Minimizing the time and cost of production of transgenic alfalfa libraries using the highly efficient completely sequenced vector pPZP200BAR

Autores
Jozefkowicz, Cintia; Bottero, Ana Emilia; Pascuan, Cecilia Gabriela; Pagano, Elba Maria; Ayub, Nicolás Daniel; Soto, Gabriela Cynthia
Año de publicación
2016
Idioma
inglés
Tipo de recurso
artículo
Estado
versión publicada
Descripción
Alfalfa is the most important forage legume worldwide. However, similar to other minorforage crops, it is usually harvested along with weeds, which decrease its nutrient qualityand thus reduce its high value in the market. In addition, weeds reduce alfalfa yield byabout 50%. Although weeds are the limiting factor for alfalfa production, little progress hasbeen made in the incorporation of herbicide-tolerant traits into commercial alfalfa. This ispartially due to the high times and costs needed for the production of vast numbers oftransgenic alfalfa events as an empirical approach to bypass the random transgenicsilencing and for the identification of an event with optimal transgene expression. In thisfocus article, we report the complete sequence of pPZP200BAR and the extremely highefficiency of this binary vector in alfalfa transformation, opening the way for rapid andinexpensive production of transgenic events for alfalfa improvement public programs.
Fil: Jozefkowicz, Cintia. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina. Instituto Nacional de Tecnología Agropecuaria. Centro de Investigación en Ciencias Veterinarias y Agronómicas; Argentina
Fil: Bottero, Ana Emilia. Instituto Nacional de Tecnología Agropecuaria. Centro de Investigación en Ciencias Veterinarias y Agronómicas; Argentina
Fil: Pascuan, Cecilia Gabriela. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina. Instituto Nacional de Tecnología Agropecuaria. Centro de Investigación en Ciencias Veterinarias y Agronómicas; Argentina
Fil: Pagano, Elba Maria. Instituto Nacional de Tecnología Agropecuaria. Centro de Investigación en Ciencias Veterinarias y Agronómicas; Argentina
Fil: Ayub, Nicolás Daniel. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina. Instituto Nacional de Tecnología Agropecuaria. Centro de Investigación en Ciencias Veterinarias y Agronómicas; Argentina
Fil: Soto, Gabriela Cynthia. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina. Instituto Nacional de Tecnología Agropecuaria. Centro de Investigación en Ciencias Veterinarias y Agronómicas; Argentina
Materia
Ppzp200bar
Complete Sequence
Binary Vector
Alfalfa
Transformation
Nivel de accesibilidad
acceso abierto
Condiciones de uso
https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
Repositorio
CONICET Digital (CONICET)
Institución
Consejo Nacional de Investigaciones Científicas y Técnicas
OAI Identificador
oai:ri.conicet.gov.ar:11336/46995
Acceder
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oai_identifier_str oai:ri.conicet.gov.ar:11336/46995
network_acronym_str CONICETDig
repository_id_str 3498
network_name_str CONICET Digital (CONICET)
spelling Minimizing the time and cost of production of transgenic alfalfa libraries using the highly efficient completely sequenced vector pPZP200BARJozefkowicz, CintiaBottero, Ana EmiliaPascuan, Cecilia GabrielaPagano, Elba MariaAyub, Nicolás DanielSoto, Gabriela CynthiaPpzp200barComplete SequenceBinary VectorAlfalfaTransformationhttps://purl.org/becyt/ford/1.6https://purl.org/becyt/ford/1Alfalfa is the most important forage legume worldwide. However, similar to other minorforage crops, it is usually harvested along with weeds, which decrease its nutrient qualityand thus reduce its high value in the market. In addition, weeds reduce alfalfa yield byabout 50%. Although weeds are the limiting factor for alfalfa production, little progress hasbeen made in the incorporation of herbicide-tolerant traits into commercial alfalfa. This ispartially due to the high times and costs needed for the production of vast numbers oftransgenic alfalfa events as an empirical approach to bypass the random transgenicsilencing and for the identification of an event with optimal transgene expression. In thisfocus article, we report the complete sequence of pPZP200BAR and the extremely highefficiency of this binary vector in alfalfa transformation, opening the way for rapid andinexpensive production of transgenic events for alfalfa improvement public programs.Fil: Jozefkowicz, Cintia. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina. Instituto Nacional de Tecnología Agropecuaria. Centro de Investigación en Ciencias Veterinarias y Agronómicas; ArgentinaFil: Bottero, Ana Emilia. Instituto Nacional de Tecnología Agropecuaria. Centro de Investigación en Ciencias Veterinarias y Agronómicas; ArgentinaFil: Pascuan, Cecilia Gabriela. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina. Instituto Nacional de Tecnología Agropecuaria. Centro de Investigación en Ciencias Veterinarias y Agronómicas; ArgentinaFil: Pagano, Elba Maria. Instituto Nacional de Tecnología Agropecuaria. Centro de Investigación en Ciencias Veterinarias y Agronómicas; ArgentinaFil: Ayub, Nicolás Daniel. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina. Instituto Nacional de Tecnología Agropecuaria. Centro de Investigación en Ciencias Veterinarias y Agronómicas; ArgentinaFil: Soto, Gabriela Cynthia. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina. Instituto Nacional de Tecnología Agropecuaria. Centro de Investigación en Ciencias Veterinarias y Agronómicas; ArgentinaSpringer2016-07info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionhttp://purl.org/coar/resource_type/c_6501info:ar-repo/semantics/articuloapplication/pdfapplication/pdfhttp://hdl.handle.net/11336/46995Jozefkowicz, Cintia; Bottero, Ana Emilia; Pascuan, Cecilia Gabriela; Pagano, Elba Maria; Ayub, Nicolás Daniel; et al.; Minimizing the time and cost of production of transgenic alfalfa libraries using the highly efficient completely sequenced vector pPZP200BAR; Springer; Plant Cell Reports; 35; 9; 7-2016; 1987-19900721-7714CONICET DigitalCONICETenginfo:eu-repo/semantics/altIdentifier/doi/10.1007/s00299-016-2026-7info:eu-repo/semantics/altIdentifier/url/https://link.springer.com/article/10.1007%2Fs00299-016-2026-7info:eu-repo/semantics/openAccesshttps://creativecommons.org/licenses/by-nc-sa/2.5/ar/reponame:CONICET Digital (CONICET)instname:Consejo Nacional de Investigaciones Científicas y Técnicas2025-09-17T11:56:44Zoai:ri.conicet.gov.ar:11336/46995instacron:CONICETInstitucionalhttp://ri.conicet.gov.ar/Organismo científico-tecnológicoNo correspondehttp://ri.conicet.gov.ar/oai/requestdasensio@conicet.gov.ar; lcarlino@conicet.gov.arArgentinaNo correspondeNo correspondeNo correspondeopendoar:34982025-09-17 11:56:45.038CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicasfalse
dc.title.none.fl_str_mv Minimizing the time and cost of production of transgenic alfalfa libraries using the highly efficient completely sequenced vector pPZP200BAR
title Minimizing the time and cost of production of transgenic alfalfa libraries using the highly efficient completely sequenced vector pPZP200BAR
spellingShingle Minimizing the time and cost of production of transgenic alfalfa libraries using the highly efficient completely sequenced vector pPZP200BAR
Jozefkowicz, Cintia
Ppzp200bar
Complete Sequence
Binary Vector
Alfalfa
Transformation
title_short Minimizing the time and cost of production of transgenic alfalfa libraries using the highly efficient completely sequenced vector pPZP200BAR
title_full Minimizing the time and cost of production of transgenic alfalfa libraries using the highly efficient completely sequenced vector pPZP200BAR
title_fullStr Minimizing the time and cost of production of transgenic alfalfa libraries using the highly efficient completely sequenced vector pPZP200BAR
title_full_unstemmed Minimizing the time and cost of production of transgenic alfalfa libraries using the highly efficient completely sequenced vector pPZP200BAR
title_sort Minimizing the time and cost of production of transgenic alfalfa libraries using the highly efficient completely sequenced vector pPZP200BAR
dc.creator.none.fl_str_mv Jozefkowicz, Cintia
Bottero, Ana Emilia
Pascuan, Cecilia Gabriela
Pagano, Elba Maria
Ayub, Nicolás Daniel
Soto, Gabriela Cynthia
author Jozefkowicz, Cintia
author_facet Jozefkowicz, Cintia
Bottero, Ana Emilia
Pascuan, Cecilia Gabriela
Pagano, Elba Maria
Ayub, Nicolás Daniel
Soto, Gabriela Cynthia
author_role author
author2 Bottero, Ana Emilia
Pascuan, Cecilia Gabriela
Pagano, Elba Maria
Ayub, Nicolás Daniel
Soto, Gabriela Cynthia
author2_role author
author
author
author
author
dc.subject.none.fl_str_mv Ppzp200bar
Complete Sequence
Binary Vector
Alfalfa
Transformation
topic Ppzp200bar
Complete Sequence
Binary Vector
Alfalfa
Transformation
purl_subject.fl_str_mv https://purl.org/becyt/ford/1.6
https://purl.org/becyt/ford/1
dc.description.none.fl_txt_mv Alfalfa is the most important forage legume worldwide. However, similar to other minorforage crops, it is usually harvested along with weeds, which decrease its nutrient qualityand thus reduce its high value in the market. In addition, weeds reduce alfalfa yield byabout 50%. Although weeds are the limiting factor for alfalfa production, little progress hasbeen made in the incorporation of herbicide-tolerant traits into commercial alfalfa. This ispartially due to the high times and costs needed for the production of vast numbers oftransgenic alfalfa events as an empirical approach to bypass the random transgenicsilencing and for the identification of an event with optimal transgene expression. In thisfocus article, we report the complete sequence of pPZP200BAR and the extremely highefficiency of this binary vector in alfalfa transformation, opening the way for rapid andinexpensive production of transgenic events for alfalfa improvement public programs.
Fil: Jozefkowicz, Cintia. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina. Instituto Nacional de Tecnología Agropecuaria. Centro de Investigación en Ciencias Veterinarias y Agronómicas; Argentina
Fil: Bottero, Ana Emilia. Instituto Nacional de Tecnología Agropecuaria. Centro de Investigación en Ciencias Veterinarias y Agronómicas; Argentina
Fil: Pascuan, Cecilia Gabriela. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina. Instituto Nacional de Tecnología Agropecuaria. Centro de Investigación en Ciencias Veterinarias y Agronómicas; Argentina
Fil: Pagano, Elba Maria. Instituto Nacional de Tecnología Agropecuaria. Centro de Investigación en Ciencias Veterinarias y Agronómicas; Argentina
Fil: Ayub, Nicolás Daniel. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina. Instituto Nacional de Tecnología Agropecuaria. Centro de Investigación en Ciencias Veterinarias y Agronómicas; Argentina
Fil: Soto, Gabriela Cynthia. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina. Instituto Nacional de Tecnología Agropecuaria. Centro de Investigación en Ciencias Veterinarias y Agronómicas; Argentina
description Alfalfa is the most important forage legume worldwide. However, similar to other minorforage crops, it is usually harvested along with weeds, which decrease its nutrient qualityand thus reduce its high value in the market. In addition, weeds reduce alfalfa yield byabout 50%. Although weeds are the limiting factor for alfalfa production, little progress hasbeen made in the incorporation of herbicide-tolerant traits into commercial alfalfa. This ispartially due to the high times and costs needed for the production of vast numbers oftransgenic alfalfa events as an empirical approach to bypass the random transgenicsilencing and for the identification of an event with optimal transgene expression. In thisfocus article, we report the complete sequence of pPZP200BAR and the extremely highefficiency of this binary vector in alfalfa transformation, opening the way for rapid andinexpensive production of transgenic events for alfalfa improvement public programs.
publishDate 2016
dc.date.none.fl_str_mv 2016-07
dc.type.none.fl_str_mv info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
http://purl.org/coar/resource_type/c_6501
info:ar-repo/semantics/articulo
format article
status_str publishedVersion
dc.identifier.none.fl_str_mv http://hdl.handle.net/11336/46995
Jozefkowicz, Cintia; Bottero, Ana Emilia; Pascuan, Cecilia Gabriela; Pagano, Elba Maria; Ayub, Nicolás Daniel; et al.; Minimizing the time and cost of production of transgenic alfalfa libraries using the highly efficient completely sequenced vector pPZP200BAR; Springer; Plant Cell Reports; 35; 9; 7-2016; 1987-1990
0721-7714
CONICET Digital
CONICET
url http://hdl.handle.net/11336/46995
identifier_str_mv Jozefkowicz, Cintia; Bottero, Ana Emilia; Pascuan, Cecilia Gabriela; Pagano, Elba Maria; Ayub, Nicolás Daniel; et al.; Minimizing the time and cost of production of transgenic alfalfa libraries using the highly efficient completely sequenced vector pPZP200BAR; Springer; Plant Cell Reports; 35; 9; 7-2016; 1987-1990
0721-7714
CONICET Digital
CONICET
dc.language.none.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv info:eu-repo/semantics/altIdentifier/doi/10.1007/s00299-016-2026-7
info:eu-repo/semantics/altIdentifier/url/https://link.springer.com/article/10.1007%2Fs00299-016-2026-7
dc.rights.none.fl_str_mv info:eu-repo/semantics/openAccess
https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
eu_rights_str_mv openAccess
rights_invalid_str_mv https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
dc.format.none.fl_str_mv application/pdf
application/pdf
dc.publisher.none.fl_str_mv Springer
publisher.none.fl_str_mv Springer
dc.source.none.fl_str_mv reponame:CONICET Digital (CONICET)
instname:Consejo Nacional de Investigaciones Científicas y Técnicas
reponame_str CONICET Digital (CONICET)
collection CONICET Digital (CONICET)
instname_str Consejo Nacional de Investigaciones Científicas y Técnicas
repository.name.fl_str_mv CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicas
repository.mail.fl_str_mv dasensio@conicet.gov.ar; lcarlino@conicet.gov.ar
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score 13.001348

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