Phenotypic detection of plasmid-mediated colistin resistance in Enterobacteriaceae
- Autores
- Gonzales Escalante, Edgar; Yauri Condor, Katherine; Di Conza, José Alejandro; Gutkind, Gabriel Osvaldo
- Año de publicación
- 2019
- Idioma
- inglés
- Tipo de recurso
- artículo
- Estado
- versión publicada
- Descripción
- The aim of this work was to evaluate an easy-to-perform assay basedupon inhibition of mobile colistin resistance (MCR) activity by EDTA. We included 92nonrelated isolates of Enterobacteriaceae (74 Escherichia coli, 17 Klebsiella pneumoniae,and 1 Serratia marcescens). Our proposed method is based on a modificationof the colistin agar-spot screening test (CAST), a plate containing 3 g/ml colistin,by adding an extra plate of colistin agar-spot supplemented with EDTA (eCAST).Bacterial growth was evaluated after 24 h of incubation at 35°C. All the colistinresistantisolates showed development on the CAST plates. Colistin-resistant K. pneumoniaewithout mcr-1 and S. marcescens also grew on the eCAST plates. In contrast,colistin-resistant MCR-producing E. coli was not able to grow in eCAST plates. Thecombined CAST/eCAST test could provide a simple and easy-to-perform method todifferentiate MCR-producing Enterobacteriaceae from those in which colistin resistanceis mediated by chromosomal mechanisms.
Fil: Gonzales Escalante, Edgar. Universidad de Buenos Aires. Facultad de Farmacia y Bioquímica. Departamento de Microbiología, Inmunología y Biotecnología. Cátedra de Microbiología; Argentina
Fil: Yauri Condor, Katherine. Universidad Nacional Mayor de San Marcos; Perú
Fil: Di Conza, José Alejandro. Universidad de Buenos Aires. Facultad de Farmacia y Bioquímica. Departamento de Microbiología, Inmunología y Biotecnología. Cátedra de Microbiología; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Houssay; Argentina
Fil: Gutkind, Gabriel Osvaldo. Universidad de Buenos Aires. Facultad de Farmacia y Bioquímica. Departamento de Microbiología, Inmunología y Biotecnología. Cátedra de Microbiología; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Houssay; Argentina - Materia
-
MCR
ENTEROBACTERIACEAE
COLISTIN
EDTA - Nivel de accesibilidad
- acceso abierto
- Condiciones de uso
- https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
- Repositorio
.jpg)
- Institución
- Consejo Nacional de Investigaciones Científicas y Técnicas
- OAI Identificador
- oai:ri.conicet.gov.ar:11336/109116
Ver los metadatos del registro completo
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Phenotypic detection of plasmid-mediated colistin resistance in EnterobacteriaceaeGonzales Escalante, EdgarYauri Condor, KatherineDi Conza, José AlejandroGutkind, Gabriel OsvaldoMCRENTEROBACTERIACEAECOLISTINEDTAhttps://purl.org/becyt/ford/3.3https://purl.org/becyt/ford/3The aim of this work was to evaluate an easy-to-perform assay basedupon inhibition of mobile colistin resistance (MCR) activity by EDTA. We included 92nonrelated isolates of Enterobacteriaceae (74 Escherichia coli, 17 Klebsiella pneumoniae,and 1 Serratia marcescens). Our proposed method is based on a modificationof the colistin agar-spot screening test (CAST), a plate containing 3 g/ml colistin,by adding an extra plate of colistin agar-spot supplemented with EDTA (eCAST).Bacterial growth was evaluated after 24 h of incubation at 35°C. All the colistinresistantisolates showed development on the CAST plates. Colistin-resistant K. pneumoniaewithout mcr-1 and S. marcescens also grew on the eCAST plates. In contrast,colistin-resistant MCR-producing E. coli was not able to grow in eCAST plates. Thecombined CAST/eCAST test could provide a simple and easy-to-perform method todifferentiate MCR-producing Enterobacteriaceae from those in which colistin resistanceis mediated by chromosomal mechanisms.Fil: Gonzales Escalante, Edgar. Universidad de Buenos Aires. Facultad de Farmacia y Bioquímica. Departamento de Microbiología, Inmunología y Biotecnología. Cátedra de Microbiología; ArgentinaFil: Yauri Condor, Katherine. Universidad Nacional Mayor de San Marcos; PerúFil: Di Conza, José Alejandro. Universidad de Buenos Aires. Facultad de Farmacia y Bioquímica. Departamento de Microbiología, Inmunología y Biotecnología. Cátedra de Microbiología; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Houssay; ArgentinaFil: Gutkind, Gabriel Osvaldo. Universidad de Buenos Aires. Facultad de Farmacia y Bioquímica. Departamento de Microbiología, Inmunología y Biotecnología. Cátedra de Microbiología; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Houssay; ArgentinaAmerican Society for Microbiology2019-12info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionhttp://purl.org/coar/resource_type/c_6501info:ar-repo/semantics/articuloapplication/pdfapplication/pdfhttp://hdl.handle.net/11336/109116Gonzales Escalante, Edgar; Yauri Condor, Katherine; Di Conza, José Alejandro; Gutkind, Gabriel Osvaldo; Phenotypic detection of plasmid-mediated colistin resistance in Enterobacteriaceae; American Society for Microbiology; Journal of Clinical Microbiology; 12-2019; 1-160095-1137CONICET DigitalCONICETenginfo:eu-repo/semantics/altIdentifier/url/http://jcm.asm.org/lookup/doi/10.1128/JCM.01555-19info:eu-repo/semantics/altIdentifier/doi/10.1128/JCM.01555-19info:eu-repo/semantics/openAccesshttps://creativecommons.org/licenses/by-nc-sa/2.5/ar/reponame:CONICET Digital (CONICET)instname:Consejo Nacional de Investigaciones Científicas y Técnicas2025-10-22T11:27:14Zoai:ri.conicet.gov.ar:11336/109116instacron:CONICETInstitucionalhttp://ri.conicet.gov.ar/Organismo científico-tecnológicoNo correspondehttp://ri.conicet.gov.ar/oai/requestdasensio@conicet.gov.ar; lcarlino@conicet.gov.arArgentinaNo correspondeNo correspondeNo correspondeopendoar:34982025-10-22 11:27:14.617CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicasfalse |
| dc.title.none.fl_str_mv |
Phenotypic detection of plasmid-mediated colistin resistance in Enterobacteriaceae |
| title |
Phenotypic detection of plasmid-mediated colistin resistance in Enterobacteriaceae |
| spellingShingle |
Phenotypic detection of plasmid-mediated colistin resistance in Enterobacteriaceae Gonzales Escalante, Edgar MCR ENTEROBACTERIACEAE COLISTIN EDTA |
| title_short |
Phenotypic detection of plasmid-mediated colistin resistance in Enterobacteriaceae |
| title_full |
Phenotypic detection of plasmid-mediated colistin resistance in Enterobacteriaceae |
| title_fullStr |
Phenotypic detection of plasmid-mediated colistin resistance in Enterobacteriaceae |
| title_full_unstemmed |
Phenotypic detection of plasmid-mediated colistin resistance in Enterobacteriaceae |
| title_sort |
Phenotypic detection of plasmid-mediated colistin resistance in Enterobacteriaceae |
| dc.creator.none.fl_str_mv |
Gonzales Escalante, Edgar Yauri Condor, Katherine Di Conza, José Alejandro Gutkind, Gabriel Osvaldo |
| author |
Gonzales Escalante, Edgar |
| author_facet |
Gonzales Escalante, Edgar Yauri Condor, Katherine Di Conza, José Alejandro Gutkind, Gabriel Osvaldo |
| author_role |
author |
| author2 |
Yauri Condor, Katherine Di Conza, José Alejandro Gutkind, Gabriel Osvaldo |
| author2_role |
author author author |
| dc.subject.none.fl_str_mv |
MCR ENTEROBACTERIACEAE COLISTIN EDTA |
| topic |
MCR ENTEROBACTERIACEAE COLISTIN EDTA |
| purl_subject.fl_str_mv |
https://purl.org/becyt/ford/3.3 https://purl.org/becyt/ford/3 |
| dc.description.none.fl_txt_mv |
The aim of this work was to evaluate an easy-to-perform assay basedupon inhibition of mobile colistin resistance (MCR) activity by EDTA. We included 92nonrelated isolates of Enterobacteriaceae (74 Escherichia coli, 17 Klebsiella pneumoniae,and 1 Serratia marcescens). Our proposed method is based on a modificationof the colistin agar-spot screening test (CAST), a plate containing 3 g/ml colistin,by adding an extra plate of colistin agar-spot supplemented with EDTA (eCAST).Bacterial growth was evaluated after 24 h of incubation at 35°C. All the colistinresistantisolates showed development on the CAST plates. Colistin-resistant K. pneumoniaewithout mcr-1 and S. marcescens also grew on the eCAST plates. In contrast,colistin-resistant MCR-producing E. coli was not able to grow in eCAST plates. Thecombined CAST/eCAST test could provide a simple and easy-to-perform method todifferentiate MCR-producing Enterobacteriaceae from those in which colistin resistanceis mediated by chromosomal mechanisms. Fil: Gonzales Escalante, Edgar. Universidad de Buenos Aires. Facultad de Farmacia y Bioquímica. Departamento de Microbiología, Inmunología y Biotecnología. Cátedra de Microbiología; Argentina Fil: Yauri Condor, Katherine. Universidad Nacional Mayor de San Marcos; Perú Fil: Di Conza, José Alejandro. Universidad de Buenos Aires. Facultad de Farmacia y Bioquímica. Departamento de Microbiología, Inmunología y Biotecnología. Cátedra de Microbiología; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Houssay; Argentina Fil: Gutkind, Gabriel Osvaldo. Universidad de Buenos Aires. Facultad de Farmacia y Bioquímica. Departamento de Microbiología, Inmunología y Biotecnología. Cátedra de Microbiología; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Houssay; Argentina |
| description |
The aim of this work was to evaluate an easy-to-perform assay basedupon inhibition of mobile colistin resistance (MCR) activity by EDTA. We included 92nonrelated isolates of Enterobacteriaceae (74 Escherichia coli, 17 Klebsiella pneumoniae,and 1 Serratia marcescens). Our proposed method is based on a modificationof the colistin agar-spot screening test (CAST), a plate containing 3 g/ml colistin,by adding an extra plate of colistin agar-spot supplemented with EDTA (eCAST).Bacterial growth was evaluated after 24 h of incubation at 35°C. All the colistinresistantisolates showed development on the CAST plates. Colistin-resistant K. pneumoniaewithout mcr-1 and S. marcescens also grew on the eCAST plates. In contrast,colistin-resistant MCR-producing E. coli was not able to grow in eCAST plates. Thecombined CAST/eCAST test could provide a simple and easy-to-perform method todifferentiate MCR-producing Enterobacteriaceae from those in which colistin resistanceis mediated by chromosomal mechanisms. |
| publishDate |
2019 |
| dc.date.none.fl_str_mv |
2019-12 |
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info:eu-repo/semantics/article info:eu-repo/semantics/publishedVersion http://purl.org/coar/resource_type/c_6501 info:ar-repo/semantics/articulo |
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article |
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publishedVersion |
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http://hdl.handle.net/11336/109116 Gonzales Escalante, Edgar; Yauri Condor, Katherine; Di Conza, José Alejandro; Gutkind, Gabriel Osvaldo; Phenotypic detection of plasmid-mediated colistin resistance in Enterobacteriaceae; American Society for Microbiology; Journal of Clinical Microbiology; 12-2019; 1-16 0095-1137 CONICET Digital CONICET |
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http://hdl.handle.net/11336/109116 |
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Gonzales Escalante, Edgar; Yauri Condor, Katherine; Di Conza, José Alejandro; Gutkind, Gabriel Osvaldo; Phenotypic detection of plasmid-mediated colistin resistance in Enterobacteriaceae; American Society for Microbiology; Journal of Clinical Microbiology; 12-2019; 1-16 0095-1137 CONICET Digital CONICET |
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eng |
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eng |
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American Society for Microbiology |
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