Actin levels fluctuate in the seminiferous epithelium at various stages during spermatogenesis in the rat

Autores
Acosta, Cristian Gabriel; Foscolo, Mabel Rosa; Cavicchia, Juan Carlos
Año de publicación
2017
Idioma
inglés
Tipo de recurso
artículo
Estado
versión publicada
Descripción
The testis is a double gland comprised of the sperm-producing seminiferous tubules and a complex endocrine interstice. The former structures generate and release whole cells (exocrine aspect of the gland) while the latter synthesize and release androgens and related hormones. The testis also has poorly understood paracrine connexions. These connexions play an important role during spermatogenesis. A key molecule within these structures is β-actin. Therefore, the present study aims at examining the expression pattern of β-actin during the various stages of the spermatic cycle in the rat. To achieve this goal, we used a combination of trans-illumination assisted microdissection of seminiferous tubules, confocal immunofluorescence and Western blot analysis. Our experiments revealed that the levels of β-actin fluctuate significantly during spermatogenesis, peaking immediately after spermiation.
Fil: Acosta, Cristian Gabriel. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Mendoza. Instituto de Histología y Embriología de Mendoza Dr. Mario H. Burgos. Universidad Nacional de Cuyo. Facultad de Ciencias Médicas. Instituto de Histología y Embriología de Mendoza Dr. Mario H. Burgos; Argentina
Fil: Foscolo, Mabel Rosa. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Mendoza. Instituto de Histología y Embriología de Mendoza Dr. Mario H. Burgos. Universidad Nacional de Cuyo. Facultad de Ciencias Médicas. Instituto de Histología y Embriología de Mendoza Dr. Mario H. Burgos; Argentina
Fil: Cavicchia, Juan Carlos. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Mendoza. Instituto de Histología y Embriología de Mendoza Dr. Mario H. Burgos. Universidad Nacional de Cuyo. Facultad de Ciencias Médicas. Instituto de Histología y Embriología de Mendoza Dr. Mario H. Burgos; Argentina
Materia
BETA ACTIN
SEMINIFEROUS EPHITELIUM
TRANS-ILLUMINATION ASSISTED DISSECTION
Nivel de accesibilidad
acceso abierto
Condiciones de uso
https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
Repositorio
CONICET Digital (CONICET)
Institución
Consejo Nacional de Investigaciones Científicas y Técnicas
OAI Identificador
oai:ri.conicet.gov.ar:11336/81175
Acceder
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oai_identifier_str oai:ri.conicet.gov.ar:11336/81175
network_acronym_str CONICETDig
repository_id_str 3498
network_name_str CONICET Digital (CONICET)
spelling Actin levels fluctuate in the seminiferous epithelium at various stages during spermatogenesis in the ratAcosta, Cristian GabrielFoscolo, Mabel RosaCavicchia, Juan CarlosBETA ACTINSEMINIFEROUS EPHITELIUMTRANS-ILLUMINATION ASSISTED DISSECTIONhttps://purl.org/becyt/ford/1.6https://purl.org/becyt/ford/1The testis is a double gland comprised of the sperm-producing seminiferous tubules and a complex endocrine interstice. The former structures generate and release whole cells (exocrine aspect of the gland) while the latter synthesize and release androgens and related hormones. The testis also has poorly understood paracrine connexions. These connexions play an important role during spermatogenesis. A key molecule within these structures is β-actin. Therefore, the present study aims at examining the expression pattern of β-actin during the various stages of the spermatic cycle in the rat. To achieve this goal, we used a combination of trans-illumination assisted microdissection of seminiferous tubules, confocal immunofluorescence and Western blot analysis. Our experiments revealed that the levels of β-actin fluctuate significantly during spermatogenesis, peaking immediately after spermiation.Fil: Acosta, Cristian Gabriel. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Mendoza. Instituto de Histología y Embriología de Mendoza Dr. Mario H. Burgos. Universidad Nacional de Cuyo. Facultad de Ciencias Médicas. Instituto de Histología y Embriología de Mendoza Dr. Mario H. Burgos; ArgentinaFil: Foscolo, Mabel Rosa. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Mendoza. Instituto de Histología y Embriología de Mendoza Dr. Mario H. Burgos. Universidad Nacional de Cuyo. Facultad de Ciencias Médicas. Instituto de Histología y Embriología de Mendoza Dr. Mario H. Burgos; ArgentinaFil: Cavicchia, Juan Carlos. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Mendoza. Instituto de Histología y Embriología de Mendoza Dr. Mario H. Burgos. Universidad Nacional de Cuyo. Facultad de Ciencias Médicas. Instituto de Histología y Embriología de Mendoza Dr. Mario H. Burgos; ArgentinaInstituto de Histología y Embriología de Mendoza "Dr. Mario H. Burgos"2017-12info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionhttp://purl.org/coar/resource_type/c_6501info:ar-repo/semantics/articuloapplication/pdfapplication/pdfapplication/pdfhttp://hdl.handle.net/11336/81175Acosta, Cristian Gabriel; Foscolo, Mabel Rosa; Cavicchia, Juan Carlos; Actin levels fluctuate in the seminiferous epithelium at various stages during spermatogenesis in the rat; Instituto de Histología y Embriología de Mendoza "Dr. Mario H. Burgos"; Biocell; 41; 2-3; 12-2017; 63-650327-95451667-5746CONICET DigitalCONICETenginfo:eu-repo/semantics/altIdentifier/url/https://www.mendoza-conicet.gob.ar/portal/biocell/vol/pdf/41_2-3/6-MS7004%20Acosta%20FINAL.pdfinfo:eu-repo/semantics/openAccesshttps://creativecommons.org/licenses/by-nc-sa/2.5/ar/reponame:CONICET Digital (CONICET)instname:Consejo Nacional de Investigaciones Científicas y Técnicas2025-09-29T10:36:38Zoai:ri.conicet.gov.ar:11336/81175instacron:CONICETInstitucionalhttp://ri.conicet.gov.ar/Organismo científico-tecnológicoNo correspondehttp://ri.conicet.gov.ar/oai/requestdasensio@conicet.gov.ar; lcarlino@conicet.gov.arArgentinaNo correspondeNo correspondeNo correspondeopendoar:34982025-09-29 10:36:38.694CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicasfalse
dc.title.none.fl_str_mv Actin levels fluctuate in the seminiferous epithelium at various stages during spermatogenesis in the rat
title Actin levels fluctuate in the seminiferous epithelium at various stages during spermatogenesis in the rat
spellingShingle Actin levels fluctuate in the seminiferous epithelium at various stages during spermatogenesis in the rat
Acosta, Cristian Gabriel
BETA ACTIN
SEMINIFEROUS EPHITELIUM
TRANS-ILLUMINATION ASSISTED DISSECTION
title_short Actin levels fluctuate in the seminiferous epithelium at various stages during spermatogenesis in the rat
title_full Actin levels fluctuate in the seminiferous epithelium at various stages during spermatogenesis in the rat
title_fullStr Actin levels fluctuate in the seminiferous epithelium at various stages during spermatogenesis in the rat
title_full_unstemmed Actin levels fluctuate in the seminiferous epithelium at various stages during spermatogenesis in the rat
title_sort Actin levels fluctuate in the seminiferous epithelium at various stages during spermatogenesis in the rat
dc.creator.none.fl_str_mv Acosta, Cristian Gabriel
Foscolo, Mabel Rosa
Cavicchia, Juan Carlos
author Acosta, Cristian Gabriel
author_facet Acosta, Cristian Gabriel
Foscolo, Mabel Rosa
Cavicchia, Juan Carlos
author_role author
author2 Foscolo, Mabel Rosa
Cavicchia, Juan Carlos
author2_role author
author
dc.subject.none.fl_str_mv BETA ACTIN
SEMINIFEROUS EPHITELIUM
TRANS-ILLUMINATION ASSISTED DISSECTION
topic BETA ACTIN
SEMINIFEROUS EPHITELIUM
TRANS-ILLUMINATION ASSISTED DISSECTION
purl_subject.fl_str_mv https://purl.org/becyt/ford/1.6
https://purl.org/becyt/ford/1
dc.description.none.fl_txt_mv The testis is a double gland comprised of the sperm-producing seminiferous tubules and a complex endocrine interstice. The former structures generate and release whole cells (exocrine aspect of the gland) while the latter synthesize and release androgens and related hormones. The testis also has poorly understood paracrine connexions. These connexions play an important role during spermatogenesis. A key molecule within these structures is β-actin. Therefore, the present study aims at examining the expression pattern of β-actin during the various stages of the spermatic cycle in the rat. To achieve this goal, we used a combination of trans-illumination assisted microdissection of seminiferous tubules, confocal immunofluorescence and Western blot analysis. Our experiments revealed that the levels of β-actin fluctuate significantly during spermatogenesis, peaking immediately after spermiation.
Fil: Acosta, Cristian Gabriel. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Mendoza. Instituto de Histología y Embriología de Mendoza Dr. Mario H. Burgos. Universidad Nacional de Cuyo. Facultad de Ciencias Médicas. Instituto de Histología y Embriología de Mendoza Dr. Mario H. Burgos; Argentina
Fil: Foscolo, Mabel Rosa. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Mendoza. Instituto de Histología y Embriología de Mendoza Dr. Mario H. Burgos. Universidad Nacional de Cuyo. Facultad de Ciencias Médicas. Instituto de Histología y Embriología de Mendoza Dr. Mario H. Burgos; Argentina
Fil: Cavicchia, Juan Carlos. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Mendoza. Instituto de Histología y Embriología de Mendoza Dr. Mario H. Burgos. Universidad Nacional de Cuyo. Facultad de Ciencias Médicas. Instituto de Histología y Embriología de Mendoza Dr. Mario H. Burgos; Argentina
description The testis is a double gland comprised of the sperm-producing seminiferous tubules and a complex endocrine interstice. The former structures generate and release whole cells (exocrine aspect of the gland) while the latter synthesize and release androgens and related hormones. The testis also has poorly understood paracrine connexions. These connexions play an important role during spermatogenesis. A key molecule within these structures is β-actin. Therefore, the present study aims at examining the expression pattern of β-actin during the various stages of the spermatic cycle in the rat. To achieve this goal, we used a combination of trans-illumination assisted microdissection of seminiferous tubules, confocal immunofluorescence and Western blot analysis. Our experiments revealed that the levels of β-actin fluctuate significantly during spermatogenesis, peaking immediately after spermiation.
publishDate 2017
dc.date.none.fl_str_mv 2017-12
dc.type.none.fl_str_mv info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
http://purl.org/coar/resource_type/c_6501
info:ar-repo/semantics/articulo
format article
status_str publishedVersion
dc.identifier.none.fl_str_mv http://hdl.handle.net/11336/81175
Acosta, Cristian Gabriel; Foscolo, Mabel Rosa; Cavicchia, Juan Carlos; Actin levels fluctuate in the seminiferous epithelium at various stages during spermatogenesis in the rat; Instituto de Histología y Embriología de Mendoza "Dr. Mario H. Burgos"; Biocell; 41; 2-3; 12-2017; 63-65
0327-9545
1667-5746
CONICET Digital
CONICET
url http://hdl.handle.net/11336/81175
identifier_str_mv Acosta, Cristian Gabriel; Foscolo, Mabel Rosa; Cavicchia, Juan Carlos; Actin levels fluctuate in the seminiferous epithelium at various stages during spermatogenesis in the rat; Instituto de Histología y Embriología de Mendoza "Dr. Mario H. Burgos"; Biocell; 41; 2-3; 12-2017; 63-65
0327-9545
1667-5746
CONICET Digital
CONICET
dc.language.none.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv info:eu-repo/semantics/altIdentifier/url/https://www.mendoza-conicet.gob.ar/portal/biocell/vol/pdf/41_2-3/6-MS7004%20Acosta%20FINAL.pdf
dc.rights.none.fl_str_mv info:eu-repo/semantics/openAccess
https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
eu_rights_str_mv openAccess
rights_invalid_str_mv https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
dc.format.none.fl_str_mv application/pdf
application/pdf
application/pdf
dc.publisher.none.fl_str_mv Instituto de Histología y Embriología de Mendoza "Dr. Mario H. Burgos"
publisher.none.fl_str_mv Instituto de Histología y Embriología de Mendoza "Dr. Mario H. Burgos"
dc.source.none.fl_str_mv reponame:CONICET Digital (CONICET)
instname:Consejo Nacional de Investigaciones Científicas y Técnicas
reponame_str CONICET Digital (CONICET)
collection CONICET Digital (CONICET)
instname_str Consejo Nacional de Investigaciones Científicas y Técnicas
repository.name.fl_str_mv CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicas
repository.mail.fl_str_mv dasensio@conicet.gov.ar; lcarlino@conicet.gov.ar
_version_ 1844614387401228288
score 13.070432

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