The secreted esterase of propionibacterium freudenreichii has a major role in cheese lipolysis
- Autores
- Abeijon Mukdsi, Maria Claudia; Falentin, Hélène; Maillard, Marie Bernadette; Chuat, Victoria; Medina, Roxana Beatriz; Parayre, Sandrine; Thierry, Anne
- Año de publicación
- 2014
- Idioma
- inglés
- Tipo de recurso
- artículo
- Estado
- versión publicada
- Descripción
- Free fatty acids are important flavor compounds in cheese. Propionibacterium freudenreichii is the main agent of their release through lipolysis in Swiss cheese. Our aim was to identify the esterase(s) involved in lipolysis by P. freudenreichii. We targeted two previously identified esterases: one secreted esterase, PF#279, and one putative cell wall-anchored esterase, PF#774. To evaluate their role in lipolysis, we constructed overexpression and knockout mutants of P. freudenreichii CIRM-BIA1T for each corresponding gene. The sequences of both genes were also compared in 21 wild-type strains. All strains were assessed for their lipolytic activity on milk fat. The lipolytic activity observed matched data previously reported in cheese, thus validating the relevance of the method used. The mutants overexpressing PF#279 or PF#774 released four times more fatty acids than the wild-type strain, demonstrating that both enzymes are lipolytic esterases. However, inactivation of the pf279 gene induced a 75% reduction in the lipolytic activity compared to that of the wild-type strain, whereas inactivation of the pf774 gene did not modify the phenotype. Two of the 21 wild-type strains tested did not display any detectable lipolytic activity. Interestingly, these two strains exhibited the same single-nucleotide deletion at the beginning of the pf279 gene sequence, leading to a premature stop codon, whereas they harbored a pf774 gene highly similar to that of the other strains. Taken together, these results clearly demonstrate that PF#279 is the main lipolytic esterase in P. freudenreichii and a key agent of Swiss cheese lipolysis.
Fil: Abeijon Mukdsi, Maria Claudia. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Tucumán. Centro de Referencia para Lactobacilos (i); Argentina. Universidad Nacional de Tucumán. Facultad de Bioquímica, Química y Farmacia; Argentina
Fil: Falentin, Hélène. INRA. Science et Technologie du Lait et de l’OEuf; Francia. Agrocampus Ouest. Science et Technologie du Lait et de l’OEuf; Francia
Fil: Maillard, Marie Bernadette. INRA. Science et Technologie du Lait et de l’OEuf; Francia. Agrocampus Ouest. Science et Technologie du Lait et de l’OEuf; Francia
Fil: Chuat, Victoria. INRA. Science et Technologie du Lait et de l’OEuf; Francia. Agrocampus Ouest. Science et Technologie du Lait et de l’OEuf; Francia
Fil: Medina, Roxana Beatriz. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Tucumán. Centro de Referencia para Lactobacilos (i); Argentina
Fil: Parayre, Sandrine. INRA. Science et Technologie du Lait et de l’OEuf; Francia. Agrocampus Ouest. Science et Technologie du Lait et de l’OEuf; Francia
Fil: Thierry, Anne. INRA. Science et Technologie du Lait et de l’OEuf; Francia. Agrocampus Ouest. Science et Technologie du Lait et de l’OEuf; Francia - Materia
-
Esterase
Propionibacterium Freudenreichii
Cheese Lipolysis - Nivel de accesibilidad
- acceso abierto
- Condiciones de uso
- https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
- Repositorio
- Institución
- Consejo Nacional de Investigaciones Científicas y Técnicas
- OAI Identificador
- oai:ri.conicet.gov.ar:11336/2908
Ver los metadatos del registro completo
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The secreted esterase of propionibacterium freudenreichii has a major role in cheese lipolysisAbeijon Mukdsi, Maria ClaudiaFalentin, HélèneMaillard, Marie BernadetteChuat, VictoriaMedina, Roxana BeatrizParayre, SandrineThierry, AnneEsterasePropionibacterium FreudenreichiiCheese Lipolysishttps://purl.org/becyt/ford/1.6https://purl.org/becyt/ford/1Free fatty acids are important flavor compounds in cheese. Propionibacterium freudenreichii is the main agent of their release through lipolysis in Swiss cheese. Our aim was to identify the esterase(s) involved in lipolysis by P. freudenreichii. We targeted two previously identified esterases: one secreted esterase, PF#279, and one putative cell wall-anchored esterase, PF#774. To evaluate their role in lipolysis, we constructed overexpression and knockout mutants of P. freudenreichii CIRM-BIA1T for each corresponding gene. The sequences of both genes were also compared in 21 wild-type strains. All strains were assessed for their lipolytic activity on milk fat. The lipolytic activity observed matched data previously reported in cheese, thus validating the relevance of the method used. The mutants overexpressing PF#279 or PF#774 released four times more fatty acids than the wild-type strain, demonstrating that both enzymes are lipolytic esterases. However, inactivation of the pf279 gene induced a 75% reduction in the lipolytic activity compared to that of the wild-type strain, whereas inactivation of the pf774 gene did not modify the phenotype. Two of the 21 wild-type strains tested did not display any detectable lipolytic activity. Interestingly, these two strains exhibited the same single-nucleotide deletion at the beginning of the pf279 gene sequence, leading to a premature stop codon, whereas they harbored a pf774 gene highly similar to that of the other strains. Taken together, these results clearly demonstrate that PF#279 is the main lipolytic esterase in P. freudenreichii and a key agent of Swiss cheese lipolysis.Fil: Abeijon Mukdsi, Maria Claudia. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Tucumán. Centro de Referencia para Lactobacilos (i); Argentina. Universidad Nacional de Tucumán. Facultad de Bioquímica, Química y Farmacia; ArgentinaFil: Falentin, Hélène. INRA. Science et Technologie du Lait et de l’OEuf; Francia. Agrocampus Ouest. Science et Technologie du Lait et de l’OEuf; FranciaFil: Maillard, Marie Bernadette. INRA. Science et Technologie du Lait et de l’OEuf; Francia. Agrocampus Ouest. Science et Technologie du Lait et de l’OEuf; FranciaFil: Chuat, Victoria. INRA. Science et Technologie du Lait et de l’OEuf; Francia. Agrocampus Ouest. Science et Technologie du Lait et de l’OEuf; FranciaFil: Medina, Roxana Beatriz. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Tucumán. Centro de Referencia para Lactobacilos (i); ArgentinaFil: Parayre, Sandrine. INRA. Science et Technologie du Lait et de l’OEuf; Francia. Agrocampus Ouest. Science et Technologie du Lait et de l’OEuf; FranciaFil: Thierry, Anne. INRA. Science et Technologie du Lait et de l’OEuf; Francia. Agrocampus Ouest. Science et Technologie du Lait et de l’OEuf; FranciaAmerican Society for Microbiology2014-01info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionhttp://purl.org/coar/resource_type/c_6501info:ar-repo/semantics/articuloapplication/pdfapplication/pdfhttp://hdl.handle.net/11336/2908Abeijon Mukdsi, Maria Claudia; Falentin, Hélène ; Maillard, Marie Bernadette ; Chuat, Victoria; Medina, Roxana Beatriz; et al.; The secreted esterase of propionibacterium freudenreichii has a major role in cheese lipolysis; American Society for Microbiology; Applied And Environmental Microbiology; 80; 2; 1-2014; 751-7560099-22401098-5336enginfo:eu-repo/semantics/altIdentifier/pmid/http://www.pubmedcentral.nih.gov/tocrender.fcgi?journal=83info:eu-repo/semantics/altIdentifier/doi/doi:10.1128/AEM.03640-13info:eu-repo/semantics/openAccesshttps://creativecommons.org/licenses/by-nc-sa/2.5/ar/reponame:CONICET Digital (CONICET)instname:Consejo Nacional de Investigaciones Científicas y Técnicas2025-10-15T15:12:32Zoai:ri.conicet.gov.ar:11336/2908instacron:CONICETInstitucionalhttp://ri.conicet.gov.ar/Organismo científico-tecnológicoNo correspondehttp://ri.conicet.gov.ar/oai/requestdasensio@conicet.gov.ar; lcarlino@conicet.gov.arArgentinaNo correspondeNo correspondeNo correspondeopendoar:34982025-10-15 15:12:32.574CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicasfalse |
dc.title.none.fl_str_mv |
The secreted esterase of propionibacterium freudenreichii has a major role in cheese lipolysis |
title |
The secreted esterase of propionibacterium freudenreichii has a major role in cheese lipolysis |
spellingShingle |
The secreted esterase of propionibacterium freudenreichii has a major role in cheese lipolysis Abeijon Mukdsi, Maria Claudia Esterase Propionibacterium Freudenreichii Cheese Lipolysis |
title_short |
The secreted esterase of propionibacterium freudenreichii has a major role in cheese lipolysis |
title_full |
The secreted esterase of propionibacterium freudenreichii has a major role in cheese lipolysis |
title_fullStr |
The secreted esterase of propionibacterium freudenreichii has a major role in cheese lipolysis |
title_full_unstemmed |
The secreted esterase of propionibacterium freudenreichii has a major role in cheese lipolysis |
title_sort |
The secreted esterase of propionibacterium freudenreichii has a major role in cheese lipolysis |
dc.creator.none.fl_str_mv |
Abeijon Mukdsi, Maria Claudia Falentin, Hélène Maillard, Marie Bernadette Chuat, Victoria Medina, Roxana Beatriz Parayre, Sandrine Thierry, Anne |
author |
Abeijon Mukdsi, Maria Claudia |
author_facet |
Abeijon Mukdsi, Maria Claudia Falentin, Hélène Maillard, Marie Bernadette Chuat, Victoria Medina, Roxana Beatriz Parayre, Sandrine Thierry, Anne |
author_role |
author |
author2 |
Falentin, Hélène Maillard, Marie Bernadette Chuat, Victoria Medina, Roxana Beatriz Parayre, Sandrine Thierry, Anne |
author2_role |
author author author author author author |
dc.subject.none.fl_str_mv |
Esterase Propionibacterium Freudenreichii Cheese Lipolysis |
topic |
Esterase Propionibacterium Freudenreichii Cheese Lipolysis |
purl_subject.fl_str_mv |
https://purl.org/becyt/ford/1.6 https://purl.org/becyt/ford/1 |
dc.description.none.fl_txt_mv |
Free fatty acids are important flavor compounds in cheese. Propionibacterium freudenreichii is the main agent of their release through lipolysis in Swiss cheese. Our aim was to identify the esterase(s) involved in lipolysis by P. freudenreichii. We targeted two previously identified esterases: one secreted esterase, PF#279, and one putative cell wall-anchored esterase, PF#774. To evaluate their role in lipolysis, we constructed overexpression and knockout mutants of P. freudenreichii CIRM-BIA1T for each corresponding gene. The sequences of both genes were also compared in 21 wild-type strains. All strains were assessed for their lipolytic activity on milk fat. The lipolytic activity observed matched data previously reported in cheese, thus validating the relevance of the method used. The mutants overexpressing PF#279 or PF#774 released four times more fatty acids than the wild-type strain, demonstrating that both enzymes are lipolytic esterases. However, inactivation of the pf279 gene induced a 75% reduction in the lipolytic activity compared to that of the wild-type strain, whereas inactivation of the pf774 gene did not modify the phenotype. Two of the 21 wild-type strains tested did not display any detectable lipolytic activity. Interestingly, these two strains exhibited the same single-nucleotide deletion at the beginning of the pf279 gene sequence, leading to a premature stop codon, whereas they harbored a pf774 gene highly similar to that of the other strains. Taken together, these results clearly demonstrate that PF#279 is the main lipolytic esterase in P. freudenreichii and a key agent of Swiss cheese lipolysis. Fil: Abeijon Mukdsi, Maria Claudia. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Tucumán. Centro de Referencia para Lactobacilos (i); Argentina. Universidad Nacional de Tucumán. Facultad de Bioquímica, Química y Farmacia; Argentina Fil: Falentin, Hélène. INRA. Science et Technologie du Lait et de l’OEuf; Francia. Agrocampus Ouest. Science et Technologie du Lait et de l’OEuf; Francia Fil: Maillard, Marie Bernadette. INRA. Science et Technologie du Lait et de l’OEuf; Francia. Agrocampus Ouest. Science et Technologie du Lait et de l’OEuf; Francia Fil: Chuat, Victoria. INRA. Science et Technologie du Lait et de l’OEuf; Francia. Agrocampus Ouest. Science et Technologie du Lait et de l’OEuf; Francia Fil: Medina, Roxana Beatriz. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Tucumán. Centro de Referencia para Lactobacilos (i); Argentina Fil: Parayre, Sandrine. INRA. Science et Technologie du Lait et de l’OEuf; Francia. Agrocampus Ouest. Science et Technologie du Lait et de l’OEuf; Francia Fil: Thierry, Anne. INRA. Science et Technologie du Lait et de l’OEuf; Francia. Agrocampus Ouest. Science et Technologie du Lait et de l’OEuf; Francia |
description |
Free fatty acids are important flavor compounds in cheese. Propionibacterium freudenreichii is the main agent of their release through lipolysis in Swiss cheese. Our aim was to identify the esterase(s) involved in lipolysis by P. freudenreichii. We targeted two previously identified esterases: one secreted esterase, PF#279, and one putative cell wall-anchored esterase, PF#774. To evaluate their role in lipolysis, we constructed overexpression and knockout mutants of P. freudenreichii CIRM-BIA1T for each corresponding gene. The sequences of both genes were also compared in 21 wild-type strains. All strains were assessed for their lipolytic activity on milk fat. The lipolytic activity observed matched data previously reported in cheese, thus validating the relevance of the method used. The mutants overexpressing PF#279 or PF#774 released four times more fatty acids than the wild-type strain, demonstrating that both enzymes are lipolytic esterases. However, inactivation of the pf279 gene induced a 75% reduction in the lipolytic activity compared to that of the wild-type strain, whereas inactivation of the pf774 gene did not modify the phenotype. Two of the 21 wild-type strains tested did not display any detectable lipolytic activity. Interestingly, these two strains exhibited the same single-nucleotide deletion at the beginning of the pf279 gene sequence, leading to a premature stop codon, whereas they harbored a pf774 gene highly similar to that of the other strains. Taken together, these results clearly demonstrate that PF#279 is the main lipolytic esterase in P. freudenreichii and a key agent of Swiss cheese lipolysis. |
publishDate |
2014 |
dc.date.none.fl_str_mv |
2014-01 |
dc.type.none.fl_str_mv |
info:eu-repo/semantics/article info:eu-repo/semantics/publishedVersion http://purl.org/coar/resource_type/c_6501 info:ar-repo/semantics/articulo |
format |
article |
status_str |
publishedVersion |
dc.identifier.none.fl_str_mv |
http://hdl.handle.net/11336/2908 Abeijon Mukdsi, Maria Claudia; Falentin, Hélène ; Maillard, Marie Bernadette ; Chuat, Victoria; Medina, Roxana Beatriz; et al.; The secreted esterase of propionibacterium freudenreichii has a major role in cheese lipolysis; American Society for Microbiology; Applied And Environmental Microbiology; 80; 2; 1-2014; 751-756 0099-2240 1098-5336 |
url |
http://hdl.handle.net/11336/2908 |
identifier_str_mv |
Abeijon Mukdsi, Maria Claudia; Falentin, Hélène ; Maillard, Marie Bernadette ; Chuat, Victoria; Medina, Roxana Beatriz; et al.; The secreted esterase of propionibacterium freudenreichii has a major role in cheese lipolysis; American Society for Microbiology; Applied And Environmental Microbiology; 80; 2; 1-2014; 751-756 0099-2240 1098-5336 |
dc.language.none.fl_str_mv |
eng |
language |
eng |
dc.relation.none.fl_str_mv |
info:eu-repo/semantics/altIdentifier/pmid/http://www.pubmedcentral.nih.gov/tocrender.fcgi?journal=83 info:eu-repo/semantics/altIdentifier/doi/doi:10.1128/AEM.03640-13 |
dc.rights.none.fl_str_mv |
info:eu-repo/semantics/openAccess https://creativecommons.org/licenses/by-nc-sa/2.5/ar/ |
eu_rights_str_mv |
openAccess |
rights_invalid_str_mv |
https://creativecommons.org/licenses/by-nc-sa/2.5/ar/ |
dc.format.none.fl_str_mv |
application/pdf application/pdf |
dc.publisher.none.fl_str_mv |
American Society for Microbiology |
publisher.none.fl_str_mv |
American Society for Microbiology |
dc.source.none.fl_str_mv |
reponame:CONICET Digital (CONICET) instname:Consejo Nacional de Investigaciones Científicas y Técnicas |
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CONICET Digital (CONICET) |
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Consejo Nacional de Investigaciones Científicas y Técnicas |
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CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicas |
repository.mail.fl_str_mv |
dasensio@conicet.gov.ar; lcarlino@conicet.gov.ar |
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1846083273735798784 |
score |
13.22299 |