Inmunolocalization of certain detoxifying proteins in Fasciola hepatica: relationship with resistance mechanisms

Autores
Scarcella, Silvana Andrea; Solana, Hugo Daniel; Larsen, Matías José; Felipe, Antonio Eduardo; Alzola, Ricardo; Lanusse, Carlos Edmundo
Año de publicación
2008
Idioma
inglés
Tipo de recurso
documento de conferencia
Estado
versión publicada
Descripción
Fasciolasis, is an emergent zoonotic disease. The drug of choice for its treatment is triclabendazole (TCBZ). Today, the extensive use of TCBZ has already generated resistance. The mechanisms of anthelmintic resistance include involvement of detoxification processes generated through the FMO enzymatic pathway or through drug extrusion pumps such as the P Glycoprotein (P Gp). The aim of the present work was to immune-locate the presence and distribution of P-Gp and FMO in TCBZ susceptible and resistant Fasciola hepatica. The immunohistochemical study confirmed the presence of P Gp in both strains, distributed in the enterocytes and a smaller quantity in the vitellogenic cells and specula. FMO location seems to be restricted to the vitellogenic cells. This distribution pattern was similar for both strains, without differences in their distribution and expression. It is inferred that the resistance may involve modifications at metabolic levels, without detectable morphologic changes, at least not observable by optical microscopy.
Fil: Scarcella, Silvana Andrea. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina. Universidad Nacional del Centro de la Provincia de Buenos Aires. Facultad de Ciencias Veterinarias. Departamento de Ciencias Biológicas; Argentina
Fil: Solana, Hugo Daniel. Universidad Nacional del Centro de la Provincia de Buenos Aires. Facultad de Ciencias Veterinarias. Departamento de Ciencias Biológicas; Argentina
Fil: Larsen, Matías José. Universidad Nacional del Centro de la Provincia de Buenos Aires. Facultad de Ciencias Veterinarias. Departamento de Ciencias Biológicas; Argentina
Fil: Felipe, Antonio Eduardo. Universidad Nacional del Centro de la Provincia de Buenos Aires. Facultad de Ciencias Veterinarias. Departamento de Ciencias Biológicas; Argentina
Fil: Alzola, Ricardo. Universidad Nacional del Centro de la Provincia de Buenos Aires. Facultad de Ciencias Veterinarias. Departamento de Ciencias Biológicas; Argentina
Fil: Lanusse, Carlos Edmundo. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina. Universidad Nacional del Centro de la Provincia de Buenos Aires. Facultad de Ciencias Veterinarias. Departamento de Fisiopatología. Laboratorio de Farmacología; Argentina
XL Reunión Anual de la Sociedad Argentina de Farmacología Experimental
Tandil
Argentina
Sociedad Argentina de Farmacologia Experimental
Universidad Nacional del Centro de la Provincia de Buenos Aires. Facultad de Ciencias Veterinarias
Materia
Inmunolocalization
Fasciola
Detoxifying
Nivel de accesibilidad
acceso abierto
Condiciones de uso
https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
Repositorio
CONICET Digital (CONICET)
Institución
Consejo Nacional de Investigaciones Científicas y Técnicas
OAI Identificador
oai:ri.conicet.gov.ar:11336/269167
Acceder
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oai_identifier_str oai:ri.conicet.gov.ar:11336/269167
network_acronym_str CONICETDig
repository_id_str 3498
network_name_str CONICET Digital (CONICET)
spelling Inmunolocalization of certain detoxifying proteins in Fasciola hepatica: relationship with resistance mechanismsScarcella, Silvana AndreaSolana, Hugo DanielLarsen, Matías JoséFelipe, Antonio EduardoAlzola, RicardoLanusse, Carlos EdmundoInmunolocalizationFasciolaDetoxifyinghttps://purl.org/becyt/ford/4.3https://purl.org/becyt/ford/4Fasciolasis, is an emergent zoonotic disease. The drug of choice for its treatment is triclabendazole (TCBZ). Today, the extensive use of TCBZ has already generated resistance. The mechanisms of anthelmintic resistance include involvement of detoxification processes generated through the FMO enzymatic pathway or through drug extrusion pumps such as the P Glycoprotein (P Gp). The aim of the present work was to immune-locate the presence and distribution of P-Gp and FMO in TCBZ susceptible and resistant Fasciola hepatica. The immunohistochemical study confirmed the presence of P Gp in both strains, distributed in the enterocytes and a smaller quantity in the vitellogenic cells and specula. FMO location seems to be restricted to the vitellogenic cells. This distribution pattern was similar for both strains, without differences in their distribution and expression. It is inferred that the resistance may involve modifications at metabolic levels, without detectable morphologic changes, at least not observable by optical microscopy.Fil: Scarcella, Silvana Andrea. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina. Universidad Nacional del Centro de la Provincia de Buenos Aires. Facultad de Ciencias Veterinarias. Departamento de Ciencias Biológicas; ArgentinaFil: Solana, Hugo Daniel. Universidad Nacional del Centro de la Provincia de Buenos Aires. Facultad de Ciencias Veterinarias. Departamento de Ciencias Biológicas; ArgentinaFil: Larsen, Matías José. Universidad Nacional del Centro de la Provincia de Buenos Aires. Facultad de Ciencias Veterinarias. Departamento de Ciencias Biológicas; ArgentinaFil: Felipe, Antonio Eduardo. Universidad Nacional del Centro de la Provincia de Buenos Aires. Facultad de Ciencias Veterinarias. Departamento de Ciencias Biológicas; ArgentinaFil: Alzola, Ricardo. Universidad Nacional del Centro de la Provincia de Buenos Aires. Facultad de Ciencias Veterinarias. Departamento de Ciencias Biológicas; ArgentinaFil: Lanusse, Carlos Edmundo. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina. Universidad Nacional del Centro de la Provincia de Buenos Aires. Facultad de Ciencias Veterinarias. Departamento de Fisiopatología. Laboratorio de Farmacología; ArgentinaXL Reunión Anual de la Sociedad Argentina de Farmacología ExperimentalTandilArgentinaSociedad Argentina de Farmacologia ExperimentalUniversidad Nacional del Centro de la Provincia de Buenos Aires. Facultad de Ciencias VeterinariasSociedad Argentina de Farmacologia Experimental2008info:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/conferenceObjectReuniónBookhttp://purl.org/coar/resource_type/c_5794info:ar-repo/semantics/documentoDeConferenciaapplication/pdfapplication/mswordapplication/pdfhttp://hdl.handle.net/11336/269167Inmunolocalization of certain detoxifying proteins in Fasciola hepatica: relationship with resistance mechanisms; XL Reunión Anual de la Sociedad Argentina de Farmacología Experimental; Tandil; Argentina; 2008; 20-20CONICET DigitalCONICETenginfo:eu-repo/semantics/altIdentifier/url/https://aafeargentina.org/congresos-aafe/info:eu-repo/semantics/openAccesshttps://creativecommons.org/licenses/by-nc-sa/2.5/ar/reponame:CONICET Digital (CONICET)instname:Consejo Nacional de Investigaciones Científicas y Técnicas2025-09-29T09:42:20Zoai:ri.conicet.gov.ar:11336/269167instacron:CONICETInstitucionalhttp://ri.conicet.gov.ar/Organismo científico-tecnológicoNo correspondehttp://ri.conicet.gov.ar/oai/requestdasensio@conicet.gov.ar; lcarlino@conicet.gov.arArgentinaNo correspondeNo correspondeNo correspondeopendoar:34982025-09-29 09:42:20.358CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicasfalse
dc.title.none.fl_str_mv Inmunolocalization of certain detoxifying proteins in Fasciola hepatica: relationship with resistance mechanisms
title Inmunolocalization of certain detoxifying proteins in Fasciola hepatica: relationship with resistance mechanisms
spellingShingle Inmunolocalization of certain detoxifying proteins in Fasciola hepatica: relationship with resistance mechanisms
Scarcella, Silvana Andrea
Inmunolocalization
Fasciola
Detoxifying
title_short Inmunolocalization of certain detoxifying proteins in Fasciola hepatica: relationship with resistance mechanisms
title_full Inmunolocalization of certain detoxifying proteins in Fasciola hepatica: relationship with resistance mechanisms
title_fullStr Inmunolocalization of certain detoxifying proteins in Fasciola hepatica: relationship with resistance mechanisms
title_full_unstemmed Inmunolocalization of certain detoxifying proteins in Fasciola hepatica: relationship with resistance mechanisms
title_sort Inmunolocalization of certain detoxifying proteins in Fasciola hepatica: relationship with resistance mechanisms
dc.creator.none.fl_str_mv Scarcella, Silvana Andrea
Solana, Hugo Daniel
Larsen, Matías José
Felipe, Antonio Eduardo
Alzola, Ricardo
Lanusse, Carlos Edmundo
author Scarcella, Silvana Andrea
author_facet Scarcella, Silvana Andrea
Solana, Hugo Daniel
Larsen, Matías José
Felipe, Antonio Eduardo
Alzola, Ricardo
Lanusse, Carlos Edmundo
author_role author
author2 Solana, Hugo Daniel
Larsen, Matías José
Felipe, Antonio Eduardo
Alzola, Ricardo
Lanusse, Carlos Edmundo
author2_role author
author
author
author
author
dc.subject.none.fl_str_mv Inmunolocalization
Fasciola
Detoxifying
topic Inmunolocalization
Fasciola
Detoxifying
purl_subject.fl_str_mv https://purl.org/becyt/ford/4.3
https://purl.org/becyt/ford/4
dc.description.none.fl_txt_mv Fasciolasis, is an emergent zoonotic disease. The drug of choice for its treatment is triclabendazole (TCBZ). Today, the extensive use of TCBZ has already generated resistance. The mechanisms of anthelmintic resistance include involvement of detoxification processes generated through the FMO enzymatic pathway or through drug extrusion pumps such as the P Glycoprotein (P Gp). The aim of the present work was to immune-locate the presence and distribution of P-Gp and FMO in TCBZ susceptible and resistant Fasciola hepatica. The immunohistochemical study confirmed the presence of P Gp in both strains, distributed in the enterocytes and a smaller quantity in the vitellogenic cells and specula. FMO location seems to be restricted to the vitellogenic cells. This distribution pattern was similar for both strains, without differences in their distribution and expression. It is inferred that the resistance may involve modifications at metabolic levels, without detectable morphologic changes, at least not observable by optical microscopy.
Fil: Scarcella, Silvana Andrea. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina. Universidad Nacional del Centro de la Provincia de Buenos Aires. Facultad de Ciencias Veterinarias. Departamento de Ciencias Biológicas; Argentina
Fil: Solana, Hugo Daniel. Universidad Nacional del Centro de la Provincia de Buenos Aires. Facultad de Ciencias Veterinarias. Departamento de Ciencias Biológicas; Argentina
Fil: Larsen, Matías José. Universidad Nacional del Centro de la Provincia de Buenos Aires. Facultad de Ciencias Veterinarias. Departamento de Ciencias Biológicas; Argentina
Fil: Felipe, Antonio Eduardo. Universidad Nacional del Centro de la Provincia de Buenos Aires. Facultad de Ciencias Veterinarias. Departamento de Ciencias Biológicas; Argentina
Fil: Alzola, Ricardo. Universidad Nacional del Centro de la Provincia de Buenos Aires. Facultad de Ciencias Veterinarias. Departamento de Ciencias Biológicas; Argentina
Fil: Lanusse, Carlos Edmundo. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina. Universidad Nacional del Centro de la Provincia de Buenos Aires. Facultad de Ciencias Veterinarias. Departamento de Fisiopatología. Laboratorio de Farmacología; Argentina
XL Reunión Anual de la Sociedad Argentina de Farmacología Experimental
Tandil
Argentina
Sociedad Argentina de Farmacologia Experimental
Universidad Nacional del Centro de la Provincia de Buenos Aires. Facultad de Ciencias Veterinarias
description Fasciolasis, is an emergent zoonotic disease. The drug of choice for its treatment is triclabendazole (TCBZ). Today, the extensive use of TCBZ has already generated resistance. The mechanisms of anthelmintic resistance include involvement of detoxification processes generated through the FMO enzymatic pathway or through drug extrusion pumps such as the P Glycoprotein (P Gp). The aim of the present work was to immune-locate the presence and distribution of P-Gp and FMO in TCBZ susceptible and resistant Fasciola hepatica. The immunohistochemical study confirmed the presence of P Gp in both strains, distributed in the enterocytes and a smaller quantity in the vitellogenic cells and specula. FMO location seems to be restricted to the vitellogenic cells. This distribution pattern was similar for both strains, without differences in their distribution and expression. It is inferred that the resistance may involve modifications at metabolic levels, without detectable morphologic changes, at least not observable by optical microscopy.
publishDate 2008
dc.date.none.fl_str_mv 2008
dc.type.none.fl_str_mv info:eu-repo/semantics/publishedVersion
info:eu-repo/semantics/conferenceObject
Reunión
Book
http://purl.org/coar/resource_type/c_5794
info:ar-repo/semantics/documentoDeConferencia
status_str publishedVersion
format conferenceObject
dc.identifier.none.fl_str_mv http://hdl.handle.net/11336/269167
Inmunolocalization of certain detoxifying proteins in Fasciola hepatica: relationship with resistance mechanisms; XL Reunión Anual de la Sociedad Argentina de Farmacología Experimental; Tandil; Argentina; 2008; 20-20
CONICET Digital
CONICET
url http://hdl.handle.net/11336/269167
identifier_str_mv Inmunolocalization of certain detoxifying proteins in Fasciola hepatica: relationship with resistance mechanisms; XL Reunión Anual de la Sociedad Argentina de Farmacología Experimental; Tandil; Argentina; 2008; 20-20
CONICET Digital
CONICET
dc.language.none.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv info:eu-repo/semantics/altIdentifier/url/https://aafeargentina.org/congresos-aafe/
dc.rights.none.fl_str_mv info:eu-repo/semantics/openAccess
https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
eu_rights_str_mv openAccess
rights_invalid_str_mv https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
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application/msword
application/pdf
dc.publisher.none.fl_str_mv Sociedad Argentina de Farmacologia Experimental
publisher.none.fl_str_mv Sociedad Argentina de Farmacologia Experimental
dc.source.none.fl_str_mv reponame:CONICET Digital (CONICET)
instname:Consejo Nacional de Investigaciones Científicas y Técnicas
reponame_str CONICET Digital (CONICET)
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instname_str Consejo Nacional de Investigaciones Científicas y Técnicas
repository.name.fl_str_mv CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicas
repository.mail.fl_str_mv dasensio@conicet.gov.ar; lcarlino@conicet.gov.ar
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score 13.070432

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