Challenging the phylogenetic relationships among Echinococcus multilocularis isolates from main endemic areas

Autores
Lallemand, Séverine; Oyhenart, Jorge; Valot, Benoit; Borne, Romain; Bohard, Louis; Umhang, Gérald; Karamon, Jacek; Konyaev, Sergey; Rönnberg, Caroline; Gottstein, Bruno; Weil Verhoeven, Delphine; Richou, Carine; Bresson Hadni, Solange; Millon, Laurence; Bellanger, Anne-Pauline; Knapp, Jenny
Año de publicación
2024
Idioma
inglés
Tipo de recurso
artículo
Estado
versión publicada
Descripción
Alveolar echinococcosis (AE) is a rare but severe disease that affects more than 18,000 people worldwide per year. The complete sequencing of the mitochondrial genome of Echinococcus multilocularis has made it possible to study the genetic diversity of the parasite and its spatial and temporal evolution. We amplified the whole mitochondrial genome by PCR, using one uniplex and two multiplex reactions to cover the 13,738 bp of the mitogenome, and then sequenced the amplicons with Illumina technology. In total, 113 samples from Europe, Asia, the Arctic and North America were analyzed. Three major haplogroups were found: HG1, which clustered samples from Alaska (including Saint-Lawrence Island), Yakutia (Russia) and Svalbard; HG2, with samples from Asia, North America and Europe; and HG3, subdivided into three micro-haplogroups. HG3a included samples from North America and Europe, whereas HG3b and HG3c only include samples from Europe. In France, HG3a included samples from patients more recently diagnosed in a region outside the historical endemic area. A fourth putative haplogroup, HG4, was represented by only one isolate from Olkhon Island (Russia). The increased discriminatory power of the complete sequencing of the E. multilocularis mitogenome has made it possible to highlight four distinct geographical clusters, one being divided into three micro-haplogroups in France.
Fil: Lallemand, Séverine. Universite de Franche-Comte; Francia
Fil: Oyhenart, Jorge. Consejo Nacional de Investigaciones Científicas y Técnicas. Instituto de Ciencias de la Tierra y Ambientales de La Pampa. Universidad Nacional de La Pampa. Facultad de Ciencias Exactas y Naturales. Instituto de Ciencias de la Tierra y Ambientales de La Pampa; Argentina
Fil: Valot, Benoit. Universite de Franche-Comte; Francia
Fil: Borne, Romain. Universite de Franche-Comte; Francia
Fil: Bohard, Louis. University Hospital of Besançon; Francia
Fil: Umhang, Gérald. Rabies and Wildlife Laboratory; Francia
Fil: Karamon, Jacek. National Veterinary Research Institute; Polonia
Fil: Konyaev, Sergey. Institute of Systematics and Ecology of Animals; Rusia
Fil: Rönnberg, Caroline. Public Health Agency Of Sweden; Suecia
Fil: Gottstein, Bruno. University of Bern; Suiza
Fil: Weil Verhoeven, Delphine. University Hospital of Besançon; Francia
Fil: Richou, Carine. University Hospital of Besançon; Francia
Fil: Bresson Hadni, Solange. University Hospital of Besançon; Francia. Universite de Franche-Comte; Francia
Fil: Millon, Laurence. Universite de Franche-Comte; Francia. University Hospital of Besançon; Francia
Fil: Bellanger, Anne-Pauline. Universite de Franche-Comte; Francia. University Hospital of Besançon; Francia
Fil: Knapp, Jenny. Universite de Franche-Comte; Francia. University Hospital of Besançon; Francia
Materia
Echinococcus multilocularis
Whole mitogenome sequencing
Haplogroup
Phylogenetic inference
Nivel de accesibilidad
acceso abierto
Condiciones de uso
https://creativecommons.org/licenses/by-nc-nd/2.5/ar/
Repositorio
CONICET Digital (CONICET)
Institución
Consejo Nacional de Investigaciones Científicas y Técnicas
OAI Identificador
oai:ri.conicet.gov.ar:11336/266112
Acceder
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oai_identifier_str oai:ri.conicet.gov.ar:11336/266112
network_acronym_str CONICETDig
repository_id_str 3498
network_name_str CONICET Digital (CONICET)
spelling Challenging the phylogenetic relationships among Echinococcus multilocularis isolates from main endemic areasLallemand, SéverineOyhenart, JorgeValot, BenoitBorne, RomainBohard, LouisUmhang, GéraldKaramon, JacekKonyaev, SergeyRönnberg, CarolineGottstein, BrunoWeil Verhoeven, DelphineRichou, CarineBresson Hadni, SolangeMillon, LaurenceBellanger, Anne-PaulineKnapp, JennyEchinococcus multilocularisWhole mitogenome sequencingHaplogroupPhylogenetic inferencehttps://purl.org/becyt/ford/3.3https://purl.org/becyt/ford/3Alveolar echinococcosis (AE) is a rare but severe disease that affects more than 18,000 people worldwide per year. The complete sequencing of the mitochondrial genome of Echinococcus multilocularis has made it possible to study the genetic diversity of the parasite and its spatial and temporal evolution. We amplified the whole mitochondrial genome by PCR, using one uniplex and two multiplex reactions to cover the 13,738 bp of the mitogenome, and then sequenced the amplicons with Illumina technology. In total, 113 samples from Europe, Asia, the Arctic and North America were analyzed. Three major haplogroups were found: HG1, which clustered samples from Alaska (including Saint-Lawrence Island), Yakutia (Russia) and Svalbard; HG2, with samples from Asia, North America and Europe; and HG3, subdivided into three micro-haplogroups. HG3a included samples from North America and Europe, whereas HG3b and HG3c only include samples from Europe. In France, HG3a included samples from patients more recently diagnosed in a region outside the historical endemic area. A fourth putative haplogroup, HG4, was represented by only one isolate from Olkhon Island (Russia). The increased discriminatory power of the complete sequencing of the E. multilocularis mitogenome has made it possible to highlight four distinct geographical clusters, one being divided into three micro-haplogroups in France.Fil: Lallemand, Séverine. Universite de Franche-Comte; FranciaFil: Oyhenart, Jorge. Consejo Nacional de Investigaciones Científicas y Técnicas. Instituto de Ciencias de la Tierra y Ambientales de La Pampa. Universidad Nacional de La Pampa. Facultad de Ciencias Exactas y Naturales. Instituto de Ciencias de la Tierra y Ambientales de La Pampa; ArgentinaFil: Valot, Benoit. Universite de Franche-Comte; FranciaFil: Borne, Romain. Universite de Franche-Comte; FranciaFil: Bohard, Louis. University Hospital of Besançon; FranciaFil: Umhang, Gérald. Rabies and Wildlife Laboratory; FranciaFil: Karamon, Jacek. National Veterinary Research Institute; PoloniaFil: Konyaev, Sergey. Institute of Systematics and Ecology of Animals; RusiaFil: Rönnberg, Caroline. Public Health Agency Of Sweden; SueciaFil: Gottstein, Bruno. University of Bern; SuizaFil: Weil Verhoeven, Delphine. University Hospital of Besançon; FranciaFil: Richou, Carine. University Hospital of Besançon; FranciaFil: Bresson Hadni, Solange. University Hospital of Besançon; Francia. Universite de Franche-Comte; FranciaFil: Millon, Laurence. Universite de Franche-Comte; Francia. University Hospital of Besançon; FranciaFil: Bellanger, Anne-Pauline. Universite de Franche-Comte; Francia. University Hospital of Besançon; FranciaFil: Knapp, Jenny. Universite de Franche-Comte; Francia. University Hospital of Besançon; FranciaElsevier2024-09info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionhttp://purl.org/coar/resource_type/c_6501info:ar-repo/semantics/articuloapplication/pdfapplication/pdfapplication/pdfhttp://hdl.handle.net/11336/266112Lallemand, Séverine; Oyhenart, Jorge; Valot, Benoit; Borne, Romain; Bohard, Louis; et al.; Challenging the phylogenetic relationships among Echinococcus multilocularis isolates from main endemic areas; Elsevier; International Journal for Parasitology; 54; 11; 9-2024; 569-5820020-7519CONICET DigitalCONICETenginfo:eu-repo/semantics/altIdentifier/url/https://www.sciencedirect.com/science/article/pii/S0020751924001103info:eu-repo/semantics/altIdentifier/doi/10.1016/j.ijpara.2024.05.004info:eu-repo/semantics/openAccesshttps://creativecommons.org/licenses/by-nc-nd/2.5/ar/reponame:CONICET Digital (CONICET)instname:Consejo Nacional de Investigaciones Científicas y Técnicas2025-09-29T10:40:27Zoai:ri.conicet.gov.ar:11336/266112instacron:CONICETInstitucionalhttp://ri.conicet.gov.ar/Organismo científico-tecnológicoNo correspondehttp://ri.conicet.gov.ar/oai/requestdasensio@conicet.gov.ar; lcarlino@conicet.gov.arArgentinaNo correspondeNo correspondeNo correspondeopendoar:34982025-09-29 10:40:28.177CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicasfalse
dc.title.none.fl_str_mv Challenging the phylogenetic relationships among Echinococcus multilocularis isolates from main endemic areas
title Challenging the phylogenetic relationships among Echinococcus multilocularis isolates from main endemic areas
spellingShingle Challenging the phylogenetic relationships among Echinococcus multilocularis isolates from main endemic areas
Lallemand, Séverine
Echinococcus multilocularis
Whole mitogenome sequencing
Haplogroup
Phylogenetic inference
title_short Challenging the phylogenetic relationships among Echinococcus multilocularis isolates from main endemic areas
title_full Challenging the phylogenetic relationships among Echinococcus multilocularis isolates from main endemic areas
title_fullStr Challenging the phylogenetic relationships among Echinococcus multilocularis isolates from main endemic areas
title_full_unstemmed Challenging the phylogenetic relationships among Echinococcus multilocularis isolates from main endemic areas
title_sort Challenging the phylogenetic relationships among Echinococcus multilocularis isolates from main endemic areas
dc.creator.none.fl_str_mv Lallemand, Séverine
Oyhenart, Jorge
Valot, Benoit
Borne, Romain
Bohard, Louis
Umhang, Gérald
Karamon, Jacek
Konyaev, Sergey
Rönnberg, Caroline
Gottstein, Bruno
Weil Verhoeven, Delphine
Richou, Carine
Bresson Hadni, Solange
Millon, Laurence
Bellanger, Anne-Pauline
Knapp, Jenny
author Lallemand, Séverine
author_facet Lallemand, Séverine
Oyhenart, Jorge
Valot, Benoit
Borne, Romain
Bohard, Louis
Umhang, Gérald
Karamon, Jacek
Konyaev, Sergey
Rönnberg, Caroline
Gottstein, Bruno
Weil Verhoeven, Delphine
Richou, Carine
Bresson Hadni, Solange
Millon, Laurence
Bellanger, Anne-Pauline
Knapp, Jenny
author_role author
author2 Oyhenart, Jorge
Valot, Benoit
Borne, Romain
Bohard, Louis
Umhang, Gérald
Karamon, Jacek
Konyaev, Sergey
Rönnberg, Caroline
Gottstein, Bruno
Weil Verhoeven, Delphine
Richou, Carine
Bresson Hadni, Solange
Millon, Laurence
Bellanger, Anne-Pauline
Knapp, Jenny
author2_role author
author
author
author
author
author
author
author
author
author
author
author
author
author
author
dc.subject.none.fl_str_mv Echinococcus multilocularis
Whole mitogenome sequencing
Haplogroup
Phylogenetic inference
topic Echinococcus multilocularis
Whole mitogenome sequencing
Haplogroup
Phylogenetic inference
purl_subject.fl_str_mv https://purl.org/becyt/ford/3.3
https://purl.org/becyt/ford/3
dc.description.none.fl_txt_mv Alveolar echinococcosis (AE) is a rare but severe disease that affects more than 18,000 people worldwide per year. The complete sequencing of the mitochondrial genome of Echinococcus multilocularis has made it possible to study the genetic diversity of the parasite and its spatial and temporal evolution. We amplified the whole mitochondrial genome by PCR, using one uniplex and two multiplex reactions to cover the 13,738 bp of the mitogenome, and then sequenced the amplicons with Illumina technology. In total, 113 samples from Europe, Asia, the Arctic and North America were analyzed. Three major haplogroups were found: HG1, which clustered samples from Alaska (including Saint-Lawrence Island), Yakutia (Russia) and Svalbard; HG2, with samples from Asia, North America and Europe; and HG3, subdivided into three micro-haplogroups. HG3a included samples from North America and Europe, whereas HG3b and HG3c only include samples from Europe. In France, HG3a included samples from patients more recently diagnosed in a region outside the historical endemic area. A fourth putative haplogroup, HG4, was represented by only one isolate from Olkhon Island (Russia). The increased discriminatory power of the complete sequencing of the E. multilocularis mitogenome has made it possible to highlight four distinct geographical clusters, one being divided into three micro-haplogroups in France.
Fil: Lallemand, Séverine. Universite de Franche-Comte; Francia
Fil: Oyhenart, Jorge. Consejo Nacional de Investigaciones Científicas y Técnicas. Instituto de Ciencias de la Tierra y Ambientales de La Pampa. Universidad Nacional de La Pampa. Facultad de Ciencias Exactas y Naturales. Instituto de Ciencias de la Tierra y Ambientales de La Pampa; Argentina
Fil: Valot, Benoit. Universite de Franche-Comte; Francia
Fil: Borne, Romain. Universite de Franche-Comte; Francia
Fil: Bohard, Louis. University Hospital of Besançon; Francia
Fil: Umhang, Gérald. Rabies and Wildlife Laboratory; Francia
Fil: Karamon, Jacek. National Veterinary Research Institute; Polonia
Fil: Konyaev, Sergey. Institute of Systematics and Ecology of Animals; Rusia
Fil: Rönnberg, Caroline. Public Health Agency Of Sweden; Suecia
Fil: Gottstein, Bruno. University of Bern; Suiza
Fil: Weil Verhoeven, Delphine. University Hospital of Besançon; Francia
Fil: Richou, Carine. University Hospital of Besançon; Francia
Fil: Bresson Hadni, Solange. University Hospital of Besançon; Francia. Universite de Franche-Comte; Francia
Fil: Millon, Laurence. Universite de Franche-Comte; Francia. University Hospital of Besançon; Francia
Fil: Bellanger, Anne-Pauline. Universite de Franche-Comte; Francia. University Hospital of Besançon; Francia
Fil: Knapp, Jenny. Universite de Franche-Comte; Francia. University Hospital of Besançon; Francia
description Alveolar echinococcosis (AE) is a rare but severe disease that affects more than 18,000 people worldwide per year. The complete sequencing of the mitochondrial genome of Echinococcus multilocularis has made it possible to study the genetic diversity of the parasite and its spatial and temporal evolution. We amplified the whole mitochondrial genome by PCR, using one uniplex and two multiplex reactions to cover the 13,738 bp of the mitogenome, and then sequenced the amplicons with Illumina technology. In total, 113 samples from Europe, Asia, the Arctic and North America were analyzed. Three major haplogroups were found: HG1, which clustered samples from Alaska (including Saint-Lawrence Island), Yakutia (Russia) and Svalbard; HG2, with samples from Asia, North America and Europe; and HG3, subdivided into three micro-haplogroups. HG3a included samples from North America and Europe, whereas HG3b and HG3c only include samples from Europe. In France, HG3a included samples from patients more recently diagnosed in a region outside the historical endemic area. A fourth putative haplogroup, HG4, was represented by only one isolate from Olkhon Island (Russia). The increased discriminatory power of the complete sequencing of the E. multilocularis mitogenome has made it possible to highlight four distinct geographical clusters, one being divided into three micro-haplogroups in France.
publishDate 2024
dc.date.none.fl_str_mv 2024-09
dc.type.none.fl_str_mv info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
http://purl.org/coar/resource_type/c_6501
info:ar-repo/semantics/articulo
format article
status_str publishedVersion
dc.identifier.none.fl_str_mv http://hdl.handle.net/11336/266112
Lallemand, Séverine; Oyhenart, Jorge; Valot, Benoit; Borne, Romain; Bohard, Louis; et al.; Challenging the phylogenetic relationships among Echinococcus multilocularis isolates from main endemic areas; Elsevier; International Journal for Parasitology; 54; 11; 9-2024; 569-582
0020-7519
CONICET Digital
CONICET
url http://hdl.handle.net/11336/266112
identifier_str_mv Lallemand, Séverine; Oyhenart, Jorge; Valot, Benoit; Borne, Romain; Bohard, Louis; et al.; Challenging the phylogenetic relationships among Echinococcus multilocularis isolates from main endemic areas; Elsevier; International Journal for Parasitology; 54; 11; 9-2024; 569-582
0020-7519
CONICET Digital
CONICET
dc.language.none.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv info:eu-repo/semantics/altIdentifier/url/https://www.sciencedirect.com/science/article/pii/S0020751924001103
info:eu-repo/semantics/altIdentifier/doi/10.1016/j.ijpara.2024.05.004
dc.rights.none.fl_str_mv info:eu-repo/semantics/openAccess
https://creativecommons.org/licenses/by-nc-nd/2.5/ar/
eu_rights_str_mv openAccess
rights_invalid_str_mv https://creativecommons.org/licenses/by-nc-nd/2.5/ar/
dc.format.none.fl_str_mv application/pdf
application/pdf
application/pdf
dc.publisher.none.fl_str_mv Elsevier
publisher.none.fl_str_mv Elsevier
dc.source.none.fl_str_mv reponame:CONICET Digital (CONICET)
instname:Consejo Nacional de Investigaciones Científicas y Técnicas
reponame_str CONICET Digital (CONICET)
collection CONICET Digital (CONICET)
instname_str Consejo Nacional de Investigaciones Científicas y Técnicas
repository.name.fl_str_mv CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicas
repository.mail.fl_str_mv dasensio@conicet.gov.ar; lcarlino@conicet.gov.ar
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score 13.070432

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