration and in presence of sanitizers. We propose that the change from a bacillary to coccoid form is related to a resistance to stressed environments. Finally the cpe expression is not related to biofilm formation.
Fil: Castro, Melina Gabriela. Universidad Nacional de San Luis; Argentina
Fil: Icazatti Zuñiga, Alejandro Ariel. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - San Luis. Instituto de Matemática Aplicada de San Luis ; Argentina
Fil: Divizia, Maria Julia. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - San Luis. Instituto Multidisciplinario de Investigaciones Biológicas de San Luis. Universidad Nacional de San Luis. Facultad de Ciencias Físico Matemáticas y Naturales. Instituto Multidisciplinario de Investigaciones Biológicas de San Luis; Argentina
Fil: Vega, Alba Edith. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - San Luis. Instituto Multidisciplinario de Investigaciones Biológicas de San Luis. Universidad Nacional de San Luis. Facultad de Ciencias Físico Matemáticas y Naturales. Instituto Multidisciplinario de Investigaciones Biológicas de San Luis; Argentina
Fil: Cortiñas, Teresa Inés. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - San Luis. Instituto Multidisciplinario de Investigaciones Biológicas de San Luis. Universidad Nacional de San Luis. Facultad de Ciencias Físico Matemáticas y Naturales. Instituto Multidisciplinario de Investigaciones Biológicas de San Luis; Argentina
Fil: Stagnitta, Patricia Virginia. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - San Luis. Instituto Multidisciplinario de Investigaciones Biológicas de San Luis. Universidad Nacional de San Luis. Facultad de Ciencias Físico Matemáticas y Naturales. Instituto Multidisciplinario de Investigaciones Biológicas de San Luis; Argentina
Materia
Biofilm
C. perfringens
Sanitizers
Glucose,
Morphology
CcpA
cpe
Nivel de accesibilidad
acceso abierto
Condiciones de uso
https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
Repositorio
CONICET Digital (CONICET)
Institución
Consejo Nacional de Investigaciones Científicas y Técnicas
OAI Identificador
oai:ri.conicet.gov.ar:11336/64652
Acceder
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oai_identifier_str oai:ri.conicet.gov.ar:11336/64652
network_acronym_str CONICETDig
repository_id_str 3498
network_name_str CONICET Digital (CONICET)
spelling Effect of Sanitizers and Glucose on Clostridium perfringens Biofilm Formation and GrowthCastro, Melina GabrielaIcazatti Zuñiga, Alejandro ArielDivizia, Maria JuliaVega, Alba EdithCortiñas, Teresa InésStagnitta, Patricia VirginiaBiofilmC. perfringensSanitizersGlucose,MorphologyCcpAcpehttps://purl.org/becyt/ford/1.6https://purl.org/becyt/ford/1Clostridium perfringens enterotoxigenic strain is one of the responsible of food poisoning; this is caused by cpe enterotoxin. Cpe is produced during sporulation, a resistance mechanism. It is described that C. perfringens can form biofilm under stressed environments and this depends of carbohydrates availability. The aim of this work was to evaluate the ability of enteroxigenic (E+) and no enterotoxigenic (E-) C. perfringens strains to form biofilm at different concentrations of carbon source and the inhibitory effect of various Poli P over biofilm formation and the expression of cpe and ccpa genes. Our results indicate that E+ C. perfringens biofilm reach the more complex structure with high glucose concentrations. Even more current sanitizers used in food industry like SAPP and TSPP, showed to stimulate biofilm formation and to inhibit the growth of planktonic cells. On the other hand, morphological changes were observed during biofilm formation under low glucose concentration and in presence of sanitizers. We propose that the change from a bacillary to coccoid form is related to a resistance to stressed environments. Finally the cpe expression is not related to biofilm formation.Fil: Castro, Melina Gabriela. Universidad Nacional de San Luis; ArgentinaFil: Icazatti Zuñiga, Alejandro Ariel. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - San Luis. Instituto de Matemática Aplicada de San Luis ; ArgentinaFil: Divizia, Maria Julia. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - San Luis. Instituto Multidisciplinario de Investigaciones Biológicas de San Luis. Universidad Nacional de San Luis. Facultad de Ciencias Físico Matemáticas y Naturales. Instituto Multidisciplinario de Investigaciones Biológicas de San Luis; ArgentinaFil: Vega, Alba Edith. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - San Luis. Instituto Multidisciplinario de Investigaciones Biológicas de San Luis. Universidad Nacional de San Luis. Facultad de Ciencias Físico Matemáticas y Naturales. Instituto Multidisciplinario de Investigaciones Biológicas de San Luis; ArgentinaFil: Cortiñas, Teresa Inés. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - San Luis. Instituto Multidisciplinario de Investigaciones Biológicas de San Luis. Universidad Nacional de San Luis. Facultad de Ciencias Físico Matemáticas y Naturales. Instituto Multidisciplinario de Investigaciones Biológicas de San Luis; ArgentinaFil: Stagnitta, Patricia Virginia. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - San Luis. Instituto Multidisciplinario de Investigaciones Biológicas de San Luis. Universidad Nacional de San Luis. Facultad de Ciencias Físico Matemáticas y Naturales. Instituto Multidisciplinario de Investigaciones Biológicas de San Luis; ArgentinaExcellent Publishers2016-07info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionhttp://purl.org/coar/resource_type/c_6501info:ar-repo/semantics/articuloapplication/pdfapplication/pdfhttp://hdl.handle.net/11336/64652Castro, Melina Gabriela; Icazatti Zuñiga, Alejandro Ariel; Divizia, Maria Julia; Vega, Alba Edith; Cortiñas, Teresa Inés; et al.; Effect of Sanitizers and Glucose on Clostridium perfringens Biofilm Formation and Growth; Excellent Publishers; International Journal of Current Microbiology and Applied Sciences; 5; 7; 7-2016; 210-2232319-7706CONICET DigitalCONICETenginfo:eu-repo/semantics/altIdentifier/doi/10.20546/ijcmas.2016.507.022info:eu-repo/semantics/altIdentifier/url/https://www.ijcmas.com/abstractview.php?ID=649&vol=5-7-2016&SNo=22info:eu-repo/semantics/openAccesshttps://creativecommons.org/licenses/by-nc-sa/2.5/ar/reponame:CONICET Digital (CONICET)instname:Consejo Nacional de Investigaciones Científicas y Técnicas2025-09-29T10:01:13Zoai:ri.conicet.gov.ar:11336/64652instacron:CONICETInstitucionalhttp://ri.conicet.gov.ar/Organismo científico-tecnológicoNo correspondehttp://ri.conicet.gov.ar/oai/requestdasensio@conicet.gov.ar; lcarlino@conicet.gov.arArgentinaNo correspondeNo correspondeNo correspondeopendoar:34982025-09-29 10:01:13.453CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicasfalse
dc.title.none.fl_str_mv Effect of Sanitizers and Glucose on Clostridium perfringens Biofilm Formation and Growth
title Effect of Sanitizers and Glucose on Clostridium perfringens Biofilm Formation and Growth
spellingShingle Effect of Sanitizers and Glucose on Clostridium perfringens Biofilm Formation and Growth
Castro, Melina Gabriela
Biofilm
C. perfringens
Sanitizers
Glucose,
Morphology
CcpA
cpe
title_short Effect of Sanitizers and Glucose on Clostridium perfringens Biofilm Formation and Growth
title_full Effect of Sanitizers and Glucose on Clostridium perfringens Biofilm Formation and Growth
title_fullStr Effect of Sanitizers and Glucose on Clostridium perfringens Biofilm Formation and Growth
title_full_unstemmed Effect of Sanitizers and Glucose on Clostridium perfringens Biofilm Formation and Growth
title_sort Effect of Sanitizers and Glucose on Clostridium perfringens Biofilm Formation and Growth
dc.creator.none.fl_str_mv Castro, Melina Gabriela
Icazatti Zuñiga, Alejandro Ariel
Divizia, Maria Julia
Vega, Alba Edith
Cortiñas, Teresa Inés
Stagnitta, Patricia Virginia
author Castro, Melina Gabriela
author_facet Castro, Melina Gabriela
Icazatti Zuñiga, Alejandro Ariel
Divizia, Maria Julia
Vega, Alba Edith
Cortiñas, Teresa Inés
Stagnitta, Patricia Virginia
author_role author
author2 Icazatti Zuñiga, Alejandro Ariel
Divizia, Maria Julia
Vega, Alba Edith
Cortiñas, Teresa Inés
Stagnitta, Patricia Virginia
author2_role author
author
author
author
author
dc.subject.none.fl_str_mv Biofilm
C. perfringens
Sanitizers
Glucose,
Morphology
CcpA
cpe
topic Biofilm
C. perfringens
Sanitizers
Glucose,
Morphology
CcpA
cpe
purl_subject.fl_str_mv https://purl.org/becyt/ford/1.6
https://purl.org/becyt/ford/1
dc.description.none.fl_txt_mv Clostridium perfringens enterotoxigenic strain is one of the responsible of food poisoning; this is caused by cpe enterotoxin. Cpe is produced during sporulation, a resistance mechanism. It is described that C. perfringens can form biofilm under stressed environments and this depends of carbohydrates availability. The aim of this work was to evaluate the ability of enteroxigenic (E+) and no enterotoxigenic (E-) C. perfringens strains to form biofilm at different concentrations of carbon source and the inhibitory effect of various Poli P over biofilm formation and the expression of cpe and ccpa genes. Our results indicate that E+ C. perfringens biofilm reach the more complex structure with high glucose concentrations. Even more current sanitizers used in food industry like SAPP and TSPP, showed to stimulate biofilm formation and to inhibit the growth of planktonic cells. On the other hand, morphological changes were observed during biofilm formation under low glucose concentration and in presence of sanitizers. We propose that the change from a bacillary to coccoid form is related to a resistance to stressed environments. Finally the cpe expression is not related to biofilm formation.
Fil: Castro, Melina Gabriela. Universidad Nacional de San Luis; Argentina
Fil: Icazatti Zuñiga, Alejandro Ariel. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - San Luis. Instituto de Matemática Aplicada de San Luis ; Argentina
Fil: Divizia, Maria Julia. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - San Luis. Instituto Multidisciplinario de Investigaciones Biológicas de San Luis. Universidad Nacional de San Luis. Facultad de Ciencias Físico Matemáticas y Naturales. Instituto Multidisciplinario de Investigaciones Biológicas de San Luis; Argentina
Fil: Vega, Alba Edith. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - San Luis. Instituto Multidisciplinario de Investigaciones Biológicas de San Luis. Universidad Nacional de San Luis. Facultad de Ciencias Físico Matemáticas y Naturales. Instituto Multidisciplinario de Investigaciones Biológicas de San Luis; Argentina
Fil: Cortiñas, Teresa Inés. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - San Luis. Instituto Multidisciplinario de Investigaciones Biológicas de San Luis. Universidad Nacional de San Luis. Facultad de Ciencias Físico Matemáticas y Naturales. Instituto Multidisciplinario de Investigaciones Biológicas de San Luis; Argentina
Fil: Stagnitta, Patricia Virginia. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - San Luis. Instituto Multidisciplinario de Investigaciones Biológicas de San Luis. Universidad Nacional de San Luis. Facultad de Ciencias Físico Matemáticas y Naturales. Instituto Multidisciplinario de Investigaciones Biológicas de San Luis; Argentina
description Clostridium perfringens enterotoxigenic strain is one of the responsible of food poisoning; this is caused by cpe enterotoxin. Cpe is produced during sporulation, a resistance mechanism. It is described that C. perfringens can form biofilm under stressed environments and this depends of carbohydrates availability. The aim of this work was to evaluate the ability of enteroxigenic (E+) and no enterotoxigenic (E-) C. perfringens strains to form biofilm at different concentrations of carbon source and the inhibitory effect of various Poli P over biofilm formation and the expression of cpe and ccpa genes. Our results indicate that E+ C. perfringens biofilm reach the more complex structure with high glucose concentrations. Even more current sanitizers used in food industry like SAPP and TSPP, showed to stimulate biofilm formation and to inhibit the growth of planktonic cells. On the other hand, morphological changes were observed during biofilm formation under low glucose concentration and in presence of sanitizers. We propose that the change from a bacillary to coccoid form is related to a resistance to stressed environments. Finally the cpe expression is not related to biofilm formation.
publishDate 2016
dc.date.none.fl_str_mv 2016-07
dc.type.none.fl_str_mv info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
http://purl.org/coar/resource_type/c_6501
info:ar-repo/semantics/articulo
format article
status_str publishedVersion
dc.identifier.none.fl_str_mv http://hdl.handle.net/11336/64652
Castro, Melina Gabriela; Icazatti Zuñiga, Alejandro Ariel; Divizia, Maria Julia; Vega, Alba Edith; Cortiñas, Teresa Inés; et al.; Effect of Sanitizers and Glucose on Clostridium perfringens Biofilm Formation and Growth; Excellent Publishers; International Journal of Current Microbiology and Applied Sciences; 5; 7; 7-2016; 210-223
2319-7706
CONICET Digital
CONICET
url http://hdl.handle.net/11336/64652
identifier_str_mv Castro, Melina Gabriela; Icazatti Zuñiga, Alejandro Ariel; Divizia, Maria Julia; Vega, Alba Edith; Cortiñas, Teresa Inés; et al.; Effect of Sanitizers and Glucose on Clostridium perfringens Biofilm Formation and Growth; Excellent Publishers; International Journal of Current Microbiology and Applied Sciences; 5; 7; 7-2016; 210-223
2319-7706
CONICET Digital
CONICET
dc.language.none.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv info:eu-repo/semantics/altIdentifier/doi/10.20546/ijcmas.2016.507.022
info:eu-repo/semantics/altIdentifier/url/https://www.ijcmas.com/abstractview.php?ID=649&vol=5-7-2016&SNo=22
dc.rights.none.fl_str_mv info:eu-repo/semantics/openAccess
https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
eu_rights_str_mv openAccess
rights_invalid_str_mv https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
dc.format.none.fl_str_mv application/pdf
application/pdf
dc.publisher.none.fl_str_mv Excellent Publishers
publisher.none.fl_str_mv Excellent Publishers
dc.source.none.fl_str_mv reponame:CONICET Digital (CONICET)
instname:Consejo Nacional de Investigaciones Científicas y Técnicas
reponame_str CONICET Digital (CONICET)
collection CONICET Digital (CONICET)
instname_str Consejo Nacional de Investigaciones Científicas y Técnicas
repository.name.fl_str_mv CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicas
repository.mail.fl_str_mv dasensio@conicet.gov.ar; lcarlino@conicet.gov.ar
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score 13.070432

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