Metagenomic approach of associated fungi with megaplatypus mutatus (Coleoptera: Platypodinae)

Autores
Ceriani Nakamurakare, Esteban Daniel; Ramos, Sergio; Robles, Carolina Analía; Novas, María Victoria; D'jonsiles, María Fernanda; Gonzalez Audino, Paola Andrea; Carmaran, Cecilia Cristina
Año de publicación
2018
Idioma
inglés
Tipo de recurso
artículo
Estado
versión publicada
Descripción
Megaplatypus mutatus is a major forestpest in Argentina and an emerging pest in Europe. In this study the multitrophic interactions between M. mutatus and associated fungi were assessed with ametagenomics approach (454-pyrosequencing). A total of 270 collection pointsfrom insect galleries from three locations in Argentina were pooled for pyrosequencing analyses. Two hosts, Populus deltoides and Casuarina cunninghamiana, were independently evaluated to characterize the fungal communities associated to M.mutatus; compare the culture-independent approach with previous culturingstudies, in terms of data recovery related to the fungal community composition,and test the specificity of the fungal communities amongst locations and hosts. A Generalized Linear Mixed Model was performed to compare the fungal richnessin each dataset, which showed no significant differences between taxa richness amongst locations. Principal Coordinates Analysesshowed a separation between fungal communities within the same host, suggesting that host identity would not be crucial to determine the specificity in fungal communities. Candida insectalens and one Fusarium species, present in all hosts and locations, achieved 37.6 % of the total relative frequency per taxa. These results complement the data from culturing methods previously reported, thus improving the accuracy and understanding of the fungal assemblages associated to M. mutatus.
Fil: Ceriani Nakamurakare, Esteban Daniel. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales. Departamento de Biodiversidad y Biología Experimental; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina
Fil: Ramos, Sergio. Instituto Nacional de Tecnologia Agropecuaria. Centro Regional Entre Rios. Estacion Experimental Agropecuaria Concordia.; Argentina
Fil: Robles, Carolina Analía. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales. Departamento de Biodiversidad y Biología Experimental; Argentina
Fil: Novas, María Victoria. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Ciudad Universitaria. Instituto de Micología y Botánica. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales. Instituto de Micología y Botánica; Argentina
Fil: D'jonsiles, María Fernanda. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales. Departamento de Biodiversidad y Biología Experimental; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina
Fil: Gonzalez Audino, Paola Andrea. Consejo Nacional de Investigaciones Científicas y Técnicas. Instituto de Investigaciones Científicas y Técnicas para la Defensa. Centro de Investigación de Plagas e Insecticidas; Argentina
Fil: Carmaran, Cecilia Cristina. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales. Departamento de Biodiversidad y Biología Experimental; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina
Materia
AMBROSIA
FOREST PEST
METAGENOMIC
FUNGI
PLATYPODINAE
INSECT-FUNGUS INTERACTION
Nivel de accesibilidad
acceso abierto
Condiciones de uso
https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
Repositorio
CONICET Digital (CONICET)
Institución
Consejo Nacional de Investigaciones Científicas y Técnicas
OAI Identificador
oai:ri.conicet.gov.ar:11336/160559
Acceder
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oai_identifier_str oai:ri.conicet.gov.ar:11336/160559
network_acronym_str CONICETDig
repository_id_str 3498
network_name_str CONICET Digital (CONICET)
spelling Metagenomic approach of associated fungi with megaplatypus mutatus (Coleoptera: Platypodinae)Ceriani Nakamurakare, Esteban DanielRamos, SergioRobles, Carolina AnalíaNovas, María VictoriaD'jonsiles, María FernandaGonzalez Audino, Paola AndreaCarmaran, Cecilia CristinaAMBROSIAFOREST PESTMETAGENOMICFUNGIPLATYPODINAEINSECT-FUNGUS INTERACTIONhttps://purl.org/becyt/ford/1.6https://purl.org/becyt/ford/1https://purl.org/becyt/ford/1.6https://purl.org/becyt/ford/1Megaplatypus mutatus is a major forestpest in Argentina and an emerging pest in Europe. In this study the multitrophic interactions between M. mutatus and associated fungi were assessed with ametagenomics approach (454-pyrosequencing). A total of 270 collection pointsfrom insect galleries from three locations in Argentina were pooled for pyrosequencing analyses. Two hosts, Populus deltoides and Casuarina cunninghamiana, were independently evaluated to characterize the fungal communities associated to M.mutatus; compare the culture-independent approach with previous culturingstudies, in terms of data recovery related to the fungal community composition,and test the specificity of the fungal communities amongst locations and hosts. A Generalized Linear Mixed Model was performed to compare the fungal richnessin each dataset, which showed no significant differences between taxa richness amongst locations. Principal Coordinates Analysesshowed a separation between fungal communities within the same host, suggesting that host identity would not be crucial to determine the specificity in fungal communities. Candida insectalens and one Fusarium species, present in all hosts and locations, achieved 37.6 % of the total relative frequency per taxa. These results complement the data from culturing methods previously reported, thus improving the accuracy and understanding of the fungal assemblages associated to M. mutatus.Fil: Ceriani Nakamurakare, Esteban Daniel. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales. Departamento de Biodiversidad y Biología Experimental; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; ArgentinaFil: Ramos, Sergio. Instituto Nacional de Tecnologia Agropecuaria. Centro Regional Entre Rios. Estacion Experimental Agropecuaria Concordia.; ArgentinaFil: Robles, Carolina Analía. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales. Departamento de Biodiversidad y Biología Experimental; ArgentinaFil: Novas, María Victoria. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Ciudad Universitaria. Instituto de Micología y Botánica. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales. Instituto de Micología y Botánica; ArgentinaFil: D'jonsiles, María Fernanda. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales. Departamento de Biodiversidad y Biología Experimental; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; ArgentinaFil: Gonzalez Audino, Paola Andrea. Consejo Nacional de Investigaciones Científicas y Técnicas. Instituto de Investigaciones Científicas y Técnicas para la Defensa. Centro de Investigación de Plagas e Insecticidas; ArgentinaFil: Carmaran, Cecilia Cristina. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales. Departamento de Biodiversidad y Biología Experimental; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; ArgentinaFinnish Soc Forest Sciencefinnish Forest Research2018-05info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionhttp://purl.org/coar/resource_type/c_6501info:ar-repo/semantics/articuloapplication/pdfapplication/pdfapplication/pdfhttp://hdl.handle.net/11336/160559Ceriani Nakamurakare, Esteban Daniel; Ramos, Sergio; Robles, Carolina Analía; Novas, María Victoria; D'jonsiles, María Fernanda; et al.; Metagenomic approach of associated fungi with megaplatypus mutatus (Coleoptera: Platypodinae); Finnish Soc Forest Sciencefinnish Forest Research; Silva Fennica; 52; 3; 5-2018; 1-160037-5330CONICET DigitalCONICETenginfo:eu-repo/semantics/altIdentifier/url/https://www.silvafennica.fi/pdf/article9940.pdfinfo:eu-repo/semantics/altIdentifier/doi/10.14214/sf.9940info:eu-repo/semantics/altIdentifier/url/https://www.silvafennica.fi/article/9940info:eu-repo/semantics/openAccesshttps://creativecommons.org/licenses/by-nc-sa/2.5/ar/reponame:CONICET Digital (CONICET)instname:Consejo Nacional de Investigaciones Científicas y Técnicas2025-10-22T11:53:55Zoai:ri.conicet.gov.ar:11336/160559instacron:CONICETInstitucionalhttp://ri.conicet.gov.ar/Organismo científico-tecnológicoNo correspondehttp://ri.conicet.gov.ar/oai/requestdasensio@conicet.gov.ar; lcarlino@conicet.gov.arArgentinaNo correspondeNo correspondeNo correspondeopendoar:34982025-10-22 11:53:55.932CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicasfalse
dc.title.none.fl_str_mv Metagenomic approach of associated fungi with megaplatypus mutatus (Coleoptera: Platypodinae)
title Metagenomic approach of associated fungi with megaplatypus mutatus (Coleoptera: Platypodinae)
spellingShingle Metagenomic approach of associated fungi with megaplatypus mutatus (Coleoptera: Platypodinae)
Ceriani Nakamurakare, Esteban Daniel
AMBROSIA
FOREST PEST
METAGENOMIC
FUNGI
PLATYPODINAE
INSECT-FUNGUS INTERACTION
title_short Metagenomic approach of associated fungi with megaplatypus mutatus (Coleoptera: Platypodinae)
title_full Metagenomic approach of associated fungi with megaplatypus mutatus (Coleoptera: Platypodinae)
title_fullStr Metagenomic approach of associated fungi with megaplatypus mutatus (Coleoptera: Platypodinae)
title_full_unstemmed Metagenomic approach of associated fungi with megaplatypus mutatus (Coleoptera: Platypodinae)
title_sort Metagenomic approach of associated fungi with megaplatypus mutatus (Coleoptera: Platypodinae)
dc.creator.none.fl_str_mv Ceriani Nakamurakare, Esteban Daniel
Ramos, Sergio
Robles, Carolina Analía
Novas, María Victoria
D'jonsiles, María Fernanda
Gonzalez Audino, Paola Andrea
Carmaran, Cecilia Cristina
author Ceriani Nakamurakare, Esteban Daniel
author_facet Ceriani Nakamurakare, Esteban Daniel
Ramos, Sergio
Robles, Carolina Analía
Novas, María Victoria
D'jonsiles, María Fernanda
Gonzalez Audino, Paola Andrea
Carmaran, Cecilia Cristina
author_role author
author2 Ramos, Sergio
Robles, Carolina Analía
Novas, María Victoria
D'jonsiles, María Fernanda
Gonzalez Audino, Paola Andrea
Carmaran, Cecilia Cristina
author2_role author
author
author
author
author
author
dc.subject.none.fl_str_mv AMBROSIA
FOREST PEST
METAGENOMIC
FUNGI
PLATYPODINAE
INSECT-FUNGUS INTERACTION
topic AMBROSIA
FOREST PEST
METAGENOMIC
FUNGI
PLATYPODINAE
INSECT-FUNGUS INTERACTION
purl_subject.fl_str_mv https://purl.org/becyt/ford/1.6
https://purl.org/becyt/ford/1
https://purl.org/becyt/ford/1.6
https://purl.org/becyt/ford/1
dc.description.none.fl_txt_mv Megaplatypus mutatus is a major forestpest in Argentina and an emerging pest in Europe. In this study the multitrophic interactions between M. mutatus and associated fungi were assessed with ametagenomics approach (454-pyrosequencing). A total of 270 collection pointsfrom insect galleries from three locations in Argentina were pooled for pyrosequencing analyses. Two hosts, Populus deltoides and Casuarina cunninghamiana, were independently evaluated to characterize the fungal communities associated to M.mutatus; compare the culture-independent approach with previous culturingstudies, in terms of data recovery related to the fungal community composition,and test the specificity of the fungal communities amongst locations and hosts. A Generalized Linear Mixed Model was performed to compare the fungal richnessin each dataset, which showed no significant differences between taxa richness amongst locations. Principal Coordinates Analysesshowed a separation between fungal communities within the same host, suggesting that host identity would not be crucial to determine the specificity in fungal communities. Candida insectalens and one Fusarium species, present in all hosts and locations, achieved 37.6 % of the total relative frequency per taxa. These results complement the data from culturing methods previously reported, thus improving the accuracy and understanding of the fungal assemblages associated to M. mutatus.
Fil: Ceriani Nakamurakare, Esteban Daniel. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales. Departamento de Biodiversidad y Biología Experimental; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina
Fil: Ramos, Sergio. Instituto Nacional de Tecnologia Agropecuaria. Centro Regional Entre Rios. Estacion Experimental Agropecuaria Concordia.; Argentina
Fil: Robles, Carolina Analía. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales. Departamento de Biodiversidad y Biología Experimental; Argentina
Fil: Novas, María Victoria. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Ciudad Universitaria. Instituto de Micología y Botánica. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales. Instituto de Micología y Botánica; Argentina
Fil: D'jonsiles, María Fernanda. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales. Departamento de Biodiversidad y Biología Experimental; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina
Fil: Gonzalez Audino, Paola Andrea. Consejo Nacional de Investigaciones Científicas y Técnicas. Instituto de Investigaciones Científicas y Técnicas para la Defensa. Centro de Investigación de Plagas e Insecticidas; Argentina
Fil: Carmaran, Cecilia Cristina. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales. Departamento de Biodiversidad y Biología Experimental; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina
description Megaplatypus mutatus is a major forestpest in Argentina and an emerging pest in Europe. In this study the multitrophic interactions between M. mutatus and associated fungi were assessed with ametagenomics approach (454-pyrosequencing). A total of 270 collection pointsfrom insect galleries from three locations in Argentina were pooled for pyrosequencing analyses. Two hosts, Populus deltoides and Casuarina cunninghamiana, were independently evaluated to characterize the fungal communities associated to M.mutatus; compare the culture-independent approach with previous culturingstudies, in terms of data recovery related to the fungal community composition,and test the specificity of the fungal communities amongst locations and hosts. A Generalized Linear Mixed Model was performed to compare the fungal richnessin each dataset, which showed no significant differences between taxa richness amongst locations. Principal Coordinates Analysesshowed a separation between fungal communities within the same host, suggesting that host identity would not be crucial to determine the specificity in fungal communities. Candida insectalens and one Fusarium species, present in all hosts and locations, achieved 37.6 % of the total relative frequency per taxa. These results complement the data from culturing methods previously reported, thus improving the accuracy and understanding of the fungal assemblages associated to M. mutatus.
publishDate 2018
dc.date.none.fl_str_mv 2018-05
dc.type.none.fl_str_mv info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
http://purl.org/coar/resource_type/c_6501
info:ar-repo/semantics/articulo
format article
status_str publishedVersion
dc.identifier.none.fl_str_mv http://hdl.handle.net/11336/160559
Ceriani Nakamurakare, Esteban Daniel; Ramos, Sergio; Robles, Carolina Analía; Novas, María Victoria; D'jonsiles, María Fernanda; et al.; Metagenomic approach of associated fungi with megaplatypus mutatus (Coleoptera: Platypodinae); Finnish Soc Forest Sciencefinnish Forest Research; Silva Fennica; 52; 3; 5-2018; 1-16
0037-5330
CONICET Digital
CONICET
url http://hdl.handle.net/11336/160559
identifier_str_mv Ceriani Nakamurakare, Esteban Daniel; Ramos, Sergio; Robles, Carolina Analía; Novas, María Victoria; D'jonsiles, María Fernanda; et al.; Metagenomic approach of associated fungi with megaplatypus mutatus (Coleoptera: Platypodinae); Finnish Soc Forest Sciencefinnish Forest Research; Silva Fennica; 52; 3; 5-2018; 1-16
0037-5330
CONICET Digital
CONICET
dc.language.none.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv info:eu-repo/semantics/altIdentifier/url/https://www.silvafennica.fi/pdf/article9940.pdf
info:eu-repo/semantics/altIdentifier/doi/10.14214/sf.9940
info:eu-repo/semantics/altIdentifier/url/https://www.silvafennica.fi/article/9940
dc.rights.none.fl_str_mv info:eu-repo/semantics/openAccess
https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
eu_rights_str_mv openAccess
rights_invalid_str_mv https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
dc.format.none.fl_str_mv application/pdf
application/pdf
application/pdf
dc.publisher.none.fl_str_mv Finnish Soc Forest Sciencefinnish Forest Research
publisher.none.fl_str_mv Finnish Soc Forest Sciencefinnish Forest Research
dc.source.none.fl_str_mv reponame:CONICET Digital (CONICET)
instname:Consejo Nacional de Investigaciones Científicas y Técnicas
reponame_str CONICET Digital (CONICET)
collection CONICET Digital (CONICET)
instname_str Consejo Nacional de Investigaciones Científicas y Técnicas
repository.name.fl_str_mv CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicas
repository.mail.fl_str_mv dasensio@conicet.gov.ar; lcarlino@conicet.gov.ar
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score 12.982451

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