Pea3 transcription factor family members Etv4 Etv5 mediate retrograde signalling and axonal growth of DRG sensory neurons in response to NGF

Autores
Fontanet, Paula; Irala, Dolores; Alsina, Fernando Cruz; Paratcha, Gustavo; Ledda, Maria Fernanda
Año de publicación
2013
Idioma
inglés
Tipo de recurso
artículo
Estado
versión publicada
Descripción
Nerve growth factor (NGF) is a target-derived neurotrophic growth factor that controls many aspects of sensory and sympathetic neuronal development. The identification of transcription factors and downstream target genes that mediate NGF-dependent neuronal differentiation and target field innervation is currently a major challenge. Here, we show that the Pea3 transcription factor family members Etv4 and Etv5 are expressed by developing TrkA-positive dorsal root ganglion (DRG) neurons during the period of target innervation. Real-time PCR assays indicated that Etv4 and Etv5 mRNAs are significantly induced by NGF in different neuronal cells, suggesting that they could be involved in the biological responses induced by this neurotrophin. Interestingly, distal axon application of NGF in compartmentalized cultures of rat DRG sensory neurons was sufficient to induce a significant increase in Etv4 and Etv5 mRNA expression. Pharmacological assays also revealed that activation of MEK/ERK (MAPK) pathway is required for Etv4 and Etv5 gene induction in response to NGF. Downregulation of Etv4 and Etv5 using small interference RNA knockdown experiments inhibited NGF-induced neurite outgrowth of rat sensory neurons, while overexpression of full-length Etv4 or Etv5 potentiated neuronal differentiation in response to this neurotrophin. Together, these data establish Etv4 and Etv5 as essential molecules of the transcriptional program linking neurotrophin signaling to sensory neuronal differentiation, and suggest that they can be involved in NGF-mediated target innervation.
Fil: Fontanet, Paula. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Houssay. Instituto de Biología Celular y Neurociencias "profesor Eduardo de Robertis"; Argentina
Fil: Irala, Dolores. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Houssay. Instituto de Biología Celular y Neurociencias "profesor Eduardo de Robertis"; Argentina
Fil: Alsina, Fernando Cruz. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Houssay. Instituto de Biología Celular y Neurociencias "profesor Eduardo de Robertis"; Argentina
Fil: Paratcha, Gustavo. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Houssay. Instituto de Biología Celular y Neurociencias "profesor Eduardo de Robertis"; Argentina. Karolinska Huddinge Hospital. Karolinska Institutet; Suecia
Fil: Ledda, Maria Fernanda. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Houssay. Instituto de Biología Celular y Neurociencias "profesor Eduardo de Robertis"; Argentina. Karolinska Huddinge Hospital. Karolinska Institutet; Suecia
Materia
NGF
Dorsal root Ganglia
Signaling
Transcription factors
Nivel de accesibilidad
acceso abierto
Condiciones de uso
https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
Repositorio
CONICET Digital (CONICET)
Institución
Consejo Nacional de Investigaciones Científicas y Técnicas
OAI Identificador
oai:ri.conicet.gov.ar:11336/8385
Acceder
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oai_identifier_str oai:ri.conicet.gov.ar:11336/8385
network_acronym_str CONICETDig
repository_id_str 3498
network_name_str CONICET Digital (CONICET)
spelling Pea3 transcription factor family members Etv4 Etv5 mediate retrograde signalling and axonal growth of DRG sensory neurons in response to NGFFontanet, PaulaIrala, DoloresAlsina, Fernando CruzParatcha, GustavoLedda, Maria FernandaNGFDorsal root GangliaSignalingTranscription factorshttps://purl.org/becyt/ford/1.6https://purl.org/becyt/ford/1Nerve growth factor (NGF) is a target-derived neurotrophic growth factor that controls many aspects of sensory and sympathetic neuronal development. The identification of transcription factors and downstream target genes that mediate NGF-dependent neuronal differentiation and target field innervation is currently a major challenge. Here, we show that the Pea3 transcription factor family members Etv4 and Etv5 are expressed by developing TrkA-positive dorsal root ganglion (DRG) neurons during the period of target innervation. Real-time PCR assays indicated that Etv4 and Etv5 mRNAs are significantly induced by NGF in different neuronal cells, suggesting that they could be involved in the biological responses induced by this neurotrophin. Interestingly, distal axon application of NGF in compartmentalized cultures of rat DRG sensory neurons was sufficient to induce a significant increase in Etv4 and Etv5 mRNA expression. Pharmacological assays also revealed that activation of MEK/ERK (MAPK) pathway is required for Etv4 and Etv5 gene induction in response to NGF. Downregulation of Etv4 and Etv5 using small interference RNA knockdown experiments inhibited NGF-induced neurite outgrowth of rat sensory neurons, while overexpression of full-length Etv4 or Etv5 potentiated neuronal differentiation in response to this neurotrophin. Together, these data establish Etv4 and Etv5 as essential molecules of the transcriptional program linking neurotrophin signaling to sensory neuronal differentiation, and suggest that they can be involved in NGF-mediated target innervation.Fil: Fontanet, Paula. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Houssay. Instituto de Biología Celular y Neurociencias "profesor Eduardo de Robertis"; ArgentinaFil: Irala, Dolores. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Houssay. Instituto de Biología Celular y Neurociencias "profesor Eduardo de Robertis"; ArgentinaFil: Alsina, Fernando Cruz. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Houssay. Instituto de Biología Celular y Neurociencias "profesor Eduardo de Robertis"; ArgentinaFil: Paratcha, Gustavo. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Houssay. Instituto de Biología Celular y Neurociencias "profesor Eduardo de Robertis"; Argentina. Karolinska Huddinge Hospital. Karolinska Institutet; SueciaFil: Ledda, Maria Fernanda. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Houssay. Instituto de Biología Celular y Neurociencias "profesor Eduardo de Robertis"; Argentina. Karolinska Huddinge Hospital. Karolinska Institutet; SueciaSociety For Neuroscience2013-10info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionhttp://purl.org/coar/resource_type/c_6501info:ar-repo/semantics/articuloapplication/pdfapplication/pdfhttp://hdl.handle.net/11336/8385Fontanet, Paula; Irala, Dolores; Alsina, Fernando Cruz; Paratcha, Gustavo; Ledda, Maria Fernanda; Pea3 transcription factor family members Etv4 Etv5 mediate retrograde signalling and axonal growth of DRG sensory neurons in response to NGF; Society For Neuroscience; Journal Of Neuroscience; 33; 40; 10-2013; 15940-159510270-6474enginfo:eu-repo/semantics/altIdentifier/url/http://www.jneurosci.org/content/33/40/15940info:eu-repo/semantics/altIdentifier/doi/10.1523/JNEUROSCI.0928-13.2013info:eu-repo/semantics/openAccesshttps://creativecommons.org/licenses/by-nc-sa/2.5/ar/reponame:CONICET Digital (CONICET)instname:Consejo Nacional de Investigaciones Científicas y Técnicas2025-10-15T15:08:13Zoai:ri.conicet.gov.ar:11336/8385instacron:CONICETInstitucionalhttp://ri.conicet.gov.ar/Organismo científico-tecnológicoNo correspondehttp://ri.conicet.gov.ar/oai/requestdasensio@conicet.gov.ar; lcarlino@conicet.gov.arArgentinaNo correspondeNo correspondeNo correspondeopendoar:34982025-10-15 15:08:13.313CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicasfalse
dc.title.none.fl_str_mv Pea3 transcription factor family members Etv4 Etv5 mediate retrograde signalling and axonal growth of DRG sensory neurons in response to NGF
title Pea3 transcription factor family members Etv4 Etv5 mediate retrograde signalling and axonal growth of DRG sensory neurons in response to NGF
spellingShingle Pea3 transcription factor family members Etv4 Etv5 mediate retrograde signalling and axonal growth of DRG sensory neurons in response to NGF
Fontanet, Paula
NGF
Dorsal root Ganglia
Signaling
Transcription factors
title_short Pea3 transcription factor family members Etv4 Etv5 mediate retrograde signalling and axonal growth of DRG sensory neurons in response to NGF
title_full Pea3 transcription factor family members Etv4 Etv5 mediate retrograde signalling and axonal growth of DRG sensory neurons in response to NGF
title_fullStr Pea3 transcription factor family members Etv4 Etv5 mediate retrograde signalling and axonal growth of DRG sensory neurons in response to NGF
title_full_unstemmed Pea3 transcription factor family members Etv4 Etv5 mediate retrograde signalling and axonal growth of DRG sensory neurons in response to NGF
title_sort Pea3 transcription factor family members Etv4 Etv5 mediate retrograde signalling and axonal growth of DRG sensory neurons in response to NGF
dc.creator.none.fl_str_mv Fontanet, Paula
Irala, Dolores
Alsina, Fernando Cruz
Paratcha, Gustavo
Ledda, Maria Fernanda
author Fontanet, Paula
author_facet Fontanet, Paula
Irala, Dolores
Alsina, Fernando Cruz
Paratcha, Gustavo
Ledda, Maria Fernanda
author_role author
author2 Irala, Dolores
Alsina, Fernando Cruz
Paratcha, Gustavo
Ledda, Maria Fernanda
author2_role author
author
author
author
dc.subject.none.fl_str_mv NGF
Dorsal root Ganglia
Signaling
Transcription factors
topic NGF
Dorsal root Ganglia
Signaling
Transcription factors
purl_subject.fl_str_mv https://purl.org/becyt/ford/1.6
https://purl.org/becyt/ford/1
dc.description.none.fl_txt_mv Nerve growth factor (NGF) is a target-derived neurotrophic growth factor that controls many aspects of sensory and sympathetic neuronal development. The identification of transcription factors and downstream target genes that mediate NGF-dependent neuronal differentiation and target field innervation is currently a major challenge. Here, we show that the Pea3 transcription factor family members Etv4 and Etv5 are expressed by developing TrkA-positive dorsal root ganglion (DRG) neurons during the period of target innervation. Real-time PCR assays indicated that Etv4 and Etv5 mRNAs are significantly induced by NGF in different neuronal cells, suggesting that they could be involved in the biological responses induced by this neurotrophin. Interestingly, distal axon application of NGF in compartmentalized cultures of rat DRG sensory neurons was sufficient to induce a significant increase in Etv4 and Etv5 mRNA expression. Pharmacological assays also revealed that activation of MEK/ERK (MAPK) pathway is required for Etv4 and Etv5 gene induction in response to NGF. Downregulation of Etv4 and Etv5 using small interference RNA knockdown experiments inhibited NGF-induced neurite outgrowth of rat sensory neurons, while overexpression of full-length Etv4 or Etv5 potentiated neuronal differentiation in response to this neurotrophin. Together, these data establish Etv4 and Etv5 as essential molecules of the transcriptional program linking neurotrophin signaling to sensory neuronal differentiation, and suggest that they can be involved in NGF-mediated target innervation.
Fil: Fontanet, Paula. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Houssay. Instituto de Biología Celular y Neurociencias "profesor Eduardo de Robertis"; Argentina
Fil: Irala, Dolores. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Houssay. Instituto de Biología Celular y Neurociencias "profesor Eduardo de Robertis"; Argentina
Fil: Alsina, Fernando Cruz. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Houssay. Instituto de Biología Celular y Neurociencias "profesor Eduardo de Robertis"; Argentina
Fil: Paratcha, Gustavo. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Houssay. Instituto de Biología Celular y Neurociencias "profesor Eduardo de Robertis"; Argentina. Karolinska Huddinge Hospital. Karolinska Institutet; Suecia
Fil: Ledda, Maria Fernanda. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Houssay. Instituto de Biología Celular y Neurociencias "profesor Eduardo de Robertis"; Argentina. Karolinska Huddinge Hospital. Karolinska Institutet; Suecia
description Nerve growth factor (NGF) is a target-derived neurotrophic growth factor that controls many aspects of sensory and sympathetic neuronal development. The identification of transcription factors and downstream target genes that mediate NGF-dependent neuronal differentiation and target field innervation is currently a major challenge. Here, we show that the Pea3 transcription factor family members Etv4 and Etv5 are expressed by developing TrkA-positive dorsal root ganglion (DRG) neurons during the period of target innervation. Real-time PCR assays indicated that Etv4 and Etv5 mRNAs are significantly induced by NGF in different neuronal cells, suggesting that they could be involved in the biological responses induced by this neurotrophin. Interestingly, distal axon application of NGF in compartmentalized cultures of rat DRG sensory neurons was sufficient to induce a significant increase in Etv4 and Etv5 mRNA expression. Pharmacological assays also revealed that activation of MEK/ERK (MAPK) pathway is required for Etv4 and Etv5 gene induction in response to NGF. Downregulation of Etv4 and Etv5 using small interference RNA knockdown experiments inhibited NGF-induced neurite outgrowth of rat sensory neurons, while overexpression of full-length Etv4 or Etv5 potentiated neuronal differentiation in response to this neurotrophin. Together, these data establish Etv4 and Etv5 as essential molecules of the transcriptional program linking neurotrophin signaling to sensory neuronal differentiation, and suggest that they can be involved in NGF-mediated target innervation.
publishDate 2013
dc.date.none.fl_str_mv 2013-10
dc.type.none.fl_str_mv info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
http://purl.org/coar/resource_type/c_6501
info:ar-repo/semantics/articulo
format article
status_str publishedVersion
dc.identifier.none.fl_str_mv http://hdl.handle.net/11336/8385
Fontanet, Paula; Irala, Dolores; Alsina, Fernando Cruz; Paratcha, Gustavo; Ledda, Maria Fernanda; Pea3 transcription factor family members Etv4 Etv5 mediate retrograde signalling and axonal growth of DRG sensory neurons in response to NGF; Society For Neuroscience; Journal Of Neuroscience; 33; 40; 10-2013; 15940-15951
0270-6474
url http://hdl.handle.net/11336/8385
identifier_str_mv Fontanet, Paula; Irala, Dolores; Alsina, Fernando Cruz; Paratcha, Gustavo; Ledda, Maria Fernanda; Pea3 transcription factor family members Etv4 Etv5 mediate retrograde signalling and axonal growth of DRG sensory neurons in response to NGF; Society For Neuroscience; Journal Of Neuroscience; 33; 40; 10-2013; 15940-15951
0270-6474
dc.language.none.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv info:eu-repo/semantics/altIdentifier/url/http://www.jneurosci.org/content/33/40/15940
info:eu-repo/semantics/altIdentifier/doi/10.1523/JNEUROSCI.0928-13.2013
dc.rights.none.fl_str_mv info:eu-repo/semantics/openAccess
https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
eu_rights_str_mv openAccess
rights_invalid_str_mv https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
dc.format.none.fl_str_mv application/pdf
application/pdf
dc.publisher.none.fl_str_mv Society For Neuroscience
publisher.none.fl_str_mv Society For Neuroscience
dc.source.none.fl_str_mv reponame:CONICET Digital (CONICET)
instname:Consejo Nacional de Investigaciones Científicas y Técnicas
reponame_str CONICET Digital (CONICET)
collection CONICET Digital (CONICET)
instname_str Consejo Nacional de Investigaciones Científicas y Técnicas
repository.name.fl_str_mv CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicas
repository.mail.fl_str_mv dasensio@conicet.gov.ar; lcarlino@conicet.gov.ar
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score 13.22299

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