Biological and probiotic characterization of spontaneous phage-resistant mutants of Lactobacillus plantarum

Autores
Briggiler Marcó, Mariángeles; Zacarías, María Florencia; Vinderola, Celso Gabriel; Reinheimer, Jorge Alberto; Quiberoni, Andrea del Lujan
Año de publicación
2014
Idioma
inglés
Tipo de recurso
artículo
Estado
versión publicada
Descripción
Four spontaneous phage-resistant mutants, previously isolated from Lactobacillus plantarum ATCC 8014 using a phage cocktail (ATCC 8014-B1 and ATCC 8014-B2), were characterized about their probiotic potential. Phage-resistant mutants exhibited, in general, the same properties as those found for Lb. plantarum ATCC 8014 strain. However, mutant M1 evidenced a remarkably high resistance to gastrointestinal passage. Low values of either B-galactosidase activity or hydrophobicity were observed. Antimicrobial activity against Gram-negative and Gram-positive bacteria was evidenced and lactulose was the most fermented carbohydrate. Strains were sensitive to gentamicin, erythromycin, ampicillin and chloramphenicol, whereas production of biogenic amines was not observed. Finally, a selected phage-resistant mutant (M1) produced the same immunological response as the sensitive strain in mice fed for 10 consecutive days. These natural mutants, with similar or improved potentially probiotic characteristics regarding their sensitive strain, could be used during fermented food manufacture to minimize the phage attacks.
Fil: Briggiler Marcó, Mariángeles. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Santa Fe. Instituto de Lactología Industrial. Universidad Nacional del Litoral. Facultad de Ingeniería Química. Instituto de Lactología Industrial; Argentina
Fil: Zacarías, María Florencia. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Santa Fe. Instituto de Lactología Industrial. Universidad Nacional del Litoral. Facultad de Ingeniería Química. Instituto de Lactología Industrial; Argentina
Fil: Vinderola, Celso Gabriel. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Santa Fe. Instituto de Lactología Industrial. Universidad Nacional del Litoral. Facultad de Ingeniería Química. Instituto de Lactología Industrial; Argentina
Fil: Reinheimer, Jorge Alberto. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Santa Fe. Instituto de Lactología Industrial. Universidad Nacional del Litoral. Facultad de Ingeniería Química. Instituto de Lactología Industrial; Argentina
Fil: Quiberoni, Andrea del Lujan. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Santa Fe. Instituto de Lactología Industrial. Universidad Nacional del Litoral. Facultad de Ingeniería Química. Instituto de Lactología Industrial; Argentina
Materia
Lactobacillus Plantarum
Phage Cocktails
Phage-Resistant Mutants
Biological Resistance
Nivel de accesibilidad
acceso abierto
Condiciones de uso
https://creativecommons.org/licenses/by-nc-nd/2.5/ar/
Repositorio
CONICET Digital (CONICET)
Institución
Consejo Nacional de Investigaciones Científicas y Técnicas
OAI Identificador
oai:ri.conicet.gov.ar:11336/31035
Acceder
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oai_identifier_str oai:ri.conicet.gov.ar:11336/31035
network_acronym_str CONICETDig
repository_id_str 3498
network_name_str CONICET Digital (CONICET)
spelling Biological and probiotic characterization of spontaneous phage-resistant mutants of Lactobacillus plantarumBriggiler Marcó, MariángelesZacarías, María FlorenciaVinderola, Celso GabrielReinheimer, Jorge AlbertoQuiberoni, Andrea del LujanLactobacillus PlantarumPhage CocktailsPhage-Resistant MutantsBiological Resistancehttps://purl.org/becyt/ford/2.11https://purl.org/becyt/ford/2Four spontaneous phage-resistant mutants, previously isolated from Lactobacillus plantarum ATCC 8014 using a phage cocktail (ATCC 8014-B1 and ATCC 8014-B2), were characterized about their probiotic potential. Phage-resistant mutants exhibited, in general, the same properties as those found for Lb. plantarum ATCC 8014 strain. However, mutant M1 evidenced a remarkably high resistance to gastrointestinal passage. Low values of either B-galactosidase activity or hydrophobicity were observed. Antimicrobial activity against Gram-negative and Gram-positive bacteria was evidenced and lactulose was the most fermented carbohydrate. Strains were sensitive to gentamicin, erythromycin, ampicillin and chloramphenicol, whereas production of biogenic amines was not observed. Finally, a selected phage-resistant mutant (M1) produced the same immunological response as the sensitive strain in mice fed for 10 consecutive days. These natural mutants, with similar or improved potentially probiotic characteristics regarding their sensitive strain, could be used during fermented food manufacture to minimize the phage attacks.Fil: Briggiler Marcó, Mariángeles. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Santa Fe. Instituto de Lactología Industrial. Universidad Nacional del Litoral. Facultad de Ingeniería Química. Instituto de Lactología Industrial; ArgentinaFil: Zacarías, María Florencia. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Santa Fe. Instituto de Lactología Industrial. Universidad Nacional del Litoral. Facultad de Ingeniería Química. Instituto de Lactología Industrial; ArgentinaFil: Vinderola, Celso Gabriel. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Santa Fe. Instituto de Lactología Industrial. Universidad Nacional del Litoral. Facultad de Ingeniería Química. Instituto de Lactología Industrial; ArgentinaFil: Reinheimer, Jorge Alberto. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Santa Fe. Instituto de Lactología Industrial. Universidad Nacional del Litoral. Facultad de Ingeniería Química. Instituto de Lactología Industrial; ArgentinaFil: Quiberoni, Andrea del Lujan. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Santa Fe. Instituto de Lactología Industrial. Universidad Nacional del Litoral. Facultad de Ingeniería Química. Instituto de Lactología Industrial; ArgentinaElsevier2014-06info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionhttp://purl.org/coar/resource_type/c_6501info:ar-repo/semantics/articuloapplication/pdfapplication/pdfapplication/pdfapplication/pdfapplication/pdfhttp://hdl.handle.net/11336/31035Quiberoni, Andrea del Lujan; Reinheimer, Jorge Alberto; Vinderola, Celso Gabriel; Zacarías, María Florencia; Briggiler Marcó, Mariángeles; Biological and probiotic characterization of spontaneous phage-resistant mutants of Lactobacillus plantarum; Elsevier; International Dairy Journal; 39; 1; 6-2014; 64-700958-6946CONICET DigitalCONICETenginfo:eu-repo/semantics/altIdentifier/url/http://www.sciencedirect.com/science/article/pii/S0958694614001162info:eu-repo/semantics/altIdentifier/doi/10.1016/j.idairyj.2014.05.012info:eu-repo/semantics/openAccesshttps://creativecommons.org/licenses/by-nc-nd/2.5/ar/reponame:CONICET Digital (CONICET)instname:Consejo Nacional de Investigaciones Científicas y Técnicas2025-10-29T11:17:23Zoai:ri.conicet.gov.ar:11336/31035instacron:CONICETInstitucionalhttp://ri.conicet.gov.ar/Organismo científico-tecnológicoNo correspondehttp://ri.conicet.gov.ar/oai/requestdasensio@conicet.gov.ar; lcarlino@conicet.gov.arArgentinaNo correspondeNo correspondeNo correspondeopendoar:34982025-10-29 11:17:23.614CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicasfalse
dc.title.none.fl_str_mv Biological and probiotic characterization of spontaneous phage-resistant mutants of Lactobacillus plantarum
title Biological and probiotic characterization of spontaneous phage-resistant mutants of Lactobacillus plantarum
spellingShingle Biological and probiotic characterization of spontaneous phage-resistant mutants of Lactobacillus plantarum
Briggiler Marcó, Mariángeles
Lactobacillus Plantarum
Phage Cocktails
Phage-Resistant Mutants
Biological Resistance
title_short Biological and probiotic characterization of spontaneous phage-resistant mutants of Lactobacillus plantarum
title_full Biological and probiotic characterization of spontaneous phage-resistant mutants of Lactobacillus plantarum
title_fullStr Biological and probiotic characterization of spontaneous phage-resistant mutants of Lactobacillus plantarum
title_full_unstemmed Biological and probiotic characterization of spontaneous phage-resistant mutants of Lactobacillus plantarum
title_sort Biological and probiotic characterization of spontaneous phage-resistant mutants of Lactobacillus plantarum
dc.creator.none.fl_str_mv Briggiler Marcó, Mariángeles
Zacarías, María Florencia
Vinderola, Celso Gabriel
Reinheimer, Jorge Alberto
Quiberoni, Andrea del Lujan
author Briggiler Marcó, Mariángeles
author_facet Briggiler Marcó, Mariángeles
Zacarías, María Florencia
Vinderola, Celso Gabriel
Reinheimer, Jorge Alberto
Quiberoni, Andrea del Lujan
author_role author
author2 Zacarías, María Florencia
Vinderola, Celso Gabriel
Reinheimer, Jorge Alberto
Quiberoni, Andrea del Lujan
author2_role author
author
author
author
dc.subject.none.fl_str_mv Lactobacillus Plantarum
Phage Cocktails
Phage-Resistant Mutants
Biological Resistance
topic Lactobacillus Plantarum
Phage Cocktails
Phage-Resistant Mutants
Biological Resistance
purl_subject.fl_str_mv https://purl.org/becyt/ford/2.11
https://purl.org/becyt/ford/2
dc.description.none.fl_txt_mv Four spontaneous phage-resistant mutants, previously isolated from Lactobacillus plantarum ATCC 8014 using a phage cocktail (ATCC 8014-B1 and ATCC 8014-B2), were characterized about their probiotic potential. Phage-resistant mutants exhibited, in general, the same properties as those found for Lb. plantarum ATCC 8014 strain. However, mutant M1 evidenced a remarkably high resistance to gastrointestinal passage. Low values of either B-galactosidase activity or hydrophobicity were observed. Antimicrobial activity against Gram-negative and Gram-positive bacteria was evidenced and lactulose was the most fermented carbohydrate. Strains were sensitive to gentamicin, erythromycin, ampicillin and chloramphenicol, whereas production of biogenic amines was not observed. Finally, a selected phage-resistant mutant (M1) produced the same immunological response as the sensitive strain in mice fed for 10 consecutive days. These natural mutants, with similar or improved potentially probiotic characteristics regarding their sensitive strain, could be used during fermented food manufacture to minimize the phage attacks.
Fil: Briggiler Marcó, Mariángeles. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Santa Fe. Instituto de Lactología Industrial. Universidad Nacional del Litoral. Facultad de Ingeniería Química. Instituto de Lactología Industrial; Argentina
Fil: Zacarías, María Florencia. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Santa Fe. Instituto de Lactología Industrial. Universidad Nacional del Litoral. Facultad de Ingeniería Química. Instituto de Lactología Industrial; Argentina
Fil: Vinderola, Celso Gabriel. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Santa Fe. Instituto de Lactología Industrial. Universidad Nacional del Litoral. Facultad de Ingeniería Química. Instituto de Lactología Industrial; Argentina
Fil: Reinheimer, Jorge Alberto. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Santa Fe. Instituto de Lactología Industrial. Universidad Nacional del Litoral. Facultad de Ingeniería Química. Instituto de Lactología Industrial; Argentina
Fil: Quiberoni, Andrea del Lujan. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Santa Fe. Instituto de Lactología Industrial. Universidad Nacional del Litoral. Facultad de Ingeniería Química. Instituto de Lactología Industrial; Argentina
description Four spontaneous phage-resistant mutants, previously isolated from Lactobacillus plantarum ATCC 8014 using a phage cocktail (ATCC 8014-B1 and ATCC 8014-B2), were characterized about their probiotic potential. Phage-resistant mutants exhibited, in general, the same properties as those found for Lb. plantarum ATCC 8014 strain. However, mutant M1 evidenced a remarkably high resistance to gastrointestinal passage. Low values of either B-galactosidase activity or hydrophobicity were observed. Antimicrobial activity against Gram-negative and Gram-positive bacteria was evidenced and lactulose was the most fermented carbohydrate. Strains were sensitive to gentamicin, erythromycin, ampicillin and chloramphenicol, whereas production of biogenic amines was not observed. Finally, a selected phage-resistant mutant (M1) produced the same immunological response as the sensitive strain in mice fed for 10 consecutive days. These natural mutants, with similar or improved potentially probiotic characteristics regarding their sensitive strain, could be used during fermented food manufacture to minimize the phage attacks.
publishDate 2014
dc.date.none.fl_str_mv 2014-06
dc.type.none.fl_str_mv info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
http://purl.org/coar/resource_type/c_6501
info:ar-repo/semantics/articulo
format article
status_str publishedVersion
dc.identifier.none.fl_str_mv http://hdl.handle.net/11336/31035
Quiberoni, Andrea del Lujan; Reinheimer, Jorge Alberto; Vinderola, Celso Gabriel; Zacarías, María Florencia; Briggiler Marcó, Mariángeles; Biological and probiotic characterization of spontaneous phage-resistant mutants of Lactobacillus plantarum; Elsevier; International Dairy Journal; 39; 1; 6-2014; 64-70
0958-6946
CONICET Digital
CONICET
url http://hdl.handle.net/11336/31035
identifier_str_mv Quiberoni, Andrea del Lujan; Reinheimer, Jorge Alberto; Vinderola, Celso Gabriel; Zacarías, María Florencia; Briggiler Marcó, Mariángeles; Biological and probiotic characterization of spontaneous phage-resistant mutants of Lactobacillus plantarum; Elsevier; International Dairy Journal; 39; 1; 6-2014; 64-70
0958-6946
CONICET Digital
CONICET
dc.language.none.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv info:eu-repo/semantics/altIdentifier/url/http://www.sciencedirect.com/science/article/pii/S0958694614001162
info:eu-repo/semantics/altIdentifier/doi/10.1016/j.idairyj.2014.05.012
dc.rights.none.fl_str_mv info:eu-repo/semantics/openAccess
https://creativecommons.org/licenses/by-nc-nd/2.5/ar/
eu_rights_str_mv openAccess
rights_invalid_str_mv https://creativecommons.org/licenses/by-nc-nd/2.5/ar/
dc.format.none.fl_str_mv application/pdf
application/pdf
application/pdf
application/pdf
application/pdf
dc.publisher.none.fl_str_mv Elsevier
publisher.none.fl_str_mv Elsevier
dc.source.none.fl_str_mv reponame:CONICET Digital (CONICET)
instname:Consejo Nacional de Investigaciones Científicas y Técnicas
reponame_str CONICET Digital (CONICET)
collection CONICET Digital (CONICET)
instname_str Consejo Nacional de Investigaciones Científicas y Técnicas
repository.name.fl_str_mv CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicas
repository.mail.fl_str_mv dasensio@conicet.gov.ar; lcarlino@conicet.gov.ar
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score 13.10058

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