Fungal-Derived tRNAs Are Expressed and Aminoacylated in Orchid Mitochondria

Autores
Warren, Jessica M.; Ceriotti, Luis Federico; Sánchez Puerta, María Virginia; Sloan, Daniel B.
Año de publicación
2025
Idioma
inglés
Tipo de recurso
artículo
Estado
versión publicada
Descripción
Plant mitochondrial genomes (mitogenomes) experience remarkable levels of horizontal gene transfer, including the recent discovery that orchids anciently acquired DNA from fungal mitogenomes. Thus far, however, there is no evidence that any of the genes from this interkingdom horizontal gene transfer are functional in orchid mitogenomes. Here, we applied a specialized sequencing approach to the orchid Corallorhiza maculata and found that some fungal-derived tRNA genes in the transferred region are transcribed, post-transcriptionally modified, and aminoacylated. In contrast, all the transferred protein-coding sequences appear to be pseudogenes. These findings show that fungal horizontal gene transfer has altered the composition of the orchid mitochondrial tRNA pool and suggest that these foreign tRNAs function in translation. The exceptional capacity of tRNAs for horizontal gene transfer and functional replacement is further illustrated by the diversity of tRNA genes in the C. maculata mitogenome, which also include genes of plastid and bacterial origin in addition to their native mitochondrial counterparts.
Fil: Warren, Jessica M.. Arizona State University; Estados Unidos. Howard Hughes Medical Institute; Estados Unidos
Fil: Ceriotti, Luis Federico. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Mendoza. Instituto de Biología Agrícola de Mendoza. Universidad Nacional de Cuyo. Facultad de Ciencias Agrarias. Instituto de Biología Agrícola de Mendoza; Argentina. Universidad Nacional de Cuyo. Facultad de Ciencias Exactas y Naturales; Argentina
Fil: Sánchez Puerta, María Virginia. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Mendoza. Instituto de Biología Agrícola de Mendoza. Universidad Nacional de Cuyo. Facultad de Ciencias Agrarias. Instituto de Biología Agrícola de Mendoza; Argentina. Universidad Nacional de Cuyo. Facultad de Ciencias Exactas y Naturales; Argentina
Fil: Sloan, Daniel B.. State University Of Colorado-fort Collins. Dept.of Biology; Estados Unidos
Materia
HORIZONTAL GENE TRANSFER
TRNA
AMINOACYLATION
MITOCHONDRIAL GENOME
Nivel de accesibilidad
acceso abierto
Condiciones de uso
https://creativecommons.org/licenses/by/2.5/ar/
Repositorio
CONICET Digital (CONICET)
Institución
Consejo Nacional de Investigaciones Científicas y Técnicas
OAI Identificador
oai:ri.conicet.gov.ar:11336/282285
Acceder
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network_name_str CONICET Digital (CONICET)
spelling Fungal-Derived tRNAs Are Expressed and Aminoacylated in Orchid MitochondriaWarren, Jessica M.Ceriotti, Luis FedericoSánchez Puerta, María VirginiaSloan, Daniel B.HORIZONTAL GENE TRANSFERTRNAAMINOACYLATIONMITOCHONDRIAL GENOMEhttps://purl.org/becyt/ford/1.6https://purl.org/becyt/ford/1Plant mitochondrial genomes (mitogenomes) experience remarkable levels of horizontal gene transfer, including the recent discovery that orchids anciently acquired DNA from fungal mitogenomes. Thus far, however, there is no evidence that any of the genes from this interkingdom horizontal gene transfer are functional in orchid mitogenomes. Here, we applied a specialized sequencing approach to the orchid Corallorhiza maculata and found that some fungal-derived tRNA genes in the transferred region are transcribed, post-transcriptionally modified, and aminoacylated. In contrast, all the transferred protein-coding sequences appear to be pseudogenes. These findings show that fungal horizontal gene transfer has altered the composition of the orchid mitochondrial tRNA pool and suggest that these foreign tRNAs function in translation. The exceptional capacity of tRNAs for horizontal gene transfer and functional replacement is further illustrated by the diversity of tRNA genes in the C. maculata mitogenome, which also include genes of plastid and bacterial origin in addition to their native mitochondrial counterparts.Fil: Warren, Jessica M.. Arizona State University; Estados Unidos. Howard Hughes Medical Institute; Estados UnidosFil: Ceriotti, Luis Federico. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Mendoza. Instituto de Biología Agrícola de Mendoza. Universidad Nacional de Cuyo. Facultad de Ciencias Agrarias. Instituto de Biología Agrícola de Mendoza; Argentina. Universidad Nacional de Cuyo. Facultad de Ciencias Exactas y Naturales; ArgentinaFil: Sánchez Puerta, María Virginia. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Mendoza. Instituto de Biología Agrícola de Mendoza. Universidad Nacional de Cuyo. Facultad de Ciencias Agrarias. Instituto de Biología Agrícola de Mendoza; Argentina. Universidad Nacional de Cuyo. Facultad de Ciencias Exactas y Naturales; ArgentinaFil: Sloan, Daniel B.. State University Of Colorado-fort Collins. Dept.of Biology; Estados UnidosOxford University Press2025-01-30info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionhttp://purl.org/coar/resource_type/c_6501info:ar-repo/semantics/articuloapplication/pdfapplication/pdfapplication/pdfhttp://hdl.handle.net/11336/282285Warren, Jessica M.; Ceriotti, Luis Federico; Sánchez Puerta, María Virginia; Sloan, Daniel B.; Fungal-Derived tRNAs Are Expressed and Aminoacylated in Orchid Mitochondria; Oxford University Press; Molecular Biology and Evolution; 42; 2; 30-1-2025; 1-60737-4038CONICET DigitalCONICETenginfo:eu-repo/semantics/altIdentifier/url/https://academic.oup.com/mbe/article/doi/10.1093/molbev/msaf025/7990429info:eu-repo/semantics/altIdentifier/doi/10.1093/molbev/msaf025info:eu-repo/semantics/openAccesshttps://creativecommons.org/licenses/by/2.5/ar/reponame:CONICET Digital (CONICET)instname:Consejo Nacional de Investigaciones Científicas y Técnicas2026-03-11T12:30:53Zoai:ri.conicet.gov.ar:11336/282285instacron:CONICETInstitucionalhttp://ri.conicet.gov.ar/Organismo científico-tecnológicoNo correspondehttp://ri.conicet.gov.ar/oai/requestdasensio@conicet.gov.ar; lcarlino@conicet.gov.arArgentinaNo correspondeNo correspondeNo correspondeopendoar:34982026-03-11 12:30:53.924CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicasfalse
dc.title.none.fl_str_mv Fungal-Derived tRNAs Are Expressed and Aminoacylated in Orchid Mitochondria
title Fungal-Derived tRNAs Are Expressed and Aminoacylated in Orchid Mitochondria
spellingShingle Fungal-Derived tRNAs Are Expressed and Aminoacylated in Orchid Mitochondria
Warren, Jessica M.
HORIZONTAL GENE TRANSFER
TRNA
AMINOACYLATION
MITOCHONDRIAL GENOME
title_short Fungal-Derived tRNAs Are Expressed and Aminoacylated in Orchid Mitochondria
title_full Fungal-Derived tRNAs Are Expressed and Aminoacylated in Orchid Mitochondria
title_fullStr Fungal-Derived tRNAs Are Expressed and Aminoacylated in Orchid Mitochondria
title_full_unstemmed Fungal-Derived tRNAs Are Expressed and Aminoacylated in Orchid Mitochondria
title_sort Fungal-Derived tRNAs Are Expressed and Aminoacylated in Orchid Mitochondria
dc.creator.none.fl_str_mv Warren, Jessica M.
Ceriotti, Luis Federico
Sánchez Puerta, María Virginia
Sloan, Daniel B.
author Warren, Jessica M.
author_facet Warren, Jessica M.
Ceriotti, Luis Federico
Sánchez Puerta, María Virginia
Sloan, Daniel B.
author_role author
author2 Ceriotti, Luis Federico
Sánchez Puerta, María Virginia
Sloan, Daniel B.
author2_role author
author
author
dc.subject.none.fl_str_mv HORIZONTAL GENE TRANSFER
TRNA
AMINOACYLATION
MITOCHONDRIAL GENOME
topic HORIZONTAL GENE TRANSFER
TRNA
AMINOACYLATION
MITOCHONDRIAL GENOME
purl_subject.fl_str_mv https://purl.org/becyt/ford/1.6
https://purl.org/becyt/ford/1
dc.description.none.fl_txt_mv Plant mitochondrial genomes (mitogenomes) experience remarkable levels of horizontal gene transfer, including the recent discovery that orchids anciently acquired DNA from fungal mitogenomes. Thus far, however, there is no evidence that any of the genes from this interkingdom horizontal gene transfer are functional in orchid mitogenomes. Here, we applied a specialized sequencing approach to the orchid Corallorhiza maculata and found that some fungal-derived tRNA genes in the transferred region are transcribed, post-transcriptionally modified, and aminoacylated. In contrast, all the transferred protein-coding sequences appear to be pseudogenes. These findings show that fungal horizontal gene transfer has altered the composition of the orchid mitochondrial tRNA pool and suggest that these foreign tRNAs function in translation. The exceptional capacity of tRNAs for horizontal gene transfer and functional replacement is further illustrated by the diversity of tRNA genes in the C. maculata mitogenome, which also include genes of plastid and bacterial origin in addition to their native mitochondrial counterparts.
Fil: Warren, Jessica M.. Arizona State University; Estados Unidos. Howard Hughes Medical Institute; Estados Unidos
Fil: Ceriotti, Luis Federico. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Mendoza. Instituto de Biología Agrícola de Mendoza. Universidad Nacional de Cuyo. Facultad de Ciencias Agrarias. Instituto de Biología Agrícola de Mendoza; Argentina. Universidad Nacional de Cuyo. Facultad de Ciencias Exactas y Naturales; Argentina
Fil: Sánchez Puerta, María Virginia. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Mendoza. Instituto de Biología Agrícola de Mendoza. Universidad Nacional de Cuyo. Facultad de Ciencias Agrarias. Instituto de Biología Agrícola de Mendoza; Argentina. Universidad Nacional de Cuyo. Facultad de Ciencias Exactas y Naturales; Argentina
Fil: Sloan, Daniel B.. State University Of Colorado-fort Collins. Dept.of Biology; Estados Unidos
description Plant mitochondrial genomes (mitogenomes) experience remarkable levels of horizontal gene transfer, including the recent discovery that orchids anciently acquired DNA from fungal mitogenomes. Thus far, however, there is no evidence that any of the genes from this interkingdom horizontal gene transfer are functional in orchid mitogenomes. Here, we applied a specialized sequencing approach to the orchid Corallorhiza maculata and found that some fungal-derived tRNA genes in the transferred region are transcribed, post-transcriptionally modified, and aminoacylated. In contrast, all the transferred protein-coding sequences appear to be pseudogenes. These findings show that fungal horizontal gene transfer has altered the composition of the orchid mitochondrial tRNA pool and suggest that these foreign tRNAs function in translation. The exceptional capacity of tRNAs for horizontal gene transfer and functional replacement is further illustrated by the diversity of tRNA genes in the C. maculata mitogenome, which also include genes of plastid and bacterial origin in addition to their native mitochondrial counterparts.
publishDate 2025
dc.date.none.fl_str_mv 2025-01-30
dc.type.none.fl_str_mv info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
http://purl.org/coar/resource_type/c_6501
info:ar-repo/semantics/articulo
format article
status_str publishedVersion
dc.identifier.none.fl_str_mv http://hdl.handle.net/11336/282285
Warren, Jessica M.; Ceriotti, Luis Federico; Sánchez Puerta, María Virginia; Sloan, Daniel B.; Fungal-Derived tRNAs Are Expressed and Aminoacylated in Orchid Mitochondria; Oxford University Press; Molecular Biology and Evolution; 42; 2; 30-1-2025; 1-6
0737-4038
CONICET Digital
CONICET
url http://hdl.handle.net/11336/282285
identifier_str_mv Warren, Jessica M.; Ceriotti, Luis Federico; Sánchez Puerta, María Virginia; Sloan, Daniel B.; Fungal-Derived tRNAs Are Expressed and Aminoacylated in Orchid Mitochondria; Oxford University Press; Molecular Biology and Evolution; 42; 2; 30-1-2025; 1-6
0737-4038
CONICET Digital
CONICET
dc.language.none.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv info:eu-repo/semantics/altIdentifier/url/https://academic.oup.com/mbe/article/doi/10.1093/molbev/msaf025/7990429
info:eu-repo/semantics/altIdentifier/doi/10.1093/molbev/msaf025
dc.rights.none.fl_str_mv info:eu-repo/semantics/openAccess
https://creativecommons.org/licenses/by/2.5/ar/
eu_rights_str_mv openAccess
rights_invalid_str_mv https://creativecommons.org/licenses/by/2.5/ar/
dc.format.none.fl_str_mv application/pdf
application/pdf
application/pdf
dc.publisher.none.fl_str_mv Oxford University Press
publisher.none.fl_str_mv Oxford University Press
dc.source.none.fl_str_mv reponame:CONICET Digital (CONICET)
instname:Consejo Nacional de Investigaciones Científicas y Técnicas
reponame_str CONICET Digital (CONICET)
collection CONICET Digital (CONICET)
instname_str Consejo Nacional de Investigaciones Científicas y Técnicas
repository.name.fl_str_mv CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicas
repository.mail.fl_str_mv dasensio@conicet.gov.ar; lcarlino@conicet.gov.ar
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