Overexpression of hyaluronan synthase 2 and gonadotropin receptors in cumulus cells of goats subjected to one-shot eCG/FSH hormonal treatment for ovarian stimulation

Autores
Santos, Juliana D. R.; Batista, Ribrio I. T. P.; Magalhães, Livia C.; Paula Jr., Alexander R.; Souza, Samara S.; Salamone, Daniel Felipe; Bhat, Maajid H.; Teixeira, Dárcio I. A.; Freitas, Vicente J. F.; Melo, Luciana M.
Año de publicación
2016
Idioma
inglés
Tipo de recurso
artículo
Estado
versión publicada
Descripción
Hormonal ovarian stimulation may affect transcripts in somatic cells of cumulus-oocyte complexes (COCs) and affect the resulting oocyte quality. Here, in parallel with morphological classification and in vitro maturation (IVM) rate analysis, we investigated the expression of hyaluronan synthase 2 (HAS2), gonadotropic receptors (FSHR and LHR) and connexin 43 (GJA1) in cumulus cells (CCs) from goat COCs after multi-dose FSH (MD) or one-shot FSH/eCG (OS) treatments, using bovine COCs as control groups. The MD treatment produced more large follicles, and the resulting COCs had a better morphology and IVM rate than were obtained with OS. The OS treatment produced COCs with increased HAS2, FSHR, LHR and GJA1 expression. This gene expression pattern was also observed in the CCs of COCs that showed poor morphological characteristics. On the other hand, the mRNA levels were more similar between groups after IVM; FSHR and LHR were the main genes that showed decreased expression. Some events that occurred in bovine CCs during IVM, such as cell expansion, increased HAS2 expression and decreased GJA1 expression, were less evident or did not occur in goat COCs. In conclusion, increasing HAS2, FSHR, LHR and GJA1 expression in goat COCs does not confer greater meiotic competence to oocytes. Instead, it may result from poor regulation of gene expression in CCs by lower quality oocytes. Finally, cumulus expansion, together with HAS2 upregulation and GJA1 downregulation, seems to be more important for bovine COCs than for goat COCs. Additional studies are needed to investigate the importance of other HAS isoforms and connexins in goat COCs.
Fil: Santos, Juliana D. R.. Universidade Estadual do Ceará; Brasil
Fil: Batista, Ribrio I. T. P.. Universidade Estadual do Ceará; Brasil
Fil: Magalhães, Livia C.. Universidade Estadual do Ceará; Brasil
Fil: Paula Jr., Alexander R.. Universidade Estadual do Ceará; Brasil
Fil: Souza, Samara S.. Universidade Estadual do Ceará; Brasil
Fil: Salamone, Daniel Felipe. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Parque Centenario. Unidad Ejecutora de Investigaciones en Producción Animal. Universidad de Buenos Aires. Facultad de Ciencias Veterinarias. Unidad Ejecutora de Investigaciones en Producción Animal; Argentina
Fil: Bhat, Maajid H.. Universidade Estadual do Ceará; Brasil
Fil: Teixeira, Dárcio I. A.. Universidade Estadual do Ceará; Brasil
Fil: Freitas, Vicente J. F.. Universidade Estadual do Ceará; Brasil
Fil: Melo, Luciana M.. Universidade Estadual do Ceará; Brasil
Materia
Connexin 43
Cumulus Cell
Fshr
Has2
Lhr
Oocyte Maturation
Nivel de accesibilidad
acceso abierto
Condiciones de uso
https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
Repositorio
CONICET Digital (CONICET)
Institución
Consejo Nacional de Investigaciones Científicas y Técnicas
OAI Identificador
oai:ri.conicet.gov.ar:11336/51089
Acceder
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oai_identifier_str oai:ri.conicet.gov.ar:11336/51089
network_acronym_str CONICETDig
repository_id_str 3498
network_name_str CONICET Digital (CONICET)
spelling Overexpression of hyaluronan synthase 2 and gonadotropin receptors in cumulus cells of goats subjected to one-shot eCG/FSH hormonal treatment for ovarian stimulationSantos, Juliana D. R.Batista, Ribrio I. T. P.Magalhães, Livia C.Paula Jr., Alexander R.Souza, Samara S.Salamone, Daniel FelipeBhat, Maajid H.Teixeira, Dárcio I. A.Freitas, Vicente J. F.Melo, Luciana M.Connexin 43Cumulus CellFshrHas2LhrOocyte Maturationhttps://purl.org/becyt/ford/4.4https://purl.org/becyt/ford/4Hormonal ovarian stimulation may affect transcripts in somatic cells of cumulus-oocyte complexes (COCs) and affect the resulting oocyte quality. Here, in parallel with morphological classification and in vitro maturation (IVM) rate analysis, we investigated the expression of hyaluronan synthase 2 (HAS2), gonadotropic receptors (FSHR and LHR) and connexin 43 (GJA1) in cumulus cells (CCs) from goat COCs after multi-dose FSH (MD) or one-shot FSH/eCG (OS) treatments, using bovine COCs as control groups. The MD treatment produced more large follicles, and the resulting COCs had a better morphology and IVM rate than were obtained with OS. The OS treatment produced COCs with increased HAS2, FSHR, LHR and GJA1 expression. This gene expression pattern was also observed in the CCs of COCs that showed poor morphological characteristics. On the other hand, the mRNA levels were more similar between groups after IVM; FSHR and LHR were the main genes that showed decreased expression. Some events that occurred in bovine CCs during IVM, such as cell expansion, increased HAS2 expression and decreased GJA1 expression, were less evident or did not occur in goat COCs. In conclusion, increasing HAS2, FSHR, LHR and GJA1 expression in goat COCs does not confer greater meiotic competence to oocytes. Instead, it may result from poor regulation of gene expression in CCs by lower quality oocytes. Finally, cumulus expansion, together with HAS2 upregulation and GJA1 downregulation, seems to be more important for bovine COCs than for goat COCs. Additional studies are needed to investigate the importance of other HAS isoforms and connexins in goat COCs.Fil: Santos, Juliana D. R.. Universidade Estadual do Ceará; BrasilFil: Batista, Ribrio I. T. P.. Universidade Estadual do Ceará; BrasilFil: Magalhães, Livia C.. Universidade Estadual do Ceará; BrasilFil: Paula Jr., Alexander R.. Universidade Estadual do Ceará; BrasilFil: Souza, Samara S.. Universidade Estadual do Ceará; BrasilFil: Salamone, Daniel Felipe. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Parque Centenario. Unidad Ejecutora de Investigaciones en Producción Animal. Universidad de Buenos Aires. Facultad de Ciencias Veterinarias. Unidad Ejecutora de Investigaciones en Producción Animal; ArgentinaFil: Bhat, Maajid H.. Universidade Estadual do Ceará; BrasilFil: Teixeira, Dárcio I. A.. Universidade Estadual do Ceará; BrasilFil: Freitas, Vicente J. F.. Universidade Estadual do Ceará; BrasilFil: Melo, Luciana M.. Universidade Estadual do Ceará; BrasilElsevier Science2016-07info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionhttp://purl.org/coar/resource_type/c_6501info:ar-repo/semantics/articuloapplication/pdfapplication/pdfhttp://hdl.handle.net/11336/51089Santos, Juliana D. R.; Batista, Ribrio I. T. P.; Magalhães, Livia C.; Paula Jr., Alexander R.; Souza, Samara S.; et al.; Overexpression of hyaluronan synthase 2 and gonadotropin receptors in cumulus cells of goats subjected to one-shot eCG/FSH hormonal treatment for ovarian stimulation; Elsevier Science; Animal Reproduction Science; 170; 7-2016; 15-240378-43201873-2232CONICET DigitalCONICETenginfo:eu-repo/semantics/altIdentifier/doi/10.1016/j.anireprosci.2016.03.008info:eu-repo/semantics/altIdentifier/url/https://www.sciencedirect.com/science/article/pii/S037843201630094Xinfo:eu-repo/semantics/openAccesshttps://creativecommons.org/licenses/by-nc-sa/2.5/ar/reponame:CONICET Digital (CONICET)instname:Consejo Nacional de Investigaciones Científicas y Técnicas2025-09-29T10:26:04Zoai:ri.conicet.gov.ar:11336/51089instacron:CONICETInstitucionalhttp://ri.conicet.gov.ar/Organismo científico-tecnológicoNo correspondehttp://ri.conicet.gov.ar/oai/requestdasensio@conicet.gov.ar; lcarlino@conicet.gov.arArgentinaNo correspondeNo correspondeNo correspondeopendoar:34982025-09-29 10:26:04.308CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicasfalse
dc.title.none.fl_str_mv Overexpression of hyaluronan synthase 2 and gonadotropin receptors in cumulus cells of goats subjected to one-shot eCG/FSH hormonal treatment for ovarian stimulation
title Overexpression of hyaluronan synthase 2 and gonadotropin receptors in cumulus cells of goats subjected to one-shot eCG/FSH hormonal treatment for ovarian stimulation
spellingShingle Overexpression of hyaluronan synthase 2 and gonadotropin receptors in cumulus cells of goats subjected to one-shot eCG/FSH hormonal treatment for ovarian stimulation
Santos, Juliana D. R.
Connexin 43
Cumulus Cell
Fshr
Has2
Lhr
Oocyte Maturation
title_short Overexpression of hyaluronan synthase 2 and gonadotropin receptors in cumulus cells of goats subjected to one-shot eCG/FSH hormonal treatment for ovarian stimulation
title_full Overexpression of hyaluronan synthase 2 and gonadotropin receptors in cumulus cells of goats subjected to one-shot eCG/FSH hormonal treatment for ovarian stimulation
title_fullStr Overexpression of hyaluronan synthase 2 and gonadotropin receptors in cumulus cells of goats subjected to one-shot eCG/FSH hormonal treatment for ovarian stimulation
title_full_unstemmed Overexpression of hyaluronan synthase 2 and gonadotropin receptors in cumulus cells of goats subjected to one-shot eCG/FSH hormonal treatment for ovarian stimulation
title_sort Overexpression of hyaluronan synthase 2 and gonadotropin receptors in cumulus cells of goats subjected to one-shot eCG/FSH hormonal treatment for ovarian stimulation
dc.creator.none.fl_str_mv Santos, Juliana D. R.
Batista, Ribrio I. T. P.
Magalhães, Livia C.
Paula Jr., Alexander R.
Souza, Samara S.
Salamone, Daniel Felipe
Bhat, Maajid H.
Teixeira, Dárcio I. A.
Freitas, Vicente J. F.
Melo, Luciana M.
author Santos, Juliana D. R.
author_facet Santos, Juliana D. R.
Batista, Ribrio I. T. P.
Magalhães, Livia C.
Paula Jr., Alexander R.
Souza, Samara S.
Salamone, Daniel Felipe
Bhat, Maajid H.
Teixeira, Dárcio I. A.
Freitas, Vicente J. F.
Melo, Luciana M.
author_role author
author2 Batista, Ribrio I. T. P.
Magalhães, Livia C.
Paula Jr., Alexander R.
Souza, Samara S.
Salamone, Daniel Felipe
Bhat, Maajid H.
Teixeira, Dárcio I. A.
Freitas, Vicente J. F.
Melo, Luciana M.
author2_role author
author
author
author
author
author
author
author
author
dc.subject.none.fl_str_mv Connexin 43
Cumulus Cell
Fshr
Has2
Lhr
Oocyte Maturation
topic Connexin 43
Cumulus Cell
Fshr
Has2
Lhr
Oocyte Maturation
purl_subject.fl_str_mv https://purl.org/becyt/ford/4.4
https://purl.org/becyt/ford/4
dc.description.none.fl_txt_mv Hormonal ovarian stimulation may affect transcripts in somatic cells of cumulus-oocyte complexes (COCs) and affect the resulting oocyte quality. Here, in parallel with morphological classification and in vitro maturation (IVM) rate analysis, we investigated the expression of hyaluronan synthase 2 (HAS2), gonadotropic receptors (FSHR and LHR) and connexin 43 (GJA1) in cumulus cells (CCs) from goat COCs after multi-dose FSH (MD) or one-shot FSH/eCG (OS) treatments, using bovine COCs as control groups. The MD treatment produced more large follicles, and the resulting COCs had a better morphology and IVM rate than were obtained with OS. The OS treatment produced COCs with increased HAS2, FSHR, LHR and GJA1 expression. This gene expression pattern was also observed in the CCs of COCs that showed poor morphological characteristics. On the other hand, the mRNA levels were more similar between groups after IVM; FSHR and LHR were the main genes that showed decreased expression. Some events that occurred in bovine CCs during IVM, such as cell expansion, increased HAS2 expression and decreased GJA1 expression, were less evident or did not occur in goat COCs. In conclusion, increasing HAS2, FSHR, LHR and GJA1 expression in goat COCs does not confer greater meiotic competence to oocytes. Instead, it may result from poor regulation of gene expression in CCs by lower quality oocytes. Finally, cumulus expansion, together with HAS2 upregulation and GJA1 downregulation, seems to be more important for bovine COCs than for goat COCs. Additional studies are needed to investigate the importance of other HAS isoforms and connexins in goat COCs.
Fil: Santos, Juliana D. R.. Universidade Estadual do Ceará; Brasil
Fil: Batista, Ribrio I. T. P.. Universidade Estadual do Ceará; Brasil
Fil: Magalhães, Livia C.. Universidade Estadual do Ceará; Brasil
Fil: Paula Jr., Alexander R.. Universidade Estadual do Ceará; Brasil
Fil: Souza, Samara S.. Universidade Estadual do Ceará; Brasil
Fil: Salamone, Daniel Felipe. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Parque Centenario. Unidad Ejecutora de Investigaciones en Producción Animal. Universidad de Buenos Aires. Facultad de Ciencias Veterinarias. Unidad Ejecutora de Investigaciones en Producción Animal; Argentina
Fil: Bhat, Maajid H.. Universidade Estadual do Ceará; Brasil
Fil: Teixeira, Dárcio I. A.. Universidade Estadual do Ceará; Brasil
Fil: Freitas, Vicente J. F.. Universidade Estadual do Ceará; Brasil
Fil: Melo, Luciana M.. Universidade Estadual do Ceará; Brasil
description Hormonal ovarian stimulation may affect transcripts in somatic cells of cumulus-oocyte complexes (COCs) and affect the resulting oocyte quality. Here, in parallel with morphological classification and in vitro maturation (IVM) rate analysis, we investigated the expression of hyaluronan synthase 2 (HAS2), gonadotropic receptors (FSHR and LHR) and connexin 43 (GJA1) in cumulus cells (CCs) from goat COCs after multi-dose FSH (MD) or one-shot FSH/eCG (OS) treatments, using bovine COCs as control groups. The MD treatment produced more large follicles, and the resulting COCs had a better morphology and IVM rate than were obtained with OS. The OS treatment produced COCs with increased HAS2, FSHR, LHR and GJA1 expression. This gene expression pattern was also observed in the CCs of COCs that showed poor morphological characteristics. On the other hand, the mRNA levels were more similar between groups after IVM; FSHR and LHR were the main genes that showed decreased expression. Some events that occurred in bovine CCs during IVM, such as cell expansion, increased HAS2 expression and decreased GJA1 expression, were less evident or did not occur in goat COCs. In conclusion, increasing HAS2, FSHR, LHR and GJA1 expression in goat COCs does not confer greater meiotic competence to oocytes. Instead, it may result from poor regulation of gene expression in CCs by lower quality oocytes. Finally, cumulus expansion, together with HAS2 upregulation and GJA1 downregulation, seems to be more important for bovine COCs than for goat COCs. Additional studies are needed to investigate the importance of other HAS isoforms and connexins in goat COCs.
publishDate 2016
dc.date.none.fl_str_mv 2016-07
dc.type.none.fl_str_mv info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
http://purl.org/coar/resource_type/c_6501
info:ar-repo/semantics/articulo
format article
status_str publishedVersion
dc.identifier.none.fl_str_mv http://hdl.handle.net/11336/51089
Santos, Juliana D. R.; Batista, Ribrio I. T. P.; Magalhães, Livia C.; Paula Jr., Alexander R.; Souza, Samara S.; et al.; Overexpression of hyaluronan synthase 2 and gonadotropin receptors in cumulus cells of goats subjected to one-shot eCG/FSH hormonal treatment for ovarian stimulation; Elsevier Science; Animal Reproduction Science; 170; 7-2016; 15-24
0378-4320
1873-2232
CONICET Digital
CONICET
url http://hdl.handle.net/11336/51089
identifier_str_mv Santos, Juliana D. R.; Batista, Ribrio I. T. P.; Magalhães, Livia C.; Paula Jr., Alexander R.; Souza, Samara S.; et al.; Overexpression of hyaluronan synthase 2 and gonadotropin receptors in cumulus cells of goats subjected to one-shot eCG/FSH hormonal treatment for ovarian stimulation; Elsevier Science; Animal Reproduction Science; 170; 7-2016; 15-24
0378-4320
1873-2232
CONICET Digital
CONICET
dc.language.none.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv info:eu-repo/semantics/altIdentifier/doi/10.1016/j.anireprosci.2016.03.008
info:eu-repo/semantics/altIdentifier/url/https://www.sciencedirect.com/science/article/pii/S037843201630094X
dc.rights.none.fl_str_mv info:eu-repo/semantics/openAccess
https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
eu_rights_str_mv openAccess
rights_invalid_str_mv https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
dc.format.none.fl_str_mv application/pdf
application/pdf
dc.publisher.none.fl_str_mv Elsevier Science
publisher.none.fl_str_mv Elsevier Science
dc.source.none.fl_str_mv reponame:CONICET Digital (CONICET)
instname:Consejo Nacional de Investigaciones Científicas y Técnicas
reponame_str CONICET Digital (CONICET)
collection CONICET Digital (CONICET)
instname_str Consejo Nacional de Investigaciones Científicas y Técnicas
repository.name.fl_str_mv CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicas
repository.mail.fl_str_mv dasensio@conicet.gov.ar; lcarlino@conicet.gov.ar
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score 13.070432

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