Increased phosphate solubilization by rhizospheric sugarcane interspecies interactions

Autores
Rubio Molina, Anna Carolina; Bulacio Gil, Natalia María; Zenoff, Ana Maria; Caram Di Santo, Maria Carolina; de Cristobal, Ricardo Ezequiel; Pomares, Maria Fernanda; Vincent, Paula Andrea; Adler, Conrado
Año de publicación
2019
Idioma
inglés
Tipo de recurso
documento de conferencia
Estado
versión publicada
Descripción
Plant-associated microbiota can enormously influence on plant traits. Manipulation of these microbial communities holds great potential as an effective way to improve crops, while contributing to a more environmentally benign agriculture. Revealing the chemical communication network in the rhizosphere will pave the way for the future production of bioinoculants using bacterial consortia. In this work, we isolated numerous microorganisms from the sugarcane rhizosphere, sequenced the 16S rDNA of a subset of isolates showing different cultural features and evaluated their ability to solubilize phosphate in NBRIP medium. Next, using only phylogenetically different isolates growing in solid medium containing salts, amino acids and sucrose, we tested interspecies interactions in pairwise combinations. Interactions were followed for 7 days and cultural traits such as growth inhibition, growth promotion, colony morphology changes and pigment production were registered. We focused in one peculiar interaction that involved isolate 258 promoting growth of isolate 214 while the latter inhibited growth of 258. In addition, 258 enhanced the ability of 214 to solubilize phosphate. To analyze if this phenomenon was due to a secreted metabolite, supernatants were tested. Interestingly, we observed that cell-free supernatants of isolate 258 were able to induce 214 phosphate solubilization. We are currently performing experiments to elucidate the chemical nature of the metabolites responsible for this phenotype.
Fil: Rubio Molina, Anna Carolina. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Tucumán. Instituto Superior de Investigaciones Biológicas. Universidad Nacional de Tucumán. Instituto Superior de Investigaciones Biológicas; Argentina
Fil: Bulacio Gil, Natalia María. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Tucumán. Instituto Superior de Investigaciones Biológicas. Universidad Nacional de Tucumán. Instituto Superior de Investigaciones Biológicas; Argentina
Fil: Zenoff, Ana Maria. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Tucumán. Instituto Superior de Investigaciones Biológicas. Universidad Nacional de Tucumán. Instituto Superior de Investigaciones Biológicas; Argentina
Fil: Caram Di Santo, Maria Carolina. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Tucumán. Instituto Superior de Investigaciones Biológicas. Universidad Nacional de Tucumán. Instituto Superior de Investigaciones Biológicas; Argentina
Fil: de Cristobal, Ricardo Ezequiel. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Tucumán. Instituto Superior de Investigaciones Biológicas. Universidad Nacional de Tucumán. Instituto Superior de Investigaciones Biológicas; Argentina
Fil: Pomares, Maria Fernanda. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Tucumán. Instituto Superior de Investigaciones Biológicas. Universidad Nacional de Tucumán. Instituto Superior de Investigaciones Biológicas; Argentina
Fil: Vincent, Paula Andrea. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Tucumán. Instituto Superior de Investigaciones Biológicas. Universidad Nacional de Tucumán. Instituto Superior de Investigaciones Biológicas; Argentina
Fil: Adler, Conrado. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Tucumán. Instituto Superior de Investigaciones Biológicas. Universidad Nacional de Tucumán. Instituto Superior de Investigaciones Biológicas; Argentina
The LV Annual SAIB Meeting and XIV PABMB Congress
Salta
Argentina
Sociedad Argentina de Investigaciones Bioquímicas
Materia
PHOSPHATE SOLUBILIZATION
INTERSPECIES INTERACTIONS
sugarcane
Nivel de accesibilidad
acceso abierto
Condiciones de uso
https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
Repositorio
CONICET Digital (CONICET)
Institución
Consejo Nacional de Investigaciones Científicas y Técnicas
OAI Identificador
oai:ri.conicet.gov.ar:11336/181793
Acceder
id CONICETDig_13807bb46e44de193e193e67db60beaa
oai_identifier_str oai:ri.conicet.gov.ar:11336/181793
network_acronym_str CONICETDig
repository_id_str 3498
network_name_str CONICET Digital (CONICET)
spelling Increased phosphate solubilization by rhizospheric sugarcane interspecies interactionsRubio Molina, Anna CarolinaBulacio Gil, Natalia MaríaZenoff, Ana MariaCaram Di Santo, Maria Carolinade Cristobal, Ricardo EzequielPomares, Maria FernandaVincent, Paula AndreaAdler, ConradoPHOSPHATE SOLUBILIZATIONINTERSPECIES INTERACTIONSsugarcanehttps://purl.org/becyt/ford/1.6https://purl.org/becyt/ford/1Plant-associated microbiota can enormously influence on plant traits. Manipulation of these microbial communities holds great potential as an effective way to improve crops, while contributing to a more environmentally benign agriculture. Revealing the chemical communication network in the rhizosphere will pave the way for the future production of bioinoculants using bacterial consortia. In this work, we isolated numerous microorganisms from the sugarcane rhizosphere, sequenced the 16S rDNA of a subset of isolates showing different cultural features and evaluated their ability to solubilize phosphate in NBRIP medium. Next, using only phylogenetically different isolates growing in solid medium containing salts, amino acids and sucrose, we tested interspecies interactions in pairwise combinations. Interactions were followed for 7 days and cultural traits such as growth inhibition, growth promotion, colony morphology changes and pigment production were registered. We focused in one peculiar interaction that involved isolate 258 promoting growth of isolate 214 while the latter inhibited growth of 258. In addition, 258 enhanced the ability of 214 to solubilize phosphate. To analyze if this phenomenon was due to a secreted metabolite, supernatants were tested. Interestingly, we observed that cell-free supernatants of isolate 258 were able to induce 214 phosphate solubilization. We are currently performing experiments to elucidate the chemical nature of the metabolites responsible for this phenotype.Fil: Rubio Molina, Anna Carolina. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Tucumán. Instituto Superior de Investigaciones Biológicas. Universidad Nacional de Tucumán. Instituto Superior de Investigaciones Biológicas; ArgentinaFil: Bulacio Gil, Natalia María. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Tucumán. Instituto Superior de Investigaciones Biológicas. Universidad Nacional de Tucumán. Instituto Superior de Investigaciones Biológicas; ArgentinaFil: Zenoff, Ana Maria. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Tucumán. Instituto Superior de Investigaciones Biológicas. Universidad Nacional de Tucumán. Instituto Superior de Investigaciones Biológicas; ArgentinaFil: Caram Di Santo, Maria Carolina. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Tucumán. Instituto Superior de Investigaciones Biológicas. Universidad Nacional de Tucumán. Instituto Superior de Investigaciones Biológicas; ArgentinaFil: de Cristobal, Ricardo Ezequiel. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Tucumán. Instituto Superior de Investigaciones Biológicas. Universidad Nacional de Tucumán. Instituto Superior de Investigaciones Biológicas; ArgentinaFil: Pomares, Maria Fernanda. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Tucumán. Instituto Superior de Investigaciones Biológicas. Universidad Nacional de Tucumán. Instituto Superior de Investigaciones Biológicas; ArgentinaFil: Vincent, Paula Andrea. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Tucumán. Instituto Superior de Investigaciones Biológicas. Universidad Nacional de Tucumán. Instituto Superior de Investigaciones Biológicas; ArgentinaFil: Adler, Conrado. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Tucumán. Instituto Superior de Investigaciones Biológicas. Universidad Nacional de Tucumán. Instituto Superior de Investigaciones Biológicas; ArgentinaThe LV Annual SAIB Meeting and XIV PABMB CongressSaltaArgentinaSociedad Argentina de Investigaciones BioquímicasTech Science Press2019info:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/conferenceObjectReuniónJournalhttp://purl.org/coar/resource_type/c_5794info:ar-repo/semantics/documentoDeConferenciaapplication/pdfapplication/pdfapplication/pdfapplication/pdfapplication/pdfapplication/pdfapplication/pdfapplication/pdfhttp://hdl.handle.net/11336/181793Increased phosphate solubilization by rhizospheric sugarcane interspecies interactions; The LV Annual SAIB Meeting and XIV PABMB Congress; Salta; Argentina; 2019; 1-6CONICET DigitalCONICETenginfo:eu-repo/semantics/altIdentifier/url/https://techscience.com/biocell/v43nSuppl.5/33868/pdfInternacionalinfo:eu-repo/semantics/openAccesshttps://creativecommons.org/licenses/by-nc-sa/2.5/ar/reponame:CONICET Digital (CONICET)instname:Consejo Nacional de Investigaciones Científicas y Técnicas2025-10-15T14:40:56Zoai:ri.conicet.gov.ar:11336/181793instacron:CONICETInstitucionalhttp://ri.conicet.gov.ar/Organismo científico-tecnológicoNo correspondehttp://ri.conicet.gov.ar/oai/requestdasensio@conicet.gov.ar; lcarlino@conicet.gov.arArgentinaNo correspondeNo correspondeNo correspondeopendoar:34982025-10-15 14:40:57.004CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicasfalse
dc.title.none.fl_str_mv Increased phosphate solubilization by rhizospheric sugarcane interspecies interactions
title Increased phosphate solubilization by rhizospheric sugarcane interspecies interactions
spellingShingle Increased phosphate solubilization by rhizospheric sugarcane interspecies interactions
Rubio Molina, Anna Carolina
PHOSPHATE SOLUBILIZATION
INTERSPECIES INTERACTIONS
sugarcane
title_short Increased phosphate solubilization by rhizospheric sugarcane interspecies interactions
title_full Increased phosphate solubilization by rhizospheric sugarcane interspecies interactions
title_fullStr Increased phosphate solubilization by rhizospheric sugarcane interspecies interactions
title_full_unstemmed Increased phosphate solubilization by rhizospheric sugarcane interspecies interactions
title_sort Increased phosphate solubilization by rhizospheric sugarcane interspecies interactions
dc.creator.none.fl_str_mv Rubio Molina, Anna Carolina
Bulacio Gil, Natalia María
Zenoff, Ana Maria
Caram Di Santo, Maria Carolina
de Cristobal, Ricardo Ezequiel
Pomares, Maria Fernanda
Vincent, Paula Andrea
Adler, Conrado
author Rubio Molina, Anna Carolina
author_facet Rubio Molina, Anna Carolina
Bulacio Gil, Natalia María
Zenoff, Ana Maria
Caram Di Santo, Maria Carolina
de Cristobal, Ricardo Ezequiel
Pomares, Maria Fernanda
Vincent, Paula Andrea
Adler, Conrado
author_role author
author2 Bulacio Gil, Natalia María
Zenoff, Ana Maria
Caram Di Santo, Maria Carolina
de Cristobal, Ricardo Ezequiel
Pomares, Maria Fernanda
Vincent, Paula Andrea
Adler, Conrado
author2_role author
author
author
author
author
author
author
dc.subject.none.fl_str_mv PHOSPHATE SOLUBILIZATION
INTERSPECIES INTERACTIONS
sugarcane
topic PHOSPHATE SOLUBILIZATION
INTERSPECIES INTERACTIONS
sugarcane
purl_subject.fl_str_mv https://purl.org/becyt/ford/1.6
https://purl.org/becyt/ford/1
dc.description.none.fl_txt_mv Plant-associated microbiota can enormously influence on plant traits. Manipulation of these microbial communities holds great potential as an effective way to improve crops, while contributing to a more environmentally benign agriculture. Revealing the chemical communication network in the rhizosphere will pave the way for the future production of bioinoculants using bacterial consortia. In this work, we isolated numerous microorganisms from the sugarcane rhizosphere, sequenced the 16S rDNA of a subset of isolates showing different cultural features and evaluated their ability to solubilize phosphate in NBRIP medium. Next, using only phylogenetically different isolates growing in solid medium containing salts, amino acids and sucrose, we tested interspecies interactions in pairwise combinations. Interactions were followed for 7 days and cultural traits such as growth inhibition, growth promotion, colony morphology changes and pigment production were registered. We focused in one peculiar interaction that involved isolate 258 promoting growth of isolate 214 while the latter inhibited growth of 258. In addition, 258 enhanced the ability of 214 to solubilize phosphate. To analyze if this phenomenon was due to a secreted metabolite, supernatants were tested. Interestingly, we observed that cell-free supernatants of isolate 258 were able to induce 214 phosphate solubilization. We are currently performing experiments to elucidate the chemical nature of the metabolites responsible for this phenotype.
Fil: Rubio Molina, Anna Carolina. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Tucumán. Instituto Superior de Investigaciones Biológicas. Universidad Nacional de Tucumán. Instituto Superior de Investigaciones Biológicas; Argentina
Fil: Bulacio Gil, Natalia María. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Tucumán. Instituto Superior de Investigaciones Biológicas. Universidad Nacional de Tucumán. Instituto Superior de Investigaciones Biológicas; Argentina
Fil: Zenoff, Ana Maria. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Tucumán. Instituto Superior de Investigaciones Biológicas. Universidad Nacional de Tucumán. Instituto Superior de Investigaciones Biológicas; Argentina
Fil: Caram Di Santo, Maria Carolina. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Tucumán. Instituto Superior de Investigaciones Biológicas. Universidad Nacional de Tucumán. Instituto Superior de Investigaciones Biológicas; Argentina
Fil: de Cristobal, Ricardo Ezequiel. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Tucumán. Instituto Superior de Investigaciones Biológicas. Universidad Nacional de Tucumán. Instituto Superior de Investigaciones Biológicas; Argentina
Fil: Pomares, Maria Fernanda. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Tucumán. Instituto Superior de Investigaciones Biológicas. Universidad Nacional de Tucumán. Instituto Superior de Investigaciones Biológicas; Argentina
Fil: Vincent, Paula Andrea. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Tucumán. Instituto Superior de Investigaciones Biológicas. Universidad Nacional de Tucumán. Instituto Superior de Investigaciones Biológicas; Argentina
Fil: Adler, Conrado. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Tucumán. Instituto Superior de Investigaciones Biológicas. Universidad Nacional de Tucumán. Instituto Superior de Investigaciones Biológicas; Argentina
The LV Annual SAIB Meeting and XIV PABMB Congress
Salta
Argentina
Sociedad Argentina de Investigaciones Bioquímicas
description Plant-associated microbiota can enormously influence on plant traits. Manipulation of these microbial communities holds great potential as an effective way to improve crops, while contributing to a more environmentally benign agriculture. Revealing the chemical communication network in the rhizosphere will pave the way for the future production of bioinoculants using bacterial consortia. In this work, we isolated numerous microorganisms from the sugarcane rhizosphere, sequenced the 16S rDNA of a subset of isolates showing different cultural features and evaluated their ability to solubilize phosphate in NBRIP medium. Next, using only phylogenetically different isolates growing in solid medium containing salts, amino acids and sucrose, we tested interspecies interactions in pairwise combinations. Interactions were followed for 7 days and cultural traits such as growth inhibition, growth promotion, colony morphology changes and pigment production were registered. We focused in one peculiar interaction that involved isolate 258 promoting growth of isolate 214 while the latter inhibited growth of 258. In addition, 258 enhanced the ability of 214 to solubilize phosphate. To analyze if this phenomenon was due to a secreted metabolite, supernatants were tested. Interestingly, we observed that cell-free supernatants of isolate 258 were able to induce 214 phosphate solubilization. We are currently performing experiments to elucidate the chemical nature of the metabolites responsible for this phenotype.
publishDate 2019
dc.date.none.fl_str_mv 2019
dc.type.none.fl_str_mv info:eu-repo/semantics/publishedVersion
info:eu-repo/semantics/conferenceObject
Reunión
Journal
http://purl.org/coar/resource_type/c_5794
info:ar-repo/semantics/documentoDeConferencia
status_str publishedVersion
format conferenceObject
dc.identifier.none.fl_str_mv http://hdl.handle.net/11336/181793
Increased phosphate solubilization by rhizospheric sugarcane interspecies interactions; The LV Annual SAIB Meeting and XIV PABMB Congress; Salta; Argentina; 2019; 1-6
CONICET Digital
CONICET
url http://hdl.handle.net/11336/181793
identifier_str_mv Increased phosphate solubilization by rhizospheric sugarcane interspecies interactions; The LV Annual SAIB Meeting and XIV PABMB Congress; Salta; Argentina; 2019; 1-6
CONICET Digital
CONICET
dc.language.none.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv info:eu-repo/semantics/altIdentifier/url/https://techscience.com/biocell/v43nSuppl.5/33868/pdf
dc.rights.none.fl_str_mv info:eu-repo/semantics/openAccess
https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
eu_rights_str_mv openAccess
rights_invalid_str_mv https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
dc.format.none.fl_str_mv application/pdf
application/pdf
application/pdf
application/pdf
application/pdf
application/pdf
application/pdf
application/pdf
dc.coverage.none.fl_str_mv Internacional
dc.publisher.none.fl_str_mv Tech Science Press
publisher.none.fl_str_mv Tech Science Press
dc.source.none.fl_str_mv reponame:CONICET Digital (CONICET)
instname:Consejo Nacional de Investigaciones Científicas y Técnicas
reponame_str CONICET Digital (CONICET)
collection CONICET Digital (CONICET)
instname_str Consejo Nacional de Investigaciones Científicas y Técnicas
repository.name.fl_str_mv CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicas
repository.mail.fl_str_mv dasensio@conicet.gov.ar; lcarlino@conicet.gov.ar
_version_ 1846082903814963200
score 13.22299

Publicaciones similares