Genome-wide analysis of DNA methylation in human amnion

Autores
Kim, Jinsil; Pitlick, Mitchell M.; Christine, Paul J.; Schaefer, Amanda R.; Saleme, Cesar; Comas, Belén; Cosentino, Viviana Raquel; Gadow, Enrique Curt; Murray, Jeffrey C.
Año de publicación
2013
Idioma
inglés
Tipo de recurso
artículo
Estado
versión publicada
Descripción
The amnion is a specialized tissue in contact with the amniotic fluid, which is in a constantly changing state. To investigate the importance of epigenetic events in this tissue in the physiology and pathophysiology of pregnancy, we performed genome-wide DNA methylation profiling of human amnion from term (with and without labor) and preterm deliveries. Using the Illumina Infinium HumanMethylation27 BeadChip, we identified genes exhibiting differential methylation associated with normal labor and preterm birth. Functional analysis of the differentially methylated genes revealed biologically relevant enriched gene sets. Bisulfite sequencing analysis of the promoter region of the oxytocin receptor (OXTR) gene detected two CpG dinucleotides showing significant methylation differences among the three groups of samples. Hypermethylation of the CpG island of the solute carrier family 30 member 3 (SLC30A3) gene in preterm amnion was confirmed by methylation-specific PCR. This work provides preliminary evidence that DNA methylation changes in the amnion may be at least partially involved in the physiological process of labor and the etiology of preterm birth and suggests that DNA methylation profiles, in combination with other biological data, may provide valuable insight into the mechanisms underlying normal and pathological pregnancies. © 2013 Jinsil Kim et al.
Fil: Kim, Jinsil. University of Iowa; Estados Unidos
Fil: Pitlick, Mitchell M.. University of Iowa; Estados Unidos
Fil: Christine, Paul J.. University of Iowa; Estados Unidos
Fil: Schaefer, Amanda R.. University of Iowa; Estados Unidos
Fil: Saleme, Cesar. Instituto de Maternidad y Ginecología Nuestra Señora de las Mercedes; Argentina
Fil: Comas, Belén. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina. Centro de Educación Médica e Investigaciones Clínicas “Norberto Quirno”; Argentina
Fil: Cosentino, Viviana Raquel. Centro de Educación Médica e Investigaciones Clínicas “Norberto Quirno”; Argentina
Fil: Gadow, Enrique Curt. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina. Centro de Educación Médica e Investigaciones Clínicas “Norberto Quirno”; Argentina
Fil: Murray, Jeffrey C.. University of Iowa; Estados Unidos
Materia
preterm birth
genome-wide DNA methylation
human placenta
Nivel de accesibilidad
acceso abierto
Condiciones de uso
https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
Repositorio
CONICET Digital (CONICET)
Institución
Consejo Nacional de Investigaciones Científicas y Técnicas
OAI Identificador
oai:ri.conicet.gov.ar:11336/77775
Acceder
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network_name_str CONICET Digital (CONICET)
spelling Genome-wide analysis of DNA methylation in human amnionKim, JinsilPitlick, Mitchell M.Christine, Paul J.Schaefer, Amanda R.Saleme, CesarComas, BelénCosentino, Viviana RaquelGadow, Enrique CurtMurray, Jeffrey C.preterm birthgenome-wide DNA methylationhuman placentahttps://purl.org/becyt/ford/3.1https://purl.org/becyt/ford/3The amnion is a specialized tissue in contact with the amniotic fluid, which is in a constantly changing state. To investigate the importance of epigenetic events in this tissue in the physiology and pathophysiology of pregnancy, we performed genome-wide DNA methylation profiling of human amnion from term (with and without labor) and preterm deliveries. Using the Illumina Infinium HumanMethylation27 BeadChip, we identified genes exhibiting differential methylation associated with normal labor and preterm birth. Functional analysis of the differentially methylated genes revealed biologically relevant enriched gene sets. Bisulfite sequencing analysis of the promoter region of the oxytocin receptor (OXTR) gene detected two CpG dinucleotides showing significant methylation differences among the three groups of samples. Hypermethylation of the CpG island of the solute carrier family 30 member 3 (SLC30A3) gene in preterm amnion was confirmed by methylation-specific PCR. This work provides preliminary evidence that DNA methylation changes in the amnion may be at least partially involved in the physiological process of labor and the etiology of preterm birth and suggests that DNA methylation profiles, in combination with other biological data, may provide valuable insight into the mechanisms underlying normal and pathological pregnancies. © 2013 Jinsil Kim et al.Fil: Kim, Jinsil. University of Iowa; Estados UnidosFil: Pitlick, Mitchell M.. University of Iowa; Estados UnidosFil: Christine, Paul J.. University of Iowa; Estados UnidosFil: Schaefer, Amanda R.. University of Iowa; Estados UnidosFil: Saleme, Cesar. Instituto de Maternidad y Ginecología Nuestra Señora de las Mercedes; ArgentinaFil: Comas, Belén. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina. Centro de Educación Médica e Investigaciones Clínicas “Norberto Quirno”; ArgentinaFil: Cosentino, Viviana Raquel. Centro de Educación Médica e Investigaciones Clínicas “Norberto Quirno”; ArgentinaFil: Gadow, Enrique Curt. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina. Centro de Educación Médica e Investigaciones Clínicas “Norberto Quirno”; ArgentinaFil: Murray, Jeffrey C.. University of Iowa; Estados UnidosHINDAWI publishing Corporation2013-02info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionhttp://purl.org/coar/resource_type/c_6501info:ar-repo/semantics/articuloapplication/pdfapplication/pdfapplication/pdfhttp://hdl.handle.net/11336/77775Kim, Jinsil; Pitlick, Mitchell M.; Christine, Paul J.; Schaefer, Amanda R.; Saleme, Cesar; et al.; Genome-wide analysis of DNA methylation in human amnion; HINDAWI publishing Corporation; Scientific World Journal; 2013; 2-2013; 1-111537-744XCONICET DigitalCONICETenginfo:eu-repo/semantics/altIdentifier/doi/10.1155/2013/678156info:eu-repo/semantics/altIdentifier/url/https://www.hindawi.com/journals/tswj/2013/678156/info:eu-repo/semantics/openAccesshttps://creativecommons.org/licenses/by-nc-sa/2.5/ar/reponame:CONICET Digital (CONICET)instname:Consejo Nacional de Investigaciones Científicas y Técnicas2025-09-03T09:44:18Zoai:ri.conicet.gov.ar:11336/77775instacron:CONICETInstitucionalhttp://ri.conicet.gov.ar/Organismo científico-tecnológicoNo correspondehttp://ri.conicet.gov.ar/oai/requestdasensio@conicet.gov.ar; lcarlino@conicet.gov.arArgentinaNo correspondeNo correspondeNo correspondeopendoar:34982025-09-03 09:44:18.394CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicasfalse
dc.title.none.fl_str_mv Genome-wide analysis of DNA methylation in human amnion
title Genome-wide analysis of DNA methylation in human amnion
spellingShingle Genome-wide analysis of DNA methylation in human amnion
Kim, Jinsil
preterm birth
genome-wide DNA methylation
human placenta
title_short Genome-wide analysis of DNA methylation in human amnion
title_full Genome-wide analysis of DNA methylation in human amnion
title_fullStr Genome-wide analysis of DNA methylation in human amnion
title_full_unstemmed Genome-wide analysis of DNA methylation in human amnion
title_sort Genome-wide analysis of DNA methylation in human amnion
dc.creator.none.fl_str_mv Kim, Jinsil
Pitlick, Mitchell M.
Christine, Paul J.
Schaefer, Amanda R.
Saleme, Cesar
Comas, Belén
Cosentino, Viviana Raquel
Gadow, Enrique Curt
Murray, Jeffrey C.
author Kim, Jinsil
author_facet Kim, Jinsil
Pitlick, Mitchell M.
Christine, Paul J.
Schaefer, Amanda R.
Saleme, Cesar
Comas, Belén
Cosentino, Viviana Raquel
Gadow, Enrique Curt
Murray, Jeffrey C.
author_role author
author2 Pitlick, Mitchell M.
Christine, Paul J.
Schaefer, Amanda R.
Saleme, Cesar
Comas, Belén
Cosentino, Viviana Raquel
Gadow, Enrique Curt
Murray, Jeffrey C.
author2_role author
author
author
author
author
author
author
author
dc.subject.none.fl_str_mv preterm birth
genome-wide DNA methylation
human placenta
topic preterm birth
genome-wide DNA methylation
human placenta
purl_subject.fl_str_mv https://purl.org/becyt/ford/3.1
https://purl.org/becyt/ford/3
dc.description.none.fl_txt_mv The amnion is a specialized tissue in contact with the amniotic fluid, which is in a constantly changing state. To investigate the importance of epigenetic events in this tissue in the physiology and pathophysiology of pregnancy, we performed genome-wide DNA methylation profiling of human amnion from term (with and without labor) and preterm deliveries. Using the Illumina Infinium HumanMethylation27 BeadChip, we identified genes exhibiting differential methylation associated with normal labor and preterm birth. Functional analysis of the differentially methylated genes revealed biologically relevant enriched gene sets. Bisulfite sequencing analysis of the promoter region of the oxytocin receptor (OXTR) gene detected two CpG dinucleotides showing significant methylation differences among the three groups of samples. Hypermethylation of the CpG island of the solute carrier family 30 member 3 (SLC30A3) gene in preterm amnion was confirmed by methylation-specific PCR. This work provides preliminary evidence that DNA methylation changes in the amnion may be at least partially involved in the physiological process of labor and the etiology of preterm birth and suggests that DNA methylation profiles, in combination with other biological data, may provide valuable insight into the mechanisms underlying normal and pathological pregnancies. © 2013 Jinsil Kim et al.
Fil: Kim, Jinsil. University of Iowa; Estados Unidos
Fil: Pitlick, Mitchell M.. University of Iowa; Estados Unidos
Fil: Christine, Paul J.. University of Iowa; Estados Unidos
Fil: Schaefer, Amanda R.. University of Iowa; Estados Unidos
Fil: Saleme, Cesar. Instituto de Maternidad y Ginecología Nuestra Señora de las Mercedes; Argentina
Fil: Comas, Belén. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina. Centro de Educación Médica e Investigaciones Clínicas “Norberto Quirno”; Argentina
Fil: Cosentino, Viviana Raquel. Centro de Educación Médica e Investigaciones Clínicas “Norberto Quirno”; Argentina
Fil: Gadow, Enrique Curt. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina. Centro de Educación Médica e Investigaciones Clínicas “Norberto Quirno”; Argentina
Fil: Murray, Jeffrey C.. University of Iowa; Estados Unidos
description The amnion is a specialized tissue in contact with the amniotic fluid, which is in a constantly changing state. To investigate the importance of epigenetic events in this tissue in the physiology and pathophysiology of pregnancy, we performed genome-wide DNA methylation profiling of human amnion from term (with and without labor) and preterm deliveries. Using the Illumina Infinium HumanMethylation27 BeadChip, we identified genes exhibiting differential methylation associated with normal labor and preterm birth. Functional analysis of the differentially methylated genes revealed biologically relevant enriched gene sets. Bisulfite sequencing analysis of the promoter region of the oxytocin receptor (OXTR) gene detected two CpG dinucleotides showing significant methylation differences among the three groups of samples. Hypermethylation of the CpG island of the solute carrier family 30 member 3 (SLC30A3) gene in preterm amnion was confirmed by methylation-specific PCR. This work provides preliminary evidence that DNA methylation changes in the amnion may be at least partially involved in the physiological process of labor and the etiology of preterm birth and suggests that DNA methylation profiles, in combination with other biological data, may provide valuable insight into the mechanisms underlying normal and pathological pregnancies. © 2013 Jinsil Kim et al.
publishDate 2013
dc.date.none.fl_str_mv 2013-02
dc.type.none.fl_str_mv info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
http://purl.org/coar/resource_type/c_6501
info:ar-repo/semantics/articulo
format article
status_str publishedVersion
dc.identifier.none.fl_str_mv http://hdl.handle.net/11336/77775
Kim, Jinsil; Pitlick, Mitchell M.; Christine, Paul J.; Schaefer, Amanda R.; Saleme, Cesar; et al.; Genome-wide analysis of DNA methylation in human amnion; HINDAWI publishing Corporation; Scientific World Journal; 2013; 2-2013; 1-11
1537-744X
CONICET Digital
CONICET
url http://hdl.handle.net/11336/77775
identifier_str_mv Kim, Jinsil; Pitlick, Mitchell M.; Christine, Paul J.; Schaefer, Amanda R.; Saleme, Cesar; et al.; Genome-wide analysis of DNA methylation in human amnion; HINDAWI publishing Corporation; Scientific World Journal; 2013; 2-2013; 1-11
1537-744X
CONICET Digital
CONICET
dc.language.none.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv info:eu-repo/semantics/altIdentifier/doi/10.1155/2013/678156
info:eu-repo/semantics/altIdentifier/url/https://www.hindawi.com/journals/tswj/2013/678156/
dc.rights.none.fl_str_mv info:eu-repo/semantics/openAccess
https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
eu_rights_str_mv openAccess
rights_invalid_str_mv https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
dc.format.none.fl_str_mv application/pdf
application/pdf
application/pdf
dc.publisher.none.fl_str_mv HINDAWI publishing Corporation
publisher.none.fl_str_mv HINDAWI publishing Corporation
dc.source.none.fl_str_mv reponame:CONICET Digital (CONICET)
instname:Consejo Nacional de Investigaciones Científicas y Técnicas
reponame_str CONICET Digital (CONICET)
collection CONICET Digital (CONICET)
instname_str Consejo Nacional de Investigaciones Científicas y Técnicas
repository.name.fl_str_mv CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicas
repository.mail.fl_str_mv dasensio@conicet.gov.ar; lcarlino@conicet.gov.ar
_version_ 1842268656996712448
score 13.13397

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