Flow Injection-Traveling-Wave Ion Mobility-Mass Spectrometry for Prostate-Cancer Metabolomics

• Autores: Zang, Xiaoling; Monge, Maria Eugenia; Gaul, David A.; Fernandez, Facundo M.
• Año de publicación: 2018
• Idioma: inglés
• Tipo de recurso: artículo
• Materia: MASS SPECTROMETRY; ION MOBILITY; FLOW INJECTION; PROSTATE CANCER
• Institución: Consejo Nacional de Investigaciones Científicas y Técnicas

Flow injection-traveling-wave ion mobility-mass spectrometry (FITWIM-MS) was applied to the nontargeted metabolic profiling of serum extracts from 61 prostate-cancer (PCa) patients and 42 controls with an analysis speed of 6 min per sample, including a 3 min wash run. Comprehensive data mining of the mobility-mass domain was used to discriminate species with various charge states and filter matrix saltcluster ions. Specific criteria were developed to ensure correct grouping of adducts, insource fragments, and impurities in the data set. Endogenous metabolites were identified with high confidence using FI-TWIM-MS/MS and collision-cross-section (CCS) matching with chemical standards or CCS databases. PCa patient samples were distinguished from control samples with good accuracies (88.3-89.3%), sensitivities (88.5-90.2%), and specificity (88.1%) using supervised multivariate classification methods. Although largely underutilized in metabolomics studies, FI-TWIM-MS proved advantageous in terms of analysis speed, separation of ions in complex mixtures, improved signal-to-noise ratio, and reduction of spectral congestion. Results from this study showcase the potential of FITWIM-MS as a high-throughput metabolic-profiling tool for large-scale metabolomics studies.
Fil: Zang, Xiaoling. Georgia Institute of Techology; Estados Unidos
Fil: Monge, Maria Eugenia. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Parque Centenario. Centro de Investigaciones en Bionanociencias "Elizabeth Jares Erijman"; Argentina
Fil: Gaul, David A.. Georgia Institute of Techology; Estados Unidos
Fil: Fernandez, Facundo M.. Georgia Institute of Techology; Estados Unidos