Metagenomic approach of associated fungi with megaplatypus mutatus (Coleoptera: Platypodinae)
- Autores
- Ceriani Nakamurakare, Esteban Daniel; Ramos, Sergio; Robles, Carolina Analía; Novas, María Victoria; D'jonsiles, María Fernanda; Gonzalez Audino, Paola Andrea; Carmaran, Cecilia Cristina
- Año de publicación
- 2018
- Idioma
- inglés
- Tipo de recurso
- artículo
- Estado
- versión publicada
- Descripción
- Megaplatypus mutatus is a major forestpest in Argentina and an emerging pest in Europe. In this study the multitrophic interactions between M. mutatus and associated fungi were assessed with ametagenomics approach (454-pyrosequencing). A total of 270 collection pointsfrom insect galleries from three locations in Argentina were pooled for pyrosequencing analyses. Two hosts, Populus deltoides and Casuarina cunninghamiana, were independently evaluated to characterize the fungal communities associated to M.mutatus; compare the culture-independent approach with previous culturingstudies, in terms of data recovery related to the fungal community composition,and test the specificity of the fungal communities amongst locations and hosts. A Generalized Linear Mixed Model was performed to compare the fungal richnessin each dataset, which showed no significant differences between taxa richness amongst locations. Principal Coordinates Analysesshowed a separation between fungal communities within the same host, suggesting that host identity would not be crucial to determine the specificity in fungal communities. Candida insectalens and one Fusarium species, present in all hosts and locations, achieved 37.6 % of the total relative frequency per taxa. These results complement the data from culturing methods previously reported, thus improving the accuracy and understanding of the fungal assemblages associated to M. mutatus.
Fil: Ceriani Nakamurakare, Esteban Daniel. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales. Departamento de Biodiversidad y Biología Experimental; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina
Fil: Ramos, Sergio. Instituto Nacional de Tecnologia Agropecuaria. Centro Regional Entre Rios. Estacion Experimental Agropecuaria Concordia.; Argentina
Fil: Robles, Carolina Analía. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales. Departamento de Biodiversidad y Biología Experimental; Argentina
Fil: Novas, María Victoria. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Ciudad Universitaria. Instituto de Micología y Botánica. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales. Instituto de Micología y Botánica; Argentina
Fil: D'jonsiles, María Fernanda. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales. Departamento de Biodiversidad y Biología Experimental; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina
Fil: Gonzalez Audino, Paola Andrea. Consejo Nacional de Investigaciones Científicas y Técnicas. Instituto de Investigaciones Científicas y Técnicas para la Defensa. Centro de Investigación de Plagas e Insecticidas; Argentina
Fil: Carmaran, Cecilia Cristina. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales. Departamento de Biodiversidad y Biología Experimental; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina - Materia
-
AMBROSIA
FOREST PEST
METAGENOMIC
FUNGI
PLATYPODINAE
INSECT-FUNGUS INTERACTION - Nivel de accesibilidad
- acceso abierto
- Condiciones de uso
- https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
- Repositorio
- Institución
- Consejo Nacional de Investigaciones Científicas y Técnicas
- OAI Identificador
- oai:ri.conicet.gov.ar:11336/160559
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CONICET Digital (CONICET) |
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Metagenomic approach of associated fungi with megaplatypus mutatus (Coleoptera: Platypodinae)Ceriani Nakamurakare, Esteban DanielRamos, SergioRobles, Carolina AnalíaNovas, María VictoriaD'jonsiles, María FernandaGonzalez Audino, Paola AndreaCarmaran, Cecilia CristinaAMBROSIAFOREST PESTMETAGENOMICFUNGIPLATYPODINAEINSECT-FUNGUS INTERACTIONhttps://purl.org/becyt/ford/1.6https://purl.org/becyt/ford/1https://purl.org/becyt/ford/1.6https://purl.org/becyt/ford/1Megaplatypus mutatus is a major forestpest in Argentina and an emerging pest in Europe. In this study the multitrophic interactions between M. mutatus and associated fungi were assessed with ametagenomics approach (454-pyrosequencing). A total of 270 collection pointsfrom insect galleries from three locations in Argentina were pooled for pyrosequencing analyses. Two hosts, Populus deltoides and Casuarina cunninghamiana, were independently evaluated to characterize the fungal communities associated to M.mutatus; compare the culture-independent approach with previous culturingstudies, in terms of data recovery related to the fungal community composition,and test the specificity of the fungal communities amongst locations and hosts. A Generalized Linear Mixed Model was performed to compare the fungal richnessin each dataset, which showed no significant differences between taxa richness amongst locations. Principal Coordinates Analysesshowed a separation between fungal communities within the same host, suggesting that host identity would not be crucial to determine the specificity in fungal communities. Candida insectalens and one Fusarium species, present in all hosts and locations, achieved 37.6 % of the total relative frequency per taxa. These results complement the data from culturing methods previously reported, thus improving the accuracy and understanding of the fungal assemblages associated to M. mutatus.Fil: Ceriani Nakamurakare, Esteban Daniel. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales. Departamento de Biodiversidad y Biología Experimental; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; ArgentinaFil: Ramos, Sergio. Instituto Nacional de Tecnologia Agropecuaria. Centro Regional Entre Rios. Estacion Experimental Agropecuaria Concordia.; ArgentinaFil: Robles, Carolina Analía. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales. Departamento de Biodiversidad y Biología Experimental; ArgentinaFil: Novas, María Victoria. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Ciudad Universitaria. Instituto de Micología y Botánica. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales. Instituto de Micología y Botánica; ArgentinaFil: D'jonsiles, María Fernanda. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales. Departamento de Biodiversidad y Biología Experimental; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; ArgentinaFil: Gonzalez Audino, Paola Andrea. Consejo Nacional de Investigaciones Científicas y Técnicas. Instituto de Investigaciones Científicas y Técnicas para la Defensa. Centro de Investigación de Plagas e Insecticidas; ArgentinaFil: Carmaran, Cecilia Cristina. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales. Departamento de Biodiversidad y Biología Experimental; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; ArgentinaFinnish Soc Forest Sciencefinnish Forest Research2018-05info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionhttp://purl.org/coar/resource_type/c_6501info:ar-repo/semantics/articuloapplication/pdfapplication/pdfapplication/pdfhttp://hdl.handle.net/11336/160559Ceriani Nakamurakare, Esteban Daniel; Ramos, Sergio; Robles, Carolina Analía; Novas, María Victoria; D'jonsiles, María Fernanda; et al.; Metagenomic approach of associated fungi with megaplatypus mutatus (Coleoptera: Platypodinae); Finnish Soc Forest Sciencefinnish Forest Research; Silva Fennica; 52; 3; 5-2018; 1-160037-5330CONICET DigitalCONICETenginfo:eu-repo/semantics/altIdentifier/url/https://www.silvafennica.fi/pdf/article9940.pdfinfo:eu-repo/semantics/altIdentifier/doi/10.14214/sf.9940info:eu-repo/semantics/altIdentifier/url/https://www.silvafennica.fi/article/9940info:eu-repo/semantics/openAccesshttps://creativecommons.org/licenses/by-nc-sa/2.5/ar/reponame:CONICET Digital (CONICET)instname:Consejo Nacional de Investigaciones Científicas y Técnicas2025-10-22T11:53:55Zoai:ri.conicet.gov.ar:11336/160559instacron:CONICETInstitucionalhttp://ri.conicet.gov.ar/Organismo científico-tecnológicoNo correspondehttp://ri.conicet.gov.ar/oai/requestdasensio@conicet.gov.ar; lcarlino@conicet.gov.arArgentinaNo correspondeNo correspondeNo correspondeopendoar:34982025-10-22 11:53:55.932CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicasfalse |
dc.title.none.fl_str_mv |
Metagenomic approach of associated fungi with megaplatypus mutatus (Coleoptera: Platypodinae) |
title |
Metagenomic approach of associated fungi with megaplatypus mutatus (Coleoptera: Platypodinae) |
spellingShingle |
Metagenomic approach of associated fungi with megaplatypus mutatus (Coleoptera: Platypodinae) Ceriani Nakamurakare, Esteban Daniel AMBROSIA FOREST PEST METAGENOMIC FUNGI PLATYPODINAE INSECT-FUNGUS INTERACTION |
title_short |
Metagenomic approach of associated fungi with megaplatypus mutatus (Coleoptera: Platypodinae) |
title_full |
Metagenomic approach of associated fungi with megaplatypus mutatus (Coleoptera: Platypodinae) |
title_fullStr |
Metagenomic approach of associated fungi with megaplatypus mutatus (Coleoptera: Platypodinae) |
title_full_unstemmed |
Metagenomic approach of associated fungi with megaplatypus mutatus (Coleoptera: Platypodinae) |
title_sort |
Metagenomic approach of associated fungi with megaplatypus mutatus (Coleoptera: Platypodinae) |
dc.creator.none.fl_str_mv |
Ceriani Nakamurakare, Esteban Daniel Ramos, Sergio Robles, Carolina Analía Novas, María Victoria D'jonsiles, María Fernanda Gonzalez Audino, Paola Andrea Carmaran, Cecilia Cristina |
author |
Ceriani Nakamurakare, Esteban Daniel |
author_facet |
Ceriani Nakamurakare, Esteban Daniel Ramos, Sergio Robles, Carolina Analía Novas, María Victoria D'jonsiles, María Fernanda Gonzalez Audino, Paola Andrea Carmaran, Cecilia Cristina |
author_role |
author |
author2 |
Ramos, Sergio Robles, Carolina Analía Novas, María Victoria D'jonsiles, María Fernanda Gonzalez Audino, Paola Andrea Carmaran, Cecilia Cristina |
author2_role |
author author author author author author |
dc.subject.none.fl_str_mv |
AMBROSIA FOREST PEST METAGENOMIC FUNGI PLATYPODINAE INSECT-FUNGUS INTERACTION |
topic |
AMBROSIA FOREST PEST METAGENOMIC FUNGI PLATYPODINAE INSECT-FUNGUS INTERACTION |
purl_subject.fl_str_mv |
https://purl.org/becyt/ford/1.6 https://purl.org/becyt/ford/1 https://purl.org/becyt/ford/1.6 https://purl.org/becyt/ford/1 |
dc.description.none.fl_txt_mv |
Megaplatypus mutatus is a major forestpest in Argentina and an emerging pest in Europe. In this study the multitrophic interactions between M. mutatus and associated fungi were assessed with ametagenomics approach (454-pyrosequencing). A total of 270 collection pointsfrom insect galleries from three locations in Argentina were pooled for pyrosequencing analyses. Two hosts, Populus deltoides and Casuarina cunninghamiana, were independently evaluated to characterize the fungal communities associated to M.mutatus; compare the culture-independent approach with previous culturingstudies, in terms of data recovery related to the fungal community composition,and test the specificity of the fungal communities amongst locations and hosts. A Generalized Linear Mixed Model was performed to compare the fungal richnessin each dataset, which showed no significant differences between taxa richness amongst locations. Principal Coordinates Analysesshowed a separation between fungal communities within the same host, suggesting that host identity would not be crucial to determine the specificity in fungal communities. Candida insectalens and one Fusarium species, present in all hosts and locations, achieved 37.6 % of the total relative frequency per taxa. These results complement the data from culturing methods previously reported, thus improving the accuracy and understanding of the fungal assemblages associated to M. mutatus. Fil: Ceriani Nakamurakare, Esteban Daniel. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales. Departamento de Biodiversidad y Biología Experimental; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina Fil: Ramos, Sergio. Instituto Nacional de Tecnologia Agropecuaria. Centro Regional Entre Rios. Estacion Experimental Agropecuaria Concordia.; Argentina Fil: Robles, Carolina Analía. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales. Departamento de Biodiversidad y Biología Experimental; Argentina Fil: Novas, María Victoria. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Ciudad Universitaria. Instituto de Micología y Botánica. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales. Instituto de Micología y Botánica; Argentina Fil: D'jonsiles, María Fernanda. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales. Departamento de Biodiversidad y Biología Experimental; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina Fil: Gonzalez Audino, Paola Andrea. Consejo Nacional de Investigaciones Científicas y Técnicas. Instituto de Investigaciones Científicas y Técnicas para la Defensa. Centro de Investigación de Plagas e Insecticidas; Argentina Fil: Carmaran, Cecilia Cristina. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales. Departamento de Biodiversidad y Biología Experimental; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina |
description |
Megaplatypus mutatus is a major forestpest in Argentina and an emerging pest in Europe. In this study the multitrophic interactions between M. mutatus and associated fungi were assessed with ametagenomics approach (454-pyrosequencing). A total of 270 collection pointsfrom insect galleries from three locations in Argentina were pooled for pyrosequencing analyses. Two hosts, Populus deltoides and Casuarina cunninghamiana, were independently evaluated to characterize the fungal communities associated to M.mutatus; compare the culture-independent approach with previous culturingstudies, in terms of data recovery related to the fungal community composition,and test the specificity of the fungal communities amongst locations and hosts. A Generalized Linear Mixed Model was performed to compare the fungal richnessin each dataset, which showed no significant differences between taxa richness amongst locations. Principal Coordinates Analysesshowed a separation between fungal communities within the same host, suggesting that host identity would not be crucial to determine the specificity in fungal communities. Candida insectalens and one Fusarium species, present in all hosts and locations, achieved 37.6 % of the total relative frequency per taxa. These results complement the data from culturing methods previously reported, thus improving the accuracy and understanding of the fungal assemblages associated to M. mutatus. |
publishDate |
2018 |
dc.date.none.fl_str_mv |
2018-05 |
dc.type.none.fl_str_mv |
info:eu-repo/semantics/article info:eu-repo/semantics/publishedVersion http://purl.org/coar/resource_type/c_6501 info:ar-repo/semantics/articulo |
format |
article |
status_str |
publishedVersion |
dc.identifier.none.fl_str_mv |
http://hdl.handle.net/11336/160559 Ceriani Nakamurakare, Esteban Daniel; Ramos, Sergio; Robles, Carolina Analía; Novas, María Victoria; D'jonsiles, María Fernanda; et al.; Metagenomic approach of associated fungi with megaplatypus mutatus (Coleoptera: Platypodinae); Finnish Soc Forest Sciencefinnish Forest Research; Silva Fennica; 52; 3; 5-2018; 1-16 0037-5330 CONICET Digital CONICET |
url |
http://hdl.handle.net/11336/160559 |
identifier_str_mv |
Ceriani Nakamurakare, Esteban Daniel; Ramos, Sergio; Robles, Carolina Analía; Novas, María Victoria; D'jonsiles, María Fernanda; et al.; Metagenomic approach of associated fungi with megaplatypus mutatus (Coleoptera: Platypodinae); Finnish Soc Forest Sciencefinnish Forest Research; Silva Fennica; 52; 3; 5-2018; 1-16 0037-5330 CONICET Digital CONICET |
dc.language.none.fl_str_mv |
eng |
language |
eng |
dc.relation.none.fl_str_mv |
info:eu-repo/semantics/altIdentifier/url/https://www.silvafennica.fi/pdf/article9940.pdf info:eu-repo/semantics/altIdentifier/doi/10.14214/sf.9940 info:eu-repo/semantics/altIdentifier/url/https://www.silvafennica.fi/article/9940 |
dc.rights.none.fl_str_mv |
info:eu-repo/semantics/openAccess https://creativecommons.org/licenses/by-nc-sa/2.5/ar/ |
eu_rights_str_mv |
openAccess |
rights_invalid_str_mv |
https://creativecommons.org/licenses/by-nc-sa/2.5/ar/ |
dc.format.none.fl_str_mv |
application/pdf application/pdf application/pdf |
dc.publisher.none.fl_str_mv |
Finnish Soc Forest Sciencefinnish Forest Research |
publisher.none.fl_str_mv |
Finnish Soc Forest Sciencefinnish Forest Research |
dc.source.none.fl_str_mv |
reponame:CONICET Digital (CONICET) instname:Consejo Nacional de Investigaciones Científicas y Técnicas |
reponame_str |
CONICET Digital (CONICET) |
collection |
CONICET Digital (CONICET) |
instname_str |
Consejo Nacional de Investigaciones Científicas y Técnicas |
repository.name.fl_str_mv |
CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicas |
repository.mail.fl_str_mv |
dasensio@conicet.gov.ar; lcarlino@conicet.gov.ar |
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1846782236317188096 |
score |
12.928904 |