Phenotypic detection of plasmid-mediated colistin resistance in Enterobacteriaceae

Autores
Gonzales Escalante, Edgar; Yauri Condor, Katherine; Di Conza, José Alejandro; Gutkind, Gabriel Osvaldo
Año de publicación
2019
Idioma
inglés
Tipo de recurso
artículo
Estado
versión publicada
Descripción
The aim of this work was to evaluate an easy-to-perform assay basedupon inhibition of mobile colistin resistance (MCR) activity by EDTA. We included 92nonrelated isolates of Enterobacteriaceae (74 Escherichia coli, 17 Klebsiella pneumoniae,and 1 Serratia marcescens). Our proposed method is based on a modificationof the colistin agar-spot screening test (CAST), a plate containing 3 g/ml colistin,by adding an extra plate of colistin agar-spot supplemented with EDTA (eCAST).Bacterial growth was evaluated after 24 h of incubation at 35°C. All the colistinresistantisolates showed development on the CAST plates. Colistin-resistant K. pneumoniaewithout mcr-1 and S. marcescens also grew on the eCAST plates. In contrast,colistin-resistant MCR-producing E. coli was not able to grow in eCAST plates. Thecombined CAST/eCAST test could provide a simple and easy-to-perform method todifferentiate MCR-producing Enterobacteriaceae from those in which colistin resistanceis mediated by chromosomal mechanisms.
Fil: Gonzales Escalante, Edgar. Universidad de Buenos Aires. Facultad de Farmacia y Bioquímica. Departamento de Microbiología, Inmunología y Biotecnología. Cátedra de Microbiología; Argentina
Fil: Yauri Condor, Katherine. Universidad Nacional Mayor de San Marcos; Perú
Fil: Di Conza, José Alejandro. Universidad de Buenos Aires. Facultad de Farmacia y Bioquímica. Departamento de Microbiología, Inmunología y Biotecnología. Cátedra de Microbiología; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Houssay; Argentina
Fil: Gutkind, Gabriel Osvaldo. Universidad de Buenos Aires. Facultad de Farmacia y Bioquímica. Departamento de Microbiología, Inmunología y Biotecnología. Cátedra de Microbiología; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Houssay; Argentina
Materia
MCR
ENTEROBACTERIACEAE
COLISTIN
EDTA
Nivel de accesibilidad
acceso abierto
Condiciones de uso
https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
Repositorio
CONICET Digital (CONICET)
Institución
Consejo Nacional de Investigaciones Científicas y Técnicas
OAI Identificador
oai:ri.conicet.gov.ar:11336/109116

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spelling Phenotypic detection of plasmid-mediated colistin resistance in EnterobacteriaceaeGonzales Escalante, EdgarYauri Condor, KatherineDi Conza, José AlejandroGutkind, Gabriel OsvaldoMCRENTEROBACTERIACEAECOLISTINEDTAhttps://purl.org/becyt/ford/3.3https://purl.org/becyt/ford/3The aim of this work was to evaluate an easy-to-perform assay basedupon inhibition of mobile colistin resistance (MCR) activity by EDTA. We included 92nonrelated isolates of Enterobacteriaceae (74 Escherichia coli, 17 Klebsiella pneumoniae,and 1 Serratia marcescens). Our proposed method is based on a modificationof the colistin agar-spot screening test (CAST), a plate containing 3 g/ml colistin,by adding an extra plate of colistin agar-spot supplemented with EDTA (eCAST).Bacterial growth was evaluated after 24 h of incubation at 35°C. All the colistinresistantisolates showed development on the CAST plates. Colistin-resistant K. pneumoniaewithout mcr-1 and S. marcescens also grew on the eCAST plates. In contrast,colistin-resistant MCR-producing E. coli was not able to grow in eCAST plates. Thecombined CAST/eCAST test could provide a simple and easy-to-perform method todifferentiate MCR-producing Enterobacteriaceae from those in which colistin resistanceis mediated by chromosomal mechanisms.Fil: Gonzales Escalante, Edgar. Universidad de Buenos Aires. Facultad de Farmacia y Bioquímica. Departamento de Microbiología, Inmunología y Biotecnología. Cátedra de Microbiología; ArgentinaFil: Yauri Condor, Katherine. Universidad Nacional Mayor de San Marcos; PerúFil: Di Conza, José Alejandro. Universidad de Buenos Aires. Facultad de Farmacia y Bioquímica. Departamento de Microbiología, Inmunología y Biotecnología. Cátedra de Microbiología; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Houssay; ArgentinaFil: Gutkind, Gabriel Osvaldo. Universidad de Buenos Aires. Facultad de Farmacia y Bioquímica. Departamento de Microbiología, Inmunología y Biotecnología. Cátedra de Microbiología; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Houssay; ArgentinaAmerican Society for Microbiology2019-12info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionhttp://purl.org/coar/resource_type/c_6501info:ar-repo/semantics/articuloapplication/pdfapplication/pdfhttp://hdl.handle.net/11336/109116Gonzales Escalante, Edgar; Yauri Condor, Katherine; Di Conza, José Alejandro; Gutkind, Gabriel Osvaldo; Phenotypic detection of plasmid-mediated colistin resistance in Enterobacteriaceae; American Society for Microbiology; Journal of Clinical Microbiology; 12-2019; 1-160095-1137CONICET DigitalCONICETenginfo:eu-repo/semantics/altIdentifier/url/http://jcm.asm.org/lookup/doi/10.1128/JCM.01555-19info:eu-repo/semantics/altIdentifier/doi/10.1128/JCM.01555-19info:eu-repo/semantics/openAccesshttps://creativecommons.org/licenses/by-nc-sa/2.5/ar/reponame:CONICET Digital (CONICET)instname:Consejo Nacional de Investigaciones Científicas y Técnicas2025-09-29T09:57:57Zoai:ri.conicet.gov.ar:11336/109116instacron:CONICETInstitucionalhttp://ri.conicet.gov.ar/Organismo científico-tecnológicoNo correspondehttp://ri.conicet.gov.ar/oai/requestdasensio@conicet.gov.ar; lcarlino@conicet.gov.arArgentinaNo correspondeNo correspondeNo correspondeopendoar:34982025-09-29 09:57:57.306CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicasfalse
dc.title.none.fl_str_mv Phenotypic detection of plasmid-mediated colistin resistance in Enterobacteriaceae
title Phenotypic detection of plasmid-mediated colistin resistance in Enterobacteriaceae
spellingShingle Phenotypic detection of plasmid-mediated colistin resistance in Enterobacteriaceae
Gonzales Escalante, Edgar
MCR
ENTEROBACTERIACEAE
COLISTIN
EDTA
title_short Phenotypic detection of plasmid-mediated colistin resistance in Enterobacteriaceae
title_full Phenotypic detection of plasmid-mediated colistin resistance in Enterobacteriaceae
title_fullStr Phenotypic detection of plasmid-mediated colistin resistance in Enterobacteriaceae
title_full_unstemmed Phenotypic detection of plasmid-mediated colistin resistance in Enterobacteriaceae
title_sort Phenotypic detection of plasmid-mediated colistin resistance in Enterobacteriaceae
dc.creator.none.fl_str_mv Gonzales Escalante, Edgar
Yauri Condor, Katherine
Di Conza, José Alejandro
Gutkind, Gabriel Osvaldo
author Gonzales Escalante, Edgar
author_facet Gonzales Escalante, Edgar
Yauri Condor, Katherine
Di Conza, José Alejandro
Gutkind, Gabriel Osvaldo
author_role author
author2 Yauri Condor, Katherine
Di Conza, José Alejandro
Gutkind, Gabriel Osvaldo
author2_role author
author
author
dc.subject.none.fl_str_mv MCR
ENTEROBACTERIACEAE
COLISTIN
EDTA
topic MCR
ENTEROBACTERIACEAE
COLISTIN
EDTA
purl_subject.fl_str_mv https://purl.org/becyt/ford/3.3
https://purl.org/becyt/ford/3
dc.description.none.fl_txt_mv The aim of this work was to evaluate an easy-to-perform assay basedupon inhibition of mobile colistin resistance (MCR) activity by EDTA. We included 92nonrelated isolates of Enterobacteriaceae (74 Escherichia coli, 17 Klebsiella pneumoniae,and 1 Serratia marcescens). Our proposed method is based on a modificationof the colistin agar-spot screening test (CAST), a plate containing 3 g/ml colistin,by adding an extra plate of colistin agar-spot supplemented with EDTA (eCAST).Bacterial growth was evaluated after 24 h of incubation at 35°C. All the colistinresistantisolates showed development on the CAST plates. Colistin-resistant K. pneumoniaewithout mcr-1 and S. marcescens also grew on the eCAST plates. In contrast,colistin-resistant MCR-producing E. coli was not able to grow in eCAST plates. Thecombined CAST/eCAST test could provide a simple and easy-to-perform method todifferentiate MCR-producing Enterobacteriaceae from those in which colistin resistanceis mediated by chromosomal mechanisms.
Fil: Gonzales Escalante, Edgar. Universidad de Buenos Aires. Facultad de Farmacia y Bioquímica. Departamento de Microbiología, Inmunología y Biotecnología. Cátedra de Microbiología; Argentina
Fil: Yauri Condor, Katherine. Universidad Nacional Mayor de San Marcos; Perú
Fil: Di Conza, José Alejandro. Universidad de Buenos Aires. Facultad de Farmacia y Bioquímica. Departamento de Microbiología, Inmunología y Biotecnología. Cátedra de Microbiología; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Houssay; Argentina
Fil: Gutkind, Gabriel Osvaldo. Universidad de Buenos Aires. Facultad de Farmacia y Bioquímica. Departamento de Microbiología, Inmunología y Biotecnología. Cátedra de Microbiología; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Houssay; Argentina
description The aim of this work was to evaluate an easy-to-perform assay basedupon inhibition of mobile colistin resistance (MCR) activity by EDTA. We included 92nonrelated isolates of Enterobacteriaceae (74 Escherichia coli, 17 Klebsiella pneumoniae,and 1 Serratia marcescens). Our proposed method is based on a modificationof the colistin agar-spot screening test (CAST), a plate containing 3 g/ml colistin,by adding an extra plate of colistin agar-spot supplemented with EDTA (eCAST).Bacterial growth was evaluated after 24 h of incubation at 35°C. All the colistinresistantisolates showed development on the CAST plates. Colistin-resistant K. pneumoniaewithout mcr-1 and S. marcescens also grew on the eCAST plates. In contrast,colistin-resistant MCR-producing E. coli was not able to grow in eCAST plates. Thecombined CAST/eCAST test could provide a simple and easy-to-perform method todifferentiate MCR-producing Enterobacteriaceae from those in which colistin resistanceis mediated by chromosomal mechanisms.
publishDate 2019
dc.date.none.fl_str_mv 2019-12
dc.type.none.fl_str_mv info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
http://purl.org/coar/resource_type/c_6501
info:ar-repo/semantics/articulo
format article
status_str publishedVersion
dc.identifier.none.fl_str_mv http://hdl.handle.net/11336/109116
Gonzales Escalante, Edgar; Yauri Condor, Katherine; Di Conza, José Alejandro; Gutkind, Gabriel Osvaldo; Phenotypic detection of plasmid-mediated colistin resistance in Enterobacteriaceae; American Society for Microbiology; Journal of Clinical Microbiology; 12-2019; 1-16
0095-1137
CONICET Digital
CONICET
url http://hdl.handle.net/11336/109116
identifier_str_mv Gonzales Escalante, Edgar; Yauri Condor, Katherine; Di Conza, José Alejandro; Gutkind, Gabriel Osvaldo; Phenotypic detection of plasmid-mediated colistin resistance in Enterobacteriaceae; American Society for Microbiology; Journal of Clinical Microbiology; 12-2019; 1-16
0095-1137
CONICET Digital
CONICET
dc.language.none.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv info:eu-repo/semantics/altIdentifier/url/http://jcm.asm.org/lookup/doi/10.1128/JCM.01555-19
info:eu-repo/semantics/altIdentifier/doi/10.1128/JCM.01555-19
dc.rights.none.fl_str_mv info:eu-repo/semantics/openAccess
https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
eu_rights_str_mv openAccess
rights_invalid_str_mv https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
dc.format.none.fl_str_mv application/pdf
application/pdf
dc.publisher.none.fl_str_mv American Society for Microbiology
publisher.none.fl_str_mv American Society for Microbiology
dc.source.none.fl_str_mv reponame:CONICET Digital (CONICET)
instname:Consejo Nacional de Investigaciones Científicas y Técnicas
reponame_str CONICET Digital (CONICET)
collection CONICET Digital (CONICET)
instname_str Consejo Nacional de Investigaciones Científicas y Técnicas
repository.name.fl_str_mv CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicas
repository.mail.fl_str_mv dasensio@conicet.gov.ar; lcarlino@conicet.gov.ar
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