Molecular and functional characterization of a Trypanosoma cruzi nuclear adenylate kinase isoform

• Autores: Camara, Maria de Los Milagros; Bouvier, Leon Alberto; Canepa, Gaspar Exequiel; Miranda, Mariana Reneé; Pereira, Claudio Alejandro
• Año de publicación: 2013
• Idioma: inglés
• Tipo de recurso: artículo
• Materia: Trypanosoma cruzi; Chagas disease; Adenylate kinase; Ribosome processing
• Institución: Consejo Nacional de Investigaciones Científicas y Técnicas

Trypanosoma cruzi, the etiological agent of Chagas’ disease, is an early divergent eukaryote in which control of gene expression relies mainly in post-transcriptional mechanisms. Transcription levels are globally up and down regulated during the transition between proliferating and non-proliferating life-cycle stages. In this work we characterized a nuclear adenylate kinase isoform (TcADKn) that is involved in ribosome biogenesis. Nuclear adenylate kinases have been recently described in a few organisms, being all related to RNA metabolism. Depending on active transcription and translation, TcADKn localizes in the nucleolus or the cytoplasm. A non-canonical nuclear localization signal was mapped towards the Nterminal of the protein, being the phosphate-binding loop essential for its localization. In addition, TcADKn nuclear exportation depends on the nuclear exportation adapter CRM1. TcADKn nuclear shuttling is governed by nutrient availability, oxidative stress and by the equivalent in T. cruzi of the mammalian TOR (Target of Rapamycin) pathway. One of the biological functions of TcADKn is ribosomal 18S RNA processing by direct interaction with ribosomal protein TcRps14. Finally, TcADKn expression is regulated by its 39 UTR mRNA. Depending on extracellular conditions, cells modulate protein translation rates regulating ribosome biogenesis and nuclear adenylate kinases are probably key components in these processes.
Fil: Camara, Maria de Los Milagros. Universidad de Buenos Aires. Facultad de Medicina. Instituto de Investigaciones Médicas "Dr. Alfredo Lanari"; Argentina;
Fil: Bouvier, Leon Alberto. Universidad de Buenos Aires. Facultad de Medicina. Instituto de Investigaciones Médicas "Dr. Alfredo Lanari"; Argentina;
Fil: Canepa, Gaspar Exequiel. Universidad de Buenos Aires. Facultad de Medicina. Instituto de Investigaciones Médicas "Dr. Alfredo Lanari"; Argentina;
Fil: Miranda, Mariana Reneé. Universidad de Buenos Aires. Facultad de Medicina. Instituto de Investigaciones Médicas "Dr. Alfredo Lanari"; Argentina;
Fil: Pereira, Claudio Alejandro. Universidad de Buenos Aires. Facultad de Medicina. Instituto de Investigaciones Médicas "Dr. Alfredo Lanari"; Argentina;