Dehydroleucodine inhibits mitotic clonal expansion during adipogenesis through cell cycle arrest

Autores
Abood, S.; Veisaga, M. L.; Lopez, Luis Alberto; Barbieri, Maria Araceli
Año de publicación
2018
Idioma
inglés
Tipo de recurso
artículo
Estado
versión publicada
Descripción
Aberrant levels of preadipocyte differentiation, triggered by adipocyte hyperplasia and hypertrophy, results in the obesogenic phenotype. Obesity is a risk factor for several metabolic disorders. In this paper, dehydroleucodine inhibited the accumulation of lipid droplets and decreased the elevations of triglycerides, and this inhibitory effect occurred during the early stage of adipogenesis. Thus, not only did dehydroleucodine downregulate the expression of C/EBPα and PPARγ, it also strongly blocked the expression of C/EBPβ, an early stage biomarker of early adipogenesis, in a concentration-dependent manner. The proliferation of preadipocytes was dramatically suppressed when dehydroleucodine was added to the medium as early as 24 hr. These results indicate that dehydroleucodine may specifically affect mitotic clonal expansion to inhibit preadipocyte differentiation. Dehydroleucodine arrested the cell cycle at the G0/G1 phase, increased p27 and decreased both cyclins A and D and their partners (e.g., CDK2 and CDK4). Additionally, dehydroleucodine decreased phosphorylation of Erk1/2 and Akt. Furthermore, dehydroleucodine downregulated expression of histone demethylase JMJD2B as well as repressed the expression of histone methyltransferase MLL4, which in turn diminished the expression of C/EBPβ and PPARγ, respectively. Collectively, our results indicate that dehydroleucodine inhibits preadipocyte differentiation by blocking mitotic clonal expansion via cell cycle arrest, which may be mediated by regulation of selective histone methylation/demethylation in transcription activation during the early step of adipogenesis.
Fil: Abood, S.. Florida International University; Estados Unidos
Fil: Veisaga, M. L.. Florida International University; Estados Unidos
Fil: Lopez, Luis Alberto. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Mendoza. Instituto de Histología y Embriología de Mendoza Dr. Mario H. Burgos. Universidad Nacional de Cuyo. Facultad de Ciencias Médicas. Instituto de Histología y Embriología de Mendoza Dr. Mario H. Burgos; Argentina
Fil: Barbieri, Maria Araceli. Fairchild Tropical Botanic Garden; . Florida International University; Estados Unidos
Materia
ADIPOGENESIS
C/EBPΑ
C/EBPΒ
MITOTIC CLONAL EXPANSION
PPARΓ
SESQUITERPENE LACTONE
Nivel de accesibilidad
acceso abierto
Condiciones de uso
https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
Repositorio
CONICET Digital (CONICET)
Institución
Consejo Nacional de Investigaciones Científicas y Técnicas
OAI Identificador
oai:ri.conicet.gov.ar:11336/91028
Acceder
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oai_identifier_str oai:ri.conicet.gov.ar:11336/91028
network_acronym_str CONICETDig
repository_id_str 3498
network_name_str CONICET Digital (CONICET)
spelling Dehydroleucodine inhibits mitotic clonal expansion during adipogenesis through cell cycle arrestAbood, S.Veisaga, M. L.Lopez, Luis AlbertoBarbieri, Maria AraceliADIPOGENESISC/EBPΑC/EBPΒMITOTIC CLONAL EXPANSIONPPARΓSESQUITERPENE LACTONEhttps://purl.org/becyt/ford/1.6https://purl.org/becyt/ford/1Aberrant levels of preadipocyte differentiation, triggered by adipocyte hyperplasia and hypertrophy, results in the obesogenic phenotype. Obesity is a risk factor for several metabolic disorders. In this paper, dehydroleucodine inhibited the accumulation of lipid droplets and decreased the elevations of triglycerides, and this inhibitory effect occurred during the early stage of adipogenesis. Thus, not only did dehydroleucodine downregulate the expression of C/EBPα and PPARγ, it also strongly blocked the expression of C/EBPβ, an early stage biomarker of early adipogenesis, in a concentration-dependent manner. The proliferation of preadipocytes was dramatically suppressed when dehydroleucodine was added to the medium as early as 24 hr. These results indicate that dehydroleucodine may specifically affect mitotic clonal expansion to inhibit preadipocyte differentiation. Dehydroleucodine arrested the cell cycle at the G0/G1 phase, increased p27 and decreased both cyclins A and D and their partners (e.g., CDK2 and CDK4). Additionally, dehydroleucodine decreased phosphorylation of Erk1/2 and Akt. Furthermore, dehydroleucodine downregulated expression of histone demethylase JMJD2B as well as repressed the expression of histone methyltransferase MLL4, which in turn diminished the expression of C/EBPβ and PPARγ, respectively. Collectively, our results indicate that dehydroleucodine inhibits preadipocyte differentiation by blocking mitotic clonal expansion via cell cycle arrest, which may be mediated by regulation of selective histone methylation/demethylation in transcription activation during the early step of adipogenesis.Fil: Abood, S.. Florida International University; Estados UnidosFil: Veisaga, M. L.. Florida International University; Estados UnidosFil: Lopez, Luis Alberto. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Mendoza. Instituto de Histología y Embriología de Mendoza Dr. Mario H. Burgos. Universidad Nacional de Cuyo. Facultad de Ciencias Médicas. Instituto de Histología y Embriología de Mendoza Dr. Mario H. Burgos; ArgentinaFil: Barbieri, Maria Araceli. Fairchild Tropical Botanic Garden; . Florida International University; Estados UnidosJohn Wiley & Sons Ltd2018-08info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionhttp://purl.org/coar/resource_type/c_6501info:ar-repo/semantics/articuloapplication/pdfapplication/pdfapplication/pdfhttp://hdl.handle.net/11336/91028Abood, S.; Veisaga, M. L.; Lopez, Luis Alberto; Barbieri, Maria Araceli; Dehydroleucodine inhibits mitotic clonal expansion during adipogenesis through cell cycle arrest; John Wiley & Sons Ltd; Phytotherapy Research; 32; 8; 8-2018; 1583-15920951-418XCONICET DigitalCONICETenginfo:eu-repo/semantics/altIdentifier/doi/10.1002/ptr.6089info:eu-repo/semantics/altIdentifier/url/https://onlinelibrary.wiley.com/doi/full/10.1002/ptr.6089info:eu-repo/semantics/openAccesshttps://creativecommons.org/licenses/by-nc-sa/2.5/ar/reponame:CONICET Digital (CONICET)instname:Consejo Nacional de Investigaciones Científicas y Técnicas2025-10-15T14:28:19Zoai:ri.conicet.gov.ar:11336/91028instacron:CONICETInstitucionalhttp://ri.conicet.gov.ar/Organismo científico-tecnológicoNo correspondehttp://ri.conicet.gov.ar/oai/requestdasensio@conicet.gov.ar; lcarlino@conicet.gov.arArgentinaNo correspondeNo correspondeNo correspondeopendoar:34982025-10-15 14:28:19.627CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicasfalse
dc.title.none.fl_str_mv Dehydroleucodine inhibits mitotic clonal expansion during adipogenesis through cell cycle arrest
title Dehydroleucodine inhibits mitotic clonal expansion during adipogenesis through cell cycle arrest
spellingShingle Dehydroleucodine inhibits mitotic clonal expansion during adipogenesis through cell cycle arrest
Abood, S.
ADIPOGENESIS
C/EBPΑ
C/EBPΒ
MITOTIC CLONAL EXPANSION
PPARΓ
SESQUITERPENE LACTONE
title_short Dehydroleucodine inhibits mitotic clonal expansion during adipogenesis through cell cycle arrest
title_full Dehydroleucodine inhibits mitotic clonal expansion during adipogenesis through cell cycle arrest
title_fullStr Dehydroleucodine inhibits mitotic clonal expansion during adipogenesis through cell cycle arrest
title_full_unstemmed Dehydroleucodine inhibits mitotic clonal expansion during adipogenesis through cell cycle arrest
title_sort Dehydroleucodine inhibits mitotic clonal expansion during adipogenesis through cell cycle arrest
dc.creator.none.fl_str_mv Abood, S.
Veisaga, M. L.
Lopez, Luis Alberto
Barbieri, Maria Araceli
author Abood, S.
author_facet Abood, S.
Veisaga, M. L.
Lopez, Luis Alberto
Barbieri, Maria Araceli
author_role author
author2 Veisaga, M. L.
Lopez, Luis Alberto
Barbieri, Maria Araceli
author2_role author
author
author
dc.subject.none.fl_str_mv ADIPOGENESIS
C/EBPΑ
C/EBPΒ
MITOTIC CLONAL EXPANSION
PPARΓ
SESQUITERPENE LACTONE
topic ADIPOGENESIS
C/EBPΑ
C/EBPΒ
MITOTIC CLONAL EXPANSION
PPARΓ
SESQUITERPENE LACTONE
purl_subject.fl_str_mv https://purl.org/becyt/ford/1.6
https://purl.org/becyt/ford/1
dc.description.none.fl_txt_mv Aberrant levels of preadipocyte differentiation, triggered by adipocyte hyperplasia and hypertrophy, results in the obesogenic phenotype. Obesity is a risk factor for several metabolic disorders. In this paper, dehydroleucodine inhibited the accumulation of lipid droplets and decreased the elevations of triglycerides, and this inhibitory effect occurred during the early stage of adipogenesis. Thus, not only did dehydroleucodine downregulate the expression of C/EBPα and PPARγ, it also strongly blocked the expression of C/EBPβ, an early stage biomarker of early adipogenesis, in a concentration-dependent manner. The proliferation of preadipocytes was dramatically suppressed when dehydroleucodine was added to the medium as early as 24 hr. These results indicate that dehydroleucodine may specifically affect mitotic clonal expansion to inhibit preadipocyte differentiation. Dehydroleucodine arrested the cell cycle at the G0/G1 phase, increased p27 and decreased both cyclins A and D and their partners (e.g., CDK2 and CDK4). Additionally, dehydroleucodine decreased phosphorylation of Erk1/2 and Akt. Furthermore, dehydroleucodine downregulated expression of histone demethylase JMJD2B as well as repressed the expression of histone methyltransferase MLL4, which in turn diminished the expression of C/EBPβ and PPARγ, respectively. Collectively, our results indicate that dehydroleucodine inhibits preadipocyte differentiation by blocking mitotic clonal expansion via cell cycle arrest, which may be mediated by regulation of selective histone methylation/demethylation in transcription activation during the early step of adipogenesis.
Fil: Abood, S.. Florida International University; Estados Unidos
Fil: Veisaga, M. L.. Florida International University; Estados Unidos
Fil: Lopez, Luis Alberto. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Mendoza. Instituto de Histología y Embriología de Mendoza Dr. Mario H. Burgos. Universidad Nacional de Cuyo. Facultad de Ciencias Médicas. Instituto de Histología y Embriología de Mendoza Dr. Mario H. Burgos; Argentina
Fil: Barbieri, Maria Araceli. Fairchild Tropical Botanic Garden; . Florida International University; Estados Unidos
description Aberrant levels of preadipocyte differentiation, triggered by adipocyte hyperplasia and hypertrophy, results in the obesogenic phenotype. Obesity is a risk factor for several metabolic disorders. In this paper, dehydroleucodine inhibited the accumulation of lipid droplets and decreased the elevations of triglycerides, and this inhibitory effect occurred during the early stage of adipogenesis. Thus, not only did dehydroleucodine downregulate the expression of C/EBPα and PPARγ, it also strongly blocked the expression of C/EBPβ, an early stage biomarker of early adipogenesis, in a concentration-dependent manner. The proliferation of preadipocytes was dramatically suppressed when dehydroleucodine was added to the medium as early as 24 hr. These results indicate that dehydroleucodine may specifically affect mitotic clonal expansion to inhibit preadipocyte differentiation. Dehydroleucodine arrested the cell cycle at the G0/G1 phase, increased p27 and decreased both cyclins A and D and their partners (e.g., CDK2 and CDK4). Additionally, dehydroleucodine decreased phosphorylation of Erk1/2 and Akt. Furthermore, dehydroleucodine downregulated expression of histone demethylase JMJD2B as well as repressed the expression of histone methyltransferase MLL4, which in turn diminished the expression of C/EBPβ and PPARγ, respectively. Collectively, our results indicate that dehydroleucodine inhibits preadipocyte differentiation by blocking mitotic clonal expansion via cell cycle arrest, which may be mediated by regulation of selective histone methylation/demethylation in transcription activation during the early step of adipogenesis.
publishDate 2018
dc.date.none.fl_str_mv 2018-08
dc.type.none.fl_str_mv info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
http://purl.org/coar/resource_type/c_6501
info:ar-repo/semantics/articulo
format article
status_str publishedVersion
dc.identifier.none.fl_str_mv http://hdl.handle.net/11336/91028
Abood, S.; Veisaga, M. L.; Lopez, Luis Alberto; Barbieri, Maria Araceli; Dehydroleucodine inhibits mitotic clonal expansion during adipogenesis through cell cycle arrest; John Wiley & Sons Ltd; Phytotherapy Research; 32; 8; 8-2018; 1583-1592
0951-418X
CONICET Digital
CONICET
url http://hdl.handle.net/11336/91028
identifier_str_mv Abood, S.; Veisaga, M. L.; Lopez, Luis Alberto; Barbieri, Maria Araceli; Dehydroleucodine inhibits mitotic clonal expansion during adipogenesis through cell cycle arrest; John Wiley & Sons Ltd; Phytotherapy Research; 32; 8; 8-2018; 1583-1592
0951-418X
CONICET Digital
CONICET
dc.language.none.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv info:eu-repo/semantics/altIdentifier/doi/10.1002/ptr.6089
info:eu-repo/semantics/altIdentifier/url/https://onlinelibrary.wiley.com/doi/full/10.1002/ptr.6089
dc.rights.none.fl_str_mv info:eu-repo/semantics/openAccess
https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
eu_rights_str_mv openAccess
rights_invalid_str_mv https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
dc.format.none.fl_str_mv application/pdf
application/pdf
application/pdf
dc.publisher.none.fl_str_mv John Wiley & Sons Ltd
publisher.none.fl_str_mv John Wiley & Sons Ltd
dc.source.none.fl_str_mv reponame:CONICET Digital (CONICET)
instname:Consejo Nacional de Investigaciones Científicas y Técnicas
reponame_str CONICET Digital (CONICET)
collection CONICET Digital (CONICET)
instname_str Consejo Nacional de Investigaciones Científicas y Técnicas
repository.name.fl_str_mv CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicas
repository.mail.fl_str_mv dasensio@conicet.gov.ar; lcarlino@conicet.gov.ar
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score 13.22299

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