Low-cost extraction of multifaceted biological compounds from citrus waste using enzymes from Aspergillus Niger LBM 134

Autores
Díaz, Gabriela Verónica; Coniglio, Romina Olga; Ortellado, Laura Ester; Zapata, Pedro Dario; Martinez, Maria Alejandra; Fonseca, Maria Isabel
Año de publicación
2025
Idioma
inglés
Tipo de recurso
artículo
Estado
versión publicada
Descripción
Agricultural industries search for biotechnological alternatives for waste management as they mean a significant concernand environmental challenge. More importantly, within a circular economy concept, such secondary substratescan be used to produce value-added compounds. This work is aimed at obtaining bioactive compounds from citruswaste by using a homemade enzymatic cocktail from Aspergillus niger LBM 134. The fungal enzymes were producedusing raw sugarcane bagasse as substrate, which increased the ecological sustainability and the cost-effectivenessof the bioprocess. As the most relevant enzyme of this cocktail, a β-glucosidase showed to optimally act at 50 °C,retaining up to 70% of residual activity after 72 h. By means of an optimized enzyme-assisted extraction, the crudeenzymatic cocktail produced was efficiently employed to extract the phenolics hesperetin, quinic, p-coumaric,and gallic acid, and the bioactive amino acid tryptophan from citrus waste. These assays yielded approximately112% and 30% of phenolic compounds over alkaline conventional and commercial enzyme extraction methods,respectively.
Fil: Díaz, Gabriela Verónica. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Nordeste; Argentina. Universidad Nacional de Misiones. Facultad de Ciencias Exactas Químicas y Naturales. Departamento de Bioquímica Clínica. Laboratorio de Biotecnología Molecular; Argentina
Fil: Coniglio, Romina Olga. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Nordeste; Argentina. Universidad Nacional de Misiones. Facultad de Ciencias Exactas Químicas y Naturales. Departamento de Bioquímica Clínica. Laboratorio de Biotecnología Molecular; Argentina
Fil: Ortellado, Laura Ester. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Nordeste; Argentina. Universidad Nacional de Misiones. Facultad de Ciencias Exactas Químicas y Naturales. Departamento de Bioquímica Clínica. Laboratorio de Biotecnología Molecular; Argentina
Fil: Zapata, Pedro Dario. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Nordeste; Argentina. Universidad Nacional de Misiones. Facultad de Ciencias Exactas Químicas y Naturales. Departamento de Bioquímica Clínica. Laboratorio de Biotecnología Molecular; Argentina
Fil: Martinez, Maria Alejandra. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Tucumán. Planta Piloto de Procesos Industriales Microbiológicos; Argentina
Fil: Fonseca, Maria Isabel. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Nordeste; Argentina. Universidad Nacional de Misiones. Facultad de Ciencias Exactas Químicas y Naturales. Departamento de Bioquímica Clínica. Laboratorio de Biotecnología Molecular; Argentina
Materia
Fungal enzymes
β-glucosidase
Hesperetin
Gallic acid
Quinic acid
P-coumaric acid
Tryptophan
Nivel de accesibilidad
acceso abierto
Condiciones de uso
https://creativecommons.org/licenses/by/2.5/ar/
Repositorio
CONICET Digital (CONICET)
Institución
Consejo Nacional de Investigaciones Científicas y Técnicas
OAI Identificador
oai:ri.conicet.gov.ar:11336/263337
Acceder
id CONICETDig_12a8130b862740c278b722777274706c
oai_identifier_str oai:ri.conicet.gov.ar:11336/263337
network_acronym_str CONICETDig
repository_id_str 3498
network_name_str CONICET Digital (CONICET)
spelling Low-cost extraction of multifaceted biological compounds from citrus waste using enzymes from Aspergillus Niger LBM 134Díaz, Gabriela VerónicaConiglio, Romina OlgaOrtellado, Laura EsterZapata, Pedro DarioMartinez, Maria AlejandraFonseca, Maria IsabelFungal enzymesβ-glucosidaseHesperetinGallic acidQuinic acidP-coumaric acidTryptophanhttps://purl.org/becyt/ford/3.4https://purl.org/becyt/ford/3Agricultural industries search for biotechnological alternatives for waste management as they mean a significant concernand environmental challenge. More importantly, within a circular economy concept, such secondary substratescan be used to produce value-added compounds. This work is aimed at obtaining bioactive compounds from citruswaste by using a homemade enzymatic cocktail from Aspergillus niger LBM 134. The fungal enzymes were producedusing raw sugarcane bagasse as substrate, which increased the ecological sustainability and the cost-effectivenessof the bioprocess. As the most relevant enzyme of this cocktail, a β-glucosidase showed to optimally act at 50 °C,retaining up to 70% of residual activity after 72 h. By means of an optimized enzyme-assisted extraction, the crudeenzymatic cocktail produced was efficiently employed to extract the phenolics hesperetin, quinic, p-coumaric,and gallic acid, and the bioactive amino acid tryptophan from citrus waste. These assays yielded approximately112% and 30% of phenolic compounds over alkaline conventional and commercial enzyme extraction methods,respectively.Fil: Díaz, Gabriela Verónica. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Nordeste; Argentina. Universidad Nacional de Misiones. Facultad de Ciencias Exactas Químicas y Naturales. Departamento de Bioquímica Clínica. Laboratorio de Biotecnología Molecular; ArgentinaFil: Coniglio, Romina Olga. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Nordeste; Argentina. Universidad Nacional de Misiones. Facultad de Ciencias Exactas Químicas y Naturales. Departamento de Bioquímica Clínica. Laboratorio de Biotecnología Molecular; ArgentinaFil: Ortellado, Laura Ester. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Nordeste; Argentina. Universidad Nacional de Misiones. Facultad de Ciencias Exactas Químicas y Naturales. Departamento de Bioquímica Clínica. Laboratorio de Biotecnología Molecular; ArgentinaFil: Zapata, Pedro Dario. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Nordeste; Argentina. Universidad Nacional de Misiones. Facultad de Ciencias Exactas Químicas y Naturales. Departamento de Bioquímica Clínica. Laboratorio de Biotecnología Molecular; ArgentinaFil: Martinez, Maria Alejandra. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Tucumán. Planta Piloto de Procesos Industriales Microbiológicos; ArgentinaFil: Fonseca, Maria Isabel. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Nordeste; Argentina. Universidad Nacional de Misiones. Facultad de Ciencias Exactas Químicas y Naturales. Departamento de Bioquímica Clínica. Laboratorio de Biotecnología Molecular; ArgentinaSpringer2025-02info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionhttp://purl.org/coar/resource_type/c_6501info:ar-repo/semantics/articuloapplication/pdfapplication/pdfapplication/pdfapplication/pdfapplication/pdfapplication/pdfhttp://hdl.handle.net/11336/263337Díaz, Gabriela Verónica; Coniglio, Romina Olga; Ortellado, Laura Ester; Zapata, Pedro Dario; Martinez, Maria Alejandra; et al.; Low-cost extraction of multifaceted biological compounds from citrus waste using enzymes from Aspergillus Niger LBM 134; Springer; Food Production, Processing and Nutrition; 7; 1; 2-2025; 1-122661-8974CONICET DigitalCONICETenginfo:eu-repo/semantics/altIdentifier/url/https://fppn.biomedcentral.com/articles/10.1186/s43014-024-00299-5info:eu-repo/semantics/altIdentifier/doi/10.1186/s43014-024-00299-5info:eu-repo/semantics/openAccesshttps://creativecommons.org/licenses/by/2.5/ar/reponame:CONICET Digital (CONICET)instname:Consejo Nacional de Investigaciones Científicas y Técnicas2025-10-15T14:43:03Zoai:ri.conicet.gov.ar:11336/263337instacron:CONICETInstitucionalhttp://ri.conicet.gov.ar/Organismo científico-tecnológicoNo correspondehttp://ri.conicet.gov.ar/oai/requestdasensio@conicet.gov.ar; lcarlino@conicet.gov.arArgentinaNo correspondeNo correspondeNo correspondeopendoar:34982025-10-15 14:43:03.455CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicasfalse
dc.title.none.fl_str_mv Low-cost extraction of multifaceted biological compounds from citrus waste using enzymes from Aspergillus Niger LBM 134
title Low-cost extraction of multifaceted biological compounds from citrus waste using enzymes from Aspergillus Niger LBM 134
spellingShingle Low-cost extraction of multifaceted biological compounds from citrus waste using enzymes from Aspergillus Niger LBM 134
Díaz, Gabriela Verónica
Fungal enzymes
β-glucosidase
Hesperetin
Gallic acid
Quinic acid
P-coumaric acid
Tryptophan
title_short Low-cost extraction of multifaceted biological compounds from citrus waste using enzymes from Aspergillus Niger LBM 134
title_full Low-cost extraction of multifaceted biological compounds from citrus waste using enzymes from Aspergillus Niger LBM 134
title_fullStr Low-cost extraction of multifaceted biological compounds from citrus waste using enzymes from Aspergillus Niger LBM 134
title_full_unstemmed Low-cost extraction of multifaceted biological compounds from citrus waste using enzymes from Aspergillus Niger LBM 134
title_sort Low-cost extraction of multifaceted biological compounds from citrus waste using enzymes from Aspergillus Niger LBM 134
dc.creator.none.fl_str_mv Díaz, Gabriela Verónica
Coniglio, Romina Olga
Ortellado, Laura Ester
Zapata, Pedro Dario
Martinez, Maria Alejandra
Fonseca, Maria Isabel
author Díaz, Gabriela Verónica
author_facet Díaz, Gabriela Verónica
Coniglio, Romina Olga
Ortellado, Laura Ester
Zapata, Pedro Dario
Martinez, Maria Alejandra
Fonseca, Maria Isabel
author_role author
author2 Coniglio, Romina Olga
Ortellado, Laura Ester
Zapata, Pedro Dario
Martinez, Maria Alejandra
Fonseca, Maria Isabel
author2_role author
author
author
author
author
dc.subject.none.fl_str_mv Fungal enzymes
β-glucosidase
Hesperetin
Gallic acid
Quinic acid
P-coumaric acid
Tryptophan
topic Fungal enzymes
β-glucosidase
Hesperetin
Gallic acid
Quinic acid
P-coumaric acid
Tryptophan
purl_subject.fl_str_mv https://purl.org/becyt/ford/3.4
https://purl.org/becyt/ford/3
dc.description.none.fl_txt_mv Agricultural industries search for biotechnological alternatives for waste management as they mean a significant concernand environmental challenge. More importantly, within a circular economy concept, such secondary substratescan be used to produce value-added compounds. This work is aimed at obtaining bioactive compounds from citruswaste by using a homemade enzymatic cocktail from Aspergillus niger LBM 134. The fungal enzymes were producedusing raw sugarcane bagasse as substrate, which increased the ecological sustainability and the cost-effectivenessof the bioprocess. As the most relevant enzyme of this cocktail, a β-glucosidase showed to optimally act at 50 °C,retaining up to 70% of residual activity after 72 h. By means of an optimized enzyme-assisted extraction, the crudeenzymatic cocktail produced was efficiently employed to extract the phenolics hesperetin, quinic, p-coumaric,and gallic acid, and the bioactive amino acid tryptophan from citrus waste. These assays yielded approximately112% and 30% of phenolic compounds over alkaline conventional and commercial enzyme extraction methods,respectively.
Fil: Díaz, Gabriela Verónica. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Nordeste; Argentina. Universidad Nacional de Misiones. Facultad de Ciencias Exactas Químicas y Naturales. Departamento de Bioquímica Clínica. Laboratorio de Biotecnología Molecular; Argentina
Fil: Coniglio, Romina Olga. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Nordeste; Argentina. Universidad Nacional de Misiones. Facultad de Ciencias Exactas Químicas y Naturales. Departamento de Bioquímica Clínica. Laboratorio de Biotecnología Molecular; Argentina
Fil: Ortellado, Laura Ester. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Nordeste; Argentina. Universidad Nacional de Misiones. Facultad de Ciencias Exactas Químicas y Naturales. Departamento de Bioquímica Clínica. Laboratorio de Biotecnología Molecular; Argentina
Fil: Zapata, Pedro Dario. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Nordeste; Argentina. Universidad Nacional de Misiones. Facultad de Ciencias Exactas Químicas y Naturales. Departamento de Bioquímica Clínica. Laboratorio de Biotecnología Molecular; Argentina
Fil: Martinez, Maria Alejandra. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Tucumán. Planta Piloto de Procesos Industriales Microbiológicos; Argentina
Fil: Fonseca, Maria Isabel. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Nordeste; Argentina. Universidad Nacional de Misiones. Facultad de Ciencias Exactas Químicas y Naturales. Departamento de Bioquímica Clínica. Laboratorio de Biotecnología Molecular; Argentina
description Agricultural industries search for biotechnological alternatives for waste management as they mean a significant concernand environmental challenge. More importantly, within a circular economy concept, such secondary substratescan be used to produce value-added compounds. This work is aimed at obtaining bioactive compounds from citruswaste by using a homemade enzymatic cocktail from Aspergillus niger LBM 134. The fungal enzymes were producedusing raw sugarcane bagasse as substrate, which increased the ecological sustainability and the cost-effectivenessof the bioprocess. As the most relevant enzyme of this cocktail, a β-glucosidase showed to optimally act at 50 °C,retaining up to 70% of residual activity after 72 h. By means of an optimized enzyme-assisted extraction, the crudeenzymatic cocktail produced was efficiently employed to extract the phenolics hesperetin, quinic, p-coumaric,and gallic acid, and the bioactive amino acid tryptophan from citrus waste. These assays yielded approximately112% and 30% of phenolic compounds over alkaline conventional and commercial enzyme extraction methods,respectively.
publishDate 2025
dc.date.none.fl_str_mv 2025-02
dc.type.none.fl_str_mv info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
http://purl.org/coar/resource_type/c_6501
info:ar-repo/semantics/articulo
format article
status_str publishedVersion
dc.identifier.none.fl_str_mv http://hdl.handle.net/11336/263337
Díaz, Gabriela Verónica; Coniglio, Romina Olga; Ortellado, Laura Ester; Zapata, Pedro Dario; Martinez, Maria Alejandra; et al.; Low-cost extraction of multifaceted biological compounds from citrus waste using enzymes from Aspergillus Niger LBM 134; Springer; Food Production, Processing and Nutrition; 7; 1; 2-2025; 1-12
2661-8974
CONICET Digital
CONICET
url http://hdl.handle.net/11336/263337
identifier_str_mv Díaz, Gabriela Verónica; Coniglio, Romina Olga; Ortellado, Laura Ester; Zapata, Pedro Dario; Martinez, Maria Alejandra; et al.; Low-cost extraction of multifaceted biological compounds from citrus waste using enzymes from Aspergillus Niger LBM 134; Springer; Food Production, Processing and Nutrition; 7; 1; 2-2025; 1-12
2661-8974
CONICET Digital
CONICET
dc.language.none.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv info:eu-repo/semantics/altIdentifier/url/https://fppn.biomedcentral.com/articles/10.1186/s43014-024-00299-5
info:eu-repo/semantics/altIdentifier/doi/10.1186/s43014-024-00299-5
dc.rights.none.fl_str_mv info:eu-repo/semantics/openAccess
https://creativecommons.org/licenses/by/2.5/ar/
eu_rights_str_mv openAccess
rights_invalid_str_mv https://creativecommons.org/licenses/by/2.5/ar/
dc.format.none.fl_str_mv application/pdf
application/pdf
application/pdf
application/pdf
application/pdf
application/pdf
dc.publisher.none.fl_str_mv Springer
publisher.none.fl_str_mv Springer
dc.source.none.fl_str_mv reponame:CONICET Digital (CONICET)
instname:Consejo Nacional de Investigaciones Científicas y Técnicas
reponame_str CONICET Digital (CONICET)
collection CONICET Digital (CONICET)
instname_str Consejo Nacional de Investigaciones Científicas y Técnicas
repository.name.fl_str_mv CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicas
repository.mail.fl_str_mv dasensio@conicet.gov.ar; lcarlino@conicet.gov.ar
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score 13.22299

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