Microfluidics-based encapsulation of isoniazid in egg white/carrageenan microparticles for sustained release

Autores
Marengo, Robinson Cristian; Mengatto, Luciano Nicolas; Olivares, María Laura; Berli, Claudio Luis Alberto
Año de publicación
2021
Idioma
inglés
Tipo de recurso
artículo
Estado
versión publicada
Descripción
The prevalence of tuberculosis continuously grows and there is an imperious need to improve the therapeutic efficacy of approved drugs for clinical use. Here we report the encapsulation of isoniazid (INH) in egg white/ - carrageenan microparticles, which are intended for drug vehiculation through the gastrointestinal tract and con- trolled release in the ileum. Spherical and highly monodisperse microparticles (255 mm average diameter) were obtained by droplet-based microfluidics and subsequent microwave irradiation. The entire amount of INH added to the particles was encapsulated. Infrared spectra revealed the formation of esters, hydrogen bonding, and Mail- lard reaction in the biopolymer matrix. In vitro release experiments were carried out in media that systematically emulate the stomach and intestinal tract conditions: 37 °C, NaCl 0.05 mol/L, pH 1.6, for the first 2 h, and Tris?HCl 0.1 mol/L, pH 7, for the next 24 h. A small fraction of the loaded INH was released in the first medium and most of the drug was progressively delivered in the second medium. The release profiles of microparticles were analyzed by using classical kinetic models, which enabled to hypothesize the release mechanism of INH from the biopolymer matrix. This knowledge, together with the ability to control the governing parameters of microfluidic elaboration, opens further possibilities for designing optimal prototypes for sustained release.
Fil: Marengo, Robinson Cristian. Universidad Nacional del Litoral; Argentina
Fil: Mengatto, Luciano Nicolas. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Santa Fe. Instituto de Desarrollo Tecnológico para la Industria Química. Universidad Nacional del Litoral. Instituto de Desarrollo Tecnológico para la Industria Química; Argentina
Fil: Olivares, María Laura. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Santa Fe. Instituto de Desarrollo Tecnológico para la Industria Química. Universidad Nacional del Litoral. Instituto de Desarrollo Tecnológico para la Industria Química; Argentina
Fil: Berli, Claudio Luis Alberto. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Santa Fe. Instituto de Desarrollo Tecnológico para la Industria Química. Universidad Nacional del Litoral. Instituto de Desarrollo Tecnológico para la Industria Química; Argentina
Materia
ISONIAZID
ENCAPSULATION
MICROFLUIDICS
DRUG RELEASE
Nivel de accesibilidad
acceso abierto
Condiciones de uso
https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
Repositorio
CONICET Digital (CONICET)
Institución
Consejo Nacional de Investigaciones Científicas y Técnicas
OAI Identificador
oai:ri.conicet.gov.ar:11336/150280
Acceder
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oai_identifier_str oai:ri.conicet.gov.ar:11336/150280
network_acronym_str CONICETDig
repository_id_str 3498
network_name_str CONICET Digital (CONICET)
spelling Microfluidics-based encapsulation of isoniazid in egg white/carrageenan microparticles for sustained releaseMarengo, Robinson CristianMengatto, Luciano NicolasOlivares, María LauraBerli, Claudio Luis AlbertoISONIAZIDENCAPSULATIONMICROFLUIDICSDRUG RELEASEhttps://purl.org/becyt/ford/2.5https://purl.org/becyt/ford/2The prevalence of tuberculosis continuously grows and there is an imperious need to improve the therapeutic efficacy of approved drugs for clinical use. Here we report the encapsulation of isoniazid (INH) in egg white/ - carrageenan microparticles, which are intended for drug vehiculation through the gastrointestinal tract and con- trolled release in the ileum. Spherical and highly monodisperse microparticles (255 mm average diameter) were obtained by droplet-based microfluidics and subsequent microwave irradiation. The entire amount of INH added to the particles was encapsulated. Infrared spectra revealed the formation of esters, hydrogen bonding, and Mail- lard reaction in the biopolymer matrix. In vitro release experiments were carried out in media that systematically emulate the stomach and intestinal tract conditions: 37 °C, NaCl 0.05 mol/L, pH 1.6, for the first 2 h, and Tris?HCl 0.1 mol/L, pH 7, for the next 24 h. A small fraction of the loaded INH was released in the first medium and most of the drug was progressively delivered in the second medium. The release profiles of microparticles were analyzed by using classical kinetic models, which enabled to hypothesize the release mechanism of INH from the biopolymer matrix. This knowledge, together with the ability to control the governing parameters of microfluidic elaboration, opens further possibilities for designing optimal prototypes for sustained release.Fil: Marengo, Robinson Cristian. Universidad Nacional del Litoral; ArgentinaFil: Mengatto, Luciano Nicolas. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Santa Fe. Instituto de Desarrollo Tecnológico para la Industria Química. Universidad Nacional del Litoral. Instituto de Desarrollo Tecnológico para la Industria Química; ArgentinaFil: Olivares, María Laura. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Santa Fe. Instituto de Desarrollo Tecnológico para la Industria Química. Universidad Nacional del Litoral. Instituto de Desarrollo Tecnológico para la Industria Química; ArgentinaFil: Berli, Claudio Luis Alberto. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Santa Fe. Instituto de Desarrollo Tecnológico para la Industria Química. Universidad Nacional del Litoral. Instituto de Desarrollo Tecnológico para la Industria Química; ArgentinaElsevier2021-11info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionhttp://purl.org/coar/resource_type/c_6501info:ar-repo/semantics/articuloapplication/pdfapplication/pdfhttp://hdl.handle.net/11336/150280Marengo, Robinson Cristian; Mengatto, Luciano Nicolas; Olivares, María Laura; Berli, Claudio Luis Alberto; Microfluidics-based encapsulation of isoniazid in egg white/carrageenan microparticles for sustained release; Elsevier; Food Hydrocolloids; 1; 11-2021; 1-82667-0259CONICET DigitalCONICETenginfo:eu-repo/semantics/altIdentifier/url/https://linkinghub.elsevier.com/retrieve/pii/S2667025921000339info:eu-repo/semantics/altIdentifier/doi/10.1016/j.fhfh.2021.100041info:eu-repo/semantics/openAccesshttps://creativecommons.org/licenses/by-nc-sa/2.5/ar/reponame:CONICET Digital (CONICET)instname:Consejo Nacional de Investigaciones Científicas y Técnicas2025-10-15T15:22:48Zoai:ri.conicet.gov.ar:11336/150280instacron:CONICETInstitucionalhttp://ri.conicet.gov.ar/Organismo científico-tecnológicoNo correspondehttp://ri.conicet.gov.ar/oai/requestdasensio@conicet.gov.ar; lcarlino@conicet.gov.arArgentinaNo correspondeNo correspondeNo correspondeopendoar:34982025-10-15 15:22:48.505CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicasfalse
dc.title.none.fl_str_mv Microfluidics-based encapsulation of isoniazid in egg white/carrageenan microparticles for sustained release
title Microfluidics-based encapsulation of isoniazid in egg white/carrageenan microparticles for sustained release
spellingShingle Microfluidics-based encapsulation of isoniazid in egg white/carrageenan microparticles for sustained release
Marengo, Robinson Cristian
ISONIAZID
ENCAPSULATION
MICROFLUIDICS
DRUG RELEASE
title_short Microfluidics-based encapsulation of isoniazid in egg white/carrageenan microparticles for sustained release
title_full Microfluidics-based encapsulation of isoniazid in egg white/carrageenan microparticles for sustained release
title_fullStr Microfluidics-based encapsulation of isoniazid in egg white/carrageenan microparticles for sustained release
title_full_unstemmed Microfluidics-based encapsulation of isoniazid in egg white/carrageenan microparticles for sustained release
title_sort Microfluidics-based encapsulation of isoniazid in egg white/carrageenan microparticles for sustained release
dc.creator.none.fl_str_mv Marengo, Robinson Cristian
Mengatto, Luciano Nicolas
Olivares, María Laura
Berli, Claudio Luis Alberto
author Marengo, Robinson Cristian
author_facet Marengo, Robinson Cristian
Mengatto, Luciano Nicolas
Olivares, María Laura
Berli, Claudio Luis Alberto
author_role author
author2 Mengatto, Luciano Nicolas
Olivares, María Laura
Berli, Claudio Luis Alberto
author2_role author
author
author
dc.subject.none.fl_str_mv ISONIAZID
ENCAPSULATION
MICROFLUIDICS
DRUG RELEASE
topic ISONIAZID
ENCAPSULATION
MICROFLUIDICS
DRUG RELEASE
purl_subject.fl_str_mv https://purl.org/becyt/ford/2.5
https://purl.org/becyt/ford/2
dc.description.none.fl_txt_mv The prevalence of tuberculosis continuously grows and there is an imperious need to improve the therapeutic efficacy of approved drugs for clinical use. Here we report the encapsulation of isoniazid (INH) in egg white/ - carrageenan microparticles, which are intended for drug vehiculation through the gastrointestinal tract and con- trolled release in the ileum. Spherical and highly monodisperse microparticles (255 mm average diameter) were obtained by droplet-based microfluidics and subsequent microwave irradiation. The entire amount of INH added to the particles was encapsulated. Infrared spectra revealed the formation of esters, hydrogen bonding, and Mail- lard reaction in the biopolymer matrix. In vitro release experiments were carried out in media that systematically emulate the stomach and intestinal tract conditions: 37 °C, NaCl 0.05 mol/L, pH 1.6, for the first 2 h, and Tris?HCl 0.1 mol/L, pH 7, for the next 24 h. A small fraction of the loaded INH was released in the first medium and most of the drug was progressively delivered in the second medium. The release profiles of microparticles were analyzed by using classical kinetic models, which enabled to hypothesize the release mechanism of INH from the biopolymer matrix. This knowledge, together with the ability to control the governing parameters of microfluidic elaboration, opens further possibilities for designing optimal prototypes for sustained release.
Fil: Marengo, Robinson Cristian. Universidad Nacional del Litoral; Argentina
Fil: Mengatto, Luciano Nicolas. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Santa Fe. Instituto de Desarrollo Tecnológico para la Industria Química. Universidad Nacional del Litoral. Instituto de Desarrollo Tecnológico para la Industria Química; Argentina
Fil: Olivares, María Laura. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Santa Fe. Instituto de Desarrollo Tecnológico para la Industria Química. Universidad Nacional del Litoral. Instituto de Desarrollo Tecnológico para la Industria Química; Argentina
Fil: Berli, Claudio Luis Alberto. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Santa Fe. Instituto de Desarrollo Tecnológico para la Industria Química. Universidad Nacional del Litoral. Instituto de Desarrollo Tecnológico para la Industria Química; Argentina
description The prevalence of tuberculosis continuously grows and there is an imperious need to improve the therapeutic efficacy of approved drugs for clinical use. Here we report the encapsulation of isoniazid (INH) in egg white/ - carrageenan microparticles, which are intended for drug vehiculation through the gastrointestinal tract and con- trolled release in the ileum. Spherical and highly monodisperse microparticles (255 mm average diameter) were obtained by droplet-based microfluidics and subsequent microwave irradiation. The entire amount of INH added to the particles was encapsulated. Infrared spectra revealed the formation of esters, hydrogen bonding, and Mail- lard reaction in the biopolymer matrix. In vitro release experiments were carried out in media that systematically emulate the stomach and intestinal tract conditions: 37 °C, NaCl 0.05 mol/L, pH 1.6, for the first 2 h, and Tris?HCl 0.1 mol/L, pH 7, for the next 24 h. A small fraction of the loaded INH was released in the first medium and most of the drug was progressively delivered in the second medium. The release profiles of microparticles were analyzed by using classical kinetic models, which enabled to hypothesize the release mechanism of INH from the biopolymer matrix. This knowledge, together with the ability to control the governing parameters of microfluidic elaboration, opens further possibilities for designing optimal prototypes for sustained release.
publishDate 2021
dc.date.none.fl_str_mv 2021-11
dc.type.none.fl_str_mv info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
http://purl.org/coar/resource_type/c_6501
info:ar-repo/semantics/articulo
format article
status_str publishedVersion
dc.identifier.none.fl_str_mv http://hdl.handle.net/11336/150280
Marengo, Robinson Cristian; Mengatto, Luciano Nicolas; Olivares, María Laura; Berli, Claudio Luis Alberto; Microfluidics-based encapsulation of isoniazid in egg white/carrageenan microparticles for sustained release; Elsevier; Food Hydrocolloids; 1; 11-2021; 1-8
2667-0259
CONICET Digital
CONICET
url http://hdl.handle.net/11336/150280
identifier_str_mv Marengo, Robinson Cristian; Mengatto, Luciano Nicolas; Olivares, María Laura; Berli, Claudio Luis Alberto; Microfluidics-based encapsulation of isoniazid in egg white/carrageenan microparticles for sustained release; Elsevier; Food Hydrocolloids; 1; 11-2021; 1-8
2667-0259
CONICET Digital
CONICET
dc.language.none.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv info:eu-repo/semantics/altIdentifier/url/https://linkinghub.elsevier.com/retrieve/pii/S2667025921000339
info:eu-repo/semantics/altIdentifier/doi/10.1016/j.fhfh.2021.100041
dc.rights.none.fl_str_mv info:eu-repo/semantics/openAccess
https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
eu_rights_str_mv openAccess
rights_invalid_str_mv https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
dc.format.none.fl_str_mv application/pdf
application/pdf
dc.publisher.none.fl_str_mv Elsevier
publisher.none.fl_str_mv Elsevier
dc.source.none.fl_str_mv reponame:CONICET Digital (CONICET)
instname:Consejo Nacional de Investigaciones Científicas y Técnicas
reponame_str CONICET Digital (CONICET)
collection CONICET Digital (CONICET)
instname_str Consejo Nacional de Investigaciones Científicas y Técnicas
repository.name.fl_str_mv CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicas
repository.mail.fl_str_mv dasensio@conicet.gov.ar; lcarlino@conicet.gov.ar
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score 13.22299

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