Origin of recognition complex 3 controls the development of maternal excess endosperm in the Paspalum simplex agamic complex ( Poaceae )

Autores
Bellucci, Michele; Caceres, Maria Eugenia; Paolocci, Francesco; Vega, Juan Manuel; Ortiz, Juan Pablo Amelio; Ceccarelli, Marilena; De Marchis, Francesca; Pupilli, Fulvio
Año de publicación
2023
Idioma
inglés
Tipo de recurso
artículo
Estado
versión publicada
Descripción
Pseudogamous apomixis in Paspalum simplex generates seeds with embryos genetically identical to the mother plant and endosperms deviating from the canonical 2(maternal):1(paternal) parental genome contribution into a maternal excess 4m:1p genome ratio. In P. simplex the gene homologous to the one coding for the subunit 3 of the ORIGIN OF RECOGNITION COMPLEX (PsORC3) exists in three isogenic forms: PsORC3a is apomixis-specific and constitutively expressed in developing endosperm whereas PsORCb and PsORCc are up regulated in sexual endosperms and silenced in apomictic ones. This raises the question of how the different arrangement and expression profiles of these three ORC3 isogenes are linked to seed development in interploidy crosses generating maternal excess endosperms. We demonstrate that the PsORC3b downregulation in sexual tetraploid plants is sufficient to restore seed fertility in interploidy 4n x 2n crosses and, in turn, its expression level at the transition from proliferating to endoreduplication endosperm developmental stages dictates the fate of these seeds. Furthermore, we show that only when being maternally inherited, PsORC3c can up-regulate PsORC3b. Our findings lay the basis for an innovative route - based on ORC3 manipulation - to introgress the apomictic trait into sexual crops and overcome the fertilization barriers in interploidy crosses.
Fil: Bellucci, Michele. Centre National de la Recherche Scientifique; Francia
Fil: Caceres, Maria Eugenia. Centre National de la Recherche Scientifique; Francia
Fil: Paolocci, Francesco. Centre National de la Recherche Scientifique; Francia
Fil: Vega, Juan Manuel. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Rosario. Instituto de Investigaciones en Ciencias Agrarias de Rosario. Universidad Nacional de Rosario. Facultad de Ciencias Agrarias. Instituto de Investigaciones en Ciencias Agrarias de Rosario; Argentina
Fil: Ortiz, Juan Pablo Amelio. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Rosario. Instituto de Investigaciones en Ciencias Agrarias de Rosario. Universidad Nacional de Rosario. Facultad de Ciencias Agrarias. Instituto de Investigaciones en Ciencias Agrarias de Rosario; Argentina
Fil: Ceccarelli, Marilena. Università di Perugia; Italia
Fil: De Marchis, Francesca. Centre National de la Recherche Scientifique; Francia
Fil: Pupilli, Fulvio. Centre National de la Recherche Scientifique; Francia
Materia
apomixis
interploidy crosses
maternal excess endosperm
Paspalum simplex
Nivel de accesibilidad
acceso abierto
Condiciones de uso
https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
Repositorio
CONICET Digital (CONICET)
Institución
Consejo Nacional de Investigaciones Científicas y Técnicas
OAI Identificador
oai:ri.conicet.gov.ar:11336/244553
Acceder
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oai_identifier_str oai:ri.conicet.gov.ar:11336/244553
network_acronym_str CONICETDig
repository_id_str 3498
network_name_str CONICET Digital (CONICET)
spelling Origin of recognition complex 3 controls the development of maternal excess endosperm in the Paspalum simplex agamic complex ( Poaceae )Bellucci, MicheleCaceres, Maria EugeniaPaolocci, FrancescoVega, Juan ManuelOrtiz, Juan Pablo AmelioCeccarelli, MarilenaDe Marchis, FrancescaPupilli, Fulvioapomixisinterploidy crossesmaternal excess endospermPaspalum simplexhttps://purl.org/becyt/ford/1.6https://purl.org/becyt/ford/1Pseudogamous apomixis in Paspalum simplex generates seeds with embryos genetically identical to the mother plant and endosperms deviating from the canonical 2(maternal):1(paternal) parental genome contribution into a maternal excess 4m:1p genome ratio. In P. simplex the gene homologous to the one coding for the subunit 3 of the ORIGIN OF RECOGNITION COMPLEX (PsORC3) exists in three isogenic forms: PsORC3a is apomixis-specific and constitutively expressed in developing endosperm whereas PsORCb and PsORCc are up regulated in sexual endosperms and silenced in apomictic ones. This raises the question of how the different arrangement and expression profiles of these three ORC3 isogenes are linked to seed development in interploidy crosses generating maternal excess endosperms. We demonstrate that the PsORC3b downregulation in sexual tetraploid plants is sufficient to restore seed fertility in interploidy 4n x 2n crosses and, in turn, its expression level at the transition from proliferating to endoreduplication endosperm developmental stages dictates the fate of these seeds. Furthermore, we show that only when being maternally inherited, PsORC3c can up-regulate PsORC3b. Our findings lay the basis for an innovative route - based on ORC3 manipulation - to introgress the apomictic trait into sexual crops and overcome the fertilization barriers in interploidy crosses.Fil: Bellucci, Michele. Centre National de la Recherche Scientifique; FranciaFil: Caceres, Maria Eugenia. Centre National de la Recherche Scientifique; FranciaFil: Paolocci, Francesco. Centre National de la Recherche Scientifique; FranciaFil: Vega, Juan Manuel. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Rosario. Instituto de Investigaciones en Ciencias Agrarias de Rosario. Universidad Nacional de Rosario. Facultad de Ciencias Agrarias. Instituto de Investigaciones en Ciencias Agrarias de Rosario; ArgentinaFil: Ortiz, Juan Pablo Amelio. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Rosario. Instituto de Investigaciones en Ciencias Agrarias de Rosario. Universidad Nacional de Rosario. Facultad de Ciencias Agrarias. Instituto de Investigaciones en Ciencias Agrarias de Rosario; ArgentinaFil: Ceccarelli, Marilena. Università di Perugia; ItaliaFil: De Marchis, Francesca. Centre National de la Recherche Scientifique; FranciaFil: Pupilli, Fulvio. Centre National de la Recherche Scientifique; FranciaOxford University Press2023-05info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionhttp://purl.org/coar/resource_type/c_6501info:ar-repo/semantics/articuloapplication/pdfapplication/pdfhttp://hdl.handle.net/11336/244553Bellucci, Michele; Caceres, Maria Eugenia; Paolocci, Francesco; Vega, Juan Manuel; Ortiz, Juan Pablo Amelio; et al.; Origin of recognition complex 3 controls the development of maternal excess endosperm in the Paspalum simplex agamic complex ( Poaceae ); Oxford University Press; Journal of Experimental Botany; 74; 10; 5-2023; 3074-30930022-0957CONICET DigitalCONICETenginfo:eu-repo/semantics/altIdentifier/url/https://academic.oup.com/jxb/advance-article/doi/10.1093/jxb/erad069/7051163info:eu-repo/semantics/altIdentifier/doi/10.1093/jxb/erad069info:eu-repo/semantics/openAccesshttps://creativecommons.org/licenses/by-nc-sa/2.5/ar/reponame:CONICET Digital (CONICET)instname:Consejo Nacional de Investigaciones Científicas y Técnicas2025-09-29T10:00:33Zoai:ri.conicet.gov.ar:11336/244553instacron:CONICETInstitucionalhttp://ri.conicet.gov.ar/Organismo científico-tecnológicoNo correspondehttp://ri.conicet.gov.ar/oai/requestdasensio@conicet.gov.ar; lcarlino@conicet.gov.arArgentinaNo correspondeNo correspondeNo correspondeopendoar:34982025-09-29 10:00:33.389CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicasfalse
dc.title.none.fl_str_mv Origin of recognition complex 3 controls the development of maternal excess endosperm in the Paspalum simplex agamic complex ( Poaceae )
title Origin of recognition complex 3 controls the development of maternal excess endosperm in the Paspalum simplex agamic complex ( Poaceae )
spellingShingle Origin of recognition complex 3 controls the development of maternal excess endosperm in the Paspalum simplex agamic complex ( Poaceae )
Bellucci, Michele
apomixis
interploidy crosses
maternal excess endosperm
Paspalum simplex
title_short Origin of recognition complex 3 controls the development of maternal excess endosperm in the Paspalum simplex agamic complex ( Poaceae )
title_full Origin of recognition complex 3 controls the development of maternal excess endosperm in the Paspalum simplex agamic complex ( Poaceae )
title_fullStr Origin of recognition complex 3 controls the development of maternal excess endosperm in the Paspalum simplex agamic complex ( Poaceae )
title_full_unstemmed Origin of recognition complex 3 controls the development of maternal excess endosperm in the Paspalum simplex agamic complex ( Poaceae )
title_sort Origin of recognition complex 3 controls the development of maternal excess endosperm in the Paspalum simplex agamic complex ( Poaceae )
dc.creator.none.fl_str_mv Bellucci, Michele
Caceres, Maria Eugenia
Paolocci, Francesco
Vega, Juan Manuel
Ortiz, Juan Pablo Amelio
Ceccarelli, Marilena
De Marchis, Francesca
Pupilli, Fulvio
author Bellucci, Michele
author_facet Bellucci, Michele
Caceres, Maria Eugenia
Paolocci, Francesco
Vega, Juan Manuel
Ortiz, Juan Pablo Amelio
Ceccarelli, Marilena
De Marchis, Francesca
Pupilli, Fulvio
author_role author
author2 Caceres, Maria Eugenia
Paolocci, Francesco
Vega, Juan Manuel
Ortiz, Juan Pablo Amelio
Ceccarelli, Marilena
De Marchis, Francesca
Pupilli, Fulvio
author2_role author
author
author
author
author
author
author
dc.subject.none.fl_str_mv apomixis
interploidy crosses
maternal excess endosperm
Paspalum simplex
topic apomixis
interploidy crosses
maternal excess endosperm
Paspalum simplex
purl_subject.fl_str_mv https://purl.org/becyt/ford/1.6
https://purl.org/becyt/ford/1
dc.description.none.fl_txt_mv Pseudogamous apomixis in Paspalum simplex generates seeds with embryos genetically identical to the mother plant and endosperms deviating from the canonical 2(maternal):1(paternal) parental genome contribution into a maternal excess 4m:1p genome ratio. In P. simplex the gene homologous to the one coding for the subunit 3 of the ORIGIN OF RECOGNITION COMPLEX (PsORC3) exists in three isogenic forms: PsORC3a is apomixis-specific and constitutively expressed in developing endosperm whereas PsORCb and PsORCc are up regulated in sexual endosperms and silenced in apomictic ones. This raises the question of how the different arrangement and expression profiles of these three ORC3 isogenes are linked to seed development in interploidy crosses generating maternal excess endosperms. We demonstrate that the PsORC3b downregulation in sexual tetraploid plants is sufficient to restore seed fertility in interploidy 4n x 2n crosses and, in turn, its expression level at the transition from proliferating to endoreduplication endosperm developmental stages dictates the fate of these seeds. Furthermore, we show that only when being maternally inherited, PsORC3c can up-regulate PsORC3b. Our findings lay the basis for an innovative route - based on ORC3 manipulation - to introgress the apomictic trait into sexual crops and overcome the fertilization barriers in interploidy crosses.
Fil: Bellucci, Michele. Centre National de la Recherche Scientifique; Francia
Fil: Caceres, Maria Eugenia. Centre National de la Recherche Scientifique; Francia
Fil: Paolocci, Francesco. Centre National de la Recherche Scientifique; Francia
Fil: Vega, Juan Manuel. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Rosario. Instituto de Investigaciones en Ciencias Agrarias de Rosario. Universidad Nacional de Rosario. Facultad de Ciencias Agrarias. Instituto de Investigaciones en Ciencias Agrarias de Rosario; Argentina
Fil: Ortiz, Juan Pablo Amelio. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Rosario. Instituto de Investigaciones en Ciencias Agrarias de Rosario. Universidad Nacional de Rosario. Facultad de Ciencias Agrarias. Instituto de Investigaciones en Ciencias Agrarias de Rosario; Argentina
Fil: Ceccarelli, Marilena. Università di Perugia; Italia
Fil: De Marchis, Francesca. Centre National de la Recherche Scientifique; Francia
Fil: Pupilli, Fulvio. Centre National de la Recherche Scientifique; Francia
description Pseudogamous apomixis in Paspalum simplex generates seeds with embryos genetically identical to the mother plant and endosperms deviating from the canonical 2(maternal):1(paternal) parental genome contribution into a maternal excess 4m:1p genome ratio. In P. simplex the gene homologous to the one coding for the subunit 3 of the ORIGIN OF RECOGNITION COMPLEX (PsORC3) exists in three isogenic forms: PsORC3a is apomixis-specific and constitutively expressed in developing endosperm whereas PsORCb and PsORCc are up regulated in sexual endosperms and silenced in apomictic ones. This raises the question of how the different arrangement and expression profiles of these three ORC3 isogenes are linked to seed development in interploidy crosses generating maternal excess endosperms. We demonstrate that the PsORC3b downregulation in sexual tetraploid plants is sufficient to restore seed fertility in interploidy 4n x 2n crosses and, in turn, its expression level at the transition from proliferating to endoreduplication endosperm developmental stages dictates the fate of these seeds. Furthermore, we show that only when being maternally inherited, PsORC3c can up-regulate PsORC3b. Our findings lay the basis for an innovative route - based on ORC3 manipulation - to introgress the apomictic trait into sexual crops and overcome the fertilization barriers in interploidy crosses.
publishDate 2023
dc.date.none.fl_str_mv 2023-05
dc.type.none.fl_str_mv info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
http://purl.org/coar/resource_type/c_6501
info:ar-repo/semantics/articulo
format article
status_str publishedVersion
dc.identifier.none.fl_str_mv http://hdl.handle.net/11336/244553
Bellucci, Michele; Caceres, Maria Eugenia; Paolocci, Francesco; Vega, Juan Manuel; Ortiz, Juan Pablo Amelio; et al.; Origin of recognition complex 3 controls the development of maternal excess endosperm in the Paspalum simplex agamic complex ( Poaceae ); Oxford University Press; Journal of Experimental Botany; 74; 10; 5-2023; 3074-3093
0022-0957
CONICET Digital
CONICET
url http://hdl.handle.net/11336/244553
identifier_str_mv Bellucci, Michele; Caceres, Maria Eugenia; Paolocci, Francesco; Vega, Juan Manuel; Ortiz, Juan Pablo Amelio; et al.; Origin of recognition complex 3 controls the development of maternal excess endosperm in the Paspalum simplex agamic complex ( Poaceae ); Oxford University Press; Journal of Experimental Botany; 74; 10; 5-2023; 3074-3093
0022-0957
CONICET Digital
CONICET
dc.language.none.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv info:eu-repo/semantics/altIdentifier/url/https://academic.oup.com/jxb/advance-article/doi/10.1093/jxb/erad069/7051163
info:eu-repo/semantics/altIdentifier/doi/10.1093/jxb/erad069
dc.rights.none.fl_str_mv info:eu-repo/semantics/openAccess
https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
eu_rights_str_mv openAccess
rights_invalid_str_mv https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
dc.format.none.fl_str_mv application/pdf
application/pdf
dc.publisher.none.fl_str_mv Oxford University Press
publisher.none.fl_str_mv Oxford University Press
dc.source.none.fl_str_mv reponame:CONICET Digital (CONICET)
instname:Consejo Nacional de Investigaciones Científicas y Técnicas
reponame_str CONICET Digital (CONICET)
collection CONICET Digital (CONICET)
instname_str Consejo Nacional de Investigaciones Científicas y Técnicas
repository.name.fl_str_mv CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicas
repository.mail.fl_str_mv dasensio@conicet.gov.ar; lcarlino@conicet.gov.ar
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score 13.070432

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