Protein malnutrition impairs the immune control of Trichinella spiralis infection

Autores
Vila, Cecilia Celeste; Saracino, María Priscila; Falduto, Guido Hernán; Calcagno, Marcela Adriana; Venturiello, Stella Maris; Pallaro, Anabel Nora; Baldi, Pablo Cesar
Año de publicación
2019
Idioma
inglés
Tipo de recurso
artículo
Estado
versión publicada
Descripción
Objectives: We aimed to analyze the effect of a protein-deficient diet on mucosal and systemic immunity during a Trichinella spiralis infection. Methods: Two groups of weaning Wistar rats received a protein-deficient diet (6.5% casein) and the other two groups received a control diet (20% casein). After 10 d, one group of each diet was infected (PD I and C I ) with muscle larvae (infecting stage). Food intake and body weight were assessed over time. Blood eosinophils counts, antibodies in serum, and tissue extracts were assessed at different days postinfection. Histologic studies were done in the lungs and intestines, and adult worm (AW) fecundity index score and muscle parasite burden were determined. Results: Food and protein intake were lower in PD I than in C I. Body weight was lower in PD I than in a non-infected protein-deficient diet. Eosinophils counts were lower in PD I than in C I. Total and specific antibodies were lower in PD I than C I. PD I had a reduced number of mast and goblet cells in the lungs and intestines compared with C I. The persistence of AW in the intestines and migrant larvae at the lungs was longer in PD I than in C I. . The AW fecundity index score was higher in PD I than in C I . Finally, PD I evidenced a higher muscular parasite burden than C I . Conclusions: Protein deficiency affects the mucosal and systemic immune response to Trichinella spiralis and delays the expulsion and increases the fecundity index score of AW, which leads to a higher parasite burden in the muscles.
Fil: Vila, Cecilia Celeste. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Houssay. Instituto de Estudios de la Inmunidad Humoral Prof. Ricardo A. Margni. Universidad de Buenos Aires. Facultad de Farmacia y Bioquímica. Instituto de Estudios de la Inmunidad Humoral Prof. Ricardo A. Margni; Argentina
Fil: Saracino, María Priscila. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Houssay. Instituto de Estudios de la Inmunidad Humoral Prof. Ricardo A. Margni. Universidad de Buenos Aires. Facultad de Farmacia y Bioquímica. Instituto de Estudios de la Inmunidad Humoral Prof. Ricardo A. Margni; Argentina
Fil: Falduto, Guido Hernán. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Houssay. Instituto de Estudios de la Inmunidad Humoral Prof. Ricardo A. Margni. Universidad de Buenos Aires. Facultad de Farmacia y Bioquímica. Instituto de Estudios de la Inmunidad Humoral Prof. Ricardo A. Margni; Argentina
Fil: Calcagno, Marcela Adriana. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Houssay. Instituto de Estudios de la Inmunidad Humoral Prof. Ricardo A. Margni. Universidad de Buenos Aires. Facultad de Farmacia y Bioquímica. Instituto de Estudios de la Inmunidad Humoral Prof. Ricardo A. Margni; Argentina
Fil: Venturiello, Stella Maris. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Houssay. Instituto de Estudios de la Inmunidad Humoral Prof. Ricardo A. Margni. Universidad de Buenos Aires. Facultad de Farmacia y Bioquímica. Instituto de Estudios de la Inmunidad Humoral Prof. Ricardo A. Margni; Argentina
Fil: Pallaro, Anabel Nora. Universidad de Buenos Aires. Facultad de Farmacia y Bioquímica. Departamento de Bromatología y Nutrición Experimental; Argentina
Fil: Baldi, Pablo Cesar. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Houssay. Instituto de Estudios de la Inmunidad Humoral Prof. Ricardo A. Margni. Universidad de Buenos Aires. Facultad de Farmacia y Bioquímica. Instituto de Estudios de la Inmunidad Humoral Prof. Ricardo A. Margni; Argentina
Materia
IMMUNOLOGICAL PARAMETERS
NUTRITIONAL PARAMETERS
PARASITOLOGICAL PARAMETERS
PROTEIN DEFICIENCY
TRICHINELLA SPIRALIS INFECTION
Nivel de accesibilidad
acceso abierto
Condiciones de uso
https://creativecommons.org/licenses/by-nc-nd/2.5/ar/
Repositorio
CONICET Digital (CONICET)
Institución
Consejo Nacional de Investigaciones Científicas y Técnicas
OAI Identificador
oai:ri.conicet.gov.ar:11336/130329
Acceder
id CONICETDig_17629a9fec78ae150e7e3fa6eabb0283
oai_identifier_str oai:ri.conicet.gov.ar:11336/130329
network_acronym_str CONICETDig
repository_id_str 3498
network_name_str CONICET Digital (CONICET)
spelling Protein malnutrition impairs the immune control of Trichinella spiralis infectionVila, Cecilia CelesteSaracino, María PriscilaFalduto, Guido HernánCalcagno, Marcela AdrianaVenturiello, Stella MarisPallaro, Anabel NoraBaldi, Pablo CesarIMMUNOLOGICAL PARAMETERSNUTRITIONAL PARAMETERSPARASITOLOGICAL PARAMETERSPROTEIN DEFICIENCYTRICHINELLA SPIRALIS INFECTIONhttps://purl.org/becyt/ford/3.1https://purl.org/becyt/ford/3https://purl.org/becyt/ford/3.3https://purl.org/becyt/ford/3Objectives: We aimed to analyze the effect of a protein-deficient diet on mucosal and systemic immunity during a Trichinella spiralis infection. Methods: Two groups of weaning Wistar rats received a protein-deficient diet (6.5% casein) and the other two groups received a control diet (20% casein). After 10 d, one group of each diet was infected (PD I and C I ) with muscle larvae (infecting stage). Food intake and body weight were assessed over time. Blood eosinophils counts, antibodies in serum, and tissue extracts were assessed at different days postinfection. Histologic studies were done in the lungs and intestines, and adult worm (AW) fecundity index score and muscle parasite burden were determined. Results: Food and protein intake were lower in PD I than in C I. Body weight was lower in PD I than in a non-infected protein-deficient diet. Eosinophils counts were lower in PD I than in C I. Total and specific antibodies were lower in PD I than C I. PD I had a reduced number of mast and goblet cells in the lungs and intestines compared with C I. The persistence of AW in the intestines and migrant larvae at the lungs was longer in PD I than in C I. . The AW fecundity index score was higher in PD I than in C I . Finally, PD I evidenced a higher muscular parasite burden than C I . Conclusions: Protein deficiency affects the mucosal and systemic immune response to Trichinella spiralis and delays the expulsion and increases the fecundity index score of AW, which leads to a higher parasite burden in the muscles.Fil: Vila, Cecilia Celeste. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Houssay. Instituto de Estudios de la Inmunidad Humoral Prof. Ricardo A. Margni. Universidad de Buenos Aires. Facultad de Farmacia y Bioquímica. Instituto de Estudios de la Inmunidad Humoral Prof. Ricardo A. Margni; ArgentinaFil: Saracino, María Priscila. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Houssay. Instituto de Estudios de la Inmunidad Humoral Prof. Ricardo A. Margni. Universidad de Buenos Aires. Facultad de Farmacia y Bioquímica. Instituto de Estudios de la Inmunidad Humoral Prof. Ricardo A. Margni; ArgentinaFil: Falduto, Guido Hernán. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Houssay. Instituto de Estudios de la Inmunidad Humoral Prof. Ricardo A. Margni. Universidad de Buenos Aires. Facultad de Farmacia y Bioquímica. Instituto de Estudios de la Inmunidad Humoral Prof. Ricardo A. Margni; ArgentinaFil: Calcagno, Marcela Adriana. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Houssay. Instituto de Estudios de la Inmunidad Humoral Prof. Ricardo A. Margni. Universidad de Buenos Aires. Facultad de Farmacia y Bioquímica. Instituto de Estudios de la Inmunidad Humoral Prof. Ricardo A. Margni; ArgentinaFil: Venturiello, Stella Maris. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Houssay. Instituto de Estudios de la Inmunidad Humoral Prof. Ricardo A. Margni. Universidad de Buenos Aires. Facultad de Farmacia y Bioquímica. Instituto de Estudios de la Inmunidad Humoral Prof. Ricardo A. Margni; ArgentinaFil: Pallaro, Anabel Nora. Universidad de Buenos Aires. Facultad de Farmacia y Bioquímica. Departamento de Bromatología y Nutrición Experimental; ArgentinaFil: Baldi, Pablo Cesar. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Houssay. Instituto de Estudios de la Inmunidad Humoral Prof. Ricardo A. Margni. Universidad de Buenos Aires. Facultad de Farmacia y Bioquímica. Instituto de Estudios de la Inmunidad Humoral Prof. Ricardo A. Margni; ArgentinaElsevier Science Inc2019-04info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionhttp://purl.org/coar/resource_type/c_6501info:ar-repo/semantics/articuloapplication/pdfapplication/pdfapplication/pdfapplication/pdfhttp://hdl.handle.net/11336/130329Vila, Cecilia Celeste; Saracino, María Priscila; Falduto, Guido Hernán; Calcagno, Marcela Adriana; Venturiello, Stella Maris; et al.; Protein malnutrition impairs the immune control of Trichinella spiralis infection; Elsevier Science Inc; Nutrition; 60; 4-2019; 161-1690899-9007CONICET DigitalCONICETenginfo:eu-repo/semantics/altIdentifier/url/https://www.sciencedirect.com/science/article/abs/pii/S0899900718308955info:eu-repo/semantics/altIdentifier/doi/10.1016/j.nut.2018.10.024info:eu-repo/semantics/openAccesshttps://creativecommons.org/licenses/by-nc-nd/2.5/ar/reponame:CONICET Digital (CONICET)instname:Consejo Nacional de Investigaciones Científicas y Técnicas2025-09-17T10:52:34Zoai:ri.conicet.gov.ar:11336/130329instacron:CONICETInstitucionalhttp://ri.conicet.gov.ar/Organismo científico-tecnológicoNo correspondehttp://ri.conicet.gov.ar/oai/requestdasensio@conicet.gov.ar; lcarlino@conicet.gov.arArgentinaNo correspondeNo correspondeNo correspondeopendoar:34982025-09-17 10:52:34.403CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicasfalse
dc.title.none.fl_str_mv Protein malnutrition impairs the immune control of Trichinella spiralis infection
title Protein malnutrition impairs the immune control of Trichinella spiralis infection
spellingShingle Protein malnutrition impairs the immune control of Trichinella spiralis infection
Vila, Cecilia Celeste
IMMUNOLOGICAL PARAMETERS
NUTRITIONAL PARAMETERS
PARASITOLOGICAL PARAMETERS
PROTEIN DEFICIENCY
TRICHINELLA SPIRALIS INFECTION
title_short Protein malnutrition impairs the immune control of Trichinella spiralis infection
title_full Protein malnutrition impairs the immune control of Trichinella spiralis infection
title_fullStr Protein malnutrition impairs the immune control of Trichinella spiralis infection
title_full_unstemmed Protein malnutrition impairs the immune control of Trichinella spiralis infection
title_sort Protein malnutrition impairs the immune control of Trichinella spiralis infection
dc.creator.none.fl_str_mv Vila, Cecilia Celeste
Saracino, María Priscila
Falduto, Guido Hernán
Calcagno, Marcela Adriana
Venturiello, Stella Maris
Pallaro, Anabel Nora
Baldi, Pablo Cesar
author Vila, Cecilia Celeste
author_facet Vila, Cecilia Celeste
Saracino, María Priscila
Falduto, Guido Hernán
Calcagno, Marcela Adriana
Venturiello, Stella Maris
Pallaro, Anabel Nora
Baldi, Pablo Cesar
author_role author
author2 Saracino, María Priscila
Falduto, Guido Hernán
Calcagno, Marcela Adriana
Venturiello, Stella Maris
Pallaro, Anabel Nora
Baldi, Pablo Cesar
author2_role author
author
author
author
author
author
dc.subject.none.fl_str_mv IMMUNOLOGICAL PARAMETERS
NUTRITIONAL PARAMETERS
PARASITOLOGICAL PARAMETERS
PROTEIN DEFICIENCY
TRICHINELLA SPIRALIS INFECTION
topic IMMUNOLOGICAL PARAMETERS
NUTRITIONAL PARAMETERS
PARASITOLOGICAL PARAMETERS
PROTEIN DEFICIENCY
TRICHINELLA SPIRALIS INFECTION
purl_subject.fl_str_mv https://purl.org/becyt/ford/3.1
https://purl.org/becyt/ford/3
https://purl.org/becyt/ford/3.3
https://purl.org/becyt/ford/3
dc.description.none.fl_txt_mv Objectives: We aimed to analyze the effect of a protein-deficient diet on mucosal and systemic immunity during a Trichinella spiralis infection. Methods: Two groups of weaning Wistar rats received a protein-deficient diet (6.5% casein) and the other two groups received a control diet (20% casein). After 10 d, one group of each diet was infected (PD I and C I ) with muscle larvae (infecting stage). Food intake and body weight were assessed over time. Blood eosinophils counts, antibodies in serum, and tissue extracts were assessed at different days postinfection. Histologic studies were done in the lungs and intestines, and adult worm (AW) fecundity index score and muscle parasite burden were determined. Results: Food and protein intake were lower in PD I than in C I. Body weight was lower in PD I than in a non-infected protein-deficient diet. Eosinophils counts were lower in PD I than in C I. Total and specific antibodies were lower in PD I than C I. PD I had a reduced number of mast and goblet cells in the lungs and intestines compared with C I. The persistence of AW in the intestines and migrant larvae at the lungs was longer in PD I than in C I. . The AW fecundity index score was higher in PD I than in C I . Finally, PD I evidenced a higher muscular parasite burden than C I . Conclusions: Protein deficiency affects the mucosal and systemic immune response to Trichinella spiralis and delays the expulsion and increases the fecundity index score of AW, which leads to a higher parasite burden in the muscles.
Fil: Vila, Cecilia Celeste. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Houssay. Instituto de Estudios de la Inmunidad Humoral Prof. Ricardo A. Margni. Universidad de Buenos Aires. Facultad de Farmacia y Bioquímica. Instituto de Estudios de la Inmunidad Humoral Prof. Ricardo A. Margni; Argentina
Fil: Saracino, María Priscila. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Houssay. Instituto de Estudios de la Inmunidad Humoral Prof. Ricardo A. Margni. Universidad de Buenos Aires. Facultad de Farmacia y Bioquímica. Instituto de Estudios de la Inmunidad Humoral Prof. Ricardo A. Margni; Argentina
Fil: Falduto, Guido Hernán. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Houssay. Instituto de Estudios de la Inmunidad Humoral Prof. Ricardo A. Margni. Universidad de Buenos Aires. Facultad de Farmacia y Bioquímica. Instituto de Estudios de la Inmunidad Humoral Prof. Ricardo A. Margni; Argentina
Fil: Calcagno, Marcela Adriana. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Houssay. Instituto de Estudios de la Inmunidad Humoral Prof. Ricardo A. Margni. Universidad de Buenos Aires. Facultad de Farmacia y Bioquímica. Instituto de Estudios de la Inmunidad Humoral Prof. Ricardo A. Margni; Argentina
Fil: Venturiello, Stella Maris. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Houssay. Instituto de Estudios de la Inmunidad Humoral Prof. Ricardo A. Margni. Universidad de Buenos Aires. Facultad de Farmacia y Bioquímica. Instituto de Estudios de la Inmunidad Humoral Prof. Ricardo A. Margni; Argentina
Fil: Pallaro, Anabel Nora. Universidad de Buenos Aires. Facultad de Farmacia y Bioquímica. Departamento de Bromatología y Nutrición Experimental; Argentina
Fil: Baldi, Pablo Cesar. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Houssay. Instituto de Estudios de la Inmunidad Humoral Prof. Ricardo A. Margni. Universidad de Buenos Aires. Facultad de Farmacia y Bioquímica. Instituto de Estudios de la Inmunidad Humoral Prof. Ricardo A. Margni; Argentina
description Objectives: We aimed to analyze the effect of a protein-deficient diet on mucosal and systemic immunity during a Trichinella spiralis infection. Methods: Two groups of weaning Wistar rats received a protein-deficient diet (6.5% casein) and the other two groups received a control diet (20% casein). After 10 d, one group of each diet was infected (PD I and C I ) with muscle larvae (infecting stage). Food intake and body weight were assessed over time. Blood eosinophils counts, antibodies in serum, and tissue extracts were assessed at different days postinfection. Histologic studies were done in the lungs and intestines, and adult worm (AW) fecundity index score and muscle parasite burden were determined. Results: Food and protein intake were lower in PD I than in C I. Body weight was lower in PD I than in a non-infected protein-deficient diet. Eosinophils counts were lower in PD I than in C I. Total and specific antibodies were lower in PD I than C I. PD I had a reduced number of mast and goblet cells in the lungs and intestines compared with C I. The persistence of AW in the intestines and migrant larvae at the lungs was longer in PD I than in C I. . The AW fecundity index score was higher in PD I than in C I . Finally, PD I evidenced a higher muscular parasite burden than C I . Conclusions: Protein deficiency affects the mucosal and systemic immune response to Trichinella spiralis and delays the expulsion and increases the fecundity index score of AW, which leads to a higher parasite burden in the muscles.
publishDate 2019
dc.date.none.fl_str_mv 2019-04
dc.type.none.fl_str_mv info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
http://purl.org/coar/resource_type/c_6501
info:ar-repo/semantics/articulo
format article
status_str publishedVersion
dc.identifier.none.fl_str_mv http://hdl.handle.net/11336/130329
Vila, Cecilia Celeste; Saracino, María Priscila; Falduto, Guido Hernán; Calcagno, Marcela Adriana; Venturiello, Stella Maris; et al.; Protein malnutrition impairs the immune control of Trichinella spiralis infection; Elsevier Science Inc; Nutrition; 60; 4-2019; 161-169
0899-9007
CONICET Digital
CONICET
url http://hdl.handle.net/11336/130329
identifier_str_mv Vila, Cecilia Celeste; Saracino, María Priscila; Falduto, Guido Hernán; Calcagno, Marcela Adriana; Venturiello, Stella Maris; et al.; Protein malnutrition impairs the immune control of Trichinella spiralis infection; Elsevier Science Inc; Nutrition; 60; 4-2019; 161-169
0899-9007
CONICET Digital
CONICET
dc.language.none.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv info:eu-repo/semantics/altIdentifier/url/https://www.sciencedirect.com/science/article/abs/pii/S0899900718308955
info:eu-repo/semantics/altIdentifier/doi/10.1016/j.nut.2018.10.024
dc.rights.none.fl_str_mv info:eu-repo/semantics/openAccess
https://creativecommons.org/licenses/by-nc-nd/2.5/ar/
eu_rights_str_mv openAccess
rights_invalid_str_mv https://creativecommons.org/licenses/by-nc-nd/2.5/ar/
dc.format.none.fl_str_mv application/pdf
application/pdf
application/pdf
application/pdf
dc.publisher.none.fl_str_mv Elsevier Science Inc
publisher.none.fl_str_mv Elsevier Science Inc
dc.source.none.fl_str_mv reponame:CONICET Digital (CONICET)
instname:Consejo Nacional de Investigaciones Científicas y Técnicas
reponame_str CONICET Digital (CONICET)
collection CONICET Digital (CONICET)
instname_str Consejo Nacional de Investigaciones Científicas y Técnicas
repository.name.fl_str_mv CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicas
repository.mail.fl_str_mv dasensio@conicet.gov.ar; lcarlino@conicet.gov.ar
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score 13.001348

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