Galectin-3 negatively regulates the frequency and function of CD4+CD25+Foxp3+ regulatory T cells and influences the course of Leishmania major infection
- Autores
- Fermino, Marise L.; Dias, Fabrício C.; Lopes, Carla D.; Souza, Maria A.; Cruz, Ângela K.; Liu, Fu Tong; Chammas, Roger; Roque Barreira, Maria C.; Rabinovich, Gabriel Adrian; Bernardes, Emerson S.
- Año de publicación
- 2013
- Idioma
- inglés
- Tipo de recurso
- artículo
- Estado
- versión publicada
- Descripción
- Galectin-3, an endogenous glycan-binding protein, plays essential roles during microbial infection by modulating innate and adaptive immunity. However, the role of galectin-3 within the CD4+CD25+Foxp3+ T regulatory (TREG) cell compartment has not yet been explored. Here, we found, in a model of Leishmania major infection, that galectin-3 deficiency increases the frequency of peripheral TREG cells both in draining lymph nodes (LNs) and sites of infection. These observations correlated with an increased severity of the disease, as shown by increased footpad swelling and parasite burden. Galectin-3-deficient (Lgals3−/−) TREG cells displayed higher CD103 expression, showed greater suppressive capacity, and synthesized higher amounts of IL-10 compared with their wild-type (WT) counterpart. Furthermore, both TREG cells and T effector (TEFF) cells from Lgals3−/− mice showed higher expression of Notch1 and the Notch target gene Hes-1. Interestingly, Notch signaling components were also altered in both TREG and TEFF cells from uninfected Lgals3−/− mice. Thus, endogenous galectin-3 regulates the frequency and function of CD4+CD25+Foxp3+ TREG cells and alters the course of L. major infection.
Fil: Fermino, Marise L.. Universidade de Sao Paulo; Brasil
Fil: Dias, Fabrício C.. Universidade de Sao Paulo; Brasil
Fil: Lopes, Carla D.. Universidade de Sao Paulo; Brasil
Fil: Souza, Maria A.. Universidade de Sao Paulo; Brasil
Fil: Cruz, Ângela K.. Universidade de Sao Paulo; Brasil
Fil: Liu, Fu Tong. University of California at Davis; Estados Unidos
Fil: Chammas, Roger. Universidade de Sao Paulo; Brasil
Fil: Roque Barreira, Maria C.. Universidade de Sao Paulo; Brasil
Fil: Rabinovich, Gabriel Adrian. Consejo Nacional de Investigaciones Científicas y Técnicas. Instituto de Biología y Medicina Experimental (i); Argentina. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales. Departamento de Química Biológica; Argentina
Fil: Bernardes, Emerson S.. Universidade de Sao Paulo; Brasil - Materia
-
GALECTIN-3
IL-10
LEISHMANIA MAJOR
NOTCH SIGNALING
T REGULATORY (TREG) CELLS - Nivel de accesibilidad
- acceso abierto
- Condiciones de uso
- https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
- Repositorio
- Institución
- Consejo Nacional de Investigaciones Científicas y Técnicas
- OAI Identificador
- oai:ri.conicet.gov.ar:11336/2356
Ver los metadatos del registro completo
id |
CONICETDig_171fe5f48ac3da582c5e07f1f51e5828 |
---|---|
oai_identifier_str |
oai:ri.conicet.gov.ar:11336/2356 |
network_acronym_str |
CONICETDig |
repository_id_str |
3498 |
network_name_str |
CONICET Digital (CONICET) |
spelling |
Galectin-3 negatively regulates the frequency and function of CD4+CD25+Foxp3+ regulatory T cells and influences the course of Leishmania major infectionFermino, Marise L.Dias, Fabrício C.Lopes, Carla D.Souza, Maria A.Cruz, Ângela K.Liu, Fu TongChammas, RogerRoque Barreira, Maria C.Rabinovich, Gabriel AdrianBernardes, Emerson S.GALECTIN-3IL-10LEISHMANIA MAJORNOTCH SIGNALINGT REGULATORY (TREG) CELLShttps://purl.org/becyt/ford/3.1https://purl.org/becyt/ford/3Galectin-3, an endogenous glycan-binding protein, plays essential roles during microbial infection by modulating innate and adaptive immunity. However, the role of galectin-3 within the CD4+CD25+Foxp3+ T regulatory (TREG) cell compartment has not yet been explored. Here, we found, in a model of Leishmania major infection, that galectin-3 deficiency increases the frequency of peripheral TREG cells both in draining lymph nodes (LNs) and sites of infection. These observations correlated with an increased severity of the disease, as shown by increased footpad swelling and parasite burden. Galectin-3-deficient (Lgals3−/−) TREG cells displayed higher CD103 expression, showed greater suppressive capacity, and synthesized higher amounts of IL-10 compared with their wild-type (WT) counterpart. Furthermore, both TREG cells and T effector (TEFF) cells from Lgals3−/− mice showed higher expression of Notch1 and the Notch target gene Hes-1. Interestingly, Notch signaling components were also altered in both TREG and TEFF cells from uninfected Lgals3−/− mice. Thus, endogenous galectin-3 regulates the frequency and function of CD4+CD25+Foxp3+ TREG cells and alters the course of L. major infection.Fil: Fermino, Marise L.. Universidade de Sao Paulo; BrasilFil: Dias, Fabrício C.. Universidade de Sao Paulo; BrasilFil: Lopes, Carla D.. Universidade de Sao Paulo; BrasilFil: Souza, Maria A.. Universidade de Sao Paulo; BrasilFil: Cruz, Ângela K.. Universidade de Sao Paulo; BrasilFil: Liu, Fu Tong. University of California at Davis; Estados UnidosFil: Chammas, Roger. Universidade de Sao Paulo; BrasilFil: Roque Barreira, Maria C.. Universidade de Sao Paulo; BrasilFil: Rabinovich, Gabriel Adrian. Consejo Nacional de Investigaciones Científicas y Técnicas. Instituto de Biología y Medicina Experimental (i); Argentina. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales. Departamento de Química Biológica; ArgentinaFil: Bernardes, Emerson S.. Universidade de Sao Paulo; BrasilWiley VCH Verlag2013-05-17info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionhttp://purl.org/coar/resource_type/c_6501info:ar-repo/semantics/articuloapplication/pdfapplication/pdfapplication/pdfhttp://hdl.handle.net/11336/2356Fermino, Marise L.; Dias, Fabrício C.; Lopes, Carla D.; Souza, Maria A.; Cruz, Ângela K.; et al.; Galectin-3 negatively regulates the frequency and function of CD4+CD25+Foxp3+ regulatory T cells and influences the course of Leishmania major infection; Wiley VCH Verlag; European Journal of Immunology; 43; 7; 17-5-2013; 1806-18170014-29801521-4141enginfo:eu-repo/semantics/altIdentifier/doi/10.1002/eji.201343381info:eu-repo/semantics/altIdentifier/url/http://onlinelibrary.wiley.com/doi/10.1002/eji.201343381/abstract;jsessionid=67113EC5897BBBED63DAF7E40BDBBE33.f03t04info:eu-repo/semantics/openAccesshttps://creativecommons.org/licenses/by-nc-sa/2.5/ar/reponame:CONICET Digital (CONICET)instname:Consejo Nacional de Investigaciones Científicas y Técnicas2025-09-03T09:47:38Zoai:ri.conicet.gov.ar:11336/2356instacron:CONICETInstitucionalhttp://ri.conicet.gov.ar/Organismo científico-tecnológicoNo correspondehttp://ri.conicet.gov.ar/oai/requestdasensio@conicet.gov.ar; lcarlino@conicet.gov.arArgentinaNo correspondeNo correspondeNo correspondeopendoar:34982025-09-03 09:47:39.75CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicasfalse |
dc.title.none.fl_str_mv |
Galectin-3 negatively regulates the frequency and function of CD4+CD25+Foxp3+ regulatory T cells and influences the course of Leishmania major infection |
title |
Galectin-3 negatively regulates the frequency and function of CD4+CD25+Foxp3+ regulatory T cells and influences the course of Leishmania major infection |
spellingShingle |
Galectin-3 negatively regulates the frequency and function of CD4+CD25+Foxp3+ regulatory T cells and influences the course of Leishmania major infection Fermino, Marise L. GALECTIN-3 IL-10 LEISHMANIA MAJOR NOTCH SIGNALING T REGULATORY (TREG) CELLS |
title_short |
Galectin-3 negatively regulates the frequency and function of CD4+CD25+Foxp3+ regulatory T cells and influences the course of Leishmania major infection |
title_full |
Galectin-3 negatively regulates the frequency and function of CD4+CD25+Foxp3+ regulatory T cells and influences the course of Leishmania major infection |
title_fullStr |
Galectin-3 negatively regulates the frequency and function of CD4+CD25+Foxp3+ regulatory T cells and influences the course of Leishmania major infection |
title_full_unstemmed |
Galectin-3 negatively regulates the frequency and function of CD4+CD25+Foxp3+ regulatory T cells and influences the course of Leishmania major infection |
title_sort |
Galectin-3 negatively regulates the frequency and function of CD4+CD25+Foxp3+ regulatory T cells and influences the course of Leishmania major infection |
dc.creator.none.fl_str_mv |
Fermino, Marise L. Dias, Fabrício C. Lopes, Carla D. Souza, Maria A. Cruz, Ângela K. Liu, Fu Tong Chammas, Roger Roque Barreira, Maria C. Rabinovich, Gabriel Adrian Bernardes, Emerson S. |
author |
Fermino, Marise L. |
author_facet |
Fermino, Marise L. Dias, Fabrício C. Lopes, Carla D. Souza, Maria A. Cruz, Ângela K. Liu, Fu Tong Chammas, Roger Roque Barreira, Maria C. Rabinovich, Gabriel Adrian Bernardes, Emerson S. |
author_role |
author |
author2 |
Dias, Fabrício C. Lopes, Carla D. Souza, Maria A. Cruz, Ângela K. Liu, Fu Tong Chammas, Roger Roque Barreira, Maria C. Rabinovich, Gabriel Adrian Bernardes, Emerson S. |
author2_role |
author author author author author author author author author |
dc.subject.none.fl_str_mv |
GALECTIN-3 IL-10 LEISHMANIA MAJOR NOTCH SIGNALING T REGULATORY (TREG) CELLS |
topic |
GALECTIN-3 IL-10 LEISHMANIA MAJOR NOTCH SIGNALING T REGULATORY (TREG) CELLS |
purl_subject.fl_str_mv |
https://purl.org/becyt/ford/3.1 https://purl.org/becyt/ford/3 |
dc.description.none.fl_txt_mv |
Galectin-3, an endogenous glycan-binding protein, plays essential roles during microbial infection by modulating innate and adaptive immunity. However, the role of galectin-3 within the CD4+CD25+Foxp3+ T regulatory (TREG) cell compartment has not yet been explored. Here, we found, in a model of Leishmania major infection, that galectin-3 deficiency increases the frequency of peripheral TREG cells both in draining lymph nodes (LNs) and sites of infection. These observations correlated with an increased severity of the disease, as shown by increased footpad swelling and parasite burden. Galectin-3-deficient (Lgals3−/−) TREG cells displayed higher CD103 expression, showed greater suppressive capacity, and synthesized higher amounts of IL-10 compared with their wild-type (WT) counterpart. Furthermore, both TREG cells and T effector (TEFF) cells from Lgals3−/− mice showed higher expression of Notch1 and the Notch target gene Hes-1. Interestingly, Notch signaling components were also altered in both TREG and TEFF cells from uninfected Lgals3−/− mice. Thus, endogenous galectin-3 regulates the frequency and function of CD4+CD25+Foxp3+ TREG cells and alters the course of L. major infection. Fil: Fermino, Marise L.. Universidade de Sao Paulo; Brasil Fil: Dias, Fabrício C.. Universidade de Sao Paulo; Brasil Fil: Lopes, Carla D.. Universidade de Sao Paulo; Brasil Fil: Souza, Maria A.. Universidade de Sao Paulo; Brasil Fil: Cruz, Ângela K.. Universidade de Sao Paulo; Brasil Fil: Liu, Fu Tong. University of California at Davis; Estados Unidos Fil: Chammas, Roger. Universidade de Sao Paulo; Brasil Fil: Roque Barreira, Maria C.. Universidade de Sao Paulo; Brasil Fil: Rabinovich, Gabriel Adrian. Consejo Nacional de Investigaciones Científicas y Técnicas. Instituto de Biología y Medicina Experimental (i); Argentina. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales. Departamento de Química Biológica; Argentina Fil: Bernardes, Emerson S.. Universidade de Sao Paulo; Brasil |
description |
Galectin-3, an endogenous glycan-binding protein, plays essential roles during microbial infection by modulating innate and adaptive immunity. However, the role of galectin-3 within the CD4+CD25+Foxp3+ T regulatory (TREG) cell compartment has not yet been explored. Here, we found, in a model of Leishmania major infection, that galectin-3 deficiency increases the frequency of peripheral TREG cells both in draining lymph nodes (LNs) and sites of infection. These observations correlated with an increased severity of the disease, as shown by increased footpad swelling and parasite burden. Galectin-3-deficient (Lgals3−/−) TREG cells displayed higher CD103 expression, showed greater suppressive capacity, and synthesized higher amounts of IL-10 compared with their wild-type (WT) counterpart. Furthermore, both TREG cells and T effector (TEFF) cells from Lgals3−/− mice showed higher expression of Notch1 and the Notch target gene Hes-1. Interestingly, Notch signaling components were also altered in both TREG and TEFF cells from uninfected Lgals3−/− mice. Thus, endogenous galectin-3 regulates the frequency and function of CD4+CD25+Foxp3+ TREG cells and alters the course of L. major infection. |
publishDate |
2013 |
dc.date.none.fl_str_mv |
2013-05-17 |
dc.type.none.fl_str_mv |
info:eu-repo/semantics/article info:eu-repo/semantics/publishedVersion http://purl.org/coar/resource_type/c_6501 info:ar-repo/semantics/articulo |
format |
article |
status_str |
publishedVersion |
dc.identifier.none.fl_str_mv |
http://hdl.handle.net/11336/2356 Fermino, Marise L.; Dias, Fabrício C.; Lopes, Carla D.; Souza, Maria A.; Cruz, Ângela K.; et al.; Galectin-3 negatively regulates the frequency and function of CD4+CD25+Foxp3+ regulatory T cells and influences the course of Leishmania major infection; Wiley VCH Verlag; European Journal of Immunology; 43; 7; 17-5-2013; 1806-1817 0014-2980 1521-4141 |
url |
http://hdl.handle.net/11336/2356 |
identifier_str_mv |
Fermino, Marise L.; Dias, Fabrício C.; Lopes, Carla D.; Souza, Maria A.; Cruz, Ângela K.; et al.; Galectin-3 negatively regulates the frequency and function of CD4+CD25+Foxp3+ regulatory T cells and influences the course of Leishmania major infection; Wiley VCH Verlag; European Journal of Immunology; 43; 7; 17-5-2013; 1806-1817 0014-2980 1521-4141 |
dc.language.none.fl_str_mv |
eng |
language |
eng |
dc.relation.none.fl_str_mv |
info:eu-repo/semantics/altIdentifier/doi/10.1002/eji.201343381 info:eu-repo/semantics/altIdentifier/url/http://onlinelibrary.wiley.com/doi/10.1002/eji.201343381/abstract;jsessionid=67113EC5897BBBED63DAF7E40BDBBE33.f03t04 |
dc.rights.none.fl_str_mv |
info:eu-repo/semantics/openAccess https://creativecommons.org/licenses/by-nc-sa/2.5/ar/ |
eu_rights_str_mv |
openAccess |
rights_invalid_str_mv |
https://creativecommons.org/licenses/by-nc-sa/2.5/ar/ |
dc.format.none.fl_str_mv |
application/pdf application/pdf application/pdf |
dc.publisher.none.fl_str_mv |
Wiley VCH Verlag |
publisher.none.fl_str_mv |
Wiley VCH Verlag |
dc.source.none.fl_str_mv |
reponame:CONICET Digital (CONICET) instname:Consejo Nacional de Investigaciones Científicas y Técnicas |
reponame_str |
CONICET Digital (CONICET) |
collection |
CONICET Digital (CONICET) |
instname_str |
Consejo Nacional de Investigaciones Científicas y Técnicas |
repository.name.fl_str_mv |
CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicas |
repository.mail.fl_str_mv |
dasensio@conicet.gov.ar; lcarlino@conicet.gov.ar |
_version_ |
1842268873468936192 |
score |
13.13397 |