Effect of the exposure with sublethal dosis to the insecticides Imidacloprid. Bifenthrin andChlorpyrifos or its combination on the total amount of body proteins and mRNA expression...

Autores
Sarlo, Edgardo; Quintana, Silvina; Perezlindo, Tamara; Iglesias, Azucena Elizabeth; Iturburu, Fernando Gastón; Menone, Mirta Lujan; Eguaras, Martin Javier; Medici, Sandra Karina
Año de publicación
2023
Idioma
inglés
Tipo de recurso
documento de conferencia
Estado
versión publicada
Descripción
Agrochemicals, and particularly insecticides in sublethal doses, are widely recognized as being among the group of stressorsthat drastically reduce the fitness of insects. Particularly, Apis mellifera is known for its association with agriculture to sublethalcontact with a wide range of this group of stressors that trigger different types of stress, such as nutritional stress. Based onthis, the aim of this research is to determine if the level of total body proteins and the expression of the Vitellogenin gene inthree days emerged bees varies before the topical exposure to a sublethal dose (LD10) of the pesticides Imidacloprid.Bifenthrin and Chlorpyrifos or a combination of pairs of them. The bioassay was performed on a total of 30 A mellifera workersper treatment, which were contaminated with each pesticide or combination of them and after 24 hours were sacrificed withliquid nitrogen. Total protein content was obtained according to Bradford (1976) while Vitelogenin mRNA levels weredetermined by RT-qPCR. No significant differences were observed in the total protein content between the control group andthe individual treatment groups with bifenthrin and chlorpyrifos, however in the rest of the treatments the total protein contentwas significantly lower than the control. A significantly difference in mRNA levels was also found between individuals in theuntreated group and all of the treated groups. Within the treated groups, the mRNA expression in the group in contact withbifenthrin was significantly higher than among all other groups. It was concluded that the individuals that were in contact withthe agrochemicals studied presented a lower synthesis of vitellogenin mRNA, which may coincide with studies in other insectspecies. While it was postulated that this type of agrochemicals to a greater or lesser degree, depending on the familystimulates the corpora allata generating an increase in the juvenile hormone and therefore, in the case of bees, an inhibition ofthe synthesis of vitellogenin.
Fil: Sarlo, Edgardo. Universidad Nacional de Mar del Plata. Instituto de Investigaciones En Produccion, Sanidad y Ambiente. - Consejo Nacional de Investigaciones Cientificas y Tecnicas. Centro Cientifico Tecnologico Conicet - Mar del Plata. Instituto de Investigaciones En Produccion, Sanidad y Ambiente.; Argentina
Fil: Quintana, Silvina. Universidad Nacional de Mar del Plata. Instituto de Investigaciones En Produccion, Sanidad y Ambiente. - Consejo Nacional de Investigaciones Cientificas y Tecnicas. Centro Cientifico Tecnologico Conicet - Mar del Plata. Instituto de Investigaciones En Produccion, Sanidad y Ambiente.; Argentina
Fil: Perezlindo, Tamara. Fares Taie Biotecnología; Argentina
Fil: Iglesias, Azucena Elizabeth. Universidad Nacional de Mar del Plata. Instituto de Investigaciones En Produccion, Sanidad y Ambiente. - Consejo Nacional de Investigaciones Cientificas y Tecnicas. Centro Cientifico Tecnologico Conicet - Mar del Plata. Instituto de Investigaciones En Produccion, Sanidad y Ambiente.; Argentina
Fil: Iturburu, Fernando Gastón. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Mar del Plata. Instituto de Investigaciones Marinas y Costeras. Universidad Nacional de Mar del Plata. Facultad de Ciencias Exactas y Naturales. Instituto de Investigaciones Marinas y Costeras; Argentina
Fil: Menone, Mirta Lujan. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Mar del Plata. Instituto de Investigaciones Marinas y Costeras. Universidad Nacional de Mar del Plata. Facultad de Ciencias Exactas y Naturales. Instituto de Investigaciones Marinas y Costeras; Argentina
Fil: Eguaras, Martin Javier. Universidad Nacional de Mar del Plata. Instituto de Investigaciones En Produccion, Sanidad y Ambiente. - Consejo Nacional de Investigaciones Cientificas y Tecnicas. Centro Cientifico Tecnologico Conicet - Mar del Plata. Instituto de Investigaciones En Produccion, Sanidad y Ambiente.; Argentina
Fil: Medici, Sandra Karina. Universidad Nacional de Mar del Plata. Instituto de Investigaciones En Produccion, Sanidad y Ambiente. - Consejo Nacional de Investigaciones Cientificas y Tecnicas. Centro Cientifico Tecnologico Conicet - Mar del Plata. Instituto de Investigaciones En Produccion, Sanidad y Ambiente.; Argentina
48th International Apicultural Congress
Santiago de Chile
Chile
Federación Internacional de Asociaciones de Apicultura
Materia
Honey Bees
pesticides
protein
Vitellogenin
Nivel de accesibilidad
acceso abierto
Condiciones de uso
https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
Repositorio
CONICET Digital (CONICET)
Institución
Consejo Nacional de Investigaciones Científicas y Técnicas
OAI Identificador
oai:ri.conicet.gov.ar:11336/250436
Acceder
id CONICETDig_12ea72b2036f10f37bf92addc4e45332
oai_identifier_str oai:ri.conicet.gov.ar:11336/250436
network_acronym_str CONICETDig
repository_id_str 3498
network_name_str CONICET Digital (CONICET)
spelling Effect of the exposure with sublethal dosis to the insecticides Imidacloprid. Bifenthrin andChlorpyrifos or its combination on the total amount of body proteins and mRNA expression levels ofVitelogenin on Apis mellifera workers beesSarlo, EdgardoQuintana, SilvinaPerezlindo, TamaraIglesias, Azucena ElizabethIturburu, Fernando GastónMenone, Mirta LujanEguaras, Martin JavierMedici, Sandra KarinaHoney BeespesticidesproteinVitellogeninhttps://purl.org/becyt/ford/1.6https://purl.org/becyt/ford/1Agrochemicals, and particularly insecticides in sublethal doses, are widely recognized as being among the group of stressorsthat drastically reduce the fitness of insects. Particularly, Apis mellifera is known for its association with agriculture to sublethalcontact with a wide range of this group of stressors that trigger different types of stress, such as nutritional stress. Based onthis, the aim of this research is to determine if the level of total body proteins and the expression of the Vitellogenin gene inthree days emerged bees varies before the topical exposure to a sublethal dose (LD10) of the pesticides Imidacloprid.Bifenthrin and Chlorpyrifos or a combination of pairs of them. The bioassay was performed on a total of 30 A mellifera workersper treatment, which were contaminated with each pesticide or combination of them and after 24 hours were sacrificed withliquid nitrogen. Total protein content was obtained according to Bradford (1976) while Vitelogenin mRNA levels weredetermined by RT-qPCR. No significant differences were observed in the total protein content between the control group andthe individual treatment groups with bifenthrin and chlorpyrifos, however in the rest of the treatments the total protein contentwas significantly lower than the control. A significantly difference in mRNA levels was also found between individuals in theuntreated group and all of the treated groups. Within the treated groups, the mRNA expression in the group in contact withbifenthrin was significantly higher than among all other groups. It was concluded that the individuals that were in contact withthe agrochemicals studied presented a lower synthesis of vitellogenin mRNA, which may coincide with studies in other insectspecies. While it was postulated that this type of agrochemicals to a greater or lesser degree, depending on the familystimulates the corpora allata generating an increase in the juvenile hormone and therefore, in the case of bees, an inhibition ofthe synthesis of vitellogenin.Fil: Sarlo, Edgardo. Universidad Nacional de Mar del Plata. Instituto de Investigaciones En Produccion, Sanidad y Ambiente. - Consejo Nacional de Investigaciones Cientificas y Tecnicas. Centro Cientifico Tecnologico Conicet - Mar del Plata. Instituto de Investigaciones En Produccion, Sanidad y Ambiente.; ArgentinaFil: Quintana, Silvina. Universidad Nacional de Mar del Plata. Instituto de Investigaciones En Produccion, Sanidad y Ambiente. - Consejo Nacional de Investigaciones Cientificas y Tecnicas. Centro Cientifico Tecnologico Conicet - Mar del Plata. Instituto de Investigaciones En Produccion, Sanidad y Ambiente.; ArgentinaFil: Perezlindo, Tamara. Fares Taie Biotecnología; ArgentinaFil: Iglesias, Azucena Elizabeth. Universidad Nacional de Mar del Plata. Instituto de Investigaciones En Produccion, Sanidad y Ambiente. - Consejo Nacional de Investigaciones Cientificas y Tecnicas. Centro Cientifico Tecnologico Conicet - Mar del Plata. Instituto de Investigaciones En Produccion, Sanidad y Ambiente.; ArgentinaFil: Iturburu, Fernando Gastón. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Mar del Plata. Instituto de Investigaciones Marinas y Costeras. Universidad Nacional de Mar del Plata. Facultad de Ciencias Exactas y Naturales. Instituto de Investigaciones Marinas y Costeras; ArgentinaFil: Menone, Mirta Lujan. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Mar del Plata. Instituto de Investigaciones Marinas y Costeras. Universidad Nacional de Mar del Plata. Facultad de Ciencias Exactas y Naturales. Instituto de Investigaciones Marinas y Costeras; ArgentinaFil: Eguaras, Martin Javier. Universidad Nacional de Mar del Plata. Instituto de Investigaciones En Produccion, Sanidad y Ambiente. - Consejo Nacional de Investigaciones Cientificas y Tecnicas. Centro Cientifico Tecnologico Conicet - Mar del Plata. Instituto de Investigaciones En Produccion, Sanidad y Ambiente.; ArgentinaFil: Medici, Sandra Karina. Universidad Nacional de Mar del Plata. Instituto de Investigaciones En Produccion, Sanidad y Ambiente. - Consejo Nacional de Investigaciones Cientificas y Tecnicas. Centro Cientifico Tecnologico Conicet - Mar del Plata. Instituto de Investigaciones En Produccion, Sanidad y Ambiente.; Argentina48th International Apicultural CongressSantiago de ChileChileFederación Internacional de Asociaciones de ApiculturaConmark Tourism & Event Management Company2023info:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/conferenceObjectCongresoBookhttp://purl.org/coar/resource_type/c_5794info:ar-repo/semantics/documentoDeConferenciaapplication/pdfapplication/pdfapplication/pdfhttp://hdl.handle.net/11336/250436Effect of the exposure with sublethal dosis to the insecticides Imidacloprid. Bifenthrin andChlorpyrifos or its combination on the total amount of body proteins and mRNA expression levels ofVitelogenin on Apis mellifera workers bees; 48th International Apicultural Congress; Santiago de Chile; Chile; 2023; 265-265CONICET DigitalCONICETenginfo:eu-repo/semantics/altIdentifier/url/https://apimondia2023.com/docs/abstract-book.pdfInternacionalinfo:eu-repo/semantics/openAccesshttps://creativecommons.org/licenses/by-nc-sa/2.5/ar/reponame:CONICET Digital (CONICET)instname:Consejo Nacional de Investigaciones Científicas y Técnicas2025-09-29T09:39:17Zoai:ri.conicet.gov.ar:11336/250436instacron:CONICETInstitucionalhttp://ri.conicet.gov.ar/Organismo científico-tecnológicoNo correspondehttp://ri.conicet.gov.ar/oai/requestdasensio@conicet.gov.ar; lcarlino@conicet.gov.arArgentinaNo correspondeNo correspondeNo correspondeopendoar:34982025-09-29 09:39:17.885CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicasfalse
dc.title.none.fl_str_mv Effect of the exposure with sublethal dosis to the insecticides Imidacloprid. Bifenthrin andChlorpyrifos or its combination on the total amount of body proteins and mRNA expression levels ofVitelogenin on Apis mellifera workers bees
title Effect of the exposure with sublethal dosis to the insecticides Imidacloprid. Bifenthrin andChlorpyrifos or its combination on the total amount of body proteins and mRNA expression levels ofVitelogenin on Apis mellifera workers bees
spellingShingle Effect of the exposure with sublethal dosis to the insecticides Imidacloprid. Bifenthrin andChlorpyrifos or its combination on the total amount of body proteins and mRNA expression levels ofVitelogenin on Apis mellifera workers bees
Sarlo, Edgardo
Honey Bees
pesticides
protein
Vitellogenin
title_short Effect of the exposure with sublethal dosis to the insecticides Imidacloprid. Bifenthrin andChlorpyrifos or its combination on the total amount of body proteins and mRNA expression levels ofVitelogenin on Apis mellifera workers bees
title_full Effect of the exposure with sublethal dosis to the insecticides Imidacloprid. Bifenthrin andChlorpyrifos or its combination on the total amount of body proteins and mRNA expression levels ofVitelogenin on Apis mellifera workers bees
title_fullStr Effect of the exposure with sublethal dosis to the insecticides Imidacloprid. Bifenthrin andChlorpyrifos or its combination on the total amount of body proteins and mRNA expression levels ofVitelogenin on Apis mellifera workers bees
title_full_unstemmed Effect of the exposure with sublethal dosis to the insecticides Imidacloprid. Bifenthrin andChlorpyrifos or its combination on the total amount of body proteins and mRNA expression levels ofVitelogenin on Apis mellifera workers bees
title_sort Effect of the exposure with sublethal dosis to the insecticides Imidacloprid. Bifenthrin andChlorpyrifos or its combination on the total amount of body proteins and mRNA expression levels ofVitelogenin on Apis mellifera workers bees
dc.creator.none.fl_str_mv Sarlo, Edgardo
Quintana, Silvina
Perezlindo, Tamara
Iglesias, Azucena Elizabeth
Iturburu, Fernando Gastón
Menone, Mirta Lujan
Eguaras, Martin Javier
Medici, Sandra Karina
author Sarlo, Edgardo
author_facet Sarlo, Edgardo
Quintana, Silvina
Perezlindo, Tamara
Iglesias, Azucena Elizabeth
Iturburu, Fernando Gastón
Menone, Mirta Lujan
Eguaras, Martin Javier
Medici, Sandra Karina
author_role author
author2 Quintana, Silvina
Perezlindo, Tamara
Iglesias, Azucena Elizabeth
Iturburu, Fernando Gastón
Menone, Mirta Lujan
Eguaras, Martin Javier
Medici, Sandra Karina
author2_role author
author
author
author
author
author
author
dc.subject.none.fl_str_mv Honey Bees
pesticides
protein
Vitellogenin
topic Honey Bees
pesticides
protein
Vitellogenin
purl_subject.fl_str_mv https://purl.org/becyt/ford/1.6
https://purl.org/becyt/ford/1
dc.description.none.fl_txt_mv Agrochemicals, and particularly insecticides in sublethal doses, are widely recognized as being among the group of stressorsthat drastically reduce the fitness of insects. Particularly, Apis mellifera is known for its association with agriculture to sublethalcontact with a wide range of this group of stressors that trigger different types of stress, such as nutritional stress. Based onthis, the aim of this research is to determine if the level of total body proteins and the expression of the Vitellogenin gene inthree days emerged bees varies before the topical exposure to a sublethal dose (LD10) of the pesticides Imidacloprid.Bifenthrin and Chlorpyrifos or a combination of pairs of them. The bioassay was performed on a total of 30 A mellifera workersper treatment, which were contaminated with each pesticide or combination of them and after 24 hours were sacrificed withliquid nitrogen. Total protein content was obtained according to Bradford (1976) while Vitelogenin mRNA levels weredetermined by RT-qPCR. No significant differences were observed in the total protein content between the control group andthe individual treatment groups with bifenthrin and chlorpyrifos, however in the rest of the treatments the total protein contentwas significantly lower than the control. A significantly difference in mRNA levels was also found between individuals in theuntreated group and all of the treated groups. Within the treated groups, the mRNA expression in the group in contact withbifenthrin was significantly higher than among all other groups. It was concluded that the individuals that were in contact withthe agrochemicals studied presented a lower synthesis of vitellogenin mRNA, which may coincide with studies in other insectspecies. While it was postulated that this type of agrochemicals to a greater or lesser degree, depending on the familystimulates the corpora allata generating an increase in the juvenile hormone and therefore, in the case of bees, an inhibition ofthe synthesis of vitellogenin.
Fil: Sarlo, Edgardo. Universidad Nacional de Mar del Plata. Instituto de Investigaciones En Produccion, Sanidad y Ambiente. - Consejo Nacional de Investigaciones Cientificas y Tecnicas. Centro Cientifico Tecnologico Conicet - Mar del Plata. Instituto de Investigaciones En Produccion, Sanidad y Ambiente.; Argentina
Fil: Quintana, Silvina. Universidad Nacional de Mar del Plata. Instituto de Investigaciones En Produccion, Sanidad y Ambiente. - Consejo Nacional de Investigaciones Cientificas y Tecnicas. Centro Cientifico Tecnologico Conicet - Mar del Plata. Instituto de Investigaciones En Produccion, Sanidad y Ambiente.; Argentina
Fil: Perezlindo, Tamara. Fares Taie Biotecnología; Argentina
Fil: Iglesias, Azucena Elizabeth. Universidad Nacional de Mar del Plata. Instituto de Investigaciones En Produccion, Sanidad y Ambiente. - Consejo Nacional de Investigaciones Cientificas y Tecnicas. Centro Cientifico Tecnologico Conicet - Mar del Plata. Instituto de Investigaciones En Produccion, Sanidad y Ambiente.; Argentina
Fil: Iturburu, Fernando Gastón. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Mar del Plata. Instituto de Investigaciones Marinas y Costeras. Universidad Nacional de Mar del Plata. Facultad de Ciencias Exactas y Naturales. Instituto de Investigaciones Marinas y Costeras; Argentina
Fil: Menone, Mirta Lujan. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Mar del Plata. Instituto de Investigaciones Marinas y Costeras. Universidad Nacional de Mar del Plata. Facultad de Ciencias Exactas y Naturales. Instituto de Investigaciones Marinas y Costeras; Argentina
Fil: Eguaras, Martin Javier. Universidad Nacional de Mar del Plata. Instituto de Investigaciones En Produccion, Sanidad y Ambiente. - Consejo Nacional de Investigaciones Cientificas y Tecnicas. Centro Cientifico Tecnologico Conicet - Mar del Plata. Instituto de Investigaciones En Produccion, Sanidad y Ambiente.; Argentina
Fil: Medici, Sandra Karina. Universidad Nacional de Mar del Plata. Instituto de Investigaciones En Produccion, Sanidad y Ambiente. - Consejo Nacional de Investigaciones Cientificas y Tecnicas. Centro Cientifico Tecnologico Conicet - Mar del Plata. Instituto de Investigaciones En Produccion, Sanidad y Ambiente.; Argentina
48th International Apicultural Congress
Santiago de Chile
Chile
Federación Internacional de Asociaciones de Apicultura
description Agrochemicals, and particularly insecticides in sublethal doses, are widely recognized as being among the group of stressorsthat drastically reduce the fitness of insects. Particularly, Apis mellifera is known for its association with agriculture to sublethalcontact with a wide range of this group of stressors that trigger different types of stress, such as nutritional stress. Based onthis, the aim of this research is to determine if the level of total body proteins and the expression of the Vitellogenin gene inthree days emerged bees varies before the topical exposure to a sublethal dose (LD10) of the pesticides Imidacloprid.Bifenthrin and Chlorpyrifos or a combination of pairs of them. The bioassay was performed on a total of 30 A mellifera workersper treatment, which were contaminated with each pesticide or combination of them and after 24 hours were sacrificed withliquid nitrogen. Total protein content was obtained according to Bradford (1976) while Vitelogenin mRNA levels weredetermined by RT-qPCR. No significant differences were observed in the total protein content between the control group andthe individual treatment groups with bifenthrin and chlorpyrifos, however in the rest of the treatments the total protein contentwas significantly lower than the control. A significantly difference in mRNA levels was also found between individuals in theuntreated group and all of the treated groups. Within the treated groups, the mRNA expression in the group in contact withbifenthrin was significantly higher than among all other groups. It was concluded that the individuals that were in contact withthe agrochemicals studied presented a lower synthesis of vitellogenin mRNA, which may coincide with studies in other insectspecies. While it was postulated that this type of agrochemicals to a greater or lesser degree, depending on the familystimulates the corpora allata generating an increase in the juvenile hormone and therefore, in the case of bees, an inhibition ofthe synthesis of vitellogenin.
publishDate 2023
dc.date.none.fl_str_mv 2023
dc.type.none.fl_str_mv info:eu-repo/semantics/publishedVersion
info:eu-repo/semantics/conferenceObject
Congreso
Book
http://purl.org/coar/resource_type/c_5794
info:ar-repo/semantics/documentoDeConferencia
status_str publishedVersion
format conferenceObject
dc.identifier.none.fl_str_mv http://hdl.handle.net/11336/250436
Effect of the exposure with sublethal dosis to the insecticides Imidacloprid. Bifenthrin andChlorpyrifos or its combination on the total amount of body proteins and mRNA expression levels ofVitelogenin on Apis mellifera workers bees; 48th International Apicultural Congress; Santiago de Chile; Chile; 2023; 265-265
CONICET Digital
CONICET
url http://hdl.handle.net/11336/250436
identifier_str_mv Effect of the exposure with sublethal dosis to the insecticides Imidacloprid. Bifenthrin andChlorpyrifos or its combination on the total amount of body proteins and mRNA expression levels ofVitelogenin on Apis mellifera workers bees; 48th International Apicultural Congress; Santiago de Chile; Chile; 2023; 265-265
CONICET Digital
CONICET
dc.language.none.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv info:eu-repo/semantics/altIdentifier/url/https://apimondia2023.com/docs/abstract-book.pdf
dc.rights.none.fl_str_mv info:eu-repo/semantics/openAccess
https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
eu_rights_str_mv openAccess
rights_invalid_str_mv https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
dc.format.none.fl_str_mv application/pdf
application/pdf
application/pdf
dc.coverage.none.fl_str_mv Internacional
dc.publisher.none.fl_str_mv Conmark Tourism & Event Management Company
publisher.none.fl_str_mv Conmark Tourism & Event Management Company
dc.source.none.fl_str_mv reponame:CONICET Digital (CONICET)
instname:Consejo Nacional de Investigaciones Científicas y Técnicas
reponame_str CONICET Digital (CONICET)
collection CONICET Digital (CONICET)
instname_str Consejo Nacional de Investigaciones Científicas y Técnicas
repository.name.fl_str_mv CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicas
repository.mail.fl_str_mv dasensio@conicet.gov.ar; lcarlino@conicet.gov.ar
_version_ 1844613242790346752
score 13.070432

Publicaciones similares