A new method using acid to clean and a technique for preparation of eggs of biting midges (Diptera: Ceratopogonidae) for scanning electron microscope

Autores
Ronderos, Maria Marcela; Díaz, Florentina; Sarmiento, Patricia Laura
Año de publicación
2009
Idioma
inglés
Tipo de recurso
artículo
Estado
versión publicada
Descripción
A new procedure for clearing eggs belonging to genera of Ceratopogonidae is described. In the new procedure eggs in different states, with different ultra structures of the exochorion, were immersed in 10% glycolic acid (CH2OH-COOH) and 20 % mandellic acid (C6H5.CHOH.COOH) for 1-4 minutes. Eggs, prepared by both acids and subsequently neutralized by distilled water, were placed in a saturated solution of phenol, and then passed through a battery of alcohol solutions. Finally, critical point was performed and gold metallized. After preparation the eggs of this group retained their natural form under vacuum long enough to yield good microphotographs of the outer layer of the chorion.
Fil: Ronderos, Maria Marcela. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - La Plata. Centro de Estudios Parasitológicos y de Vectores. Universidad Nacional de La Plata. Facultad de Ciencias Naturales y Museo. Centro de Estudios Parasitológicos y de Vectores; Argentina. Universidad Nacional de La Plata. Facultad de Ciencias Naturales y Museo. Departamento Científico de Entomología; Argentina
Fil: Díaz, Florentina. Museo de La Plata; Argentina. Universidad Nacional de La Plata. Facultad de Ciencias Naturales y Museo. Departamento Científico de Entomología; Argentina
Fil: Sarmiento, Patricia Laura. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - La Plata; Argentina. Universidad Nacional de La Plata. Facultad de Ciencias Naturales y Museo. Departamento Científico de Entomología; Argentina
Materia
Technique
SEM
EGGS
Ceratopogonidae
Nivel de accesibilidad
acceso abierto
Condiciones de uso
https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
Repositorio
CONICET Digital (CONICET)
Institución
Consejo Nacional de Investigaciones Científicas y Técnicas
OAI Identificador
oai:ri.conicet.gov.ar:11336/129543
Acceder
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oai_identifier_str oai:ri.conicet.gov.ar:11336/129543
network_acronym_str CONICETDig
repository_id_str 3498
network_name_str CONICET Digital (CONICET)
spelling A new method using acid to clean and a technique for preparation of eggs of biting midges (Diptera: Ceratopogonidae) for scanning electron microscopeRonderos, Maria MarcelaDíaz, FlorentinaSarmiento, Patricia LauraTechniqueSEMEGGSCeratopogonidaehttps://purl.org/becyt/ford/1.6https://purl.org/becyt/ford/1A new procedure for clearing eggs belonging to genera of Ceratopogonidae is described. In the new procedure eggs in different states, with different ultra structures of the exochorion, were immersed in 10% glycolic acid (CH2OH-COOH) and 20 % mandellic acid (C6H5.CHOH.COOH) for 1-4 minutes. Eggs, prepared by both acids and subsequently neutralized by distilled water, were placed in a saturated solution of phenol, and then passed through a battery of alcohol solutions. Finally, critical point was performed and gold metallized. After preparation the eggs of this group retained their natural form under vacuum long enough to yield good microphotographs of the outer layer of the chorion.Fil: Ronderos, Maria Marcela. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - La Plata. Centro de Estudios Parasitológicos y de Vectores. Universidad Nacional de La Plata. Facultad de Ciencias Naturales y Museo. Centro de Estudios Parasitológicos y de Vectores; Argentina. Universidad Nacional de La Plata. Facultad de Ciencias Naturales y Museo. Departamento Científico de Entomología; ArgentinaFil: Díaz, Florentina. Museo de La Plata; Argentina. Universidad Nacional de La Plata. Facultad de Ciencias Naturales y Museo. Departamento Científico de Entomología; ArgentinaFil: Sarmiento, Patricia Laura. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - La Plata; Argentina. Universidad Nacional de La Plata. Facultad de Ciencias Naturales y Museo. Departamento Científico de Entomología; ArgentinaAmerican Entomological Society2009-12info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionhttp://purl.org/coar/resource_type/c_6501info:ar-repo/semantics/articuloapplication/pdfapplication/pdfhttp://hdl.handle.net/11336/129543Ronderos, Maria Marcela; Díaz, Florentina; Sarmiento, Patricia Laura; A new method using acid to clean and a technique for preparation of eggs of biting midges (Diptera: Ceratopogonidae) for scanning electron microscope; American Entomological Society; Transactions of the American Entomological Society; 134; 3; 12-2009; 471-4760002-8320CONICET DigitalCONICETenginfo:eu-repo/semantics/altIdentifier/doi/10.3157/0002-8320-134.3.471info:eu-repo/semantics/altIdentifier/url/https://bioone.org/journals/transactions-of-the-american-entomological-society/volume-134/issue-3/0002-8320-134.3.471/A-new-method-using-acid-to-clean-and-a-technique/10.3157/0002-8320-134.3.471.shortinfo:eu-repo/semantics/openAccesshttps://creativecommons.org/licenses/by-nc-sa/2.5/ar/reponame:CONICET Digital (CONICET)instname:Consejo Nacional de Investigaciones Científicas y Técnicas2025-09-29T10:03:11Zoai:ri.conicet.gov.ar:11336/129543instacron:CONICETInstitucionalhttp://ri.conicet.gov.ar/Organismo científico-tecnológicoNo correspondehttp://ri.conicet.gov.ar/oai/requestdasensio@conicet.gov.ar; lcarlino@conicet.gov.arArgentinaNo correspondeNo correspondeNo correspondeopendoar:34982025-09-29 10:03:12.186CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicasfalse
dc.title.none.fl_str_mv A new method using acid to clean and a technique for preparation of eggs of biting midges (Diptera: Ceratopogonidae) for scanning electron microscope
title A new method using acid to clean and a technique for preparation of eggs of biting midges (Diptera: Ceratopogonidae) for scanning electron microscope
spellingShingle A new method using acid to clean and a technique for preparation of eggs of biting midges (Diptera: Ceratopogonidae) for scanning electron microscope
Ronderos, Maria Marcela
Technique
SEM
EGGS
Ceratopogonidae
title_short A new method using acid to clean and a technique for preparation of eggs of biting midges (Diptera: Ceratopogonidae) for scanning electron microscope
title_full A new method using acid to clean and a technique for preparation of eggs of biting midges (Diptera: Ceratopogonidae) for scanning electron microscope
title_fullStr A new method using acid to clean and a technique for preparation of eggs of biting midges (Diptera: Ceratopogonidae) for scanning electron microscope
title_full_unstemmed A new method using acid to clean and a technique for preparation of eggs of biting midges (Diptera: Ceratopogonidae) for scanning electron microscope
title_sort A new method using acid to clean and a technique for preparation of eggs of biting midges (Diptera: Ceratopogonidae) for scanning electron microscope
dc.creator.none.fl_str_mv Ronderos, Maria Marcela
Díaz, Florentina
Sarmiento, Patricia Laura
author Ronderos, Maria Marcela
author_facet Ronderos, Maria Marcela
Díaz, Florentina
Sarmiento, Patricia Laura
author_role author
author2 Díaz, Florentina
Sarmiento, Patricia Laura
author2_role author
author
dc.subject.none.fl_str_mv Technique
SEM
EGGS
Ceratopogonidae
topic Technique
SEM
EGGS
Ceratopogonidae
purl_subject.fl_str_mv https://purl.org/becyt/ford/1.6
https://purl.org/becyt/ford/1
dc.description.none.fl_txt_mv A new procedure for clearing eggs belonging to genera of Ceratopogonidae is described. In the new procedure eggs in different states, with different ultra structures of the exochorion, were immersed in 10% glycolic acid (CH2OH-COOH) and 20 % mandellic acid (C6H5.CHOH.COOH) for 1-4 minutes. Eggs, prepared by both acids and subsequently neutralized by distilled water, were placed in a saturated solution of phenol, and then passed through a battery of alcohol solutions. Finally, critical point was performed and gold metallized. After preparation the eggs of this group retained their natural form under vacuum long enough to yield good microphotographs of the outer layer of the chorion.
Fil: Ronderos, Maria Marcela. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - La Plata. Centro de Estudios Parasitológicos y de Vectores. Universidad Nacional de La Plata. Facultad de Ciencias Naturales y Museo. Centro de Estudios Parasitológicos y de Vectores; Argentina. Universidad Nacional de La Plata. Facultad de Ciencias Naturales y Museo. Departamento Científico de Entomología; Argentina
Fil: Díaz, Florentina. Museo de La Plata; Argentina. Universidad Nacional de La Plata. Facultad de Ciencias Naturales y Museo. Departamento Científico de Entomología; Argentina
Fil: Sarmiento, Patricia Laura. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - La Plata; Argentina. Universidad Nacional de La Plata. Facultad de Ciencias Naturales y Museo. Departamento Científico de Entomología; Argentina
description A new procedure for clearing eggs belonging to genera of Ceratopogonidae is described. In the new procedure eggs in different states, with different ultra structures of the exochorion, were immersed in 10% glycolic acid (CH2OH-COOH) and 20 % mandellic acid (C6H5.CHOH.COOH) for 1-4 minutes. Eggs, prepared by both acids and subsequently neutralized by distilled water, were placed in a saturated solution of phenol, and then passed through a battery of alcohol solutions. Finally, critical point was performed and gold metallized. After preparation the eggs of this group retained their natural form under vacuum long enough to yield good microphotographs of the outer layer of the chorion.
publishDate 2009
dc.date.none.fl_str_mv 2009-12
dc.type.none.fl_str_mv info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
http://purl.org/coar/resource_type/c_6501
info:ar-repo/semantics/articulo
format article
status_str publishedVersion
dc.identifier.none.fl_str_mv http://hdl.handle.net/11336/129543
Ronderos, Maria Marcela; Díaz, Florentina; Sarmiento, Patricia Laura; A new method using acid to clean and a technique for preparation of eggs of biting midges (Diptera: Ceratopogonidae) for scanning electron microscope; American Entomological Society; Transactions of the American Entomological Society; 134; 3; 12-2009; 471-476
0002-8320
CONICET Digital
CONICET
url http://hdl.handle.net/11336/129543
identifier_str_mv Ronderos, Maria Marcela; Díaz, Florentina; Sarmiento, Patricia Laura; A new method using acid to clean and a technique for preparation of eggs of biting midges (Diptera: Ceratopogonidae) for scanning electron microscope; American Entomological Society; Transactions of the American Entomological Society; 134; 3; 12-2009; 471-476
0002-8320
CONICET Digital
CONICET
dc.language.none.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv info:eu-repo/semantics/altIdentifier/doi/10.3157/0002-8320-134.3.471
info:eu-repo/semantics/altIdentifier/url/https://bioone.org/journals/transactions-of-the-american-entomological-society/volume-134/issue-3/0002-8320-134.3.471/A-new-method-using-acid-to-clean-and-a-technique/10.3157/0002-8320-134.3.471.short
dc.rights.none.fl_str_mv info:eu-repo/semantics/openAccess
https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
eu_rights_str_mv openAccess
rights_invalid_str_mv https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
dc.format.none.fl_str_mv application/pdf
application/pdf
dc.publisher.none.fl_str_mv American Entomological Society
publisher.none.fl_str_mv American Entomological Society
dc.source.none.fl_str_mv reponame:CONICET Digital (CONICET)
instname:Consejo Nacional de Investigaciones Científicas y Técnicas
reponame_str CONICET Digital (CONICET)
collection CONICET Digital (CONICET)
instname_str Consejo Nacional de Investigaciones Científicas y Técnicas
repository.name.fl_str_mv CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicas
repository.mail.fl_str_mv dasensio@conicet.gov.ar; lcarlino@conicet.gov.ar
_version_ 1844613845251784704
score 13.070432

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