Redirection of the immune response to the functional catalytic domain of cruzipain improves protective immunity against Trypanosoma cruzi infection

Autores
Cazorla, Silvia Ines; Frank, Fernanda Maria; Becker, Pablo D.; Arnaiz, María; Mirkin, Gerardo Ariel Isidoro; Corral, Ricardo Santiago; Guzman, Carlos Alberto; Malchiodi, Emilio Luis
Año de publicación
2010
Idioma
inglés
Tipo de recurso
artículo
Estado
versión publicada
Descripción
Despite the strong immune responses elicited after natural infection with Trypanosoma cruzi or vaccination against it, parasite survival suggests that these responses are insufficient or inherently inadequate. T. cruzi contains a major cystein proteinase, cruzipain, which has a catalytic N-terminal domain and a C-terminal extension. Immunizations that employed recombinant cruzipain or its N- and C-terminal domains allowed evaluation of the ability of cruzipain to circumvent responses against the catalytic domain. This phenomenon is not a property of the parasite but of cruzipain itself, because recombinant cruzipain triggers a response similar to that of cruzipain during natural or experimental infection. Cruzipain is not the only antigen with a highly immunogenic region of unknown function that somehow protects an essential domain for parasite survival. However, our studies show that this can be reverted by using the N-terminal domain as a tailored immunogen able to redirect host responses to provide enhanced protection.
Fil: Cazorla, Silvia Ines. Consejo Nacional de Investigaciones Cientificas y Tecnicas. Oficina de Coordinacion Administrativa Houssay. Instituto de Estudios de la Inmunidad Humoral "Profesor R. A. Margni"; Argentina. Universidad de Buenos Aires. Facultad de Farmacia y Bioquímica; Argentina. Universidad de Buenos Aires. Facultad de Medicina; Argentina. Helmholtz Centre for Infection Research; Alemania
Fil: Frank, Fernanda Maria. Consejo Nacional de Investigaciones Cientificas y Tecnicas. Oficina de Coordinacion Administrativa Houssay. Instituto de Estudios de la Inmunidad Humoral "Profesor R. A. Margni"; Argentina. Universidad de Buenos Aires. Facultad de Medicina; Argentina
Fil: Becker, Pablo D.. Helmholtz Centre for Infection Research; Alemania
Fil: Arnaiz, María. Dirección Nacional de Instituto de Investigación. Administración Nacional de Laboratorio e Instituto de Salud; Argentina
Fil: Mirkin, Gerardo Ariel Isidoro. Universidad de Buenos Aires. Facultad de Medicina; Argentina
Fil: Corral, Ricardo Santiago. Gobierno de la Ciudad de Buenos Aires. Hospital General de Niños "Ricardo Gutierrez"; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina
Fil: Guzman, Carlos Alberto. Helmholtz Centre for Infection Research; Alemania. Consejo Nacional de Investigaciones Cientificas y Tecnicas. Oficina de Coordinacion Administrativa Houssay. Instituto de Estudios de la Inmunidad Humoral "Profesor R. A. Margni"; Argentina
Fil: Malchiodi, Emilio Luis. Consejo Nacional de Investigaciones Cientificas y Tecnicas. Oficina de Coordinacion Administrativa Houssay. Instituto de Estudios de la Inmunidad Humoral "Profesor R. A. Margni"; Argentina. Universidad de Buenos Aires. Facultad de Farmacia y Bioquímica; Argentina. Universidad de Buenos Aires. Facultad de Medicina; Argentina
Materia
Chagas Disease
Immune Response
Antigens
Catalysis
Catalytic Domain
Endopeptidases
Immunity
Immunization
Parasites
Precipitating Factors
Trypanosoma Cruzi
Vaccination
Vaccines
Infection
Mice
Proteolytic Enzymes
Nivel de accesibilidad
acceso abierto
Condiciones de uso
https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
Repositorio
CONICET Digital (CONICET)
Institución
Consejo Nacional de Investigaciones Científicas y Técnicas
OAI Identificador
oai:ri.conicet.gov.ar:11336/13925
Acceder
id CONICETDig_16bf8a00464f780d46796b4f0b8adc63
oai_identifier_str oai:ri.conicet.gov.ar:11336/13925
network_acronym_str CONICETDig
repository_id_str 3498
network_name_str CONICET Digital (CONICET)
spelling Redirection of the immune response to the functional catalytic domain of cruzipain improves protective immunity against Trypanosoma cruzi infectionCazorla, Silvia InesFrank, Fernanda MariaBecker, Pablo D.Arnaiz, MaríaMirkin, Gerardo Ariel IsidoroCorral, Ricardo SantiagoGuzman, Carlos AlbertoMalchiodi, Emilio LuisChagas DiseaseImmune ResponseAntigensCatalysisCatalytic DomainEndopeptidasesImmunityImmunizationParasitesPrecipitating FactorsTrypanosoma CruziVaccinationVaccinesInfectionMiceProteolytic Enzymeshttps://purl.org/becyt/ford/3.1https://purl.org/becyt/ford/3Despite the strong immune responses elicited after natural infection with Trypanosoma cruzi or vaccination against it, parasite survival suggests that these responses are insufficient or inherently inadequate. T. cruzi contains a major cystein proteinase, cruzipain, which has a catalytic N-terminal domain and a C-terminal extension. Immunizations that employed recombinant cruzipain or its N- and C-terminal domains allowed evaluation of the ability of cruzipain to circumvent responses against the catalytic domain. This phenomenon is not a property of the parasite but of cruzipain itself, because recombinant cruzipain triggers a response similar to that of cruzipain during natural or experimental infection. Cruzipain is not the only antigen with a highly immunogenic region of unknown function that somehow protects an essential domain for parasite survival. However, our studies show that this can be reverted by using the N-terminal domain as a tailored immunogen able to redirect host responses to provide enhanced protection.Fil: Cazorla, Silvia Ines. Consejo Nacional de Investigaciones Cientificas y Tecnicas. Oficina de Coordinacion Administrativa Houssay. Instituto de Estudios de la Inmunidad Humoral "Profesor R. A. Margni"; Argentina. Universidad de Buenos Aires. Facultad de Farmacia y Bioquímica; Argentina. Universidad de Buenos Aires. Facultad de Medicina; Argentina. Helmholtz Centre for Infection Research; AlemaniaFil: Frank, Fernanda Maria. Consejo Nacional de Investigaciones Cientificas y Tecnicas. Oficina de Coordinacion Administrativa Houssay. Instituto de Estudios de la Inmunidad Humoral "Profesor R. A. Margni"; Argentina. Universidad de Buenos Aires. Facultad de Medicina; ArgentinaFil: Becker, Pablo D.. Helmholtz Centre for Infection Research; AlemaniaFil: Arnaiz, María. Dirección Nacional de Instituto de Investigación. Administración Nacional de Laboratorio e Instituto de Salud; ArgentinaFil: Mirkin, Gerardo Ariel Isidoro. Universidad de Buenos Aires. Facultad de Medicina; ArgentinaFil: Corral, Ricardo Santiago. Gobierno de la Ciudad de Buenos Aires. Hospital General de Niños "Ricardo Gutierrez"; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; ArgentinaFil: Guzman, Carlos Alberto. Helmholtz Centre for Infection Research; Alemania. Consejo Nacional de Investigaciones Cientificas y Tecnicas. Oficina de Coordinacion Administrativa Houssay. Instituto de Estudios de la Inmunidad Humoral "Profesor R. A. Margni"; ArgentinaFil: Malchiodi, Emilio Luis. Consejo Nacional de Investigaciones Cientificas y Tecnicas. Oficina de Coordinacion Administrativa Houssay. Instituto de Estudios de la Inmunidad Humoral "Profesor R. A. Margni"; Argentina. Universidad de Buenos Aires. Facultad de Farmacia y Bioquímica; Argentina. Universidad de Buenos Aires. Facultad de Medicina; ArgentinaOxford University Press2010-12info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionhttp://purl.org/coar/resource_type/c_6501info:ar-repo/semantics/articuloapplication/pdfapplication/pdfapplication/pdfapplication/pdfhttp://hdl.handle.net/11336/13925Cazorla, Silvia Ines; Frank, Fernanda Maria; Becker, Pablo D.; Arnaiz, María; Mirkin, Gerardo Ariel Isidoro; et al.; Redirection of the immune response to the functional catalytic domain of cruzipain improves protective immunity against Trypanosoma cruzi infection; Oxford University Press; Journal Of Infectious Diseases; 202; 12; 12-2010; 136-1440022-1899enginfo:eu-repo/semantics/altIdentifier/url/https://academic.oup.com/jid/article-lookup/doi/10.1086/652872info:eu-repo/semantics/altIdentifier/doi/10.1086/652872info:eu-repo/semantics/openAccesshttps://creativecommons.org/licenses/by-nc-sa/2.5/ar/reponame:CONICET Digital (CONICET)instname:Consejo Nacional de Investigaciones Científicas y Técnicas2025-09-29T10:33:07Zoai:ri.conicet.gov.ar:11336/13925instacron:CONICETInstitucionalhttp://ri.conicet.gov.ar/Organismo científico-tecnológicoNo correspondehttp://ri.conicet.gov.ar/oai/requestdasensio@conicet.gov.ar; lcarlino@conicet.gov.arArgentinaNo correspondeNo correspondeNo correspondeopendoar:34982025-09-29 10:33:07.991CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicasfalse
dc.title.none.fl_str_mv Redirection of the immune response to the functional catalytic domain of cruzipain improves protective immunity against Trypanosoma cruzi infection
title Redirection of the immune response to the functional catalytic domain of cruzipain improves protective immunity against Trypanosoma cruzi infection
spellingShingle Redirection of the immune response to the functional catalytic domain of cruzipain improves protective immunity against Trypanosoma cruzi infection
Cazorla, Silvia Ines
Chagas Disease
Immune Response
Antigens
Catalysis
Catalytic Domain
Endopeptidases
Immunity
Immunization
Parasites
Precipitating Factors
Trypanosoma Cruzi
Vaccination
Vaccines
Infection
Mice
Proteolytic Enzymes
title_short Redirection of the immune response to the functional catalytic domain of cruzipain improves protective immunity against Trypanosoma cruzi infection
title_full Redirection of the immune response to the functional catalytic domain of cruzipain improves protective immunity against Trypanosoma cruzi infection
title_fullStr Redirection of the immune response to the functional catalytic domain of cruzipain improves protective immunity against Trypanosoma cruzi infection
title_full_unstemmed Redirection of the immune response to the functional catalytic domain of cruzipain improves protective immunity against Trypanosoma cruzi infection
title_sort Redirection of the immune response to the functional catalytic domain of cruzipain improves protective immunity against Trypanosoma cruzi infection
dc.creator.none.fl_str_mv Cazorla, Silvia Ines
Frank, Fernanda Maria
Becker, Pablo D.
Arnaiz, María
Mirkin, Gerardo Ariel Isidoro
Corral, Ricardo Santiago
Guzman, Carlos Alberto
Malchiodi, Emilio Luis
author Cazorla, Silvia Ines
author_facet Cazorla, Silvia Ines
Frank, Fernanda Maria
Becker, Pablo D.
Arnaiz, María
Mirkin, Gerardo Ariel Isidoro
Corral, Ricardo Santiago
Guzman, Carlos Alberto
Malchiodi, Emilio Luis
author_role author
author2 Frank, Fernanda Maria
Becker, Pablo D.
Arnaiz, María
Mirkin, Gerardo Ariel Isidoro
Corral, Ricardo Santiago
Guzman, Carlos Alberto
Malchiodi, Emilio Luis
author2_role author
author
author
author
author
author
author
dc.subject.none.fl_str_mv Chagas Disease
Immune Response
Antigens
Catalysis
Catalytic Domain
Endopeptidases
Immunity
Immunization
Parasites
Precipitating Factors
Trypanosoma Cruzi
Vaccination
Vaccines
Infection
Mice
Proteolytic Enzymes
topic Chagas Disease
Immune Response
Antigens
Catalysis
Catalytic Domain
Endopeptidases
Immunity
Immunization
Parasites
Precipitating Factors
Trypanosoma Cruzi
Vaccination
Vaccines
Infection
Mice
Proteolytic Enzymes
purl_subject.fl_str_mv https://purl.org/becyt/ford/3.1
https://purl.org/becyt/ford/3
dc.description.none.fl_txt_mv Despite the strong immune responses elicited after natural infection with Trypanosoma cruzi or vaccination against it, parasite survival suggests that these responses are insufficient or inherently inadequate. T. cruzi contains a major cystein proteinase, cruzipain, which has a catalytic N-terminal domain and a C-terminal extension. Immunizations that employed recombinant cruzipain or its N- and C-terminal domains allowed evaluation of the ability of cruzipain to circumvent responses against the catalytic domain. This phenomenon is not a property of the parasite but of cruzipain itself, because recombinant cruzipain triggers a response similar to that of cruzipain during natural or experimental infection. Cruzipain is not the only antigen with a highly immunogenic region of unknown function that somehow protects an essential domain for parasite survival. However, our studies show that this can be reverted by using the N-terminal domain as a tailored immunogen able to redirect host responses to provide enhanced protection.
Fil: Cazorla, Silvia Ines. Consejo Nacional de Investigaciones Cientificas y Tecnicas. Oficina de Coordinacion Administrativa Houssay. Instituto de Estudios de la Inmunidad Humoral "Profesor R. A. Margni"; Argentina. Universidad de Buenos Aires. Facultad de Farmacia y Bioquímica; Argentina. Universidad de Buenos Aires. Facultad de Medicina; Argentina. Helmholtz Centre for Infection Research; Alemania
Fil: Frank, Fernanda Maria. Consejo Nacional de Investigaciones Cientificas y Tecnicas. Oficina de Coordinacion Administrativa Houssay. Instituto de Estudios de la Inmunidad Humoral "Profesor R. A. Margni"; Argentina. Universidad de Buenos Aires. Facultad de Medicina; Argentina
Fil: Becker, Pablo D.. Helmholtz Centre for Infection Research; Alemania
Fil: Arnaiz, María. Dirección Nacional de Instituto de Investigación. Administración Nacional de Laboratorio e Instituto de Salud; Argentina
Fil: Mirkin, Gerardo Ariel Isidoro. Universidad de Buenos Aires. Facultad de Medicina; Argentina
Fil: Corral, Ricardo Santiago. Gobierno de la Ciudad de Buenos Aires. Hospital General de Niños "Ricardo Gutierrez"; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina
Fil: Guzman, Carlos Alberto. Helmholtz Centre for Infection Research; Alemania. Consejo Nacional de Investigaciones Cientificas y Tecnicas. Oficina de Coordinacion Administrativa Houssay. Instituto de Estudios de la Inmunidad Humoral "Profesor R. A. Margni"; Argentina
Fil: Malchiodi, Emilio Luis. Consejo Nacional de Investigaciones Cientificas y Tecnicas. Oficina de Coordinacion Administrativa Houssay. Instituto de Estudios de la Inmunidad Humoral "Profesor R. A. Margni"; Argentina. Universidad de Buenos Aires. Facultad de Farmacia y Bioquímica; Argentina. Universidad de Buenos Aires. Facultad de Medicina; Argentina
description Despite the strong immune responses elicited after natural infection with Trypanosoma cruzi or vaccination against it, parasite survival suggests that these responses are insufficient or inherently inadequate. T. cruzi contains a major cystein proteinase, cruzipain, which has a catalytic N-terminal domain and a C-terminal extension. Immunizations that employed recombinant cruzipain or its N- and C-terminal domains allowed evaluation of the ability of cruzipain to circumvent responses against the catalytic domain. This phenomenon is not a property of the parasite but of cruzipain itself, because recombinant cruzipain triggers a response similar to that of cruzipain during natural or experimental infection. Cruzipain is not the only antigen with a highly immunogenic region of unknown function that somehow protects an essential domain for parasite survival. However, our studies show that this can be reverted by using the N-terminal domain as a tailored immunogen able to redirect host responses to provide enhanced protection.
publishDate 2010
dc.date.none.fl_str_mv 2010-12
dc.type.none.fl_str_mv info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
http://purl.org/coar/resource_type/c_6501
info:ar-repo/semantics/articulo
format article
status_str publishedVersion
dc.identifier.none.fl_str_mv http://hdl.handle.net/11336/13925
Cazorla, Silvia Ines; Frank, Fernanda Maria; Becker, Pablo D.; Arnaiz, María; Mirkin, Gerardo Ariel Isidoro; et al.; Redirection of the immune response to the functional catalytic domain of cruzipain improves protective immunity against Trypanosoma cruzi infection; Oxford University Press; Journal Of Infectious Diseases; 202; 12; 12-2010; 136-144
0022-1899
url http://hdl.handle.net/11336/13925
identifier_str_mv Cazorla, Silvia Ines; Frank, Fernanda Maria; Becker, Pablo D.; Arnaiz, María; Mirkin, Gerardo Ariel Isidoro; et al.; Redirection of the immune response to the functional catalytic domain of cruzipain improves protective immunity against Trypanosoma cruzi infection; Oxford University Press; Journal Of Infectious Diseases; 202; 12; 12-2010; 136-144
0022-1899
dc.language.none.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv info:eu-repo/semantics/altIdentifier/url/https://academic.oup.com/jid/article-lookup/doi/10.1086/652872
info:eu-repo/semantics/altIdentifier/doi/10.1086/652872
dc.rights.none.fl_str_mv info:eu-repo/semantics/openAccess
https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
eu_rights_str_mv openAccess
rights_invalid_str_mv https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
dc.format.none.fl_str_mv application/pdf
application/pdf
application/pdf
application/pdf
dc.publisher.none.fl_str_mv Oxford University Press
publisher.none.fl_str_mv Oxford University Press
dc.source.none.fl_str_mv reponame:CONICET Digital (CONICET)
instname:Consejo Nacional de Investigaciones Científicas y Técnicas
reponame_str CONICET Digital (CONICET)
collection CONICET Digital (CONICET)
instname_str Consejo Nacional de Investigaciones Científicas y Técnicas
repository.name.fl_str_mv CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicas
repository.mail.fl_str_mv dasensio@conicet.gov.ar; lcarlino@conicet.gov.ar
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score 13.069144

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